An improved method for generating human spinal cord neural stem cells
Exp Neurol. 2024 Apr 13:114779. doi: 10.1016/j.expneurol.2024.114779. Online ahead of print.ABSTRACTNeural stem cells have exhibited efficacy in pre-clinical models of spinal cord injury (SCI) and are on a translational path to human testing. We recently reported that neural stem cells must be driven to a spinal cord fate to optimize host axonal regeneration into sites of implantation in the injured spinal cord, where they subsequently form neural relays across the lesion that support significant functional improvement. We also reported methods of deriving and culturing human spinal cord neural stem cells derived from embr...
Source: Experimental Neurology - April 15, 2024 Category: Neurology Authors: Y Li H Kumamaru T Vokes A Tran C Shevinsky L Graham K Archuleta K Limon P Lu A Blesch M H Tuszynski J H Brock Source Type: research
p97 inhibits integrated stress response-induced neuronal apoptosis after subarachnoid hemorrhage in mice by enhancing proteasome function
Exp Neurol. 2024 Apr 10:114778. doi: 10.1016/j.expneurol.2024.114778. Online ahead of print.ABSTRACTNeuronal apoptosis is a common pathological change in early brain injury after subarachnoid hemorrhage (SAH), and it is closely associated with neurological deficits. According to previous research, p97 exhibits a remarkable anti-cardiomyocyte apoptosis effect. p97 is a critical molecule in the growth and development of the nervous system. However, it remains unknown whether p97 can exert an anti-neuronal apoptosis effect in SAH. In the present study, we examined the role of p97 in neuronal apoptosis induced after SAH and in...
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Wenping Cheng Boyang Wei Wenchao Liu Lei Jin Shenquan Guo Mingxiang Ding Yanchao Liu Haiyan Fan Ran Li Xin Zhang Xuying He Xifeng Li Chuanzhi Duan Source Type: research
LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage
CONCLUSION: Exogenous LAMC1 treatment improved neurological deficits after SAH in rats, and attenuated neuronal apoptosis in both in vitro and in vivo SAH models, at least partially through the FAK/PI3K/AKT pathway.PMID:38609046 | DOI:10.1016/j.expneurol.2024.114776 (Source: Experimental Neurology)
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Qiaowei Wu Kaikun Yuan Yanting Yao Jinbiao Yao Jiang Shao Yuxiao Meng Pei Wu Huaizhang Shi Source Type: research
p97 inhibits integrated stress response-induced neuronal apoptosis after subarachnoid hemorrhage in mice by enhancing proteasome function
Exp Neurol. 2024 Apr 10:114778. doi: 10.1016/j.expneurol.2024.114778. Online ahead of print.ABSTRACTNeuronal apoptosis is a common pathological change in early brain injury after subarachnoid hemorrhage (SAH), and it is closely associated with neurological deficits. According to previous research, p97 exhibits a remarkable anti-cardiomyocyte apoptosis effect. p97 is a critical molecule in the growth and development of the nervous system. However, it remains unknown whether p97 can exert an anti-neuronal apoptosis effect in SAH. In the present study, we examined the role of p97 in neuronal apoptosis induced after SAH and in...
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Wenping Cheng Boyang Wei Wenchao Liu Lei Jin Shenquan Guo Mingxiang Ding Yanchao Liu Haiyan Fan Ran Li Xin Zhang Xuying He Xifeng Li Chuanzhi Duan Source Type: research
LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage
CONCLUSION: Exogenous LAMC1 treatment improved neurological deficits after SAH in rats, and attenuated neuronal apoptosis in both in vitro and in vivo SAH models, at least partially through the FAK/PI3K/AKT pathway.PMID:38609046 | DOI:10.1016/j.expneurol.2024.114776 (Source: Experimental Neurology)
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Qiaowei Wu Kaikun Yuan Yanting Yao Jinbiao Yao Jiang Shao Yuxiao Meng Pei Wu Huaizhang Shi Source Type: research
p97 inhibits integrated stress response-induced neuronal apoptosis after subarachnoid hemorrhage in mice by enhancing proteasome function
Exp Neurol. 2024 Apr 10:114778. doi: 10.1016/j.expneurol.2024.114778. Online ahead of print.ABSTRACTNeuronal apoptosis is a common pathological change in early brain injury after subarachnoid hemorrhage (SAH), and it is closely associated with neurological deficits. According to previous research, p97 exhibits a remarkable anti-cardiomyocyte apoptosis effect. p97 is a critical molecule in the growth and development of the nervous system. However, it remains unknown whether p97 can exert an anti-neuronal apoptosis effect in SAH. In the present study, we examined the role of p97 in neuronal apoptosis induced after SAH and in...
