Sporotrichosis Caused by Non-Wild Type Sporothrix brasiliensis Strains
This study evaluated 100 Sporothrix spp. strains obtained from 1999 to 2018 from the Evandro Chagas National Institute of Infectious Diseases, Fiocruz, which were identified through a polymerase chain reaction using specific primers for species identification. Two-fold serial dilutions of stock solutions of amphotericin B, itraconazole, posaconazole, ketoconazole and terbinafine prepared in dimethyl sulfoxide were performed to obtain working concentrations of antifungal drugs ranging from 0.015 to 8.0 mg/L. The broth microdilution reference method was performed according the M38-A2 CLSI guideline. All strains were identifi...
Source: Frontiers in cellular and infection microbiology - May 27, 2022 Category: Microbiology Source Type: research

Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A
DISCUSSION: In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.PMID:35609738 | DOI:10.1016/j.fct.2022.113166 (Source: Food and Chemical Toxicology)
Source: Food and Chemical Toxicology - May 24, 2022 Category: Food Science Authors: Anton Vremere Carmine Merola Federico Fanti Manuel Sergi Monia Perugini Dario Compagnone Milena Mikhail Stefano Lorenzetti Michele Amorena Source Type: research

Pesticides Inhibit Retinoic Acid Catabolism in PLHC-1 and ZFL Fish Hepatic Cell Lines
Chem Res Toxicol. 2022 May 24. doi: 10.1021/acs.chemrestox.2c00050. Online ahead of print.ABSTRACTThe population of yellow perch (Perca flavescens) in lake Saint-Pierre (QC, Canada) has been dramatically declining since 1995 without any sign of recovery. Previous studies have shown disrupted retinoid (vitamin A) metabolic pathways in these fish, possibly due to the influence of pesticides. Our study aimed to evaluate the impact of some herbicides and neonicotinoids on retinoic acid catabolism in the fish hepatic cell lines PLHC-1 and ZFL. We hypothesized that pesticides accelerate the catabolism of retinoic acid through ox...
Source: Cell Research - May 24, 2022 Category: Cytology Authors: Charbel Hanna Monique Boily Catherine Jumarie Source Type: research

Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A
DISCUSSION: In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.PMID:35609738 | DOI:10.1016/j.fct.2022.113166 (Source: Food and Chemical Toxicology)
Source: Food and Chemical Toxicology - May 24, 2022 Category: Food Science Authors: Anton Vremere Carmine Merola Federico Fanti Manuel Sergi Monia Perugini Dario Compagnone Milena Mikhail Stefano Lorenzetti Michele Amorena Source Type: research

Prediction of In Vitro Drug Dissolution into Fasted-state Biorelevant Media: Contributions of Solubility Enhancement and Relatively Low Colloid Diffusivity
Eur J Pharm Sci. 2022 May 13:106210. doi: 10.1016/j.ejps.2022.106210. Online ahead of print.ABSTRACTA previously developed model showed good predictability for in vitro drug dissolution into FeSSGF and FeSSIF-V2, where biorelevant media-mediated enhanced drug dissolution by only about one-tenth as much as it enhanced solubility, due to drug-loaded fat globules and mixed micelles exhibiting far slower diffusivity than free drug. The present objective was to quantitatively assess the contributions of fasted biorelevant media-mediated solubility and diffusivity on enhanced drug dissolution in FaSSGF and FaSSIF-V2. Griseofulvi...
Source: European Journal of Pharmaceutical Sciences - May 16, 2022 Category: Drugs & Pharmacology Authors: Raqeeb Jamil James E Polli Source Type: research