Relationship between Human β-defensin 2 and the vaginal environment.
Relationship between Human β-defensin 2 and the vaginal environment. Jpn J Infect Dis. 2019 Dec 25;: Authors: Kotani H, Koshizuka T, Matsubara K, Nishiyama K, Sugiyama T, Suzutani T Abstract As one of the main antimicrobial peptides, human β-defensin 2 (HBD2) plays multiple roles in the lower genital tract. Based on the Nugent score as a diagnostic criterion for bacterial vaginosis, we sought to clarify the correlations among the Nugent score and Interleukin-6 (IL-6) and HBD2 levels in vaginal secretions in association with various types of infection. Ninety-eight women were recruited for th...
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Kotani H, Koshizuka T, Matsubara K, Nishiyama K, Sugiyama T, Suzutani T Tags: Jpn J Infect Dis Source Type: research

Antimicrobial Resistance Profile and Molecular Characteristics of Staphylococcus aureus Isolates from Hospitalized Adults in Three Regions of Vietnam.
This study aimed to investigate the antimicrobial resistance profile, multidrug resistance (MDR), and molecular characteristics of pathogenic Staphylococcus aureus isolates among hospitalized Vietnamese adults. Two-hundred twenty-three pathogenic S. aureus isolates were collected from hospitals in three regions of Vietnam. The minimum inhibitory concentrations were determined for antibiotic susceptibility of isolates. The molecular characteristics of S. aureus isolates were investigated using analysis of antibiotic-resistant genes, staphylococcal cassette chromosome mec typing, pulsed-field gel electrophoresis, and multilo...
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Son NT, Huong VTT, Lien VTK, Nga DTQ, Au TTH, Hang PTT, Minh HTN, Binh TQ Tags: Jpn J Infect Dis Source Type: research

In vitro ravuconazole susceptibility of anthropophilic dermatophyte strains from Japanese patients.
Abstract Ravuconazole (RVCZ) is a new available human anti-fungal azole drug in Japan since 2018 and broad spectrum antifungal agent that exhibits excellent activity against dermatophytes. In the present study, the in vitro RVCZ susceptibility of clinical isolates for anthropophilic dermatophytes including the itraconazole (ITCZ) low susceptible and the terbinafin (TEBR) resistance strains of T. interdigitale, was investigated using the Clinical & Laboratory Standards Institute (CLSI) M38-A2 test. The MICs for the 20 clinical isolates of T. interdigitale were
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Kano R, Hiruma J, Yokota M, Noguchi H, Hase M, Kamata H, Harada K, Hiruma M Tags: Jpn J Infect Dis Source Type: research

Rat-bite fever due to Streptobacillus moniliformis without bite history: An unexpected cause of consciousness disturbance.
PMID: 31875605 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Onodera H, Uekita H, Watanabe T, Taira K, Watanabe C, Saito H, Seto J, Suzuki Y, Imaoka K Tags: Jpn J Infect Dis Source Type: research

Investigation of Orthohantavirus Seroprevalence in Northern Rural Areas of Denizli Province, Turkey.
This study has the feature of the first publication is reported PUUV seropositivities from the southwestern part of Turkey. PMID: 31875606 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Polat C, Ergin Ç, Akkaya Y, Oktem IMA Tags: Jpn J Infect Dis Source Type: research

Spontaneous non-traumatic Clostridium perfringens sepsis.
Abstract Clostridium species are gram-positive, spore-forming, anaerobic rods normally found in the soil and the gastrointestinal tract of humans and animals. Spontaneous sepsis due to C. perfringens is not caused by injury, which sets it apart from classic gas gangrene that typically follows trauma. Spontaneous C. perfringens sepsis often develops as a rapidly progressive intravascular hemolysis and metabolic acidosis, with high mortality rates of over 70% with standard intensive care. In such cases, alpha-toxin secreted by C. perfringens is considered as the main toxin responsible for intravascular hemolysis, di...
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Hifumi T Tags: Jpn J Infect Dis Source Type: research

An ultra-rapid real-time RT-PCR method for detecting Middle East respiratory syndrome coronavirus using a mobile PCR device, PCR1100.
Abstract Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is usually diagnosed through highly sensitive and specific genetic tests such as real-time reverse transcription polymerase chain reaction (RT-PCR). Currently, two real-time RT-PCR assays targeting the upE and ORF1a regions of the MERS-CoV genome are widely used and are the standard assays recommended by the World Health Organization (WHO). The MERS outbreaks to date suggest that rapid diagnosis and subsequent isolation of infected patients, particularly superspreaders, are critical for containment. However, conventional real-time RT-PCR assay...
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Shirato K, Nao N, Matsuyama S, Kageyama T Tags: Jpn J Infect Dis Source Type: research

