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Infectious Disease: Bird Flu

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Total 56 results found since Jan 2013.

ErbB4 protects against neuronal apoptosis via activation of YAP/PIK3CB signaling pathway in a rat model of subarachnoid hemorrhage.
In this study, we explored the neuroprotective effects of ErbB4 and its downstream YAP (yes-associated protein)/PIK3CB signaling pathway in early brain injury after SAH in a rat model using the endovascular perforation method. Rats were neurologically evaluated with the Modified Garcia Scale and beam balance test at 24h and 72h after SAH. An ErbB4 activator Neuregulin 1β1 (Nrg 1β1), ErbB4 siRNA and YAP siRNA were used to explore this pathway. The expression of p-ErbB4 and YAP was significantly increased after SAH. Multiple immunofluorescence labeling experiments demonstrated that ErbB4 is mainly expressed in neurons. Act...
Source: Experimental Neurology - July 26, 2017 Category: Neurology Authors: Yan F, Tan X, Wan W, Dixon BJ, Fan R, Enkhjargal B, Li Q, Zhang J, Chen G, Zhang JH Tags: Exp Neurol Source Type: research

Molecular characterization, expression of chicken TBK1 gene and its effect on IRF3 signaling pathway
In conclusion, our results demonstrated that chTBK1 may be an important immunoregulator for IRF3 and IFN β induction in response to viral stimulation in chicken.
Source: PLoS One - May 11, 2017 Category: Biomedical Science Authors: Yan Wang Source Type: research

H7N9 Influenza A Virus Exhibits Importin- α7–Mediated Replication in the Mammalian Respiratory Tract
The acute respiratory distress syndrome (ARDS) is the leading cause of death in influenza A virus (IAV) –infected patients. Hereby, the cellular importin-α7 gene plays a major role. It promotes viral replication in the lung, thereby increasing the risk for the development of pneumonia complicated by ARDS. Herein, we analyzed whether the recently emerged H7N9 avian IAV has already adapted to human i mportin-α7 use, which is associated with high-level virus replication in the mammalian lung. Using a cell-based viral polymerase activity assay, we could detect a decreased H7N9 IAV polymerase activity when importin-α7 was silenced by siRNA.
Source: American Journal of Pathology - February 8, 2017 Category: Pathology Authors: Stephanie Bertram, Swantje Thiele, Carola Dreier, Patricia Resa-Infante, Annette Preu ß, Debby van Riel, Chris K.P. Mok, Folker Schwalm, Joseph S.M. Peiris, Hans-Dieter Klenk, Gülsah Gabriel Tags: Regular Article Source Type: research

H7N9 Influenza A Virus Exhibits Importin- α7-Mediated Replication in the Mammalian Respiratory Tract.
H7N9 Influenza A Virus Exhibits Importin-α7-Mediated Replication in the Mammalian Respiratory Tract. Am J Pathol. 2017 Feb 08;: Authors: Bertram S, Thiele S, Dreier C, Resa-Infante P, Preuß A, van Riel D, Mok CK, Schwalm F, Peiris JS, Klenk HD, Gabriel G Abstract The acute respiratory distress syndrome (ARDS) is the leading cause of death in influenza A virus (IAV)-infected patients. Hereby, the cellular importin-α7 gene plays a major role. It promotes viral replication in the lung, thereby increasing the risk for the development of pneumonia complicated by ARDS. Herein, we analyzed whether the rec...
Source: The American Journal of Pathology - February 7, 2017 Category: Pathology Authors: Bertram S, Thiele S, Dreier C, Resa-Infante P, Preuß A, van Riel D, Mok CK, Schwalm F, Peiris JS, Klenk HD, Gabriel G Tags: Am J Pathol Source Type: research

