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Infectious Disease: Bird Flu

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Total 56 results found since Jan 2013.

Expression of a single siRNA against a conserved region of NP gene strongly inhibits in vitro replication of different Influenza A virus strains of avian and swine origin.
In conclusion, these findings reveal new siRNA sequences able to inhibit Influenza A virus replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection both in humans and animals. PMID: 25986248 [PubMed - as supplied by publisher]
Source: Antiviral Research - May 16, 2015 Category: Virology Authors: Stoppani E, Bassi I, Dotti S, Lizier M, Ferrari M, Lucchini F Tags: Antiviral Res Source Type: research

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth
We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth.
Source: Archives of Virology - April 10, 2015 Category: Virology Source Type: research

Synergistic inhibition of avian leukosis virus subgroup J replication by miRNA-embedded siRNA interference of double-target
Conclusions: These data demonstrated that the miRNA-embedded siRNA interference is an efficient method for inhibition of ALV-J replication, especially double targets.
Source: Epidemiologic Perspectives and Innovations - March 21, 2015 Category: Epidemiology Authors: Rongrong WeiXiaoqian MaGuihua WangHuijun GuoJianzhu LiuLingxiao FanZiqiang Cheng Source Type: research

Avian reovirus S1133-induced apoptosis is associated with Bip/GRP79-mediated Bim translocation to the endoplasmic reticulum
In this study the mechanism of avian reovirus (ARV) S1133-induced pathogenesis was investigated, with a focus on the contribution of ER stress to apoptosis. Our results showed that upregulation of the ER stress response protein, as well as caspase-3 activation, occurred in ARV S1133-infected cultured cells and in SPF White Leghorn chicks organs. Upon infection, Bim was translocated specifically to the ER, but not mitochondria, in the middle to late infectious stages. In addition, ARV S1133 induced JNK phosphorylation and promoted JNK–Bim complex formation, which correlated with the Bim translocation and apoptosis inducti...
Source: Apoptosis - January 9, 2015 Category: Molecular Biology Source Type: research

Effects of interferon-γ knockdown on vaccine-induced immunity against Marek's disease in chickens.
Abstract Interferon (IFN)-γ has been shown to be associated with immunity to Marek's disease virus (MDV). The overall objective of this study was to investigate the causal relationship between IFN-γ and vaccine-conferred immunity against MDV in chickens. To this end, 3 small interfering RNAs (siRNAs) targeting chicken IFN-γ, which had previously been shown to reduce IFN-γ expression in vitro, and a control siRNA were selected to generate recombinant avian adeno-associated virus (rAAAV) expressing short-hairpin small interfering RNAs (shRNAs). An MDV challenge trial was then conducted: chickens were vaccinated ...
Source: Canadian Journal of Veterinary Research - January 1, 2015 Category: Veterinary Research Authors: Haq K, Wootton SK, Barjesteh N, Golovan S, Bendall A, Sharif S Tags: Can J Vet Res Source Type: research

Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
Conclusions: A novel combination of antiviral siRNAs expressed in chicken cells and chicken antibody single-chain variable fragments (scFvs) secreted from the cells has a synergistic inhibitory effect on the avian influenza viral proliferation in vitro. Intracellular application of scFvs and anti-viral siRNA may provide a new approach to influenza prevention and treatment.
Source: Virology Journal - November 29, 2014 Category: Virology Authors: Shuang WangPeng ZhangFei HeJi-Gui WangJia-Zeng SunZhi-Li LiBao YiJi XiYa-Ping MaoQiang HouDao-Li YuanZi-Ding ZhangWei-Quan Liu Source Type: research

Profiling the tyrosine phosphoproteome of different mouse mammary tumour models reveals distinct, model-specific signaling networks and conserved oncogenic pathways
Conclusion: This study provides network-level insights into signaling in these breast cancer models and demonstrates that comparative phosphoproteomics can identify conserved oncogenic signaling pathways. The Met-amplified, p53-null tumours provide a new pre-clinical model for a subset of triple-negative breast cancers.
Source: Breast Cancer Research - September 9, 2014 Category: Cancer & Oncology Authors: Naveid AliJianmin WuFalko HochgräfeHoward ChanRadhika NairSunny YeLuxi ZhangRuth LyonsMark PineseHong LeeNicola ArmstrongChristopher OrmandySusan ClarkAlexander SwarbrickRoger Daly Source Type: research

