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Infectious Disease: Bird Flu

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Total 56 results found since Jan 2013.

The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication
In this study, we found that the expression and transcription levels of RPL18 was reduced early in NDV infection but increased later in NDV infection. In addition, the presence of cytoplasmic NDV matrix (M) protein was responsible for the increased expression of RPL18 in both virus-infected cells and plasmid-transfected cells. Moreover, cytoplasmic M protein increased RPL18 expression in a dose-dependent manner, even though they did not interact with each other. Furthermore, siRNA-mediated knockdown of RPL18 or overexpression of RPL18 dramatically reduced or enhanced NDV replication by decreasing or increasing viral protei...
Source: Avian Pathology - December 3, 2021 Category: Pathology Authors: Zhiqiang Duan Hong Tang Yanbi Wang Caiqin Zhao Lei Zhou Yifan Han Source Type: research

Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/ β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells
This study aimed to explore the mutual regulation between chicken telomerase reverse transcriptase (chTERT) and the Wnt/β-catenin signalling pathway and its effects on cell growth and avian leukosis virus subgroup J (ALV-J) replication in LMH cells. First, LMH cells stably overexpressing the chTERT gene (LMH-chTERT cells) and corresponding control cells (LMH-NC cells) were successfully constructed with a lentiviral vector expression system. The results showed that chTERT upregulated the expression of β-catenin, Cyclin D1, TCF4 and c-Myc. chTERT expression level and telomerase activity were increased when cells were treat...
Source: Cell Research - August 20, 2021 Category: Cytology Authors: Yong Xiang Yun Yu Qingbo Li Zeng Jiang Jinqun Li Canxin Liang Jian Chen Yu Li Xiaoyan Chen Weisheng Cao Source Type: research

Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response
This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN- β. A small...
Source: Veterinary Research - December 9, 2020 Category: Veterinary Research Source Type: research

Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light.
Abstract As the circadian pacemaker of birds, the retina possesses the ability to receive light information, generate circadian oscillation, and secrete melatonin. Previous studies have confirmed that monochromatic green light can accelerate the circadian rhythmic expression of clock genes in the chick retina, thereby increasing cAanat mRNA level and melatonin secretion. However, as the core components of the transcriptional-translational negative feedback loop, the role that cBmal1 and cClock plays in the regulation of the retinal molecular clock system and melatonin secretion under monochromatic green light is u...
Source: Chronobiology International - October 28, 2020 Category: Biology Authors: Bian J, Wang Z, Dong Y, Cao J, Chen Y Tags: Chronobiol Int Source Type: research

NLRC5 Serves as a Pro-viral Factor During Influenza Virus Infection in Chicken Macrophages
In this study, we found that NLRC5 is activated by a range of low and highly pathogenic AIVs in primary chicken lung cells and a chicken macrophage cell line. Further, siRNA mediated NLRC5 knockdown in chicken macrophages resulted in a significant reduction in AIV replication which was associated with the upregulation of genes associated with activated NFκB signaling pathway. The knockdown of NLRC5 enhanced the expression of genes known to be associated with viral defense and decreased innate cytokine gene expression following AIV infection. Overall, our investigation strongly suggests that NLRC5 is a pro-viral factor dur...
Source: Frontiers in cellular and infection microbiology - May 18, 2020 Category: Microbiology Source Type: research

Molecular identification of duck DDX3X and its potential role in response to Tembusu virus.
This study will contribute to a better understanding of duDDX3X in the innate immune system of ducks and lay a solid foundation for further studies of duDDX3X in antiviral immunity. PMID: 31899305 [PubMed - as supplied by publisher]
Source: Developmental and Comparative Immunology - December 29, 2019 Category: Allergy & Immunology Authors: Li N, Jiang S, Zhao J, Yang Y, Deng Kai, Wei L, Cai Y, Li B, Liu S Tags: Dev Comp Immunol Source Type: research

Gga-miR-19b-3p Inhibits Newcastle Disease Virus Replication by Suppressing Inflammatory Response via Targeting RNF11 and ZMYND11
Newcastle disease (ND), an acute and highly contagious avian disease caused by virulent Newcastle disease virus (NDV), often results in severe economic losses worldwide every year. Although it is clear that microRNAs (miRNAs) are implicated in modulating innate immune response to invading microbial pathogens, their role in host defense against NDV infection remains largely unknown. Our prior study indicates that gga-miR-19b-3p is up-regulated in NDV-infected DF-1 cells (a chicken embryo fibroblast cell line) and functions to suppress NDV replication. Here we report that overexpression of gga-miR-19b-3p promoted the product...
Source: Frontiers in Microbiology - August 26, 2019 Category: Microbiology Source Type: research