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Wenping Cheng Boyang Wei Wenchao Liu Lei Jin Shenquan Guo Mingxiang Ding Yanchao Liu Haiyan Fan Ran Li Xin Zhang Xuying He Xifeng Li Chuanzhi Duan Source Type: research
LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage
CONCLUSION: Exogenous LAMC1 treatment improved neurological deficits after SAH in rats, and attenuated neuronal apoptosis in both in vitro and in vivo SAH models, at least partially through the FAK/PI3K/AKT pathway.PMID:38609046 | DOI:10.1016/j.expneurol.2024.114776 (Source: Experimental Neurology)
Source: Experimental Neurology - April 12, 2024 Category: Neurology Authors: Qiaowei Wu Kaikun Yuan Yanting Yao Jinbiao Yao Jiang Shao Yuxiao Meng Pei Wu Huaizhang Shi Source Type: research
Mice harboring the T316N variant in the GABA < sub > A < /sub > R γ < sub > 2 < /sub > subunit exhibit sleep-related hypermotor epilepsy phenotypes and hypersynchronization in the thalamocortical pathway
CONCLUSION: We generated a new SHE mouse model and provided in vivo evidence that rare variants of GABRG2 contribute to seizure attacks during sleep in SHE.PMID:38604438 | DOI:10.1016/j.expneurol.2024.114775 (Source: Experimental Neurology)
Source: Experimental Neurology - April 11, 2024 Category: Neurology Authors: Yong-Li Jiang Liang Xia Jing-Jing Zhao Hui-Min Zhou Dan Mi Xuan Wang Yuan-Yuan Wang Chang-Geng Song Wen Jiang Source Type: research
Mice harboring the T316N variant in the GABA < sub > A < /sub > R γ < sub > 2 < /sub > subunit exhibit sleep-related hypermotor epilepsy phenotypes and hypersynchronization in the thalamocortical pathway
CONCLUSION: We generated a new SHE mouse model and provided in vivo evidence that rare variants of GABRG2 contribute to seizure attacks during sleep in SHE.PMID:38604438 | DOI:10.1016/j.expneurol.2024.114775 (Source: Experimental Neurology)
Source: Experimental Neurology - April 11, 2024 Category: Neurology Authors: Yong-Li Jiang Liang Xia Jing-Jing Zhao Hui-Min Zhou Dan Mi Xuan Wang Yuan-Yuan Wang Chang-Geng Song Wen Jiang Source Type: research
Type selective ablation of postnatal slow and fast fatigue-resistant motor neurons in mice induces late onset kinetic and postural tremor following fiber-type transition and myopathy
Exp Neurol. 2024 Apr 8:114772. doi: 10.1016/j.expneurol.2024.114772. Online ahead of print.ABSTRACTAnimals on Earth need to hold postures and execute a series of movements under gravity and atmospheric pressure. VAChT-Cre is a transgenic Cre driver mouse line that expresses Cre recombinase selectively in motor neurons of S-type (slow-twitch fatigue-resistant) and FR-type (fast-twitch fatigue-resistant). Sequential motor unit recruitment is a fundamental principle for fine and smooth locomotion; smaller-diameter motor neurons (S-type, FR-type) first contract low-intensity oxidative type I and type IIa muscle fibers, and the...
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Hidemi Misawa Kai Kamishima Tenkei Koyama Lisa Ohgaki Yuta Morisaki Tomoyuki Yamanaka Shigeyoshi Itohara Shoko Sawano Wataru Mizunoya Naomichi Ogihara Source Type: research
Rejuvenating fecal microbiota transplant enhances peripheral nerve repair in aged mice by modulating endoneurial inflammation
This study thus aimed to evaluate the pro-regenerative effects of a rejuvenating fecal microbiota transplant (FMT) in a preclinical PNI model using aged mice. Aged C57BL/6 mice underwent bilateral crush injuries to their sciatic nerves. Subsequently, they either received FMT from young donors at three and four days after the injury or retained their aged gut microbiota. We analyzed gut microbiome composition and SCFA concentrations in fecal samples. The integrity of the ileac mucosal barrier was assessed by immunofluorescence staining of Claudin-1. Flow cytometry was utilized to examine immune cells and cytokine production...