Effect of high blood glucose level on the antimicrobial activity of daptomycin against Staphylococcus aureus in streptozotocin-induced diabetic mice.
In conclusion, daptomycin is an alternative therapeutic option in diabetic mice with serious staphylococcal infections regardless of blood glucose control in this animal model. PMID: 31875609 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 25, 2019 Category: Infectious Diseases Authors: Kondo T, Hagihara M, Esaka Y, Yamamoto T, Uno B, Yamagishi Y, Mikamo H Tags: Jpn J Infect Dis Source Type: research

Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase.
In conclusion, the combination of multiplex PCR primers and QIAGEN Multiplex PCR Plus Kit was used to determine the best performance for the rapid and efficient screening of carbapenemase genes in Enterobacteriaceae. The assay had an overall sensitivity of 100% and a specificity of 100%. This PCR assay will compensate for the limitations of phenotypic testing, such as antimicrobial susceptibility testing and the modified carbapenem inactivation method, among others, in clinical and public health settings. PMID: 31787735 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Watahiki M, Kawahara R, Suzuki M, Aoki M, Uchida K, Matsumoto Y, Kumagai Y, Noda M, Masuda K, Fukuda C, Harada S, Senba K, Suzuki M, Matsui M, Suzuki S, Shibayama K, Shinomiya H Tags: Jpn J Infect Dis Source Type: research

Rotavirus vaccine effectiveness against the risk of hospitalization and the impact of public expense for the vaccine on a regional rotavirus gastroenteritis epidemic in Japan.
This study examined the RV vaccine effectiveness against risk of hospitalization based on a retrospective cohort study and the impact of public expense for the RV vaccine on this regional RV gastroenteritis epidemic. The vaccination coverage was significantly higher in children in Shari and Koshimizu than in Abashiri (87.8% vs. 42.7%, respectively, p
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Sato Y, Yamaki Y, Sasaki Y, Kajino H Tags: Jpn J Infect Dis Source Type: research

Detection of a quinolone-resistant mutation of gyrA in Escherichia albertii.
PMID: 31787737 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Konno T, Kashio H, Takahashi S, Suzuki S, Kumagai Y Tags: Jpn J Infect Dis Source Type: research

Human heavy chain antibody genes elicited in Thai dengue patients during DENV2 secondary infection.
Abstract Dengue is one of the most serious mosquito-borne viral diseases occurring in humans. From their complexity of 4 serotypes, the ideal vaccine for dengue should be able to stimulate cross-neutralizing antibodies. Recently, genetic-based immune response have been studied for guiding of vaccine design for several viral pathogens. Despite a recent approval of dengue vaccine, the information of genetics-based immune response against dengue virus (DENV) are still limited. Consequently, we aimed to determine the profiles of immunoglobulin heavy chain gene from DENV2 infected patients. The immunoglobulin heavy cha...
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Thammasonthijarern N, Puangmanee W, Sriburin P, Injampa S, Chatchen S, Phumirattanaprapin W, Pipattanaboon C, Ramasoota P, Pitaksajjakul P Tags: Jpn J Infect Dis Source Type: research

Pathogens responsible for early-onset sepsis in Suzhou, China.
Abstract Early onset sepsis (EOS) in neonates is a serious disease with severe complications. The increased severity of EOS and risk of death for newborns in recent years means that continued monitoring to detect possible changes in pathogen etiology, disease severity and disease outcome is particularly important. We performed a retrospective study on early-onset infection among infants (birth weights>800 g) who were hospitalized in the Children's Hospital of Soochow University from Jan1, 2011 to Dec 31, 2017. Multivariable analysis was conducted to determine the significant predictors of mortality. The most fr...
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Lu L, Li P, Pan T, Feng X Tags: Jpn J Infect Dis Source Type: research

Genotyping of the human papillomavirus in cervical squamous intraepithelial lesions in Mexican women.
This study aimed to identify the HPV by genotype in SILs using a linear array genotyping test in a population in the State of México. We performed a cross-sectional study of 129 patients (women of the State of México) who completed a risk factor questionnaire and were diagnosed as being with or without SIL by colposcopy. We obtained cervical swab samples from these patients and genotyped them using a Linear Array HPV Genotyping assay from ROCHE®. Forty-nine (37.98%) samples were positive for the HPV, and 24 genotypes were found among these samples. The most common genotype was HPV-16. The 12 genotypes fou...
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Colín-Ferreyra MDC, Domínguez-García MV, Mendieta-Zerón H, Camarillo-Romero MDS, Pérez-Rogel M, Martínez-Madrigal M, Martínez-Pérez S, Romero-Figueroa MDS Tags: Jpn J Infect Dis Source Type: research

Detection of Oropouche virus in saliva and urine samples of febrile patients in Salvador, Bahia, Brazil.
This report shows the need for an efficient surveillance system to control the spread of this virus in the population and suggests the use of saliva and urine samples as an alternative for the detection of OROV when serum samples are not available. PMID: 31787741 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Fonseca LMDS, Carvalho RH, Bandeira AC, Sardi SI, Campos GS Tags: Jpn J Infect Dis Source Type: research

