Macrolide sesquiterpene pyridine alkaloids from the stems of Tripterygium regelii
AbstractNine new monomacrolide sesquiterpene pyridine alkaloids, macroregelines  A–I (1–9), were isolated from the stems ofTripterygium regelii, along with a known alkaloid, tripfordine  B. The structures of all the isolated compounds were characterized by extensive one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopic analyses, as well as high-resolution electrospray-ionization mass spectrometry (HRESIMS) data. Compounds4,5,6, and8 showed antiproliferative effect on human rheumatoid arthritis synovial cell line MH7A at concentration of 20  μM, reducing t...
Source: Journal of Natural Medicines - August 13, 2018 Category: Drugs & Pharmacology Source Type: research

Seven new resin glycosides from the seeds of Quamoclit  ×  multifida
AbstractSeven new resin glycosides, multifidins III (1) –IX (7), were isolated from the seeds ofQuamoclit × multifida (syn.Q. sloteri House) (Convolvulaceae), along with five known glycosides, quamoclinic acid B methyl ester (8), operculin XIII (9), quamoclin I (10), QM-10 (11), and QM-12 (12). Their structures were determined on the basis of spectroscopic data and chemical evidence. These compounds were of two different types, i.e., those with macrolactone structures and those with non-macrolactone structures. Additionally, cytotoxic activity towards HL-60 human leukemia cells of1,2,5,8,9,11, and...
Source: Journal of Natural Medicines - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

Preparation of menisdaurigenin and related compounds
AbstractMenisdaurin (1), a cyano glucoside, was first isolated in 1978 fromMenispermum dauricum (Menispermaceae) and named after the plant. It has been also isolated from several plant sources. The stereochemistry of the aglycone part was first reported as (Z,4R,6S)-enantiomer of (4,6-dihydroxy-2-cyclohexen-1-ylidene)acetonitrile based on the CD spectrum of menisdaurilide (2), the α,β-unsaturated γ-lactone obtained by an acid hydrolysis of menisdaurin. Later, the absolute stereochemistry was revised as (Z,4S,6R) by X-ray crystal analysis of1 isolated fromSaniculiphyllum guangxiens. The aglycone part of men...
Source: Journal of Natural Medicines - August 9, 2018 Category: Drugs & Pharmacology Source Type: research

Cycloartane triterpenoid (23 R , 24 E )-23-acetoxymangiferonic acid inhibited proliferation and migration in B16-F10 melanoma via MITF downregulation caused by inhibition of both β-catenin and c-Raf–MEK1–ERK signaling axis
In this study, we demonstrated that 23R-AMA inhibited cell proliferation and basic FGF (bFGF)-induced migration in B16-F10 cells. Furthermore, 23R-AMA promoted ser45/thr41 phosphorylation of β-catenin and suppressed its intranuclear accumulation, which was suggested to be related to inhibition of MITF expression. The transcriptional activity of MITF is known to be regulated by phosphorylation via activated ERK. Further investigation revealed that 23R-AMA inhibited phosphorylation of c-Raf, MEK-1, and ERK, and also that of upstream molecules including FAK and c-Src. These results suggested that 23R-AMA inhibited growth...
Source: Journal of Natural Medicines - August 6, 2018 Category: Drugs & Pharmacology Source Type: research

Correction to: Naturally occurring cell adhesion inhibitors
The article Naturally occurring cell adhesion inhibitors, written by Satoshi Takamatsu, was originally published electronically on the publisher ’s internet portal. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - August 3, 2018 Category: Drugs & Pharmacology Source Type: research

Effects of fermented Sorghum bicolor L. Moench extract on inflammation and thickness in a vascular cell and atherosclerotic mice model
In this study, we examined the anti-atherosclerotic effect of s orghum, which is known to have anti-oxidant and anti-inflammatory activity. A 50% ethanol extract ofSorghum bicolor L. Moench fermented withAspergillus oryzae NK (fSBE) was used for experiments. In vitro expression of endothelial adhesion molecules VCAM-1 and ICAM-1 and pro-inflammatory factor cyclooxygenase-2 was significantly decreased and that of the anti-atherogenic factor heme oxygenase-1 significantly increased byfSBE (P  
Source: Journal of Natural Medicines - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

Selective inhibition of P-gp transporter by goniothalamin derivatives sensitizes resistant cancer cells to chemotherapy
In conclusion, our findings revealed a novel role of goniothalamin derivatives in reversing P-gp-mediated chemotherapy resistance. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - July 31, 2018 Category: Drugs & Pharmacology Source Type: research