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Martin K R Sva čina Tong Gao Alina Sprenger-Sva čina Jianxin Lin Bhanu P Ganesh Juneyoung Lee Louise D McCullough Kazim A Sheikh Gang Zhang Source Type: research
Decreased connexin 40 expression of the sinoatrial node mediates ischemic stroke-induced arrhythmia in mice
CONCLUSIONS: In conclusion, decreased connexin 40 expression in the sinoatrial node contributed to the ischemic stroke-induced cardiac arrhythmia. Therefore, enhancing connexin 40 expression holds promise as a potential therapeutic approach for ischemic stroke-induced arrhythmia.PMID:38599368 | DOI:10.1016/j.expneurol.2024.114773 (Source: Experimental Neurology)
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Zhi-Jun Yao Yan-Pin Jiang Dan Yuan Pu Hong Meng-Jiao He Feng-Xian Li Shi-Yuan Xu Hong-Bin Lin Hong-Fei Zhang Source Type: research
Type selective ablation of postnatal slow and fast fatigue-resistant motor neurons in mice induces late onset kinetic and postural tremor following fiber-type transition and myopathy
Exp Neurol. 2024 Apr 8:114772. doi: 10.1016/j.expneurol.2024.114772. Online ahead of print.ABSTRACTAnimals on Earth need to hold postures and execute a series of movements under gravity and atmospheric pressure. VAChT-Cre is a transgenic Cre driver mouse line that expresses Cre recombinase selectively in motor neurons of S-type (slow-twitch fatigue-resistant) and FR-type (fast-twitch fatigue-resistant). Sequential motor unit recruitment is a fundamental principle for fine and smooth locomotion; smaller-diameter motor neurons (S-type, FR-type) first contract low-intensity oxidative type I and type IIa muscle fibers, and the...
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Hidemi Misawa Kai Kamishima Tenkei Koyama Lisa Ohgaki Yuta Morisaki Tomoyuki Yamanaka Shigeyoshi Itohara Shoko Sawano Wataru Mizunoya Naomichi Ogihara Source Type: research
Rejuvenating fecal microbiota transplant enhances peripheral nerve repair in aged mice by modulating endoneurial inflammation
This study thus aimed to evaluate the pro-regenerative effects of a rejuvenating fecal microbiota transplant (FMT) in a preclinical PNI model using aged mice. Aged C57BL/6 mice underwent bilateral crush injuries to their sciatic nerves. Subsequently, they either received FMT from young donors at three and four days after the injury or retained their aged gut microbiota. We analyzed gut microbiome composition and SCFA concentrations in fecal samples. The integrity of the ileac mucosal barrier was assessed by immunofluorescence staining of Claudin-1. Flow cytometry was utilized to examine immune cells and cytokine production...
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Martin K R Sva čina Tong Gao Alina Sprenger-Sva čina Jianxin Lin Bhanu P Ganesh Juneyoung Lee Louise D McCullough Kazim A Sheikh Gang Zhang Source Type: research
Decreased connexin 40 expression of the sinoatrial node mediates ischemic stroke-induced arrhythmia in mice
CONCLUSIONS: In conclusion, decreased connexin 40 expression in the sinoatrial node contributed to the ischemic stroke-induced cardiac arrhythmia. Therefore, enhancing connexin 40 expression holds promise as a potential therapeutic approach for ischemic stroke-induced arrhythmia.PMID:38599368 | DOI:10.1016/j.expneurol.2024.114773 (Source: Experimental Neurology)
Source: Experimental Neurology - April 10, 2024 Category: Neurology Authors: Zhi-Jun Yao Yan-Pin Jiang Dan Yuan Pu Hong Meng-Jiao He Feng-Xian Li Shi-Yuan Xu Hong-Bin Lin Hong-Fei Zhang Source Type: research
