Study on morphological and genetic diversity of Rehmannia glutinosa cultivated in Japan
In this study, we clarified the diversity ofR. glutinosa cultivated in Japan on the basis of morphological observation and genetic analysis. We conducted principal component analysis (PCA) ofR. glutinosa morphology, including leaf surface color, leaf undersurface anthocyanin coloration, root shape, and the ratio of string root. We also performed (1) sequence-related amplified polymorphism (SRAP) analysis and (2) polymorphism analysis of theTEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) gene region. We were able to separate Akaya type from Kaikei type, and to divide Kaikei type into three small groups. These two gene analysis...
Source: Journal of Natural Medicines - December 3, 2021 Category: Drugs & Pharmacology Source Type: research
Shin ’iseihaito (Xinyiqingfeitang) extract ameliorates ovalbumin-induced murine allergic rhinitis by regulating cytokines
We examined the effect of shin’iseihaito extract (SSHT) on murine allergic rhinitis model us ing ovalbumin (OVA). We decocted the mixture of 9 crude drugs in water to prepare SSHT. SSHT (20 times amount of human dose) was orally administered to mice treated with OVA. After mice were sacrificed on day 28, immunoglobulin (Ig) E, interleukin (IL)-4, IL-13, interferon (IFN)-γ, and thymic strom al lymphopoietin (TSLP) levels in nasal lavage fluid samples were measured by enzyme-linked immunosorbent assay (ELISA). The pathological tissue sections from the nasal epithelial mucosa were histopathologically investigated by optica...
Source: Journal of Natural Medicines - November 18, 2021 Category: Drugs & Pharmacology Source Type: research
Identification of 1 β,2α-epoxytagitinin C as a Notch inhibitor, oxidative stress mechanism and its anti-leukemia activity
AbstractNotch signaling plays crucial roles in cell differentiation and proliferation, but aberrant activation of this signaling results in tumorigenesis and cancer progression. Notch signaling is thus a promising drug target for oncotherapy, and the development of Notch signaling inhibitors is eagerly awaited. Notch inhibitory activity-guided fractionation of aSpilanthes acmella extract led to the identification of five sesquiterpene lactones: tagitinin A (1), 1 β,2α-epoxytagitinin C (2), tagitinin C (3), orizabin (4), and 2 α-hydroxytirotundin (5). 1 β,2α-Epoxytagitinin C (2) exhibited Notch signaling inhibition, wi...
Source: Journal of Natural Medicines - November 15, 2021 Category: Drugs & Pharmacology Source Type: research
Cardamonin inhibits the expression of P-glycoprotein and enhances the anti-proliferation of paclitaxel on SKOV3-Taxol cells
AbstractPaclitaxel is widely used in the first-line treatment of ovarian cancer. Nevertheless, the development of acquired resistance to paclitaxel is a major obstacle for the therapy in clinic. Cardamonin is a novel anticancer chalcone which exhibits a wide range of pharmacological activities. However, the effect of cardamonin on paclitaxel-resistant ovarian cancer cells and its underlying molecular mechanisms are unknown. Here, we revealed whether cardamonin had a resensitivity for paclitaxel and furtherly explored the underlying mechanisms on SKOV3-Taxol cells. Our results showed that cardamonin combined with paclitaxel...
Source: Journal of Natural Medicines - November 9, 2021 Category: Drugs & Pharmacology Source Type: research
Constituents of Morus alba var. multicaulis leaf improve lipid metabolism by activating the AMPK signaling pathway in HepG2 cells
AbstractOne new compound, 3Z-1-O-β-d-glucopyranosyl-3-hexene-1,5-diol (1), together with 26 known isolates (2–27) were obtained from the leaf ofMorus alba var.multicaulis. Among the known compounds,7,11,12,14,15,18,19,23, and24 were firstly obtained from theMorus genus;2–5,8,10,13, and20 were firstly isolated fromM. alba. var.multlcaulis. Meanwhile, the NMR data of20 and23 have been reported here for the first time. Moreover, compounds1–11,13,21, and23–27 showed inhibitory effects on triglyceride (TG) accumulation in HepG2 cells. In mechanism, compound1 could activate the phosphorylation of AMP-activated protein k...
Source: Journal of Natural Medicines - November 6, 2021 Category: Drugs & Pharmacology Source Type: research
Aplydactylonins A-C, three new sesquiterpenes from the Vietnamese sea hare Aplysia dactylomela and their cytotoxicity
AbstractAplydactylonins A-C (1–3), three new sesquiterpenes, were isolated from the Vietnamese sea hareAplysia dactylomela. Their structures and absolute configurations were elucidated based on spectroscopic analysis, X-ray crystallography, and density functional theory (DFT) calculations of NMR and ECD data. Compound2 exhibited cytotoxicity against HepG2, DU145 and A549 cells with respective IC50 values of 4.08 ± 0.63, 38.64 ± 1.04 and 12.33 ± 0.95 µM. In addition, HepG2 cells treated with 5 μM compound2 for 48 h showed a significant increase in early apoptotic cells (P < 0.05) and increase...
Source: Journal of Natural Medicines - November 6, 2021 Category: Drugs & Pharmacology Source Type: research
Magnolol upregulates CHRM1 to attenuate Amyloid- β-triggered neuronal injury through regulating the cAMP/PKA/CREB pathway
AbstractAlzheimer ’s disease (AD) is a common neurodegenerative disease characterized by neuronal degeneration and hyperphosphorylated Tau. Magnolol is an active component isolated fromMagnolia officinalis with potential neuroprotection activity. However, the function and mechanism of magnolol in AD progression is largely uncertain. In present study, the biomarkers related to AD and magnolol were predicted by bioinformatics analyses. The key biomarker levels were predicted by GSE5281 and GSE36980 using AlzData. Cell viability was detected by CCK-8 assay. mRNA and protein levels were examined by qRT-PCR and western blotti...