The analgesic effect and possible mechanisms by which koumine alters type II collagen-induced arthritis in rats
AbstractGelsemium elegans Benth. is a toxic plant that has been used as an ancient Chinese herbal remedy for rheumatoid arthritis (RA) and nervous pain, spasticity, skin ulcers, and cancers. Koumine, one of its representative alkaloids, shows numerous promising pharmacological activities, including anti-inflammatory and analgesic activities. Here, we investigated the analgesic effect of koumine on the collagen-induced arthritis (CIA) rat model of RA and explored the potential pharmacological mechanisms underlying the analgesia. In the CIA rats, repeated koumine treatments significantly reduced pain compared to controls and...
Source: Journal of Natural Medicines - July 27, 2018 Category: Drugs & Pharmacology Source Type: research

Three new C -glycosyflavones with acetyl substitutions from Swertia mileensis
AbstractThree new acetylatedC-glycosylflavones, 3 ″,6″-di-O-acetylswertiajaponin (1), 4 ″,6″-di-O-acetylswertiajaponin (2), and 6 ″-O-acetylswertiajaponin (3), together with six known compounds were isolated from the whole herb ofSwertia mileensis. Their structures were elucidated on extensive NMR experiments and mass spectrometry studies.1H and13C NMR data exhibited doublet signals at room temperature. Variable temperature1H NMR experiments were carried out to investigate the presence of rotational isomerism ofC-glycosylflavones. All compounds showed potential antioxidant activities against a...
Source: Journal of Natural Medicines - July 26, 2018 Category: Drugs & Pharmacology Source Type: research

Two new secoiridoid glucosides and a new lignan from the roots of Ilex pubescens
AbstractTwo new secoiridoid glucosides, ilexpublignoside (1), pubzenoside (2), and a new lignan, ilexlignan B (3), along with seven known compounds (4 –10) were isolated from the roots ofIlex pubescens for the first time. Their chemical structures were elucidated on the basis of extensive spectroscopic methods, including IR, UV, HR-ESI –MS, CD, NMR experiments, as well as comparison with the reported data. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - July 25, 2018 Category: Drugs & Pharmacology Source Type: research

Bioassay-guided isolation of cantharidin from blister beetles and its anticancer activity through inhibition of epidermal growth factor receptor-mediated STAT3 and Akt pathways
In this study, we isolated canthari din from cantharides by bioassay-guided fractionation and examined its inhibitory effect on STAT3 activation in human breast cancer MDA-MB-231 cells, expressing high level of phosphorylated STAT3. Cantharides were extracted with acetonitrile and separated into hexane, methylene chloride/acetonitrile , and water fractions. The methylene chloride/acetonitrile fraction was further separated into four fractions by preparative high-throughput high-performance liquid chromatography. Cantharidin was then isolated from the third fraction by countercurrent chromatography and structurally det...
Source: Journal of Natural Medicines - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway
AbstractOur aim is to investigate the potential therapeutic value of morin against osteoporosis and elucidate the mechanism of action. Osteoporosis was induced in rats by a subcutaneous injection of dexamethasone (DEX) for 5  weeks. Body weight was regularly monitored. Body mineral density (BMD) was determined at proximal femurs using dual energy X-ray absorptiometry. Pathological examination was performed by hematoxylin and eosin staining. The relative expression of osteogenic and bone resorption markers was determine d by real-time polymerase chain reaction and Western blotting, respectively. Activation of the MAPK ...
Source: Journal of Natural Medicines - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Hydroxyobtustyrene protects neuronal cells from chemical hypoxia-induced cell death
AbstractHydroxyobtustyrene is a derivative of cinnamyl phenol isolated fromDalbergia odorifera T. Chen. The heartwood, known as ‘JiangXiang’, is a traditional Chinese medicine. Previous studies showed that hydroxyobtustyrene inhibited the biosynthesis of prostaglandins, which are mediators of neuronal cell death in ischemia. However, it currently remains unclear whether hydroxyobtustyrene protects neurons against ischemi c stress. In the present study, we investigated the protective effects of hydroxyobtustyrene against sodium cyanide (NaCN)-induced chemical ischemia. Hippocampal neurons were cultured from the ...
Source: Journal of Natural Medicines - July 9, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts
In this study, it was revealed that there are two chemotypes (Types A and B) in the root of the original plant,O. japonicus. Methylophiopogonanone A (compound1) and methylophiopogonanone B (compound2) were isolated as index compounds from Type A and compound1 and ophiopogonanone A (compound3) from Type B. In addition, ophiopogonin B (compound4) was isolated as the main steroidal saponin from both Type A and B. The results indicated that two different methanol extracts (from Types A and B) and the main constituents ofO. japonicus (compound1–4), significantly downregulated the expression of interleukin (IL)-6 and IL-8,...
Source: Journal of Natural Medicines - June 30, 2018 Category: Drugs & Pharmacology Source Type: research

