Chemicals of environmental concern as inhibitors of human placental 3 β-hydroxysteroid dehydrogenase 1 and aromatase: Screening and docking analysis
In conclusion, 17β-estradiol, diethylstilbestrol, bisphenol A, and bisphenol AF are human HSD3B1 inhibitors, and ketoconazole, zearalenone, diethylstilbestrol, bisphenol A, and bisphenol AF are human CYP19A1 inhibitors.PMID:36374910 | DOI:10.1016/j.cbi.2022.110243 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - November 14, 2022 Category: Molecular Biology Authors: Luming Tang Lei Shi Yunbing Tang Yingfen Ying Yaoyao Dong Huitao Li Ren-Shan Ge Source Type: research
Chemicals of environmental concern as inhibitors of human placental 3 β-hydroxysteroid dehydrogenase 1 and aromatase: Screening and docking analysis
In conclusion, 17β-estradiol, diethylstilbestrol, bisphenol A, and bisphenol AF are human HSD3B1 inhibitors, and ketoconazole, zearalenone, diethylstilbestrol, bisphenol A, and bisphenol AF are human CYP19A1 inhibitors.PMID:36374910 | DOI:10.1016/j.cbi.2022.110243 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - November 14, 2022 Category: Molecular Biology Authors: Luming Tang Lei Shi Yunbing Tang Yingfen Ying Yaoyao Dong Huitao Li Ren-Shan Ge Source Type: research
Chemicals of environmental concern as inhibitors of human placental 3 β-hydroxysteroid dehydrogenase 1 and aromatase: Screening and docking analysis
In conclusion, 17β-estradiol, diethylstilbestrol, bisphenol A, and bisphenol AF are human HSD3B1 inhibitors, and ketoconazole, zearalenone, diethylstilbestrol, bisphenol A, and bisphenol AF are human CYP19A1 inhibitors.PMID:36374910 | DOI:10.1016/j.cbi.2022.110243 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - November 14, 2022 Category: Molecular Biology Authors: Luming Tang Lei Shi Yunbing Tang Yingfen Ying Yaoyao Dong Huitao Li Ren-Shan Ge Source Type: research
GSE217187 Interplay between acetylation and ubiquitination of Isw1 confers multidrug resistance in Cryptococcus neoformans
Contributor : Chen DingSeries Type : Expression profiling by high throughput sequencingOrganism : Cryptococcus neoformans var. grubii H99Cryptococcus neoformans poses a great threat to human communities, given that it quickly becomes resistant to available antifungal drugs. Herein, a conserved chromatin remodeler, Isw1, is shown to function as a master transcriptional modulator of genes responsible for multidrug resistance. It reciprocally controls drug resistance, and cells with disrupted ISW1 demonstrate profound resistance to fluconazole, ketoconazole, 5-fluorocytosine and 5-fluorouracil. Mass spectrometry reveals that...
Source: GEO: Gene Expression Omnibus - November 10, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Cryptococcus neoformans var. grubii H99 Source Type: research
Treatment of Primary Pigmented Nodular Adrenocortical
Disease
Horm Metab Res 2022; 54: 721-730 DOI: 10.1055/a-1948-6990Primary pigmented nodular adrenocortical disease (PPNAD) is a rare cause of
adrenocorticotropin hormone (ACTH)-independent Cushing’s syndrome (CS),
which mainly occurs in children and young adults. Treatment options with proven
clinical efficacy for PPNAD include adrenalectomy (bilateral or unilateral
adrenalectomy) and drug treatment to control hypercortisolemia. Previously, the
main treatment of PPNAD is bilateral adrenal resection and long-term hormone
replacement after surgery. In recent years, cases r...
