Targeting PNPO to suppress tumor growth via inhibiting autophagic flux and to reverse paclitaxel resistance in ovarian cancer
In conclusion, PNPO has a regulatory effect on lysosomal biogenesis that in turn promotes autophagic flux, leadin g to OC cell proliferation, and tumor formation, and is a paclitaxel-resistant factor. These data imply a potential application by targeting PNPO to suppress tumor growth and reverse PTX resistance in OC. (Source: Apoptosis)
Source: Apoptosis - April 13, 2024 Category: Molecular Biology Source Type: research
Validating nutrient selection for product-group-specific nutrient indices for use as functional units in life cycle assessment of foods
Br J Nutr. 2024 Apr 12:1-23. doi: 10.1017/S0007114524000709. Online ahead of print.ABSTRACTThe ability to provide adequate nutrition is considered a key factor in evaluating the sustainability of foods and diets. Nutrient indices are used as functional units (FUs) in life cycle assessment of foods to include nutritional performance in the environmental assessment of a product. Several general and food group-specific nutrient indices exist but many lack validation, particularly when used as FU. In addition, the nutrient selection strategies and reference units for nutrient intake can vary considerably among studies. To vali...
Source: The British Journal of Nutrition - April 12, 2024 Category: Nutrition Authors: Anna K årlund Venla Kytt ä Tiina Pellinen Hanna L Tuomisto Anne-Maria Pajari Marjukka Kolehmainen Merja Saarinen Source Type: research
Validating nutrient selection for product-group-specific nutrient indices for use as functional units in life cycle assessment of foods
Br J Nutr. 2024 Apr 12:1-23. doi: 10.1017/S0007114524000709. Online ahead of print.ABSTRACTThe ability to provide adequate nutrition is considered a key factor in evaluating the sustainability of foods and diets. Nutrient indices are used as functional units (FUs) in life cycle assessment of foods to include nutritional performance in the environmental assessment of a product. Several general and food group-specific nutrient indices exist but many lack validation, particularly when used as FU. In addition, the nutrient selection strategies and reference units for nutrient intake can vary considerably among studies. To vali...
Source: The British Journal of Nutrition - April 12, 2024 Category: Nutrition Authors: Anna K årlund Venla Kytt ä Tiina Pellinen Hanna L Tuomisto Anne-Maria Pajari Marjukka Kolehmainen Merja Saarinen Source Type: research
Validating nutrient selection for product-group-specific nutrient indices for use as functional units in life cycle assessment of foods
Br J Nutr. 2024 Apr 12:1-23. doi: 10.1017/S0007114524000709. Online ahead of print.ABSTRACTThe ability to provide adequate nutrition is considered a key factor in evaluating the sustainability of foods and diets. Nutrient indices are used as functional units (FUs) in life cycle assessment of foods to include nutritional performance in the environmental assessment of a product. Several general and food group-specific nutrient indices exist but many lack validation, particularly when used as FU. In addition, the nutrient selection strategies and reference units for nutrient intake can vary considerably among studies. To vali...
Source: The British Journal of Nutrition - April 12, 2024 Category: Nutrition Authors: Anna K årlund Venla Kytt ä Tiina Pellinen Hanna L Tuomisto Anne-Maria Pajari Marjukka Kolehmainen Merja Saarinen Source Type: research
Biochemical, structural, and computational analyses of two new clinically identified missense mutations of ALDH7A1
We report a biochemical, structural, and computational study of two novel ALDH7A1 missense mutations that were identified in a child with rare recurrent seizures from the third month of life. The mutations affect two residues in the oligomer interfaces of ALDH7A1, Arg134 and Arg441 (Arg162 and Arg469 in the HGVS nomenclature). The corresponding enzyme variants R134S and R441C (p.Arg162Ser and p.Arg469Cys in the HGVS nomenclature) were expressed in Escherichia coli and purified. R134S and R441C have 10,000- and 50-fold lower catalytic efficiency than wild-type ALDH7A1, respectively. Sedimentation velocity analytical ultrace...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: David A Korasick David P Buckley Alessandra Palpacelli Ida Cursio Elisabetta Cesaroni Jianlin Cheng John J Tanner Source Type: research
Biochemical, structural, and computational analyses of two new clinically identified missense mutations of ALDH7A1
We report a biochemical, structural, and computational study of two novel ALDH7A1 missense mutations that were identified in a child with rare recurrent seizures from the third month of life. The mutations affect two residues in the oligomer interfaces of ALDH7A1, Arg134 and Arg441 (Arg162 and Arg469 in the HGVS nomenclature). The corresponding enzyme variants R134S and R441C (p.Arg162Ser and p.Arg469Cys in the HGVS nomenclature) were expressed in Escherichia coli and purified. R134S and R441C have 10,000- and 50-fold lower catalytic efficiency than wild-type ALDH7A1, respectively. Sedimentation velocity analytical ultrace...