Naturally occurring cell adhesion inhibitors
AbstractThis paper reviews naturally occurring cell adhesion inhibitors derived from a plant, microbial and marine origin. Plant-derived inhibitors are classified according to a type of structure. Microbially and marine-derived inhibitors were described according to age. In addition, effects of inhibitors on cell proliferation and that of standards on cell adhesion are listed as much as possible. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - May 19, 2018 Category: Drugs & Pharmacology Source Type: research

Comparison of lawsone contents among Lawsonia inermis plant parts and neurite outgrowth accelerators from branches
In this study, we measured lawsone contents in the extracts ofL. inermis flowers, leaves and branches by HPLC with tandem mass spectrometry. The extracts ofL. inermis flowers, leaves and branches contained 116.9, 486.2 and 5.4  μg/g lawsone, respectively. Lawsone content was much lower in branches than the other plant parts. Next, in order to identify the biological constituents in the branches, we isolated nine known compounds and examined their effects on neurite outgrowth in PC12 cells. Among the constituents isolate d, 2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-propane-1,3-diol (1) and quercetin 7-O- β-D-glu...
Source: Journal of Natural Medicines - May 17, 2018 Category: Drugs & Pharmacology Source Type: research

Genistein has the function of alleviating and treating disseminated intravascular coagulation caused by lipopolysaccharide
AbstractSymptoms of disseminated intravascular coagulation (DIC) include thromboembolism, acute attrition bleeding and multiple organ failure. Genistein isolated from leguminous plants has been shown to be effective in oxidation resistance and tumor inhibition. The present study was designed to evaluate the therapeutic effects of genistein in DIC and preliminarily discuss the mechanisms regarding the anti-inflammatory and anticoagulant effect of genistein. Swiss mice were randomly divided into the following groups —(1) lipopolysaccharide (LPS), (2) genistein, (3) dimethyl sulfoxide (DMSO, the non-major solvent compon...
Source: Journal of Natural Medicines - May 14, 2018 Category: Drugs & Pharmacology Source Type: research

New podolactones from the seeds of Podocarpus nagi and their anti-inflammatory effect
In this study, the seeds ofP. nagi were isolated by comprehensive chromatographic methods to obtain three new podolatones, named nagilactone B 1-O- β-d-glucoside (1), nagilactone N3 3-O- β-d-glucoside (2), and 2-epinagilactone B (3), as well as a known compound, nagilactone B (4). Their structures were determined by analyses of NMR and HRESIMS data. Compounds1 and2 significantly inhibited nitric oxide (NO) production on LPS-stimulated RAW264.7 macrophages, with IC50 values of 0.18  ± 0.04 and 0.53 ± 0.03 μM, respectively. Indomethacin (IC50 4.21  ±&t...
Source: Journal of Natural Medicines - May 11, 2018 Category: Drugs & Pharmacology Source Type: research

Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis
AbstractThe protective potential of dandelion on acute hepatitis, lung injury and colorectal cancer has recently been revealed. Importantly, ulcerative colitis (UC), a clinically defined inflammatory bowel disease, accelerates the risk of colorectal cancer. However, studies focusing on the activity of dandelion on UC are extremely limited. In the present study, we found that an aqueous extract of dandelion root increases cell viability and decreases apoptosis in dextran sodium sulfate (DSS)-incubated NCM460 human colonic epithelial cells, probably through removing the production of reaction oxygen species and blocking nucl...
Source: Journal of Natural Medicines - May 8, 2018 Category: Drugs & Pharmacology Source Type: research

Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms
This study is the first report on comparative transcriptome analyses of medicinally importantForsythia genus and will serve as an important resource to facilitate further studies on biosynthesis and regulation of therapeutic compounds inForsythia species. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - May 7, 2018 Category: Drugs & Pharmacology Source Type: research

