Effect of Host Factors on Neutrophil Functions in Response to Burkholderia pseudomallei in Healthy Thai Subjects.
This study therefore indicated the importance of age, glycemic control, and antibody levels in the activity of neutrophils in melioidosis. PMID: 25410558 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Saengmuang P, Kewcharoenwong C, Tippayawat P, Nithichanon A, Buddhisa S, Lertmemongkolchai G Tags: Jpn J Infect Dis Source Type: research

Comparison of Two Methods of Bacterial DNA Extraction from Human Fecal Samples Contaminated with Clostridium perfringens, Staphylococcus aureus, Salmonella Typhimurium, and Campylobacter jejuni.
In this study, 2 methods of DNA extraction were evaluated for use in conjunction with the screening system Rapid Foodborne Bacterial Screening 24 (RFBS24), which employs multiplex real-time SYBR Green polymerase chain reaction (SG-PCR) and can simultaneously detect 24 target genes of foodborne pathogens in fecal DNA samples. The QIAamp DNA Stool mini kit (Qkit) and Ultra Clean Fecal DNA Isolation Kit (Ukit) were used for bacterial DNA extraction from fecal samples artificially inoculated with Clostridium perfringens, Staphylococcus aureus, Salmonella Typhimurium, and Campylobacter jejuni. SG-PCR and simplex real-time quant...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Kawase J, Kurosaki M, Kawakami Y, Kashimoto T, Tsunomori Y, Sato K, Ikeda T, Yamaguchi K, Watahiki M, Shima T, Kameyama M, Etoh Y, Horikawa K, Fukushima H, Goto R, Shirabe K Tags: Jpn J Infect Dis Source Type: research

Impact of population size on incidence of rubella and measles in comparison with that of other infectious diseases.
Abstract A strong dependency of rubella and measles epidemics on population size was confirmed by 2 types of plots: the cumulative frequency distribution of number of cases per prefecture and the slope of the log-log plots of number of cases per prefecture on the y-axis vs. prefecture population size on the x-axis. These parameters were found to be constant and unique to each infectious agent. The broader cumulative frequency distribution and steeper slope of the log-log plots were characteristic to measles and rubella, i.e., a higher population size was correlated with a disproportionate high incidence of measles...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Epidemiology of scrub typhus and the eschars patterns in South Korea from 2008 to 2012.
Abstract In South Korea, scrub typhus is the most common acute febrile illness in autumn. We analyzed scrub typhus cases reported from 2008 to 2012 to describe the epidemiology of scrub typhus as well as eschar patterns. A total of 30,478 cases were reported from 2008 to 2012; the incidence rates were higher in the southern and western regions of South Korea. The common clinical symptoms of confirmed scrub typhus cases from 2010 to 2012 were fever/chills (95.2%), eschars (78.9%), and myalgia (61.7%). The primary sites of eschars were the lower extremities (19.0%), abdomen/waist (13.4%), and axilla (11.5%) in men a...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Park JH, Kim SJ, Youn SK, Park K, Gwack J Tags: Jpn J Infect Dis Source Type: research

A Retrospective, Hospital-Based Study to Determine the Incidence of Rotavirus Hospitalizations among Children Less than 5 Years of Age over a 10-Year Period (2001-2011) in Akita Prefecture, Japan.
Abstract Rotavirus is the most common cause of severe gastroenteritis in children worldwide. This retrospective, cross-sectional study was undertaken in a sentinel hospital that provides the only pediatric beds for the local population with an average of 4,400 children aged
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Kinoshita S, Noguchi A, Miura S, Nakagomi T, Nakagomi O, Takahashi T Tags: Jpn J Infect Dis Source Type: research

Associations between Co-Detected Respiratory Viruses in Children with Acute Respiratory Infections.
Abstract Viruses are the major etiological agents of acute respiratory infections (ARIs) in young children. Although respiratory virus co-detections are common, analysis of combinations of co-detected viruses has never been conducted in Japan. Nineteen respiratory viruses or subtypes were surveyed using multiplex real-time PCR on 1,044 pediatric (patient age
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Kaida A, Kubo H, Takakura K, Sekiguchi J, Yamamoto SP, Kohdera U, Togawa M, Amo K, Shiomi M, Ohyama M, Goto K, Hase A, Kageyama T, Iritani N Tags: Jpn J Infect Dis Source Type: research

Mycobacterium tuberculosis Pili Promote Adhesion to and Invasion of THP-1 Macrophages.
Abstract Central to the paradigm of the pathogenesis of Mycobacterium tuberculosis is its ability to attach to, enter, and subsequently survive in host macrophages. However, little is known regarding the bacterial adhesins and invasins involved in this interaction with host macrophages. Pili are cell-surface structures produced by certain bacteria and have been implicated in adhesion to and invasion of phagocytes in several species. M. tuberculosis pili (MTP) are encoded by the Rv3312A (mtp) gene. In the present study, we assessed the ability of a Δmtp mutant and an mtp-complemented clinical strain to adhere...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Ramsugit S, Pillay M Tags: Jpn J Infect Dis Source Type: research

