Identification of hub genes and key pathways in spinal cord injury via bioinformatics analysis
This study aimed to explore the hub genes and related key pathways in Spinal Cord Injury (SCI) based on the bioinformatics analysis. Two microarray datasets (GSE45006, GSE45550) were obtained from the GEO database and were merged and batch-corrected. The differentially expressed genes (DEGs) in SCI were explored with the Limma, and the weighted gene co-expression network analysis (WGCNA) was conducted to explore the module genes. Functional enrichment analysis and Gene set variation analysis (GSVA) were used to investigate the biological functions and key pathways of the key genes related to SCI. Then the protein-protein i...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hao Cheng Yanben Wang Jun Xuan Source Type: research
Small nucleolar RNA host gene 1 silence inhibits the lipopolysaccharide-induced microglial apoptosis and inflammation
This study was designed to investigate the role of small nucleolar RNA host gene 1 (SNHG1) on the apoptosis and inflammatory response of microglial cell BV-2 and its underlying molecular mechanism. The C5 lamina contusion-induced mouse model of spinal cord injury (SCI) was constructed. Mouse microglia BV2 was stimulated by lipopolysaccharide (LPS) to establish the in vitro model of SCI. The quantitative reverse transcription polymerase chain reaction method was used to quantify RNA expression levels. Enzyme-linked immunosorbent assays were used to quantify concentrations of inflammatory cytokines. Protein levels were asses...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hongtao Shen Jiaao Gu Xing Liu Mowei Song Source Type: research
Identification of hub genes and key pathways in spinal cord injury via bioinformatics analysis
This study aimed to explore the hub genes and related key pathways in Spinal Cord Injury (SCI) based on the bioinformatics analysis. Two microarray datasets (GSE45006, GSE45550) were obtained from the GEO database and were merged and batch-corrected. The differentially expressed genes (DEGs) in SCI were explored with the Limma, and the weighted gene co-expression network analysis (WGCNA) was conducted to explore the module genes. Functional enrichment analysis and Gene set variation analysis (GSVA) were used to investigate the biological functions and key pathways of the key genes related to SCI. Then the protein-protein i...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hao Cheng Yanben Wang Jun Xuan Source Type: research
Small nucleolar RNA host gene 1 silence inhibits the lipopolysaccharide-induced microglial apoptosis and inflammation
This study was designed to investigate the role of small nucleolar RNA host gene 1 (SNHG1) on the apoptosis and inflammatory response of microglial cell BV-2 and its underlying molecular mechanism. The C5 lamina contusion-induced mouse model of spinal cord injury (SCI) was constructed. Mouse microglia BV2 was stimulated by lipopolysaccharide (LPS) to establish the in vitro model of SCI. The quantitative reverse transcription polymerase chain reaction method was used to quantify RNA expression levels. Enzyme-linked immunosorbent assays were used to quantify concentrations of inflammatory cytokines. Protein levels were asses...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hongtao Shen Jiaao Gu Xing Liu Mowei Song Source Type: research
Identification of hub genes and key pathways in spinal cord injury via bioinformatics analysis
This study aimed to explore the hub genes and related key pathways in Spinal Cord Injury (SCI) based on the bioinformatics analysis. Two microarray datasets (GSE45006, GSE45550) were obtained from the GEO database and were merged and batch-corrected. The differentially expressed genes (DEGs) in SCI were explored with the Limma, and the weighted gene co-expression network analysis (WGCNA) was conducted to explore the module genes. Functional enrichment analysis and Gene set variation analysis (GSVA) were used to investigate the biological functions and key pathways of the key genes related to SCI. Then the protein-protein i...
Source: Cellular and Molecular Biology - April 23, 2024 Category: Molecular Biology Authors: Hao Cheng Yanben Wang Jun Xuan Source Type: research
Environmental barriers perceived by the Finnish population with spinal cord injury: a cross-sectional survey
Spinal Cord, Published online: 23 April 2024; doi:10.1038/s41393-024-00990-xEnvironmental barriers perceived by the Finnish population with spinal cord injury: a cross-sectional survey (Source: Spinal Cord)
Source: Spinal Cord - April 23, 2024 Category: Research Authors: Sanna-Mari Saarim äki Paula Reiter ä Anni T äckman Jari Arokoski Aki Vainionp ää Mauri Kallinen Susanna Tallqvist Eerika Koskinen Harri H ämäläinen Anna-Maija Kauppila Heidi Anttila Sinikka Hiekkala Source Type: research
Stepwise combined cell transplantation using mesenchymal stem cells and induced pluripotent stem cell-derived motor neuron progenitor cells in spinal cord injury
CONCLUSIONS: Our findings show that stepwise combined cell therapy can induce alterations in the microenvironment for effective cell therapy in SCI. The in vitro results suggest that co-culturing hMSC and iMN can synergistically promote induction of MN neurite outgrowth.PMID:38650015 | DOI:10.1186/s13287-024-03714-3 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Jang-Woon Kim Juryun Kim Hyunkyung Mo Heeju Han Yeri Alice Rim Ji Hyeon Ju Source Type: research
Ablation of the integrin CD11b mac-1 limits deleterious responses to traumatic spinal cord injury and improves functional recovery in mice
Conclusion: Collectively, our data suggest an important role for CD11b in regulating tissue inflammation and functional outcome following SCI. Thus, the integrin CD11b represents a potential target that may lead to novel therapeutic strategies for SCI.PMID:38645238 | PMC:PMC11030505 | DOI:10.21203/rs.3.rs-4196316/v1 (Source: Cell Research)
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Yun Li Rodney M Ritzel Junyun He Simon Liu Li Zhang Junfang Wu Source Type: research
The beneficial effect of α-lipoic acid on spinal cord injury repair in rats is mediated through inhibition of oxidative stress: A transcriptomic analysis
CONCLUSION: Overall, the findings of this study confirmed the beneficial effects of ALA on recovery in SCI rats through transcriptome sequencing, behavioral, as well histology analyses.PMID:38647358 | DOI:10.1080/10790268.2024.2342058 (Source: Journal of Spinal Cord Medicine)
Source: Journal of Spinal Cord Medicine - April 22, 2024 Category: Orthopaedics Authors: Ming-Ming Bian Yao-Mei Xu Lin Zhang Hua-Zheng Yan Jian-Xiong Gao Gui-Qiang Fu Yang-Yang Wang He-Zuo L ü Source Type: research
Inhibition of UHRF1 Improves Motor Function in Mice with Spinal Cord Injury
The objective of this study was to characterize the differential gene expression and molecular mechanisms in the spinal cord of mice three days after spinal cord injury. By analyzing RNA sequencing data, we identified differentially expressed genes and discovered that the immune system and various metabolic processes play crucial roles in SCI. Additionally, we identified UHRF1 as a key gene that plays a significant role in SCI and found that SCI can be improved by suppressing UHRF1. These findings provide important insights into the molecular mechanisms of SCI and identify potential therapeutic targets that could greatly c...