Tangshen Formula Alleviates Hepatic Steatosis by Inducing Autophagy Through the AMPK/SIRT1 Pathway
Conclusion In conclusion, the present study demonstrated that autophagy was involved in relieving the effects of TSF against NAFLD, which were mediated by the AMPK/SIRT1 pathway (Figure 7D). These findings may improve our current understanding of the role of TSF in treating hepatic steatosis and provide an experimental basis for the clinical application of TSF in NAFLD and its related metabolic syndrome. Ethics Statement This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Ethics Co...
Source: Frontiers in Physiology - April 25, 2019 Category: Physiology Source Type: research

Gene Therapy Leaves a Vicious Cycle
Reena Goswami1, Gayatri Subramanian2, Liliya Silayeva1, Isabelle Newkirk1, Deborah Doctor1, Karan Chawla2, Saurabh Chattopadhyay2, Dhyan Chandra3, Nageswararao Chilukuri1 and Venkaiah Betapudi1,4* 1Neuroscience Branch, Research Division, United States Army Medical Research Institute of Chemical Defense, Aberdeen, MD, United States 2Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, United States 3Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States 4Department of Physiology and Biophysics, Case Western Reserve University, Clev...
Source: Frontiers in Oncology - April 23, 2019 Category: Cancer & Oncology Source Type: research

Why Do Birds Flock? A Role for Opioids in the Reinforcement of Gregarious Social Interactions
Conclusion We propose that studies of songbirds reveal a novel network model for the integration of positive and negative reinforcement processes in non-sexual affiliative social behavior. Most studies on affiliative behavior focus on the positive affective state induced by social contact that rewards individuals interacting together. However, this review highlights that in social animals, affiliative contact is also reinforced because it reduces a negative affective state caused by social exclusion or isolation, thus creating a complementary system (i.e., positive reinforcement from affiliative interactions and negative ...
Source: Frontiers in Physiology - April 11, 2019 Category: Physiology Source Type: research

To accelerate the Zika beat: candidate design for RNA interference-based therapy.
Abstract Zika virus infection is associated with the development of severe neurological disorders in adults and newborns. Although at the moment Zika virus outbreak is not threatening to become again an emergency, infection cases are still being sporadically reported and there is still no effective therapy available. A possible treatment to suppress Zika replication is represented by short interfering RNAs (siRNAs), since they have been successfully used even against Ebola, H5N1 and SARS viruses and clinical trials of siRNA-based drugs are ongoing. In order to speed up the time consuming experimental validation of...
Source: Virus Research - July 18, 2018 Category: Virology Authors: Giulietti M, Righetti A, Cianfruglia L, Šabanović B, Armeni T, Principato G, Piva F Tags: Virus Res Source Type: research

The PA-interacting host protein nucleolin acts as an antiviral factor during highly pathogenic H5N1 avian influenza virus infection
In this study, we further explored the role of NCL during highly pathogenic H5N1 avian influenza virus infection. We found that depletion of endogenous NCL in mammalian cells by siRNA targeting during H5N1 infection resulted in significantly increased viral polymerase activity, elevated viral mRNA, cRNA and vRNA synthesis, accelerated viral replication, and enhanced apoptosis and necrosis. Moreover, siRNA silencing of NCL significantly exacerbated the inflammatory response, resulting in increased secretion of IL-6, TNF- α, TNF-β, CCL-4, CCL-8, IFN-α, IFN-β and IFN-γ. Conversely, overexpression of NCL significantly dec...
Source: Archives of Virology - July 5, 2018 Category: Virology Source Type: research

Non-structural protein 1 from avian influenza virus H9N2 is an efficient RNA silencing suppressor with characteristics that differ from those of Tomato bushy stunt virus p19
In this study, we showed that the NS1 protein from avian influenza virus (AIV) H9N2 suppressed systemic RNA silencing induced by sense RNA or dsRNA. It resulted in more severePotato virus X symptom, but could not reverse established systemic green fluorescent protein silencing inNicotiana benthamiana. In addition, its systemic silencing suppression activity was much weaker than that of p19. The local silencing suppression activity of AIV H9N2 NS1 was most powerful at 7  dpi and was even stronger than that of p19. And the inhibition ability to RNA silencing of NS1 is stronger than that of p19 in human cells. Collectively, ...
Source: Virus Genes - February 26, 2018 Category: Genetics & Stem Cells Source Type: research

Downregulation of 14-3-3 β inhibits proliferation and migration in osteosarcoma cells.
Downregulation of 14-3-3β inhibits proliferation and migration in osteosarcoma cells. Mol Med Rep. 2017 Nov 22;: Authors: Wu Q, Zhu J, Liu F, Liu J, Li M Abstract The 14-3-3 protein isoform β (14‑3‑3β), which is an intracellular adaptor protein that exists in all eukaryotic organisms, is highly expressed in many cancer tissues, including glioma, lung carcinoma and breast cancer. However, 14‑3‑3β expression and function in osteosarcoma progression remain unknown. In the present study, the endogenous expression of 14‑3‑3β was assessed in osteosarcoma samples and the effect of 14‑3‑3Î...
Source: Molecular Medicine Reports - December 6, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research