Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light.

Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light. Chronobiol Int. 2020 Oct 28;:1-16 Authors: Bian J, Wang Z, Dong Y, Cao J, Chen Y Abstract As the circadian pacemaker of birds, the retina possesses the ability to receive light information, generate circadian oscillation, and secrete melatonin. Previous studies have confirmed that monochromatic green light can accelerate the circadian rhythmic expression of clock genes in the chick retina, thereby increasing cAanat mRNA level and melatonin secretion. However, as the core components of the transcriptional-translational negative feedback loop, the role that cBmal1 and cClock plays in the regulation of the retinal molecular clock system and melatonin secretion under monochromatic green light is unknown. To explore their in these processes, embryonic chick retinal cells at six embryo ages were isolated and cultured under light-dark (LD) 12:12 monochromatic green light with, and the role of cBmal1 and cClock in the regulation of the retinal molecular clock and melatonin secretion in the chick retina was explored by siRNA interference and overexpression. The results showed siRNA interference and overexpression of cBmal1 obliterated the circadian rhythm of cCry1, cPer2, cPer3, cAanat, and melatonin secretion. Moreover, the siRNA interference of cBmal1 significantly reduced the average expression levels of the positive clock gene...
Source: Chronobiology International - Category: Biology Authors: Tags: Chronobiol Int Source Type: research