Functional analysis of trichodysplasia spinulosa-associated polyomavirus-encoded large T antigen.
Abstract Trichodysplasia spinulosa-associated polyomavirus (TSPyV or human polyomavirus 8) was identified from patients with trichodysplasia spinulosa, a rare skin disease affecting the faces of immunocompromised patients. Like other polyomaviruses, the TSPyV genome encodes a large T antigen (LT). However, the expression and functions of TSPyV LT in infected cells remain largely unknown. In the present study, we cloned a full-length of TSPyV LT cDNA from cells transfected with full-length of TSPyV LT DNA. Transfection study using green fluorescence protein-tagged LT expression plasmids showed that TSPyV LT was exp...
Source: Japanese Journal of Infectious Diseases - November 29, 2019 Category: Infectious Diseases Authors: Nako T, Fukumoto H, Hasegawa H, Saeki H, Katano H Tags: Jpn J Infect Dis Source Type: research

Prevalence of MDR salmonellae with increasing frequency of S. Kentucky and S. Virchow serovars among hospitalized diarrheal cases in and around Delhi, India.
Abstract Non-typhoidal salmonellae (NTS) are a major cause of acute diarrhea with characteristic multidrug resistance. In a hospital based study, 81 NTS were isolated and tested for serotypes and antimicrobial resistance (AMR). Salmonella enterica isolates were classified into 7 different typable serovars and 19 (23%) isolates remained untypable. The most common serovars were S. Kentucky (48%), and S. Virchow (22%). Most of the NTS isolates displayed resistance to nalidixic acid (73%), ciprofloxacin (48%), ampicillin and norfloxacin (36% each) and gentamicin (31%). The AMR profiles for gentamicin and nalidixic aci...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Sharma NC, Kumar D, Sarkar A, Chowdhury G, Mukhopadhyay AK, Ramamurthy T Tags: Jpn J Infect Dis Source Type: research

Development of a universal and lineage-specific primer sets for Zika virus (ZIKV) rapid detection in blood and urine samples by using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP).
In this study, a universal LAMP primer set was developed to target conserved sequence of known ZIKV lineages. Additionally, Af7462 and As1788 primer sets were designed based on LAMP-based SNPs typing for the specific detection of African and Asian lineages. The RT-LAMP assays detected specifically African and Asian lineages, with the limit of detection range from 0.17 FFU/ml - 2.3x102 FFU/ml. As ZIKV viremia ranges between 102 to 106 PFU/ml or 103-106 copies/mL, the data indicate that the viremia range of clinical samples is within our detection range. Because of the high specificity and sensitivity and ease of use, the re...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Bui TT, Moi ML, Morita K, Hasebe F Tags: Jpn J Infect Dis Source Type: research

Genetic characterization of a schoolgirl infection with novel reassortant H5N6 avian influenza virus.
Abstract A 10-year-old schoolgirl was laboratory confirmed infection with H5N6 subtype highly pathogenic avian influenza (HPAI) virus in Suzhou city in November, 2018 (JSSZ01). Hemagglutinin (HA) gene of this H5N6 virus belonged to 2.3.4.4 H5 clade and the HA linkage peptide of JSSZ01 strain was RERRRKR↓GLF with multiple basic amino acids. Q226L and G228S mutations were not observed, but S128P, S137A, T160A substitutions in receptor binding sites (RBS) were discovered. Resistance mutation D198N in neuraminidase (NA) protein was also identified in this strain. Besides, 11 amino acids (59-69AAs) deletion was id...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Dong Z, Ya X, Wang D, Liu C, Shen Q, Xia Y Tags: Jpn J Infect Dis Source Type: research

Skin and soft tissue infections caused by different genotypes of PVL-positive community-acquired methicillin-resistant Staphylococcus aureus strains.
Abstract Panton-Valentine leukocidin (PVL) is a causative agent of lethal necrotizing pneumonia and is associated with epidemic strains of community-acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA). PVL-producing strains have rarely been isolated in Japan. However, the isolation frequency of PVL-positive CA-MRSA has been rapidly increasing in recent years. To investigate the relevance of the pvl genes (lukS/F-PV) and clinical traits in epidemic S. aureus strains, we genotyped four PVL-positive CA-MRSA strains isolated from patients with skin and soft tissue infections and measured their susceptibil...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Hanawa T, Shimoda-Komatsu Y, Araki K, Ohyama M, Onishi H, Kamiya S, Matsuda T Tags: Jpn J Infect Dis Source Type: research

