Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s
J Inorg Biochem. 2023 Apr 28;245:112241. doi: 10.1016/j.jinorgbio.2023.112241. Online ahead of print.ABSTRACTCytochromes P450 (CYP), enzymes involved in the metabolism of endogenous and xenobiotic substrates, provide an excellent model system to study how membrane proteins with unique functions have catalytically adapted through evolution. Molecular adaptation of deep-sea proteins to high hydrostatic pressure remains poorly understood. Herein, we have characterized recombinant cytochrome P450 sterol 14α-demethylase (CYP51), an essential enzyme of cholesterol biosynthesis, from an abyssal fish species, Coryphaenoides armat...
Source: Journal of Inorganic Biochemistry - May 20, 2023 Category: Biochemistry Authors: Jared V Goldstone David C Lamb Steven L Kelly Galina I Lepesheva John J Stegeman Source Type: research

Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s
J Inorg Biochem. 2023 Apr 28;245:112241. doi: 10.1016/j.jinorgbio.2023.112241. Online ahead of print.ABSTRACTCytochromes P450 (CYP), enzymes involved in the metabolism of endogenous and xenobiotic substrates, provide an excellent model system to study how membrane proteins with unique functions have catalytically adapted through evolution. Molecular adaptation of deep-sea proteins to high hydrostatic pressure remains poorly understood. Herein, we have characterized recombinant cytochrome P450 sterol 14α-demethylase (CYP51), an essential enzyme of cholesterol biosynthesis, from an abyssal fish species, Coryphaenoides armat...
Source: Journal of Inorganic Biochemistry - May 20, 2023 Category: Biochemistry Authors: Jared V Goldstone David C Lamb Steven L Kelly Galina I Lepesheva John J Stegeman Source Type: research

Structural modeling of cytochrome P450 51 from a deep-sea fish points to a novel structural feature in other CYP51s
J Inorg Biochem. 2023 Apr 28;245:112241. doi: 10.1016/j.jinorgbio.2023.112241. Online ahead of print.ABSTRACTCytochromes P450 (CYP), enzymes involved in the metabolism of endogenous and xenobiotic substrates, provide an excellent model system to study how membrane proteins with unique functions have catalytically adapted through evolution. Molecular adaptation of deep-sea proteins to high hydrostatic pressure remains poorly understood. Herein, we have characterized recombinant cytochrome P450 sterol 14α-demethylase (CYP51), an essential enzyme of cholesterol biosynthesis, from an abyssal fish species, Coryphaenoides armat...
Source: Journal of Inorganic Biochemistry - May 20, 2023 Category: Biochemistry Authors: Jared V Goldstone David C Lamb Steven L Kelly Galina I Lepesheva John J Stegeman Source Type: research

The Impact of Surface Area per Volume (SA/V) Ratio on Drug Transport from Supersaturated Solutions of Ketoconazole
Eur J Pharm Biopharm. 2023 May 16:S0939-6411(23)00138-8. doi: 10.1016/j.ejpb.2023.05.016. Online ahead of print.ABSTRACTThe purpose of this study aimed to evaluate the impact of the surface area per volume (SA/V) ratio on drug transport from two supersaturated solutions (SSs) of ketoconazole with and without hydroxypropyl methylcellulose (HPMC), used as a precipitation inhibitor. In vitro dissolution, membrane permeation with two SA/V ratios, and in vivo absorption profiles for both SSs were determined. For the SS without HPMC, a two-step precipitation process due to the liquid-liquid phase separation was observed; the con...
Source: European Journal of Pharmaceutics and Biopharmaceutics - May 18, 2023 Category: Drugs & Pharmacology Authors: Haruki Higashino Corey Develin Chie Higashino Tyler Lim Blair Miezeiewski Robert Strab Rachana Patel Siddhartha Bhoopathy Ismael Hidalgo Source Type: research

Isolation and Identification of Flavanone Derivative Eriodictyol from the Methanol Extract of < em > Afzelia africana < /em > Bark and Its Antimicrobial and Antioxidant Activities
CONCLUSION: The study reports for the first time the identification of eriodictyol from the bark of A. africana which exhibited significant antioxidant and antimicrobial properties.PMID:37200890 | PMC:PMC10188263 | DOI:10.1155/2023/9345047 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - May 18, 2023 Category: Complementary Medicine Authors: Bright Yaw Vigbedor Clement Osei Akoto David Neglo Source Type: research

The Impact of Surface Area per Volume (SA/V) Ratio on Drug Transport from Supersaturated Solutions of Ketoconazole
Eur J Pharm Biopharm. 2023 May 16:S0939-6411(23)00138-8. doi: 10.1016/j.ejpb.2023.05.016. Online ahead of print.ABSTRACTThe purpose of this study aimed to evaluate the impact of the surface area per volume (SA/V) ratio on drug transport from two supersaturated solutions (SSs) of ketoconazole with and without hydroxypropyl methylcellulose (HPMC), used as a precipitation inhibitor. In vitro dissolution, membrane permeation with two SA/V ratios, and in vivo absorption profiles for both SSs were determined. For the SS without HPMC, a two-step precipitation process due to the liquid-liquid phase separation was observed; the con...
Source: European Journal of Pharmaceutics and Biopharmaceutics - May 18, 2023 Category: Drugs & Pharmacology Authors: Haruki Higashino Corey Develin Chie Higashino Tyler Lim Blair Miezeiewski Robert Strab Rachana Patel Siddhartha Bhoopathy Ismael Hidalgo Source Type: research

Isolation and Identification of Flavanone Derivative Eriodictyol from the Methanol Extract of < em > Afzelia africana < /em > Bark and Its Antimicrobial and Antioxidant Activities
CONCLUSION: The study reports for the first time the identification of eriodictyol from the bark of A. africana which exhibited significant antioxidant and antimicrobial properties.PMID:37200890 | PMC:PMC10188263 | DOI:10.1155/2023/9345047 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - May 18, 2023 Category: Complementary Medicine Authors: Bright Yaw Vigbedor Clement Osei Akoto David Neglo Source Type: research

Ketoconazole as second-line treatment for Cushing ’s disease after transsphenoidal surgery: systematic review and meta-analysis
ConclusionKetoconazole can be considered a safe and efficacious option for Cushing’s disease treatment after pituitary surgery.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/#searchadvanced, (CRD42022308041). (Source: Frontiers in Endocrinology)
Source: Frontiers in Endocrinology - May 8, 2023 Category: Endocrinology Source Type: research