Source: Hormone and Metabolic Research - November 10, 2022 Category: Endocrinology Authors: Liu, Xinming Zhang, Siwen Guo, Yunran Gang, Xiaokun Wang, Guixia Tags: Review Source Type: research
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Arch Toxicol. 2022 Oct 23. doi: 10.1007/s00204-022-03394-z. Online ahead of print.ABSTRACTPyrrolizidine alkaloids (PAs) are secondary plant metabolites, which can be found as contaminant in various foods and herbal products. Several PAs can cause hepatotoxicity and liver cancer via damaging hepatic sinusoidal endothelial cells (HSECs) after hepatic metabolization. HSECs themselves do not express the required metabolic enzymes for activation of PAs. Here we applied a co-culture model to mimic the in vivo hepatic environment and to study PA-induced effects on not metabolically active neighbour cells. In this co-culture model...
Source: Archives of Toxicology - October 23, 2022 Category: Toxicology Authors: Naji Said Aboud Hadi Ezgi Eyluel Bankoglu Helga Stopper Source Type: research
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Arch Toxicol. 2022 Oct 23. doi: 10.1007/s00204-022-03394-z. Online ahead of print.ABSTRACTPyrrolizidine alkaloids (PAs) are secondary plant metabolites, which can be found as contaminant in various foods and herbal products. Several PAs can cause hepatotoxicity and liver cancer via damaging hepatic sinusoidal endothelial cells (HSECs) after hepatic metabolization. HSECs themselves do not express the required metabolic enzymes for activation of PAs. Here we applied a co-culture model to mimic the in vivo hepatic environment and to study PA-induced effects on not metabolically active neighbour cells. In this co-culture model...
Source: Archives of Toxicology - October 23, 2022 Category: Toxicology Authors: Naji Said Aboud Hadi Ezgi Eyluel Bankoglu Helga Stopper Source Type: research
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Arch Toxicol. 2022 Oct 23. doi: 10.1007/s00204-022-03394-z. Online ahead of print.ABSTRACTPyrrolizidine alkaloids (PAs) are secondary plant metabolites, which can be found as contaminant in various foods and herbal products. Several PAs can cause hepatotoxicity and liver cancer via damaging hepatic sinusoidal endothelial cells (HSECs) after hepatic metabolization. HSECs themselves do not express the required metabolic enzymes for activation of PAs. Here we applied a co-culture model to mimic the in vivo hepatic environment and to study PA-induced effects on not metabolically active neighbour cells. In this co-culture model...
Source: Archives of Toxicology - October 23, 2022 Category: Toxicology Authors: Naji Said Aboud Hadi Ezgi Eyluel Bankoglu Helga Stopper Source Type: research
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Arch Toxicol. 2022 Oct 23. doi: 10.1007/s00204-022-03394-z. Online ahead of print.ABSTRACTPyrrolizidine alkaloids (PAs) are secondary plant metabolites, which can be found as contaminant in various foods and herbal products. Several PAs can cause hepatotoxicity and liver cancer via damaging hepatic sinusoidal endothelial cells (HSECs) after hepatic metabolization. HSECs themselves do not express the required metabolic enzymes for activation of PAs. Here we applied a co-culture model to mimic the in vivo hepatic environment and to study PA-induced effects on not metabolically active neighbour cells. In this co-culture model...
Source: Archives of Toxicology - October 23, 2022 Category: Toxicology Authors: Naji Said Aboud Hadi Ezgi Eyluel Bankoglu Helga Stopper Source Type: research
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Arch Toxicol. 2022 Oct 23. doi: 10.1007/s00204-022-03394-z. Online ahead of print.ABSTRACTPyrrolizidine alkaloids (PAs) are secondary plant metabolites, which can be found as contaminant in various foods and herbal products. Several PAs can cause hepatotoxicity and liver cancer via damaging hepatic sinusoidal endothelial cells (HSECs) after hepatic metabolization. HSECs themselves do not express the required metabolic enzymes for activation of PAs. Here we applied a co-culture model to mimic the in vivo hepatic environment and to study PA-induced effects on not metabolically active neighbour cells. In this co-culture model...