A novel reporter gene assay for pyrogen detection.
This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably transfected with the NF-κB reporter gene as a pyrogenic marker. The RGA could detect different types of pyrogens, including the lipopolysaccharide of gram-negative bacteria, the lipoteichoic acid of gram-positive bacteria, and the zymosan of fungi, and a good dose-effect relationship was observed in terms of NF-κB activity. The limits of detection of the RGA to those pyrogens were 0.03 EU/ml, 0.001 μg/ml, and 1μg/ml, respectively. The method had good precision and accuracy and could b...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: He Q, Yu C, Wang L, Ni Y, Zhang H, Du Y, Gao H, Wang J Tags: Jpn J Infect Dis Source Type: research

Prevalence and Genotypes Distribution of HR-HPV among Chinese Women in Sichuan Province.
This study aimed to determine HR-HPV prevalence and genotypes distribution among Chinese women living in Sichuan province. A total of 3089 women were recruited from the Third people's,Hospital of Chengdu in Sichuan province. HR-HPV genotyping were assessed by real-time quantitative PCR. Up to 720 cases (23.3%) were HR-HPV positive. 81.1% (584/720) were detected for a single HR-HPV genotype and 18.9% (136/720) for multiple genotypes. The most frequently detected HPV genotype was HPV52, followed by HPV58, HPV16, and HPV51. Age subgroup analysis showed two peaks for the frequencies of HPV infections, one for the group of wo...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Li L, Chen Y, Chen J, Su Q, Tang J, Yang P, Cui Q, Tang Y Tags: Jpn J Infect Dis Source Type: research

Declining Vibrio parahaemolyticus and Salmonella, increasing Campylobacter and persisting no-rovirus food poisonings: inference derived from Food Poisoning Statistics of Japan.
Abstract In Japan, from 2000 to 2018, for Vibrio parahaemolyticus and Salmonella food poisonings, the annual number of the outbreaks and that of the patients decreased exponentially though the size of the individual outbreaks (the number of patients per outbreak) tended to become larger. For food poisonings caused by Campylobacter, the annual number of the outbreaks increased exponentially while the outbreak size became smaller and the annual number of the patients remained almost unchanged. For food poisoning caused by norovirus, both the number of the outbreaks and that of the patients remained high throughout. ...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Comparison of clinical features of hepatitis A in people living with HIV between pandemic in 1999-2000 and that in 2017-2018 in a metropolitan area of Japan.
Abstract Since 2017, hepatitis A virus (HAV) infection has been an epidemic among men who have sex with men (MSM) in Japan. We experienced 11 MSM patients with hepatitis A who were also infected with HIV. In 1999-2000, we also experienced 5 HIV-infected patients with hepatitis A. Since conditions of current HIV-infected patients have changed owing to recent progress in anti-HIV therapies, we compared clinical features of hepatitis A between patients in 2017-2018 and those in 1999-2000. By comparing patients' background characteristics, we found the CD4/CD8 ratio was significantly higher in the 2017-2018 group. Aft...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Koga M, Lim LA, Ogishi M, Satoh H, Kikuchi T, Adachi E, Sugiyama R, Kiyohara T, Suzuki R, Muramatsu M, Koibuchi T, Tsutsumi T, Yotsuyanagi H Tags: Jpn J Infect Dis Source Type: research

Effect of metofluthrin-impregnated spatial repellent devices in combination with new long-lasting insecticidal nets (Olyset ® Plus) on pyrethroid-resistant malaria vectors and malaria prevalence - Field trial in south-eastern Malawi.
Effect of metofluthrin-impregnated spatial repellent devices in combination with new long-lasting insecticidal nets (Olyset® Plus) on pyrethroid-resistant malaria vectors and malaria prevalence - Field trial in south-eastern Malawi. Jpn J Infect Dis. 2019 Oct 31;: Authors: Kawada H, Nakazawa S, Shimabukuro K, Ohashi K, Kambewa EA, Foster Pemba D Abstract Long-lasting insecticidal nets (LLINs) experience some operational problems that reduce their effectiveness, such as limited spaces for hanging, biting of mosquitoes outdoors, a shift of key biting time from midnight to dawn or dusk, and developme...
Source: Japanese Journal of Infectious Diseases - October 31, 2019 Category: Infectious Diseases Authors: Kawada H, Nakazawa S, Shimabukuro K, Ohashi K, Kambewa EA, Foster Pemba D Tags: Jpn J Infect Dis Source Type: research

Epidemiological survey of Babesia divergens Asia lineage in wild sika deer (Cervus nippon) by using direct PCR, in Japan.
Abstract Babesia divergens is the major causal agent of zoonotic human babesiosis in Europe. In Japan, a parasite genetically closely related to the European B. divergens, B. divergens Asia lineage, was detected in wild sika deer (Cervus nippon) in our previous study. We therefore conducted large epidemiological survey by combining lineage specific PCR system and blood direct PCR. The infection rate of the Asia lineage was 6.6% (116/1747) throughout the country, where Hokkaido (45%), Nagano (17%), Iwate (12%), Gunma (11%) and Yamanashi (11%) were highly enzootic (>10%) among 30 prefectures examined. European B....
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Zamoto-Niikura A, Hagiwara K, Imaoka K, Morikawa S, Ishihara C, Hanaki KI Tags: Jpn J Infect Dis Source Type: research

