Neuroligin1 in excitatory synapses contributes to long-term cognitive impairments after repeated neonatal sevoflurane exposures
CONCLUSION: We have demonstrated that repeated neonatal sevoflurane exposures decreased inhibitory synaptic inputs (labelled by vGAT) around neurons, which may influence the upregulation of NL1 in hippocampal excitatory synapses and enhanced NL1/PSD95 interaction, ultimately leading to long-term cognitive impairments in adolescent rats. Injecting NL1--AAV reversed this damage. These results suggested that NL1 in excitatory synapses contributes to long-term cognitive impairments after repeated neonatal sevoflurane exposures.PMID:38493982 | DOI:10.1016/j.expneurol.2024.114755 (Source: Experimental Neurology)
Source: Experimental Neurology - March 17, 2024 Category: Neurology Authors: Hui Zhang Yingqiao Niu Peng Yuan Wenbo Liu Wei Zhu Jie Sun Source Type: research

Multi-session transcutaneous spinal cord stimulation prevents chloride homeostasis imbalance and the development of hyperreflexia after spinal cord injury in rat
Exp Neurol. 2024 Mar 15:114754. doi: 10.1016/j.expneurol.2024.114754. Online ahead of print.ABSTRACTSpasticity is a complex and multidimensional disorder that impacts nearly 75% of individuals with spinal cord injury (SCI) and currently lacks adequate treatment options. This sensorimotor condition is burdensome as hyperexcitability of reflex pathways result in exacerbated reflex responses, co-contractions of antagonistic muscles, and involuntary movements. Transcutaneous spinal cord stimulation (tSCS) has become a popular tool in the human SCI research field. The likeliness for this intervention to be successful as a nonin...
Source: Experimental Neurology - March 17, 2024 Category: Neurology Authors: Dillon C Malloy Marie-Pascale C ôté Source Type: research

MircoRNA-25-3p in skin precursor cell-induced Schwann cell-derived extracellular vesicles promotes axon regeneration by targeting Tgif1
This study elucidated the microRNA (miRNA) expression profiles of two types of EVs that promote nerve regeneration. The functions of these miRNAs were screened in vitro. Among the 12 overlapping miRNAs, miR-25-3p was selected for further analysis as it markedly promoted axon regeneration both in vivo and in vitro. Furthermore, knockdown experiments confirmed that PTEN and Klf4, which are the major inhibitors of axon regeneration, were the direct targets of miR-25-3p in dorsal root ganglion (DRG) neurons. The utilization of luciferase reporter assays and functional tests provided evidence that miR-25-3p enhances axon regene...
Source: Experimental Neurology - March 16, 2024 Category: Neurology Authors: Meng Cong Jiyu Li Lijuan Wang Chang Liu Mengru Zheng Qiang Zhou Mingzhi Du Xinli Ye Min Feng Yujiao Ye Shuyu Zhang Wenqing Xu Yi Lu Cheng Wang Yingjie Xia Huimin Xie Yide Zhang Qianru He Leilei Gong Yun Gu Hualin Sun Qi Zhang Jian Zhao Fei Ding Xiaosong G Source Type: research

Pharmacological and stem cell therapy of stroke in animal models: Do they accurately reflect the response of humans?
Exp Neurol. 2024 Mar 14;376:114753. doi: 10.1016/j.expneurol.2024.114753. Online ahead of print.ABSTRACTCerebrovascular diseases are the second leading cause of death worldwide. Despite significant research investment, the only available therapeutic options are mechanical thrombectomy and tissue plasminogen activator thrombolysis. None of the more than a thousand drugs tested on animal models have proven successful in human clinical trials. Several factors contribute to this poor translation of data from stroke-related animal models to human stroke patients. Firstly, our understanding of the molecular and cellular processe...
Source: Experimental Neurology - March 15, 2024 Category: Neurology Authors: Mihai Ruscu Daniela Glavan Roxana Surugiu Thorsten R Doeppner Dirk M Hermann Andrei Gresita Bogdan Capitanescu Aurel Popa-Wagner Source Type: research

Pharmacological and stem cell therapy of stroke in animal models: Do they accurately reflect the response of humans?
Exp Neurol. 2024 Mar 13:114753. doi: 10.1016/j.expneurol.2024.114753. Online ahead of print.ABSTRACTCerebrovascular diseases are the second leading cause of death worldwide. Despite significant research investment, the only available therapeutic options are mechanical thrombectomy and tissue plasminogen activator thrombolysis. None of the more than a thousand drugs tested on animal models have proven successful in human clinical trials. Several factors contribute to this poor translation of data from stroke-related animal models to human stroke patients. Firstly, our understanding of the molecular and cellular processes in...
Source: Experimental Neurology - March 15, 2024 Category: Neurology Authors: Mihai Ruscu Daniela Glavan Roxana Surugiu Thorsten R Doeppner Dirk M Hermann Andrei Gresita Bogdan Capitanescu Aurel Popa-Wagner Source Type: research