Source: Chemico-Biological Interactions - April 11, 2024 Category: Molecular Biology Authors: David A Korasick David P Buckley Alessandra Palpacelli Ida Cursio Elisabetta Cesaroni Jianlin Cheng John J Tanner Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Atherosclerosis - April 7, 2024 Category: Cardiology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Atherosclerosis - April 7, 2024 Category: Cardiology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
pH-dependent regulation of an acidophilic < em > O < /em > -acetylhomoserine sulfhydrylase from < em > Lactobacillus plantarum < /em >
Appl Environ Microbiol. 2024 Apr 3:e0011824. doi: 10.1128/aem.00118-24. Online ahead of print.ABSTRACTBacteria have two routes for the l-methionine biosynthesis. In one route called the direct sulfuration pathway, acetylated l-homoserine is directly converted into l-homocysteine. The reaction using H2S as the second substrate is catalyzed by a pyridoxal 5'-phosphate-dependent enzyme, O-acetylhomoserine sulfhydrylase (OAHS). In the present study, we determined the enzymatic functions and the structures of OAHS from Lactobacillus plantarum (LpOAHS). The LpOAHS enzyme exhibited the highest catalytic activity under the weak ac...
Source: Applied and Environmental Microbiology - April 3, 2024 Category: Microbiology Authors: Yasuyuki Matoba Kosuke Oda Maho Wataeda Hina Kanemori Koichi Matsuo Source Type: research
Boosting the immunogenicity of the CoronaVac SARS-CoV-2 inactivated vaccine with Huoxiang Suling Shuanghua Decoction: a randomized, double-blind, placebo-controlled study
ConclusionOral HSSD boosts the immunogenicity of the CVS vaccine in young and adult individuals. This trial provides clinical reference for evaluation of TCM immunomodulators to improve the immune response to COVID-19 vaccines. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - April 3, 2024 Category: Allergy & Immunology Source Type: research
Biochemical and structural impact of two novel missense mutations in cystathionine beta-synthase gene associated with homocystinuria
In this study, we present the clinical and biochemical characterization of two novel CBS missense mutations that do not respond to pyridoxine treatment, namely c.689T>A (L230Q) and 215A>T (K72I), identified in a Chinese patient. We observed that the disease-associated K72I genetic variant had no apparent effects on the spectroscopic and catalytic properties of the full-length enzyme. In contrast, the L230Q variant expressed in E. coli did not fully retain heme and when compared to the wild-type enzyme, it exhibited more significant impairments in both the canonical cystathionine-synthesis and the alternative H2S-prod...
Source: The Biochemical Journal - April 2, 2024 Category: Biochemistry Authors: Duaa Al-Sadeq Carolina Conter Angelos Thanassoulas Nader Al-Dewik Bared Safieh-Garabedian Luis Alfonso Luis Alfonso Mart ínez-Cruz Gheyath Nasrallah Alessandra Astegno Michail Nomikos Source Type: research
Biochemical and structural impact of two novel missense mutations in cystathionine beta-synthase gene associated with homocystinuria
In this study, we present the clinical and biochemical characterization of two novel CBS missense mutations that do not respond to pyridoxine treatment, namely c.689T>A (L230Q) and 215A>T (K72I), identified in a Chinese patient. We observed that the disease-associated K72I genetic variant had no apparent effects on the spectroscopic and catalytic properties of the full-length enzyme. In contrast, the L230Q variant expressed in E. coli did not fully retain heme and when compared to the wild-type enzyme, it exhibited more significant impairments in both the canonical cystathionine-synthesis and the alternative H2S-prod...
Source: The Biochemical Journal - April 2, 2024 Category: Biochemistry Authors: Duaa Al-Sadeq Carolina Conter Angelos Thanassoulas Nader Al-Dewik Bared Safieh-Garabedian Luis Alfonso Luis Alfonso Mart ínez-Cruz Gheyath Nasrallah Alessandra Astegno Michail Nomikos Source Type: research