Detection of Borrelia DNA in tick species collected from vegetation and wild animals in Fukuoka, Japan.
Abstract We surveyed the presence of Borrelia spp. in ticks collected from vegetation by flagging and from wild animals from May 2017 to November 2018 in Fukuoka, located in the northern Kyushu area of Japan. A total of 1,601 ticks were collected and morphologically separated into 9 species: Ixodes turdus, I. ovatus, Amblyomma testudinarium, Haemaphysalis flava, H. formosensis, H. kitaokai, H. longicornis, H. hystricis, and H. megaspinosa. The ticks were segregated into 561 pools and nested PCR was used to detect borrelial DNA. Borrelia turdi and Borrelia sp. HM were identified in two of the 561 pools. This is the...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Nakayama S, Kobayashi T, Nakamura A, Yoshitomi H, Song Y, Ashizuka Y Tags: Jpn J Infect Dis Source Type: research

Abdominal Aortic Graft Infection Caused by stG485.0, ST29 Streptococcus dysgalactiae subsp. equisimilis.
Abstract In recent years, the prevalence of invasive Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections has increased gradually throughout the world, including Japan. Here, we report the case of an abdominal aortic graft infection caused by stG485.0, ST29 SDSE in an elderly patient with diabetes. An 86-year-old man who had undergone an abdominal aortic aneurysm (not infected) repair with a bifurcated graft 10 years ago was referred to our hospital after being suspected of having abdominal aortic graft infection through computed tomography. He underwent surgery, and the pus developed between the aneury...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Kakuta R, Shimizu T, Goto H, Endo S, Kanamori H, Ohta N, Nakano R, Yano H, Katori Y, Kaku M Tags: Jpn J Infect Dis Source Type: research

Molecular identification of the etiologic agent of human gnathostomosis in an endemic area of Mexico.
This study aims to develop a duplex-PCR method of the ITS-2 region for differentiating G. binucleatum and G. turgidum parasites that coexist in the same endemic area and to identify the Gnathostoma larvae recovered from biopsies of two gnathostomosis patients from Sinaloa, Mexico. The duplex PCR established based on the ITS-2 sequence showed that the length of the amplicons was 321 bp for G. binucleatum and 226 bp for G. turgidum. The amplicons from the AL3A of both patients were 321 bp. Furthermore, the length and composition of these amplicons were identical to that deposited in GenBank as G. binucleatum (accession No. J...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Díaz-Camacho SP, Parra-Unda JR, Ríos-Sicairos J, Delgado-Vargas F Tags: Jpn J Infect Dis Source Type: research

Molecular characteristics of novel mono-reassortant G9P[8] rotavirus A strains possessing the NSP4 gene of the E2 genotype detected in Tokyo, Japan.
Abstract Rotavirus A (RVA) has been detected from patients with gastroenteritis even after vaccine introduction in Japan. To investigate the circulating RVA strains, the RVA-positive stool specimens obtained in Tokyo in 2017 and 2018 were analyzed by next-generation sequencing. A total of 50 and 21 RVA samples were obtained in 2017 and 2018, respectively. In 2017, G2P[4] (40.0%) was the most prevalent strain, followed by G3P[8] (DS-1-like) (28.0%), G8P[8] (10.0%), G3P[8] (Wa-like) (8.0%), G9P[8]-E1 (8.0%), and mixed infection (6.0%). In 2018, G3P[8] (DS-1-like) (28.6%) and G9P[8]-E2 (28.6%) were the prevalent stra...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Fujii Y, Oda M, Somura Y, Shinkai T Tags: Jpn J Infect Dis Source Type: research

Comparison of Haemophilus influenzae Seroprevalence in Serum Samples Collected from 0-5-year-old Children in Japan in 1980, 1995, 2010, and 2012.
Abstract Haemophilus influenzae type b (Hib) causes various invasive infections, such as meningitis, septic arthritis, and pneumonia, especially in children under 5 years of age. Despite Hib vaccines protect Hib infection, seroepidemiological surveys of Hib infections have not been conducted systematically in Japan thus far. We analyzed 1,338 serum samples from 0-5-year-old children that were provided by the National Serum Reference Bank of the National Institute of Infectious Diseases (Tokyo, Japan). Serum levels of the anti-polyribosylribitol phosphate (PRP) IgG antibody against the Hib were determined using an ...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Arai S, Satoh H, Okuno H, Morino S, Oishi K, Tanaka-Taya K Tags: Jpn J Infect Dis Source Type: research