Source: Cellular and Molecular Neurobiology - April 22, 2024 Category: Cytology Authors: Shuai Cheng Hui Guo Mingyu Bai Yang Cui He Tian Xifan Mei Source Type: research
Perspectives on factors influencing quality of life in persons with long-term spinal cord injury: a qualitative study
Spinal Cord, Published online: 22 April 2024; doi:10.1038/s41393-024-00991-wPerspectives on factors influencing quality of life in persons with long-term spinal cord injury: a qualitative study (Source: Spinal Cord)
Source: Spinal Cord - April 22, 2024 Category: Research Authors: Elliot J. Roth Linda Lovell Alexander Barry Source Type: research
Obesity in wheelchair users with long-standing spinal cord injury: prevalence and associations with time since injury and physical activity
Spinal Cord, Published online: 22 April 2024; doi:10.1038/s41393-024-00995-6Obesity in wheelchair users with long-standing spinal cord injury: prevalence and associations with time since injury and physical activity (Source: Spinal Cord)
Source: Spinal Cord - April 22, 2024 Category: Research Authors: Sonja de Groot Jacinthe J. E. Adriaansen Janneke M. Stolwijk-Sw üste Rutger Osterthun Rita J. G. van den Berg-Emons Marcel W. M. Post Source Type: research
Exosomes derived from vMIP-II-Lamp2b gene-modified M2 cells provide neuroprotection by targeting the injured spinal cord, inhibiting chemokine signals and modulating microglia/macrophage polarization in mice
In this study, using a murine contusive SCI model, we revealed that vMIP-II-Lamp2b-M2-Exo could target the chemokine receptors which highly expressed in the injured spinal cords, inhibit some key chemokine receptor signaling pathways (such as MAPK and Akt), further inhibit proinflammatory factors (such as IL-1β, IL-6, IL-17, IL-18, TNF-α, and iNOS), and promote anti-inflammatory factors (such as IL-4 and Arg1) productions, and the transformation of microglia/macrophages from M1 into M2. Moreover, the improved histological and functional recoveries were also found. Collectively, our results suggest that vMIP-II-Lamp2b-M2-...
Source: Experimental Neurology - April 20, 2024 Category: Neurology Authors: Gui-Qiang Fu Yang-Yang Wang Yao-Mei Xu Ming-Ming Bian Lin Zhang Hua-Zheng Yan Jian-Xiong Gao Jing-Lu Li Yu-Qing Chen Nan Zhang Shu-Qin Ding Rui Wang Jiang-Yan Li Jian-Guo Hu He-Zuo L ü Source Type: research
Pressure ulcers acquired during inpatient rehabilitation after spinal cord injury, characterization and predictors: A 15-years' experience
CONCLUSION: Inpatients can benefit from combined assessments (EMINA + mFIM) and clinical features scarcely addressed in previous studies to prevent PUs.PMID:38640178 | DOI:10.3233/NRE-230234 (Source: NeuroRehabilitation)
Source: NeuroRehabilitation - April 19, 2024 Category: Rehabilitation Authors: Alejandro Garc ía-Rudolph Mark Andrew Wright Emilien Amar Devilleneuve Eulalia Castillo Eloy Opisso Elena Hernandez-Pena Source Type: research
Effect of flipped venous catheter combined with spinal cord electrical stimulation on functional recovery in patients with sciatic nerve injury
CONCLUSIONS: The flipped venous catheter combined with spinal cord electrical stimulation can improve nerve conduction velocity, lower limb motor function, serum BDNF and NGF levels, reduce complications, and help improve the quality of life of sufferers with sciatic nerve injury. Chictr.org.cn ID: ChiCTR2400080984.PMID:38639504 | DOI:10.26355/eurrev_202404_35892 (Source: Pharmacological Reviews)
Source: Pharmacological Reviews - April 19, 2024 Category: Drugs & Pharmacology Authors: S-Y Shi X-H Ou K Jiang Source Type: research