Greetings from the chief editor
(Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - April 26, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-inflammatory and anti-angiogenic activities in vitro of eight diterpenes from Daphne genkwa based on hierarchical cluster and principal component analysis
In conclusion, eight diterpenes isolated fromD. genkwa showed different levels of activity in LPS-induced RAW264.7 cells and TNF- α-stimulated HUVECs. The comprehensive evaluation of activity by HCA and PCA indicated that of the eight diterpenes, 12-O-(2 ′E,4′E-decadienoyl)-4-hydroxyphorbol-13-acetyl was the best, and can be developed as a new drug for RA therapy. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - April 21, 2018 Category: Drugs & Pharmacology Source Type: research

Berberine protects renal tubular cells against hypoxia/reoxygenation injury via the Sirt1/p53 pathway
AbstractBerberine (BBR) has been demonstrated to protect against renal ischemia/reperfusion injury; however, the underlying molecular mechanism is largely unknown. In the present study, we examined the role of silent information regulator 1 (Sirt1)/p53 in the protective effect of BBR on hypoxia/reoxygenation (H/R)-mediated mitochondrial dysfunction in rat renal tubular epithelial cells (NRK-52E cells). NRK-52E cells were preconditioned with small interfering RNA targeting Sirt1 (Sirt1-siRNA)  and BBR before subjected to H/R. Cell damage was assessed by CCK8 assay and detection of oxidative parameters. The apoptotic ra...
Source: Journal of Natural Medicines - April 21, 2018 Category: Drugs & Pharmacology Source Type: research

Changes in content of triterpenoids and polysaccharides in Ganoderma lingzhi at different growth stages
In this study, we quantified 18 triterpenoids, total triterpenoid content and total polysaccharide content in the ethanol and water extracts ofG. lingzhi at different growth stages. Triterpenoids were quantified by liquid chromatograph –tandem mass spectrometry in the multiple-reaction-monitoring mode. Total triterpenoid and total polysaccharide content were determined by colorimetric analysis. The results indicated that the fruit bodies at an early growth stage had a higher content of ganoderic acid A, C2, I and LM2, as well as of ganoderenic acid C and D, than those at a later growth stage. In contrast, ganoderic a...
Source: Journal of Natural Medicines - April 20, 2018 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of the anti-inflammatory activity of orengedokuto II: berberine is responsible for the inhibition of NO production
We report here our analysis of the anti-inflammatory effect of the component crude drugs of orengedokuto and their constituents using the inhibition of nitric oxide (N O) production in the murine macrophage-like cell line J774.1. An initial comparison of NO production inhibitory activities of the extracts of the component crude drugs and their combinations revealed that the activity could be attributed to Phellodendron Bark and Coptis Rhizome. Berberine (1), the major constituent of these crude drugs, showed potent activity (IC50 4.73  ± 1.46 μM). Quantitative analysis of1 in the extracts of al...
Source: Journal of Natural Medicines - April 18, 2018 Category: Drugs & Pharmacology Source Type: research

Impact of different post-harvest processing methods on the chemical compositions of peony root
AbstractThe impact of key processing steps such as boiling, peeling, drying and storing on chemical compositions and morphologic features of the produced peony root was investigated in detail by applying 15 processing methods to fresh roots ofPaeonia lactiflora and then monitoring contents of eight main components, as well as internal root color. The results showed that low temperature (4  °C) storage of fresh roots for approximately 1 month after harvest resulted in slightly increased and stable content of paeoniflorin, which might be due to suppression of enzymatic degradation. This storage also prevented r...
Source: Journal of Natural Medicines - April 13, 2018 Category: Drugs & Pharmacology Source Type: research

Mitogenic activity of Artocarpus lingnanensis lectin and its apoptosis induction in Jurkat T cells
AbstractLectins are a class of carbohydrate-binding proteins or glycoproteins and used in the purification and characterization of glycoproteins according to their specificity to carbohydrates. In the present study, the mitogenic activity ofArtocarpus lingnanensis lectin (ALL) and its apoptosis induction in Jurkat T cells were explored. MTT assay revealed strong mitogenic potential of ALL. Meanwhile, the anti-cancer activity of ALL was also explored using the human leukemic Jurkat T cell line. ALL exhibited strong binding affinity (97%) to the cell membrane, which could be effectively inhibited byN-acetyl-d-galactosaminide...
Source: Journal of Natural Medicines - April 12, 2018 Category: Drugs & Pharmacology Source Type: research