Avian leukosis virus subgroup J induces VEGF expression via NF- κB/PI3K-dependent IL-6 production.
Avian leukosis virus subgroup J induces VEGF expression via NF-κB/PI3K-dependent IL-6 production. Oncotarget. 2016 Nov 10;: Authors: Gao Y, Zhang Y, Yao Y, Guan X, Liu Y, Qi X, Wang Y, Liu C, Zhang Y, Gao H, Nair V, Wang X, Gao Y Abstract Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus causing hemangiomas and myeloid tumors in chickens. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory interleukin involved in many types of cancer. We previously demonstrated that IL-6 expression was induced following ALV-J infection in chickens. The aim of this study is to characterize the mechan...
Source: Oncotarget - November 18, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells
AbstractWe previously identified chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J (ALV-J), using a DF1 cell line expressing the viral envelope (env) protein. To further probe whether other proteins participate in virus infection, we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env. Mass spectrometry analysis indicates that the chicken glucose-regulation protein 78 (chGRP78) of the DF1 membrane interacted with the ALV-J env protein. The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J in...
Source: Veterinary Research - September 5, 2016 Category: Veterinary Research Source Type: research

Overexpression of MYCN promotes proliferation of non-small cell lung cancer
Abstract V-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN) is an oncogene that is known amplified and overexpressed in different human malignancies including small cell lung cancer. However, the role of MYCN in non-small cell lung cancer (NSCLC) development remains elusive. In the present study, Western blot and immunohistochemistry assays demonstrated that MYCN was overexpressed in NSCLC tumor tissues and cell lines. In addition, immunohistochemistry analysis revealed that upregulation of MYCN expression was positively correlated with a more invasive tumor phenotype and poor prognosis. In v...
Source: Tumor Biology - July 22, 2016 Category: Cancer & Oncology Source Type: research

Hemagglutinin of influenza A virus binds specifically to cell surface nucleolin and plays a role in virus internalization.
Abstract The hemagglutinin (HA) protein of influenza A virus initiates cell entry by binding to sialic acids on target cells. In the current study, we demonstrated that in addition to sialic acids, influenza A/Puerto Rico/8/34 H1N1 (PR8) virus HA specifically binds to cell surface nucleolin (NCL). The interaction between HA and NCL was initially revealed with virus overlay protein binding assay (VOPBA) and subsequently verified with co-immunoprecipitation. Importantly, inhibiting cell surface NCL with NCL antibody, blocking PR8 viruses with purified NCL protein, or depleting endogenous NCL with siRNA all substanti...
Source: Virology - April 12, 2016 Category: Virology Authors: Chan CM, Chu H, Zhang AJ, Leung LH, Sze KH, Kao RY, Chik KK, To KK, Chan JF, Chen H, Jin DY, Liu L, Yuen KY Tags: Virology Source Type: research

Targeting host calpain proteases decreases influenza A virus infection
Influenza A viruses (IAV) trigger contagious acute respiratory diseases. A better understanding of the molecular mechanisms of IAV pathogenesis and host immune responses is required for the development of more efficient treatments of severe influenza. Calpains are intracellular proteases that participate in diverse cellular responses, including inflammation. Here, we used in vitro and in vivo approaches to investigate the role of calpain signaling in IAV pathogenesis. Calpain expression and activity were found altered in IAV-infected bronchial epithelial cells. With the use of small-interfering RNA (siRNA) gene silencing, ...
Source: AJP: Lung Cellular and Molecular Physiology - March 31, 2016 Category: Respiratory Medicine Authors: Blanc, F., Furio, L., Moisy, D., Yen, H.-L., Chignard, M., Letavernier, E., Naffakh, N., Mok, C. K. P., Si-Tahar, M. Tags: ARTICLES Source Type: research

Upregulation of miRNA-155 expression by OxLDL in dendritic cells involves JAK1/2 kinase and transcription factors YY1 and MYB.
Abstract Dendritic cells (DCs) have been implicated in the pathogenesis of atherosclerosis (AS). Whereas the pathogenic role of oxidized low-density lipoprotein (oxLDL) in the development and progression of AS has been recognized previously, the contribution of microRNA-155 (miR-155) to AS was previously not fully understood. It had also been noted that miR-155 levels were upregulated by oxLDL in various cell types under different (patho)physiological conditions, but its underlying mechanisms had not been examiend in detail. Thus, in the present study, we observed that oxLDL treatment increased miR-155 express...
Source: International Journal of Molecular Medicine - March 11, 2016 Category: Molecular Biology Authors: Yan H, Wang S, Li Z, Zhao W, Wang Z, Sun Z, Pan Y, Zhu J Tags: Int J Mol Med Source Type: research

