Constituents of Morus alba var. multicaulis leaf improve lipid metabolism by activating the AMPK signaling pathway in HepG2 cells

AbstractOne new compound, 3Z-1-O-β-d-glucopyranosyl-3-hexene-1,5-diol (1), together with 26 known isolates (2–27) were obtained from the leaf ofMorus alba var.multicaulis. Among the known compounds,7,11,12,14,15,18,19,23, and24 were firstly obtained from theMorus genus;2–5,8,10,13, and20 were firstly isolated fromM. alba. var.multlcaulis. Meanwhile, the NMR data of20 and23 have been reported here for the first time. Moreover, compounds1–11,13,21, and23–27 showed inhibitory effects on triglyceride (TG) accumulation in HepG2 cells. In mechanism, compound1 could activate the phosphorylation of AMP-activated protein kinaseα (AMPKα) to accelerate theβ-oxidation of fatty acids via promoting the phosphorylation of acetyl-CoA carboxylase 1 and up-regulating carnitine palmitoyl-transferase 1A. Besides, compound1 exerted lipolysis effect by activating hormone-sensitive lipase. In brief, compound1 might play a role by up-regulating phosphorylation of AMPKα, enhancing the fatty acidβ-oxidation and lipolysis.Graphical abstract27 compounds were obtained from the leaf ofMorus alba var.multicaulis. Among them, 18 showed inhibitory effects on TG accumulation in HepG2 cells. Moreover, the new compound, 3Z-1-O-β-d-glucopyranosyl-3-hexene-1,5-diol (1), was found to play a role by up-regulating phosphorylation of AMPKα, enhancing the fatty acidsβ-oxidation and lipolysis.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research