Source: Journal of Natural Medicines - October 27, 2021 Category: Drugs & Pharmacology Source Type: research
Correction to: Leveraging knowledge of Asian herbal medicine and its active compounds as COVID-19 treatment and prevention
(Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - October 23, 2021 Category: Drugs & Pharmacology Source Type: research
Eleven new C19-diterpenoid alkaloids from Delphinium elatum cv. Pacific Giant
AbstractDiterpenoid alkaloids, the main components of plants of the generaAconitum,Delphinium, andGarrya, are a group of natural products with notable chemical properties and biological activities. Several C19-diterpenoid alkaloid components fromDelphinium elatum cv. Pacific Giant, as well as their derivatives, exhibited cytotoxic activity against lung, prostate, cervical, and vincristine-resistant cervical cancer cell lines. In the current phytochemical investigation on the seeds ofD. elatum cv. Pacific Giant, eleven new C19-diterpenoid alkaloids, elapaciline (1), meladine (2), melapacitine (3), iminoeladine (4), 19-oxopa...
Source: Journal of Natural Medicines - October 20, 2021 Category: Drugs & Pharmacology Source Type: research
Cheminformatics modeling of the correlation between Bupleurum Root-formula medicines and Excess and Deficiency pattern in the diagnostic criteria of Sho in Kampo (traditional Japanese medicine) by non-targeted direct infusion mass spectrometry with machine learning
AbstractKampo is a form of traditional Japanese medicine, and its therapeutic strategy has been validated empirically over millennia, mainly in Asia. Kampo therapy aims to holistically prevent and treat disease based on the specific diagnosisSho (in Japanese), in contrast with modern medical treatment which focuses on a patient ’s affected parts and local conditions. The medicines formulated using crude drugs derived from natural sources (Kampo formulas) are prescribed for patients according to their specificSho, and thus the Kampo medication system is very complex. However, our previous study strongly suggested that Kam...
Source: Journal of Natural Medicines - October 18, 2021 Category: Drugs & Pharmacology Source Type: research
Essential oils from Melia azedarach L. (Meliaceae) leaves: chemical variability upon environmental factors
AbstractThe chemical composition of the essential oils extracted from the leaves ofMelia azedarach L. collected monthly from July 2019 to June 2020 was examined via gas chromatography (GC) and gas chromatography –mass spectrometry (GC–MS) techniques. Analysis of the essential oils identified about 17 compounds representing more than 85% of the oil. Oil yields were higher in the months of June and August, and the primary compounds identified were β-caryophyllene (3.50–63.41%), benzaldehyde (3.50–55. 98%), and azulene (1.27–19.05%). A correlation analysis was performed to determine the relationship between yields ...
Source: Journal of Natural Medicines - October 18, 2021 Category: Drugs & Pharmacology Source Type: research
Correction to: Drug-interaction between paclitaxel and goshajinkigan extract and its constituents
(Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - October 17, 2021 Category: Drugs & Pharmacology Source Type: research
Analgesic potency of intrathecally administered punicalagin in rat neuropathic and inflammatory pain models
We examined whether punicalagin ameliorates neuropathic pain and inflammatory pain in the spinal cord. Male Sprague –Dawley rats were subjected to chronic constriction injury (CCI) of the sciatic nerve, and an intrathecal catheter was implanted for drug administration. The electronic von Frey test and cold-plate test were performed in CCI rats to evaluate mechanical and cold hyperalgesia in neuropathic pain, an d the formalin test was performed in normal rats to evaluate acute and persistent inflammatory pain. An open-field test was conducted to explore whether punicalagin affects locomotor activity in CCI rats. Punicala...
Source: Journal of Natural Medicines - October 13, 2021 Category: Drugs & Pharmacology Source Type: research
Leveraging knowledge of Asian herbal medicine and its active compounds as COVID-19 treatment and prevention
AbstractThe outbreak of COVID-19 disease has led to a search for effective vaccines or drugs. However, insufficient vaccine supplies to meet global demand and no effective approved prescribed drugs for COVID-19 have led some people to consider the use of alternative or complementary medicines, such as traditional herbal medicine. Medicinal plants have various therapeutic properties that depend on the active compounds they contain. Obviously, herbal medicine has had an essential role in treatment and prevention during COVID-19 outbreak, especially in Asian cultures. Hence, we reviewed the uses of herbal medicine in Asian cu...
Source: Journal of Natural Medicines - October 8, 2021 Category: Drugs & Pharmacology Source Type: research
Efficient fertilization in the cultivation of Angelica acutiloba (Siebold & amp; Zucc.) Kitag. in Hokkaido: Effect of amount of supplied nitrogen on growth, yield, and quality of A. acutiloba
In this study, we compared plant growth and harvest yield ofA. acutiloba cultivated with different amounts of nitrogen (N) fertilizer. When plants were fertilized with 24 kg N/10 a, the aerial part was lush, and the diameter at the top of the root was about 1.3 times thicker than that in plants treated with 12 kg N/10 a. On the other hand, the weight of the harvested root grown with 24 kg N/10 a was slightly, although not significantly, less than that in plant s grown with 12 kg N/10 a. In addition, we found that the content of (Z)-ligustilide, a major essential oil contained inA. acutiloba root, is affected by ni...
Source: Journal of Natural Medicines - October 7, 2021 Category: Drugs & Pharmacology Source Type: research