Toosendanin mediates cisplatin sensitization through targeting Annexin A4/ATP7A in non-small cell lung cancer cells
AbstractCisplatin (CDDP) is used in the treatment of non-small cell lung cancer (NSCLC), but due to the development of resistance, the benefit has been limited. Toosendanin (TSN) has shown therapeutic effects on NSCLC; however, the role of TSN on CDDP sensitization in NSCLC remains unknown. The antitumor effects of TSN and CDDP sensitization mediated by TSN were explored. TSN was added in various amounts to measure dose- and time-dependent cytotoxicity. Intracellular CDDP was detected by high-performance liquid chromatography. The protein levels of ATP7A, ATP7B, hCTR1, MRP-2, P-gp and Annexin A4 (Anxa4) were analyzed. The ...
Source: Journal of Natural Medicines - April 7, 2018 Category: Drugs & Pharmacology Source Type: research

Chemical constituents from Canarium album Raeusch and their anti-influenza A virus activities
AbstractTwo new dyhydrophaseic acid glucoside isomers, (1 ′S, 3 ′R, 5 ′S, 8 ′R, 2Z, 4E)-dihydrophaseic acid-3 ′-O-β-d-glucopyranoside (2) and (1 ′R, 3 ′S, 5 ′R, 8 ′R, 2Z, 4E)-dihydrophaseic acid-3 ′-O-β-d-glucopyranoside (4), together with 10 known compounds [myo-inositol (1), 3,4-dihydroxybenzoic acid (3), 3-O-galloyl quinic acid (5), ellagic acid (6), gallic acid (7), ethyl gallate (8), scopoletin (9), ellagic acid-4-O-β-d-glucopyranoside (10), ellagic acid-4-O-α-l-rhamnopyranoside (11), and isocorilagin (12)] were isolated from the chlorofo...
Source: Journal of Natural Medicines - April 5, 2018 Category: Drugs & Pharmacology Source Type: research

Appetite-enhancing effects of vanilla flavours such as vanillin
AbstractVanilla flavour is familiar to consumers through foods, cosmetics, household products and some medicines. Vanilla flavouring agents typically contain vanillin or its analogue ethyl vanillin. Our previous study revealed that the inhalation of eugenol, which contains a vanillyl group, has an appetite-enhancing effect, and the inhalation of aroma compounds containing the vanillyl group or its analogues led to increased food intake in mice. Here, we found that vanillin, ethyl vanillin and eugenol showed appetite-enhancing effects, whereas isoeugenol and safrole did not. These results suggest that the appetite-enhancing...
Source: Journal of Natural Medicines - March 22, 2018 Category: Drugs & Pharmacology Source Type: research

Cellular mechanism for herbal medicine Junchoto to facilitate intestinal Cl − /water secretion that involves cAMP-dependent activation of CFTR
AbstractConstipation is a common symptom frequently compromising the quality of daily life. Several mechanistically different drugs have been used to mitigate constipation, including Japanese herbal (Kampo) medicines. However, the mechanisms of their actions are often not well understood. Here we aimed to investigate the molecular mechanisms underlying the effects of Junchoto (JCT), a Kampo medicine empirically prescribed for chronic constipation. Cl− channel activity was measured by the patch-clamp method in human cystic fibrosis transmembrane conductance regulator (CFTR)-expressing HEK293T cells and human intestina...
Source: Journal of Natural Medicines - March 22, 2018 Category: Drugs & Pharmacology Source Type: research

Quantification of terpene trilactones in Ginkgo biloba with a 1 H NMR method
AbstractGinkgo biloba L. has been used as a herbal medicine in the traditional treatment of insufficient blood flow, memory deficits, and cerebral insufficiency. The terpene trilactone components, the bioactive agents ofGinkgo biloba L., have also been reported to exhibit useful functionality such as anti-inflammatory and neuroprotective effects. Therefore, in the present research, we attempted to analyze quantitatively the terpene trilactone components inGinkgo biloba leaf extract, with quantitative1H  NMR (qNMR) and obtained almost identical results to data reported using HPLC. Application of the qNMR method for the...
Source: Journal of Natural Medicines - March 22, 2018 Category: Drugs & Pharmacology Source Type: research