Source: Archives of Toxicology - October 23, 2022 Category: Toxicology Authors: Naji Said Aboud Hadi Ezgi Eyluel Bankoglu Helga Stopper Source Type: research
Strong Pharmacokinetic Drug-Drug Interactions With Drugs Approved by the US Food and Drug Administration in 2021: Mechanisms and Clinical Implications
Clin Ther. 2022 Oct 6:S0149-2918(22)00323-X. doi: 10.1016/j.clinthera.2022.09.009. Online ahead of print.ABSTRACTPURPOSE: This analysis aimed to identify all strong drug-drug interactions (DDIs) associated with drugs approved by the US Food and Drug Administration (FDA) in 2021.METHODS: DDI data for small molecular drugs approved by the FDA in 2021 (N = 36) were analyzed using the University of Washington Drug Interaction Database. The mechanism(s) and clinical magnitude of these interactions were characterized based on information available in the new drug application reviews. Clinical studies and simulation results with ...
Source: Clinical Therapeutics - October 9, 2022 Category: Drugs & Pharmacology Authors: Jingjing Yu Yan Wang Isabelle Ragueneau-Majlessi Source Type: research
Strong Pharmacokinetic Drug-Drug Interactions With Drugs Approved by the US Food and Drug Administration in 2021: Mechanisms and Clinical Implications
Clin Ther. 2022 Oct 6:S0149-2918(22)00323-X. doi: 10.1016/j.clinthera.2022.09.009. Online ahead of print.ABSTRACTPURPOSE: This analysis aimed to identify all strong drug-drug interactions (DDIs) associated with drugs approved by the US Food and Drug Administration (FDA) in 2021.METHODS: DDI data for small molecular drugs approved by the FDA in 2021 (N = 36) were analyzed using the University of Washington Drug Interaction Database. The mechanism(s) and clinical magnitude of these interactions were characterized based on information available in the new drug application reviews. Clinical studies and simulation results with ...
Source: Clinical Therapeutics - October 9, 2022 Category: Drugs & Pharmacology Authors: Jingjing Yu Yan Wang Isabelle Ragueneau-Majlessi Source Type: research
Strong Pharmacokinetic Drug-Drug Interactions With Drugs Approved by the US Food and Drug Administration in 2021: Mechanisms and Clinical Implications
Clin Ther. 2022 Oct 6:S0149-2918(22)00323-X. doi: 10.1016/j.clinthera.2022.09.009. Online ahead of print.ABSTRACTPURPOSE: This analysis aimed to identify all strong drug-drug interactions (DDIs) associated with drugs approved by the US Food and Drug Administration (FDA) in 2021.METHODS: DDI data for small molecular drugs approved by the FDA in 2021 (N = 36) were analyzed using the University of Washington Drug Interaction Database. The mechanism(s) and clinical magnitude of these interactions were characterized based on information available in the new drug application reviews. Clinical studies and simulation results with ...
Source: Clinical Therapeutics - October 9, 2022 Category: Drugs & Pharmacology Authors: Jingjing Yu Yan Wang Isabelle Ragueneau-Majlessi Source Type: research
Evaluation of ketoconazole as a treatment for Cushing ’s disease in a retrospective cohort
ConclusionKetoconazole effectively controlled hypercortisolism in 66% of Cushing’s disease patients, being a relatively safe drug for those without remission after transsphenoidal surgery or whose symptoms must be controlled until a new definitive therapy is carried out. Hypokalemia is a frequent metabolic effect not yet described in other series, which should be monitored during treatment. (Source: Frontiers in Endocrinology)
Source: Frontiers in Endocrinology - October 7, 2022 Category: Endocrinology Source Type: research
Development and validation of a sensitive LC-MS/MS method for determination of intracellular concentration of fluconazole in Candida albicans
In this study, a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to quantify the intracellular fluconazole (FLC) content in the opportunistic human fungal pathogen Candida albicans. The cell lysates were prepared by lysing C. albicans cells with Precellys homogenizers and FLC was extracted with methylene chloride. The entire extraction approach was simple, precise and reliable. The extracts were separated on a Zorbax SB-C18 column using a mobile phase of acetonitrile (solvent A) and deionized water plus 0.1% formic acid. FLC and ketoconazole (KCZ, internal standard) ...
Source: Frontiers in Microbiology - October 5, 2022 Category: Microbiology Source Type: research