MARK1 regulates dendritic spine morphogenesis and cognitive functions in vivo
Exp Neurol. 2024 Mar 12:114752. doi: 10.1016/j.expneurol.2024.114752. Online ahead of print.ABSTRACTDendritic spines play a pivotal role in synaptic communication and are crucial for learning and memory processes. Abnormalities in spine morphology and plasticity are observed in neurodevelopmental and neuropsychiatric disorders, yet the underlying signaling mechanisms remain poorly understood. The microtubule affinity regulating kinase 1 (MARK1) has been implicated in neurodevelopmental disorders, and the MARK1 gene shows accelerated evolution in the human lineage suggesting a role in cognition. However, the in vivo role of...
Source: Experimental Neurology - March 14, 2024 Category: Neurology Authors: Emily C Kelly-Castro Rebecca Shear Ankitha H Dindigal Maitreyee Bhagwat Huaye Zhang Source Type: research

The role of dorsolateral striatum in the effects of deep cerebellar stimulation-mediated motor recovery following ischemic stroke in rodents
In this study, we compared the motor rehabilitative effects of deep cerebellar stimulation in two rodent models of cerebral ischemia: a) cortical ischemia; and b) combined striatal and cortical ischemia. All animals underwent the same procedures, including implantation of the electrodes and tethered connections for stimulation. Both experimental groups received four weeks of continuous lateral cerebellar nucleus (LCN) DBS and each was paired with a no stimulation, sham, group. Fine motor function was indexed using the pasta matrix task. Brain tissue was harvested for histology and immunohistochemical analyses. In the corti...
Source: Experimental Neurology - March 14, 2024 Category: Neurology Authors: Hugh H Chan Nicole D Mathews Hemen Khanna Nymisha Mandava Olivia Hogue Andre G Machado Kenneth B Baker Source Type: research

MARK1 regulates dendritic spine morphogenesis and cognitive functions in vivo
Exp Neurol. 2024 Mar 12:114752. doi: 10.1016/j.expneurol.2024.114752. Online ahead of print.ABSTRACTDendritic spines play a pivotal role in synaptic communication and are crucial for learning and memory processes. Abnormalities in spine morphology and plasticity are observed in neurodevelopmental and neuropsychiatric disorders, yet the underlying signaling mechanisms remain poorly understood. The microtubule affinity regulating kinase 1 (MARK1) has been implicated in neurodevelopmental disorders, and the MARK1 gene shows accelerated evolution in the human lineage suggesting a role in cognition. However, the in vivo role of...
Source: Experimental Neurology - March 14, 2024 Category: Neurology Authors: Emily C Kelly-Castro Rebecca Shear Ankitha H Dindigal Maitreyee Bhagwat Huaye Zhang Source Type: research

The role of dorsolateral striatum in the effects of deep cerebellar stimulation-mediated motor recovery following ischemic stroke in rodents
In this study, we compared the motor rehabilitative effects of deep cerebellar stimulation in two rodent models of cerebral ischemia: a) cortical ischemia; and b) combined striatal and cortical ischemia. All animals underwent the same procedures, including implantation of the electrodes and tethered connections for stimulation. Both experimental groups received four weeks of continuous lateral cerebellar nucleus (LCN) DBS and each was paired with a no stimulation, sham, group. Fine motor function was indexed using the pasta matrix task. Brain tissue was harvested for histology and immunohistochemical analyses. In the corti...
Source: Experimental Neurology - March 14, 2024 Category: Neurology Authors: Hugh H Chan Nicole D Mathews Hemen Khanna Nymisha Mandava Olivia Hogue Andre G Machado Kenneth B Baker Source Type: research

Characterization of the intrahippocampal kainic acid model in female mice with a special focus on seizure suppression by antiseizure medications
Exp Neurol. 2024 Mar 11;376:114749. doi: 10.1016/j.expneurol.2024.114749. Online ahead of print.ABSTRACTDespite special challenges in the medical treatment of women with epilepsy, in particular preclinical animal studies were focused on males for decades and females have only recently moved into the focus of scientific interest. The intrahippocampal kainic acid (IHKA) mouse model of temporal lobe epilepsy (TLE) is one of the most studied models in males reproducing electroencephalographic (EEG) and histopathological features of human TLE. Hippocampal paroxysmal discharges (HPDs) were described as drug resistant focal seizu...
Source: Experimental Neurology - March 11, 2024 Category: Neurology Authors: Melanie Widmann Andreas Lieb Barbara Fogli Angela Steck Anna Mutti Christoph Schwarzer Source Type: research