Two new pyrrolo-2-aminoimidazoles from a Myanmarese marine sponge, Clathria prolifera
AbstractMarine organisms such as marine sponges and soft corals are valuable sources of pharmacologically active secondary metabolites. In our ongoing research on the discovery of new secondary metabolites from marine organisms, two new pyrrolo-2-aminoimidazoles, clathriroles A (1) and B (2), were isolated from the water-soluble portion prepared from the methanol and acetone (2:1) extract of the marine sponge,Clathria prolifera, collected in Myanmar. The chemical structures of the isolated compounds were determined using extensive spectroscopic techniques, including NMR, HRESIMS, IR, and optical rotation, and comparisons w...
Source: Journal of Natural Medicines - March 22, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-invasive effect of Cyclamen pseudibericum extract on A549 non-small cell lung carcinoma cells via inhibition of ZEB1 mediated by miR-200c
In this study, we investigated the effects of endemicCyclamen pseudibericum (CP) saponin-rich tuber extract on the capacity of non-small cell lung cancer line A549 cells to proliferate, invade and migrate and also examined the expression levels of several invasion –migration-related microRNAs (miRNAs) to identify those which directly targeted ZEB1. The cytotoxicity effect of the CP extract on the A549 cancer cells was determined by the luminometric method. The half-minimal (50%) inhibitory concentration dose in the A549 cells was determined to be 41.64 ±  2.35 µg/mL. Using the Matrigel...
Source: Journal of Natural Medicines - March 19, 2018 Category: Drugs & Pharmacology Source Type: research

Effect of the active ingredient of Kaempferia parviflora , 5,7-dimethoxyflavone, on the pharmacokinetics of midazolam
In this study, its safety was evaluated from a pharmacokinetic point of view, based on daily ingestion of 5,7-DMF. Midazolam, a substrate of CYP3As, was orally administered to mice treated with 5,7-DMF for 10  days, and its pharmacokinetic properties were investigated. In the group administered 5,7-DMF, the area under the curve (AUC) of midazolam increased by 130% and its biological half-life was extended by approximately 100 min compared to the control group. Compared to the control group, 5,7-DMF mar kedly decreased the expression of CYP3A11 and CYP3A25 in the liver. These results suggest that continued ingesti...
Source: Journal of Natural Medicines - March 17, 2018 Category: Drugs & Pharmacology Source Type: research

β-amyloid cytotoxicity is prevented by natural achillolide A
AbstractAlzheimer ’s disease (AD) is the most prevalent cause of dementia in adults. Current available drugs for AD transiently alleviate some of the symptoms, but do not modify the disease mechanism or cure it. Therefore, new drugs are desperately needed. Key contributors to AD are amyloid beta (Aβ)- and reactive oxygen species (ROS)-induced cytotoxicities. Plant-derived substances have been shown to affect various potential targets in various diseases including AD. Therefore, phytochemicals which can protect neuronal cells against these insults might help in preventing and treating this disease. In the fol low...
Source: Journal of Natural Medicines - March 15, 2018 Category: Drugs & Pharmacology Source Type: research

Scutellaria baicalensis regulates FFA metabolism to ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway
This study aimed to explore the effects and mechanisms ofS. baicalensis and its major constituent baicalin on hepatic lipotoxicity. KK-Ay mice and orotic acid (OA)-induced nonalcoholic fatty liver disease (NAFLD) rats were used to evaluate lipid metabolism regulatory effects. Sodium oleate-induced triglyceride-accumulated HepG2 cells were used for the mechanism study, pretreated with or without compound C or STO-609 or transfected with liver kinase B1 (LKB1) siRNA. In KK-Ay mice,S. baicalensis extract showed a decreased effect on serum and hepatic triglycerides, total cholesterols, and free fatty acid (FFA) levels after 8 ...
Source: Journal of Natural Medicines - March 14, 2018 Category: Drugs & Pharmacology Source Type: research

Correction to: Cypripedin, a phenanthrenequinone from Dendrobium densiflorum , sensitizes non-small cell lung cancer H460 cells to cisplatin-mediated apoptosis
In original publication of the article, unfortunately one of the author names was published incorrectly as “Boonchu Sritularuk”. The correct name is “Boonchoo Sritularak”. (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - March 13, 2018 Category: Drugs & Pharmacology Source Type: research