Functional analysis of the TMPRSS2:ERG fusion gene in cisplatin‑induced cell death.
Authors: Wu J, Chi L, Chen Z, Lu X, Xiao S, Zhang G, Luo J, Chen GM, Yang J Abstract The TMPRSS2:E‑twenty‑six (ETS) gene fusion occurs frequently in a high proportion of patients with prostate cancer (PCa) in Western countries, and the aberrant expression of TMPRSS2: v‑ETS avian erythroblastosis virus E26 oncogene homolog (ERG), the most common form of the corresponding protein, can regulate cell migration and contribute to tumor invasion and metastasis. However, its association with other cellular events, and in particular, cell death, remain unknown. To examine the function of such fusion genes, an expressi...
Source: Molecular Medicine Reports - March 6, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Targeting host calpain proteases decreases influenza A virus infection.
Abstract Influenza A viruses (IAV) trigger contagious acute respiratory diseases. A better understanding of the molecular mechanisms of IAV pathogenesis and host immune responses is required for the development of more efficient treatments of severe influenza. Calpains are intracellular proteases that participate to diverse cellular responses, including inflammation. Here, we used in vitro and in vivo approaches to investigate the role of calpains signalling in IAV pathogenesis. Calpain expression and activity were found altered in IAV-infected bronchial epithelial cells. Using siRNA gene silencing, specific synth...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 8, 2016 Category: Cytology Authors: Blanc F, Furio L, Moisy D, Yen HL, Chignard M, Letavernier E, Naffakh N, Mok CK, Si-Tahar M Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

A critical role of LAMP-1 in avian reovirus P10 degradation associated with inhibition of apoptosis and virus release
We report here the identification of cellular lysosome-associated membrane protein 1 (LAMP-1) as an interaction partner of p10 by yeast two-hybrid screening, immunoprecipitation and confocal microscopy assays. We found that rapid degradation of p10 was associated with ubiquitination. Importantly, ARV p10 degradation in host cells could be completely abolished by knockdown of LAMP-1 by siRNA, indicating that LAMP-1 is required for ARV p10 degradation in host cells. In contrast, overexpression of LAMP-1 facilitated p10 degradation. Furthermore, knockdown of LAMP-1 allowed p10 accumulation, enhancing p10-induced apoptosis and...
Source: Archives of Virology - January 7, 2016 Category: Virology Source Type: research

Expression of a single siRNA against a conserved region of NP gene strongly inhibits in vitro replication of different Influenza A virus strains of avian and swine origin
In conclusion, these findings reveal new siRNA sequences able to inhibit Influenza A virus replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection both in humans and animals.
Source: Antiviral Therapy - May 17, 2015 Category: Virology Source Type: research

siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus.
In this study, we have designed three siRNAs (PB2-2235, PB2-479 and NP-865) targeting PB2 and NP genes of avian influenza virus and evaluated their potential, measured by hemagglutination (HA), plaque reduction and Real time RT-PCR assay, in inhibiting H5N1 virus (A/chicken/Navapur/7972/2006) replication in MDCK cells. The siRNAs caused 8- to 16-fold reduction in virus HA titers at 24 h after challenged with 100TCID50 of virus. Among these siRNAs, PB2-2235 offered the highest inhibition of virus replication with 16-fold reduction in virus HA titer, 80 percent reduction in viral plaque counts and 94 percent inhibition in ex...
Source: Journal of Biosciences - May 16, 2015 Category: Biomedical Science Authors: Behera P, Nagarajan S, Murugkar HV, Kalaiyarasu S, Prakash A, Gothalwal R, Dubey SC, Kulkarni DD, Tosh C Tags: J Biosci Source Type: research