Targeted siRNA-immunoliposomes as a promising therapeutic agent against highly pathogenic avian influenza A (H5N1) virus infection.
This study describes a proof of concept study on the use of siRNA-immunoliposomes as a therapeutic agent against H5N1 influenza virus infection. SiRNA specific for influenza virus nucleoprotein (NP) mRNA was employed as the key antiviral agent to inhibit viral replication in this study. A humanized single-chain Fv antibody (huscFv) against the hemagglutinin (HA) of H5N1 highly pathogenic avian influenza (HPAI) was used as the targeting molecule to HA of H5N1 virus, which is abundantly expressed on the surface of infected cells. The huscFv was decorated onto cationic PEGylated DC-Chol/DOPE liposomes to generate immunoliposo...
Source: Antimicrobial Agents and Chemotherapy - March 10, 2014 Category: Microbiology Authors: Khantasup K, Kopermsub P, Chaichoun K, Dharakul T Tags: Antimicrob Agents Chemother Source Type: research

Autophagy mediates avian influenza H5N1 pseudotyped particle-induced lung inflammation through NF-{kappa}B and p38 MAPK signaling pathways
Since avian influenza virus H5N1-induced hypercytokemia plays a key role in acute lung injury, understanding its molecular mechanism is highly desirable for discovering therapeutic targets against H5N1 infection. In the present study, we investigated the role of autophagy in H5N1-induced lung inflammation by using H5N1 pseudotyped viral particles (H5N1pps). The results showed that H5N1pps significantly induced autophagy both in A549 human lung epithelial cells and in mouse lung tissues, which was primarily due to hemagglutinin (HA) of H5N1 virus. Blocking autophagy with 3-methyladenine (an autophagy inhibitor) or siRNA kno...
Source: AJP: Lung Cellular and Molecular Physiology - January 15, 2014 Category: Respiratory Medicine Authors: Pan, H., Zhang, Y., Luo, Z., Li, P., Liu, L., Wang, C., Wang, H., Li, H., Ma, Y. Tags: ARTICLES Source Type: research

Autophagy mediates avian influenza H5N1 pseudotyped particle-induced lung inflammation through NF-κB and p38 MAPK signaling pathways.
Abstract Since avian influenza virus H5N1-induced hypercytokemia plays a key role in acute lung injury, understanding its molecular mechanism is highly desirable for discovering therapeutic targets against H5N1 infection. In the present study, we investigated the role of autophagy in H5N1-induced lung inflammation by using H5N1 hemagglutinin pseudotyped viral particles (H5N1pps). The results showed that H5N1pps significantly induced autophagy both in A549 human lung epithelial cells and in mouse lung tissues, which was primarily due to hemagglutinin (HA) of H5N1 virus. Blocking autophagy with 3-MA (an autophagy in...
Source: Am J Physiol Lung Ce... - November 15, 2013 Category: Respiratory Medicine Authors: Pan H, Zhang Y, Luo Z, Li P, Liu L, Wang C, Wang H, Li H, Ma Y Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Expression, function and regulation of Evi-1 during embryonic avian development.
Abstract Ecotropical viral integration site 1 (Evi-1) is a transcription factor essential for vascularisation and cell proliferation during embryonic development. The chimeric transcription factor AML1-EVI-1 is activated in leukaemia where it plays a role as a differentiation block and stimulator of proliferation. Here, we cloned chicken Evi-1 and analysed its expression during embryonic development. There was early expression in the pharyngeal arches, in the brain and intermediate mesoderm of chicken embryos at stage 15. Later at stage 20, Evi-1 mesenchymal expression was concentrated in the second pharyngeal arc...
Source: Gene Expression Patterns : GEP - July 3, 2013 Category: Genetics & Stem Cells Authors: Celá P, Balková S, Bryjová A, Horaková D, Míšek I, Richman JM, Buchtová M Tags: Gene Expr Patterns Source Type: research