Three new 5,6,7,8-tetrahydroxy-5,6,7,8-tetrahydrochromone derivatives enantiomeric to agarotetrol from agarwood
AbstractAgarwood (jinkoh in Japanese) is a resinous wood fromAquilaria species of the family Thymelaeaceae and has been used as incense and in traditional medicines. Characteristic chromone derivatives such as agarotetrol have been isolated from agarwood. In previous study, we isolated two new 2-(2-phenylethyl)chromones together with six known compounds from MeOH extract of agarwood. Further chemical investigation of the MeOH extract led to isolation of eighteen 2-(2-phenylethyl)chromones, including three new 5,6,7,8-tetrahydroxy-5,6,7,8-tetrahydrochromones with stereochemistry enantiomeric to agarotetrol-type, viz. (5R,6S...
Source: Journal of Natural Medicines - March 10, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-amyloidgenic and neurotrophic effects of tetrahydroxystilbene glucoside on a chronic mitochondrial dysfunction rat model induced by sodium azide
AbstractAlzheimer ’s disease (AD) is an irreversible neurodegenerative brain disorder with complex pathogenesis. Emerging evidence indicates that there is a tight relationship between mitochondrial dysfunction and β-amyloid (Aβ) formation. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is one of the mai n active components extracted fromPolygonum multiflorum. The purpose of the present study was to investigate the effects of TSG on A β production and neurotrophins in the brains of rats by using a mitochondrial dysfunction rat model induced by sodium azide (NaN3), an inhibitor of mitoc...
Source: Journal of Natural Medicines - March 5, 2018 Category: Drugs & Pharmacology Source Type: research

Total syntheses of schizandriside, saracoside and ( ±)-isolariciresinol with antioxidant activities
AbstractLignans are widely distributed in plants and exhibit significant pharmacological effects, including anti-tumor and antioxidative activities. Here, we describe the total synthesis of schizandriside (1), a compound we previously isolated fromSaraca asoca by monitoring antioxidative activity using the 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. Starting from a tandem Michael-aldol reaction, the lignan skeleton was synthesized in 6 steps, including a cyclization step. To determine the stereochemistry of1, we synthesized the natural product ( ±)-isolariciresinol (18) from alcohol17. Comparison of the ...
Source: Journal of Natural Medicines - March 5, 2018 Category: Drugs & Pharmacology Source Type: research

Four new resin glycosides, murasakimasarins I –IV, from the tuber of Ipomoea batatas
AbstractFour new resin glycosides having macrolactone structures (jalapins), murasakimasarins I –IV, were isolated from the tubers ofIpomoea batatas (L.) Lam. ( ‘Murasakimasari’, Convolvulaceae), along with three known glycosides. Their structures were determined on the basis of spectroscopic data as well as chemical evidence. Murasakimasarin III is the first representative of a resin glycoside with 10-methylundecanoic acid as the component organic acid . (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - March 5, 2018 Category: Drugs & Pharmacology Source Type: research

Cladosporamide A, a new protein tyrosine phosphatase 1B inhibitor, produced by an Indonesian marine sponge-derived Cladosporium sp.
AbstractCladosporamide A (1), a new protein tyrosine phosphatase (PTP) 1B inhibitor, was isolated together with a known prenylated flavanone derivative (2) from the culture broth of an Indonesian marine sponge-derivedCladosporium sp. TPU1507 by solvent extraction, ODS column chromatography, and preparative HPLC (ODS). The structure of1 was elucidated based on 1D and 2D NMR data. Compound1 modestly inhibited PTP1B and T-cell PTP (TCPTP) activities with IC50 values of 48 and 54  μM, respectively. The inhibitory activity of2 against PTP1B (IC50 = 11 μM) was approximately 2-fold stronger than that aga...
Source: Journal of Natural Medicines - March 5, 2018 Category: Drugs & Pharmacology Source Type: research

Correction to: Comparison of glycyrrhizin content in 25 major kinds of Kampo extracts containing Glycyrrhizae Radix used clinically in Japan
The article Comparison of glycyrrhizin content in 25 major kinds of Kampo extracts containing Glycyrrhizae Radix used clinically in Japan, written by Mitsuhiko Nose, Momoka Tada, Rika Kojima, Kumiko Nagata, Shinsuke Hisaka, Sayaka Masada, Masato Homma and Takashi Hakamatsuka, was originally published Online First without open access. After publication in volume 71, issue 4, page 711 –722 the author decided to opt for Open Choice and to make the article an open access publication. Therefore, the copyright of the article has been changed to © The Author(s) 2018 and the article is forthwith distributed under the te...
Source: Journal of Natural Medicines - March 1, 2018 Category: Drugs & Pharmacology Source Type: research