Epidemiology and Laboratory Diagnoses of Rubella in Hokkaido District during the Nationwide Outbreak in Japan, 2011-2013.
We report the epidemiology and laboratory diagnostic results of rubella cases from 2011 to 2013 in Hokkaido district, Japan. A total of 150 cases were officially reported as rubella; 102 (68%) involved males and 48 (32%) involved females. The highest proportion of cases were notified in 40-49-year-old age group among males and the 20-29-years-old age group among females. Forty-six cases (25 males and 21 females) had not been vaccinated, and 17 had been vaccinated, whereas 87 had the unknown vaccination status. Eighty-three cases (55.3%) showed the 3 typical principal rubella symptoms (fever, rash, and lymphadenopathy). Sev...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Miyoshi M, Komagome R, Ishida S, Nagano H, Okano M Tags: Jpn J Infect Dis Source Type: research

Evaluation of the In Vivo Efficacy of Novel Monosubstituted Sulfonylureas against H37Rv and Extensively Drug-Resistant Tuberculosis.
Abstract Sulfonylureas have been regarded as potential drug candidates against tuberculosis (TB) because they can inhibit the biosynthesis of branched-chain amino acids by targeting acetohydroxyacid synthase (AHAS). We demonstrated previously that novel monosubstituted sulfonylureas showed potent in vitro activities against TB. In the current study, we further explored the anti-TB activity of monosubstituted sulfonylureas in a mouse model. Compounds 30 and 31 exhibited the most efficacy: a single intragastric administration at a dose of 250 mg/kg led to a reduced lung bacterial count, and the dose of 500 mg/kg ach...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Liu Y, Bao P, Wang D, Li Z, Li Y, Tang L, Zhou Y, Zhao W Tags: Jpn J Infect Dis Source Type: research

A Severe Case of Lemierre Syndrome with Streptococcus constellatus Infection.
Abstract A 76-year-old Japanese male presenting with high fever, headache, and disturbance of consciousness was hospitalized. Contrast computed tomography revealed thrombophlebitis in the internal jugular vein and abscesses in the posterior neck region, pharynx, and pterygoid muscle. Streptococcus constellatus infection was confirmed by culture of blood samples, and the patient was diagnosed with Lemierre syndrome. In addition to the administration of antibiotics and anticoagulants, abscess drainage was performed. S. constellatus should be considered as a causative bacterium in elderly patients with Lemierre syndr...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Shimada M, Morinaga Y, Kitazaki T, Fukuda M, Hashiguchi K, Yanagihara K, Kohno S Tags: Jpn J Infect Dis Source Type: research

Genetic Diversity of Campylobacter jejuni Isolates from Korea and Travel-Associated Cases from East and Southeast Asian Countries.
Abstract Forty domestic and travel-associated Campylobacter jejuni isolates were analyzed by profiling 7 pathogenic genes (cdtB, cadF, Cj0131, ciaB, racR, wlaN, and virB11) along with multilocus sequence typing (MLST) and antimicrobial susceptibility testing. cdtB, cadF, and Cj0131 were present in all isolates, whereas virB11 was not detected in either domestic or travel-associated isolates. ciaB was present in all domestic isolates and 94% of travel-associated isolates. The respective detection rates of racR and wlaN in domestic and travel-associated isolates were 94% and 71% and 35.3% and 23%, respectively. MLST...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Cha I, Kim NO, Nam JG, Choi ES, Chung GT, Kang YH, Hong S Tags: Jpn J Infect Dis Source Type: research

Construction of New Primer Sets for Corresponding to Genetic Evolution of Human Adenoviruses in Major Capsid Genes through Frequent Recombination.
This study contributes to the accumulation of correct information regarding genetic diversity and evolution in the worldwide HAdV surveillance. PMID: 25410569 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Matsushima Y, Nakajima E, Ishikawa M, Kano A, Komane A, Fujimoto T, Hanaoka N, Okabe N, Shimizu H Tags: Jpn J Infect Dis Source Type: research

Nonspecificity of Primers for Escherichia albertii Detection.
PMID: 25410570 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Maeda E, Murakami K, Okamoto F, Etoh Y, Sera N, Ito K, Fujimoto S Tags: Jpn J Infect Dis Source Type: research

Epidemiological Investigation and Seroprevalence of Human Parainfluenza Virus in Mie Prefecture in Japan during 2009-2013.
PMID: 25410571 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Yano T, Fukuta M, Maeda C, Akachi S, Matsuno Y, Yamadera M, Kobayashi A, Nagai Y, Kusuhara H, Kobayashi T, Amano H, Nishinaka T, Ochiai H, Watanabe M, Nakamura H, Suga S, Ihara T Tags: Jpn J Infect Dis Source Type: research