Antiviral and Virucidal Activities of Umesu Phenolics on Influenza Viruses.
Abstract Umesu phenolics were purified from the salt-extracts of Japanese apricot (Nanko-mume cultivar of Prunus mume Sieb. et Zucc.). Characterization of umesu phenolics revealed that they inhibited the multiplication of influenza virus and several other RNA and DNA viruses, when added to the culture media of the infected cells. In addition to these antiviral activities, the phenolics significantly decreased a plating efficiency of influenza virus, if present in the virus inoculum. More drastic effects were observed in virucidal activity; the infectivity of several strains of influenza viruses decreased less than...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Ikeda K, Nishide M, Tsujimoto K, Nagashima S, Kuwahara T, Mitani T, Koyama AH Tags: Jpn J Infect Dis Source Type: research

Comparison of Reverse Transcription Loop-Mediated Isothermal Amplification Method with SYBR Green Real-Time RT-PCR and Direct Fluorescent Antibody Test for diagnosis of rabies.
This study tried to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of rabies virus RNA in the brain samples in comparison with SYBR Green Real Time RT-PCR test as a molecular technique and Direct Fluorescent Antibody Test as a serologic method. In this study, RT-LAMP was developed to rabies diagnosis. Six primers were designed, based on the nucleoprotein (N) of rabies virus. The sensitivity and specificity of SYBR Green Real-Time RT-PCR and RT-LAMP methods were also determined.RT-LAMP optimized at 58˚C for 60 min and the sensitivity and specificity of RT-LAMP an...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Naji E, Fadajan Z, Afshar D, Fazeli M Tags: Jpn J Infect Dis Source Type: research

Prevalence of Virulence Genes of Diarrheagenic Escherichia coli in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh.
Abstract Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and diarrheic patients in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). Invasion-related gene virB had the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB for diffuse adhesion and second with aggR for aggregative adhesion. These three genes were specific to patients. In contrast, Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. eae for attaching and effacing lesion, and elt and est of enterotoxins were detected from all thre...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Parvej MS, Alam MA, Shono M, Zahan MN, Masuma Parvez MM, Karim Ansari W, Jowel MS, Uddin MS, Kage-Nakadai E, Rahman MT, Nishikawa Y Tags: Jpn J Infect Dis Source Type: research

Diversity in Genotype Distribution of Mycoplasma pneumoniae Obtained from Children and Adults.
Abstract The aim of this study was to explore whether there was any special genotype responsible for the high prevalence of Mycoplasma pneumoniae (M.pneumoniae) infection in children. A total of 247 M. pneumoniae - DNA positive clinical specimens including 200 from children and 47 from adults collected in Beijing, China, during the same period were analyzed. We performed P1-restriction fragment length polymorphism analysis (RFLP), multi-locus variable number tandem repeat analysis (MLVA) and detected the macrolide resistance-associated mutations in 23S rRNA of the clinical specimens. In the present study, we obser...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Yan C, Yang H, Sun H, Zhao H, Feng Y, Xue G, Li S, Cui J, Ni S Tags: Jpn J Infect Dis Source Type: research

Fluctuations in antibody titers against enterovirus D68 in pediatric sera collected in a community before, during, and after a possible outbreak.
Abstract Previously, we reported a hospital-based epidemiological study on enterovirus (EV)-D68 infection among children during the autumn of 2015, which indirectly inferred an outbreak in Sendai, Japan. In the present study, we collected sera from children (aged 0-6 years; without symptoms of infectious diseases) in the Sendai community during four sample collection periods (1 year before, 6 months before, immediately after, and 1 year after the possible outbreak period). Immunity levels against EV-D68 infection in the children were analyzed by assaying the neutralization antibody titers of the sera. Further, it ...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Kadji FMN, Nishimura H, Okamoto M, Sato K, Ohmiya S, Ito H, Suzuki A, Nagai Y, Oshitani H Tags: Jpn J Infect Dis Source Type: research

Two cases of dengue virus type 2 (DENV-2) infection in a Japanese couple returning from Maldives in the dengue outbreak in 2018.
Abstract Annually, more than 1.2 million travelers from other countries visit Maldives for sightseeing, business and honeymoons. In 2018, the largest dengue fever outbreak occurred and more than 3,200 people were affected. During this outbreak, we encountered a newly married Japanese couple returning from Maldives on their honeymoon in October 2018, both of which got infected by dengue virus type 2 (DENV-2) during the travel. The number of imported dengue fever cases from Maldives may increase, hence, physicians should keep a sharp watch on up-to-date dengue outbreak information all over the world. PMID: 3147...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Mutoh Y, Moriya A, Yasui Y, Saito N, Takasaki T, Hiramatsu S, Izuchi T, Umemura T, Ichihara T Tags: Jpn J Infect Dis Source Type: research

Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation.
kgün M Abstract In addition to tube drains, pleural empyema is treated with antibiotics and anti-inflammatory drugs. We aimed to evaluate the anti-inflammatory activity of roflumilast combined with linezolid in a rat model of pleural empyema induced by Staphylococcus aureus.A total of 40 rats were separated into 7 groups: Sham (n=4), S. aureus inoculation (n=6), S. aureus + 10 mg/kg linezolid (n=6), S. aureus + 5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg linezolid+5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg roflumilast (n=6), and S. aureus + 10 mg/kg linezolid + 10 mg/kg roflumilast (n=6). Linezolid w...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Kerget B, Araz Ö, Kerget F, Erol HS, Özmen S, Halıcı Z, Akgün M Tags: Jpn J Infect Dis Source Type: research

Dengue virus serotype-4 during the 2015-2017 epidemic: emergence of a novel genotype IIa of DENV-4 in the Philippines.
Abstract Dengue remains a major public health problem in the Philippines. Here, we determined the circulating serotypes in the Philippines during the 2015-2017 outbreaks by using a total of 678 serum samples that were collected from 537 individual dengue patients. Following an increase in the percentage of DENV-4 patients in recent years, we conducted a comprehensive molecular epidemiology analysis on the DENV-4 strains isolated recently in the Philippines. While two genotypes of DENV-4 have been isolated in the Philippines since 1956, the GI and GIIa which were the DENV-4 strains isolated in this study were close...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Luz MAV, Nabeshima T, Moi ML, Dimamay MTA, Pangilinan LS, Dimamay MPS, Matias RR, Mapua CA, Buerano CC, de Guzman F, Tria ES, Natividad FF, Daroy MLG, Takemura T, Hasebe F, Morita K Tags: Jpn J Infect Dis Source Type: research

A quicker Tzanck smear with methylene blue stain in diagnosis of herpesvirus skin infections: a comparative study with Giemsa stain.
PMID: 31474704 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Wantavornprasert K, Chottawornsak N, Hurst CP, Asawanonda P, Hansasuta P, Rerknimitr P Tags: Jpn J Infect Dis Source Type: research

A Case Report of Cat-to-Human Transmission of Severe Fever with Thrombocytopenia Syndrome Virus.
PMID: 31366857 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kida K, Matsuoka Y, Shimoda T, Matsuoka H, Yamada H, Saito T, Imataki O, Kadowaki N, Noguchi K, Maeda K, Mochizuki Y, Kishimoto T Tags: Jpn J Infect Dis Source Type: research

Conjunctivitis due to human adenovirus type 2 variant identified during epidemic keratoconjunctivitis surveillance in Japan.
PMID: 31366858 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kaneko H, Hanaoka N, Konagaya M, Tsukahara-Kawamura T, Kobayashi M, Nakagawa H, Hatano H, Ikuta K, Fujimoto T Tags: Jpn J Infect Dis Source Type: research

Risk assessment of dengue autochthonous infections in Tokyo in summer, especially in the period of the 2020 Olympic Games.
This study is aiming at analyzing a risk of autochthonous dengue infections in Tokyo in summer and also an additional risk in the Olympiad by using a mathematical model. We developed a stochastic transmission model with the cooperation of seasonal factors which influence much on the transmission cycle of dengue virus, and carried out stochastic simulations for each of the scenarios provided adequately. We found that (1) the incidence of dengue autochthonous infections is predicted to be small number cases; (2) the local climate makes a great influence on a scale of dengue autochthonous infections; (3) the incidence reaches...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Ishikawa H, Shimogawara R Tags: Jpn J Infect Dis Source Type: research

Comparison of in vivo activity of garenoxacin and levofloxacin in a murine pneumonia mixed-infection model by Streptococcus pneumoniae and Parvimonas micra.
Abstract Community-acquired pneumonia (CAP) can involve mixed-infection. However, there are few studies investigating its antimicrobial activity in mixed-infection. The aim of this study was to compare the in vivo antimicrobial activity of GRNX and levofloxacin (LVFX) against Streptococcus pneumoniae and Parvimonas micra in a murine pneumonia mixed-infection model. S. pneumoniae D-6888 and P. micra No. 242 were used in this study. Antimicrobial activity was calculated for each isolate as the change in bacterial count of lungs (Δlog10 CFU/mL) obtained in the treated mice after 24 h compared with the count in ...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kato H, Hagihara M, Yokoyama Y, Suematsu H, Asai N, Koizumi Y, Yamagishi Y, Mikamo H Tags: Jpn J Infect Dis Source Type: research

