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Correction: Proteasome and thiol involvement in quality control of glycosylphosphatidylinositol anchor addition
Biochem J. 2024 Apr 10;481(7):565-566. doi: 10.1042/BJ3320111_COR.NO ABSTRACTPMID:38597906 | DOI:10.1042/BJ3320111_COR (Source: The Biochemical Journal)
Source: The Biochemical Journal - April 10, 2024 Category: Biochemistry Authors: Barry Wilbourn Darren N Nesbeth Linda J Wainwright Mark C Field Source Type: research

Expression of Concern: Protease-activated receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway
Biochem J. 2024 Apr 10;481(7):567. doi: 10.1042/BCJ20170272_EOC.NO ABSTRACTPMID:38597907 | DOI:10.1042/BCJ20170272_EOC (Source: The Biochemical Journal)
Source: The Biochemical Journal - April 10, 2024 Category: Biochemistry Authors: Chunyang Du Tao Zhang Xia Xiao Yonghong Shi Huijun Duan Yunzhuo Ren Source Type: research

AMP-activated protein kinase can be allosterically activated by ADP but AMP remains the key activating ligand
Biochem J. 2024 Apr 9:BCJ20240082. doi: 10.1042/BCJ20240082. Online ahead of print.ABSTRACTThe AMP-activated protein kinase (AMPK) is a sensor of cellular energy status. When activated by increases in ADP:ATP and/or AMP:ATP ratios (signalling energy deficit), AMPK acts to restore energy balance. Binding of AMP to one or more of three CBS repeats (CBS1, CBS3, CBS4) on the AMPK-γ subunit activates the kinase complex by three complementary mechanisms: (i) promoting α-subunit Thr172 phosphorylation by the upstream kinase LKB1; (ii) protecting against Thr172 dephosphorylation; (iii) allosteric activation. Surprisingly, bindin...
Source: The Biochemical Journal - April 9, 2024 Category: Biochemistry Authors: Simon A Hawley Fiona M Russell D Grahame Hardie Source Type: research

Expression of nNOS alpha and beta isoforms in skeletal muscle of mice
Biochem J. 2024 Apr 9:BCJ20230458. doi: 10.1042/BCJ20230458. Online ahead of print.ABSTRACTKnowledge of the primary structure of neuronal NO synthase (nNOS) in skeletal muscle is still conflicting and needs further clarification. To elucidate the expression patterns of nNOS isoforms at both mRNA and protein level, systematic RT-PCR and epitope mapping by qualitative immunoblot analysis on skeletal muscle of C57/BL6 mice were performed. The ability of the nNOS isoforms to form aggregates was characterized by native low-temperature polyacrylamide electrophoresis (LT-PAGE). The molecular analysis was focused on the rectus fem...
Source: The Biochemical Journal - April 9, 2024 Category: Biochemistry Authors: Oliver Baum Source Type: research

Long-range electron proton coupling in respiratory complex I - insights from molecular simulations of the quinone chamber and antiporter-like subunits
Biochem J. 2024 Apr 10;481(7):499-514. doi: 10.1042/BCJ20240009.ABSTRACTRespiratory complex I is a redox-driven proton pump. Several high-resolution structures of complex I have been determined providing important information about the putative proton transfer paths and conformational transitions that may occur during catalysis. However, how redox energy is coupled to the pumping of protons remains unclear. In this article, we review biochemical, structural and molecular simulation data on complex I and discuss several coupling models, including the key unresolved mechanistic questions. Focusing both on the quinone-reducta...
Source: The Biochemical Journal - April 4, 2024 Category: Biochemistry Authors: Amina Djurabekova Jonathan Lasham Oleksii Zdorevskyi Volker Zickermann Vivek Sharma Source Type: research