Characterization of the intrahippocampal kainic acid model in female mice with a special focus on seizure suppression by antiseizure medications
Exp Neurol. 2024 Mar 9:114749. doi: 10.1016/j.expneurol.2024.114749. Online ahead of print.ABSTRACTDespite special challenges in the medical treatment of women with epilepsy, in particular preclinical animal studies were focused on males for decades and females have only recently moved into the focus of scientific interest. The intrahippocampal kainic acid (IHKA) mouse model of temporal lobe epilepsy (TLE) is one of the most studied models in males reproducing electroencephalographic (EEG) and histopathological features of human TLE. Hippocampal paroxysmal discharges (HPDs) were described as drug resistant focal seizures i...
Source: Experimental Neurology - March 11, 2024 Category: Neurology Authors: Melanie Widmann Andreas Lieb Barbara Fogli Angela Steck Anna Mutti Christoph Schwarzer Source Type: research

Characterization of the intrahippocampal kainic acid model in female mice with a special focus on seizure suppression by antiseizure medications
Exp Neurol. 2024 Mar 9:114749. doi: 10.1016/j.expneurol.2024.114749. Online ahead of print.ABSTRACTDespite special challenges in the medical treatment of women with epilepsy, in particular preclinical animal studies were focused on males for decades and females have only recently moved into the focus of scientific interest. The intrahippocampal kainic acid (IHKA) mouse model of temporal lobe epilepsy (TLE) is one of the most studied models in males reproducing electroencephalographic (EEG) and histopathological features of human TLE. Hippocampal paroxysmal discharges (HPDs) were described as drug resistant focal seizures i...
Source: Experimental Neurology - March 11, 2024 Category: Neurology Authors: Melanie Widmann Andreas Lieb Barbara Fogli Angela Steck Anna Mutti Christoph Schwarzer Source Type: research

Floralozone regulates MiR-7a-5p expression through AMPK α2 activation to improve cognitive dysfunction in vascular dementia
CONCLUSION: Floralozone could regulate miR-7a-5p expression level by activating the protein expression of AMPKα2, control the protein expression of TRPM2 and NR2B, improve the morphology and structure of hippocampus neurons, reduce the apoptosis of hippocampus neurons, promote neurogenesis and improve the cognitive dysfunction.PMID:38458310 | DOI:10.1016/j.expneurol.2024.114748 (Source: Experimental Neurology)
Source: Experimental Neurology - March 8, 2024 Category: Neurology Authors: Yu-Ting Song Shan-Shan Li Chun-Yan Chao None Shuang-Guo Gui-Zi Chen Shuang-Xi Wang Ming-Xiang Zhang Ya-Ling Yin Peng Li Source Type: research

Floralozone regulates MiR-7a-5p expression through AMPK α2 activation to improve cognitive dysfunction in vascular dementia
CONCLUSION: Floralozone could regulate miR-7a-5p expression level by activating the protein expression of AMPKα2, control the protein expression of TRPM2 and NR2B, improve the morphology and structure of hippocampus neurons, reduce the apoptosis of hippocampus neurons, promote neurogenesis and improve the cognitive dysfunction.PMID:38458310 | DOI:10.1016/j.expneurol.2024.114748 (Source: Experimental Neurology)
Source: Experimental Neurology - March 8, 2024 Category: Neurology Authors: Yu-Ting Song Shan-Shan Li Chun-Yan Chao None Shuang-Guo Gui-Zi Chen Shuang-Xi Wang Ming-Xiang Zhang Ya-Ling Yin Peng Li Source Type: research

Floralozone regulates MiR-7a-5p expression through AMPK α2 activation to improve cognitive dysfunction in vascular dementia
CONCLUSION: Floralozone could regulate miR-7a-5p expression level by activating the protein expression of AMPKα2, control the protein expression of TRPM2 and NR2B, improve the morphology and structure of hippocampus neurons, reduce the apoptosis of hippocampus neurons, promote neurogenesis and improve the cognitive dysfunction.PMID:38458310 | DOI:10.1016/j.expneurol.2024.114748 (Source: Experimental Neurology)
Source: Experimental Neurology - March 8, 2024 Category: Neurology Authors: Yu-Ting Song Shan-Shan Li Chun-Yan Chao None Shuang-Guo Gui-Zi Chen Shuang-Xi Wang Ming-Xiang Zhang Ya-Ling Yin Peng Li Source Type: research