High Prevalence of Hepatitis E Virus Infection in Imported Cynomolgus Monkeys in Japan.
Abstract Cynomolgus monkey is an important experimental animal for hepatitis E virus (HEV) infection. In Japan, cynomolgus monkeys are imported mainly from Asian countries for use at animal facilities and institutions. However, the status of HEV infection in cynomolgus monkeys remains unclear. A total of 187 pairs of serum and fecal samples were collected from cynomolgus monkeys (Macaca fascicularis) imported from China and Cambodia to detect anti-HEV IgG and IgM antibodies as well as HEV RNA. Based on an enzyme-linked immunosorbent assay using HEV-like particles derived from genotype 3 HEV as the antigen, 184 of ...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Zhang W, Yoshizaki S, Ami Y, Suzaki Y, Takeda N, Muramatsu M, Li TC Tags: Jpn J Infect Dis Source Type: research

Higher genetic diversity and viral evolution on key regions of envelope gene are associated with broader neutralizing antibody responses: report of two chronic HIV infected cases.
Abstract Dozens of broadly neutralizing antibodies (bnAbs) have been identified from chronically infected HIV-1 patients, but it is still unclear what determines the acquisition of broad neutralizing activities. Two chronic HIV-1 infected cases with similar autologous neutralizing activities were followed up for two years to study viral evolution of the envelope gene and the neutralizing activity against autologous and heterologous viruses. The neutralization activities against homologous viruses gradually increased in both patients. HA172 eventually developed a cross-clade neutralizing antibodies response, with a...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Shen S, Tian W, Ji Y, Gao Y, Zhang M, Han X, Shang H Tags: Jpn J Infect Dis Source Type: research

Analysis of clinical isolates of extended-spectrum β-lactamase-producing bacteria with developed primer and probe sets to detect blaCTX-M, blaTEM, and blaSHV using a fully automated gene detection system.
In this study, we evaluated extended-spectrum β-lactamase (ESBL)-producing bacteria with the newly developed primer and probe sets to detect blaCTX-M, blaTEM, and blaSHV using BD MAXTM, a fully automated multiplex polymerase chain reaction assay system. In 36 isolates confirmed to have any one of blaCTX-M, blaTEM, or blaSHV by whole-genome sequencing, the product accurately detected each gene without influenced by the presence of other β-lactamase genes. In nine control strains which do not harbor either blaCTX-M, blaTEM, or blaSHV did not cross react with the product. In 191 strains phenotypically determined ESB...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Abe Y, Nakamura K, Kaji D, Takahashi H, Aoki K, Kuse H, Okada H, Ohta K, Ohashi K, Takano Y, Ishii Y, Kanemitsu K Tags: Jpn J Infect Dis Source Type: research

Viral and atypical bacterial respiratory infections in a university teaching hospital.
In this study, we aimed to detect mutiple viral and bacterial agents in the respiratory samples of inpatients by real-time PCR (RT-PCR). Nasopharyngeal swab and broncho alveolar lavage samples' results from the inpatients with respiratory infection symptoms at the Uludag University Hospital between 01.12.2015 and 31.03.2018 were investigated. DNA/RNA was extracted with EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) in EZ1 (Qiagen, Belgium). R-GENE® Real-Time PCR (Biomerioux, France) kit was used to detect Influenza A, Influenza B, RSV, human Meta-pneumo virus (hMPV), Rhino/Enterovirus, Adenovirus (AdV), human Bocavirus (hBo...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Agca H, Ener B Tags: Jpn J Infect Dis Source Type: research

Laboratory evaluation of rapid antigen detection tests for more-sensitive detection of Respiratory syncytial virus antigen.
Abstract We evaluated two currently available rapid antigen detection tests (RADTs) for Respiratory syncytial virus (RSV), Sofia® RSV FIA and BinaxNOW RSV Card (BinaxNOW). Between November 2017 and February 2018, 395 nasopharyngeal swabs were collected from children diagnosed with acute respiratory infections, and were evaluated with these RADTs, reverse transcription-quantitative real-time PCR (RT-qPCR), and a direct immunofluorescence assay (DFA). The sensitivity of Sofia® RSV FIA (80.82%) was significantly higher than that of BinaxNOW (53.42%) when RT-qPCR was used as the standard. This was confirmed wi...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Sun Y, Deng J, Qian Y, Zhu R, Wang F, Tian R, De R, Zhao L Tags: Jpn J Infect Dis Source Type: research

Dengue Virus in Traveler Returning to Japan from the Democratic Republic of the Congo, 2015.
Abstract Dengue fever (DF) is a mosquito-borne disease and significant global public health problem. Although limited serologic surveys in the literature suggest endemic DF in many parts of Africa, DF cases in Africa are generally underreported due to the lack of diagnostic testing and systematic surveillance, and thus, little is known about the phylogenetic profile of circulating strains. In April 2015, DF was diagnosed in a Japanese national returned from the Democratic Republic of the Congo (DRC). Dengue virus 1 (DENV-1) RNA was detected from the patient's serum sample by real-time reverse transcription PCR. Ph...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Yamamoto SP, Kasamatsu Y, Kanbayashi D, Kaida A, Shirano M, Kubo H, Goto T, Iritani N Tags: Jpn J Infect Dis Source Type: research