Correction to: Ephedra Herb extract activates/desensitizes transient receptor potential vanilloid 1 and reduces capsaicin-induced pain
The article Ephedra Herb extract activates/desensitizes transient receptor potential vanilloid 1 and reduces capsaicin-induced pain, written by Shunsuke Nakamori, Jun Takahashi, Sumiko Hyuga, Toshiko Tanaka-Kagawa, Hideto Jinno, Masashi Hyuga, Takashi Hakamatsuka, Hiroshi Odaguchi, Yukihiro Goda, Toshihiko Hanawa and Yoshinori Kobayashi, was originally published Online First without open access. After publication in volume 71, issue 1, page 105 –113 the author decided to opt for Open Choice and to make the article an open access publication. Therefore, the copyright of the article has been changed to © The Autho...
Source: Journal of Natural Medicines - March 1, 2018 Category: Drugs & Pharmacology Source Type: research

Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei , a Thai herb for menopause remedy
AbstractMiroestrol and deoxymiroestrol are the most potent phytoestrogens ofPueraria candollei var.mirifica, having been proved as an effective herb for menopausal symptoms in folk medicines and clinical trials. To ensure efficacy and safety ofP. candollei var.mirifica involved in nutraceutical products being available worldwide, the content of potent phytoestrogens as active ingredients should be specified. Therefore, in this study, we produced a monoclonal antibody for total analysis of potent estrogenic miroestrol and deoxymiroestrol, for which an analytical method was developed using a procedure for an indirect competi...
Source: Journal of Natural Medicines - February 28, 2018 Category: Drugs & Pharmacology Source Type: research

A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells
This study aimed to evaluate the anticancer activity and related mechanisms of 4,5,4 ′-trihydroxy-3,3′-dimethoxybibenzyl (TDB), a bibenzyl extracted fromDendrobium ellipsophyllum Tang and Wang, in human lung cancer cells. Cytotoxicity of TDB (0 –300 µM) in different types of human lung cancer cells (H460, H292 and H23) and human dermal papilla cells (DPCs) was evaluated via MTT viability assay. Selective anticancer activity of TDB against human lung cancer cells was demonstrated with a high IC50 (approximately  >  300 µM) in DPCs, while IC50 in human lung cancer H460, H2...
Source: Journal of Natural Medicines - February 27, 2018 Category: Drugs & Pharmacology Source Type: research

Two flavonol glycosides from Liparis bootanensis
AbstractTwo new flavonol glycosides, bootanenside  I and II (1 and2), along with ten known compounds (3–12), were isolated from whole plant ofLiparis bootanensis Griff. Their structures were elucidated on the basis of extensive spectroscopic analyses, including high-resolution electrospray-ionization mass spectrometry (HR –ESIMS) and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR). The cytotoxicity of the compounds was investigated against HCT116 human cancer cell line, revealing that none of them possessed considerable cytotoxic activity. Bioassays of the new metabolites sho...
Source: Journal of Natural Medicines - February 26, 2018 Category: Drugs & Pharmacology Source Type: research

New geranyl flavonoids from the leaves of Artocarpus communis
AbstractFour new geranyl flavonoids1–4 and four known flavonoids5–8 were obtained from the leaves ofArtocarpus communis collected in Indonesia. The planar structures of flavonoids were elucidated by analyses of MS and NMR spectroscopic data. Absolute configurations of1 and2 were determined by ECD spectroscopy. Analyses by HPLC with a chiral-phase column and ECD spectra confirmed that3 and4 were stereoisomeric mixtures and7 and8 were racemic mixtures. The compounds obtained in this study inhibited the enzymatic activities of ubiquitin-specific protease 7 (USP7) and the chymotrypsin-like activity of the proteasom...
Source: Journal of Natural Medicines - February 24, 2018 Category: Drugs & Pharmacology Source Type: research

Allergy-preventive effects of linarinic acid and its tetrahydropyrrolo[2,1-b]quinazoline derivatives isolated from Linaria vulgaris
We examined their allergy-preventive effects using an in vivo assay system we developed previously, that monitors a decrease in blood flow in the tail vein of mice subjected to sensitization with hen egg-white lysozyme. We observed that4a and its three derivatives, amide (6a), ester (5a), bromine (4b), and alcohol substituent (6b), showed significant allergy-preventive activities. The study confirmed the allergy-preventive activity of tetrahydropyrrolo[2,1-b]quinazoline derivatives by comprehensively monitoring the specific blood flow decrease occurring in the induction phase of allergy. This finding may aid in the develop...
Source: Journal of Natural Medicines - February 21, 2018 Category: Drugs & Pharmacology Source Type: research