Serodiagnosis of Mycobacterium avium Complex Disease in Humans: Translational Research from Basic Mycobacteriology to Clinical Medicine.
Abstract Rapid and accurate diagnosis of infectious diseases, including mycobacterial disease such as tuberculosis (TB) and diseases due to nontuberculous mycobacteria (NTM), is a very important element of global health. The gold standard in diagnosis of mycobacterial diseases remains clinical examination, combined with direct microscopic examination of sputum and culture of bacteria. Culture of slowly growing mycobacteria, including Mycobacterium tuberculosis and NTM (such as M. avium complex: MAC), can take up to 4 to 6 weeks, and in 10-20% of cases the bacillus is not successfully cultivated. Diagnosis of MAC p...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Kobayashi K Tags: Jpn J Infect Dis Source Type: research

Comparison of the Detection Rates of RT-PCR and Virus Culture Using a Combination of Specimens from Multiple Sites for Enterovirus-Associated Encephalomyelitis during Enterovirus 71 Epidemic.
Abstract Enterovirus (EV) 71 may cause severe neurological illness in the pediatric population. The present study aimed to compare the detection rates of reverse transcription-polymerase chain reaction (RT-PCR) with pan-EV/EV71 type- specific primers and virus culture (VC) for the identification of EV and EV71 using specimens from multiple sites. In total, specimens from throat/rectal swabs, cerebrospinal fluid (CSF), and blood from 66 patients diagnosed with EV encephalomyelitis were subjected to both RT-PCR and VC for detecting the presence of pan-EV and EV71. The results revealed that the positive RT-PCR rate w...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Tsai JD, Tsai HJ, Lin TH, Chang YY, Yang SH, Kuo HT Tags: Jpn J Infect Dis Source Type: research

The Effect of the Different Treatment Protocols on Virological and Immunological Responses in Patients with HIV/AIDS.
This study analyzed the differences between the virological and immunological responses in patients with HIV/AIDS subjected to different treatment protocols. The cases treated with HAART were divided into groups on the basis of treatment protocols. The groups were evaluated with respect to the differences by comparing the virological and immunological responses prior to treatment and at 12 and 24 weeks of therapy. Six different treatment protocols were applied in the treated patients. As the largest clusters, the lopinavir/ritonavir-based patient group (Group 1, n = 29) and efavirenz-based patient group (Group 2, n = 18) w...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Sari ND, Fincanci M, Soysal F Tags: Jpn J Infect Dis Source Type: research

Clinical manifestations of children with microbiologically confirmed pertussis infection and antimicrobial susceptibility of isolated strains in a regional hospital in Japan, 2008-2012.
Abstract We conducted a retrospective study in 57 children (median age, 3.5 years; range, 1 month-14.5 years) with microbiologically confirmed pertussis infection over a recent 4-year period in a regional hospital in Japan. We obtained nasal swabs from all patients for Bordetella pertussis isolation as well as performed B. pertussis DNA detection using loop-mediated isothermal amplification (LAMP). Of the 57 cases, 34 (60%) were culture-positive and 57 (100%) were LAMP-positive. The frequency of each symptom was as follows: typical paroxysmal cough for over 14 days, 96% (55/57); paroxysms, 86% (49/57); posttussive...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Horiba K, Nishimura N, Gotoh K, Kawaguchi M, Takeuchi S, Hattori F, Isaji M, Okai Y, Hosono H, Takemoto K, Ozaki T Tags: Jpn J Infect Dis Source Type: research

Etiology of acute conjunctivitis due to coxsackievirus a24 variant, human adenovirus, herpes simplex virus, and Chlamydia in beijing, china.
In conclusion, HAdV followed by CA24v, chlamydia, and HSV were the most common causative agents of acute conjunctivitis in Beijing. Comprehensive, continuous surveillance and advanced laboratory techniques are needed for further studies. PMID: 25241684 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Li J, Yang Y, Lin C, Li W, Yang Y, Zhang Y, Jia L, Li X, Chen L, Wang Q Tags: Jpn J Infect Dis Source Type: research

Characteristics of group B streptococcus isolated from infants with invasive infections: a population-based study in Japan.
Abstract Group B Streptococcus (GBS) is one of the leading causes of neonatal bacterial infections. Population-based surveillance of GBS-related invasive diseases among newborns and infants from 10 prefectures in Japan was performed between 2007 and 2012. The characteristics of cases and isolated GBS are described in this study. The incidence rate of GBS-related invasive diseases was 0.13 per 1,000 live births. Analysis of GBS samples obtained from 60 invasive cases showed that the most frequent serotypes were III (48.3%), Ia (30.0%), and Ib (10.0%). All isolates were susceptible to penicillin G, ampicillin, cefot...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Chang B, Wada A, Hosoya M, Oishi T, Ishiwada N, Oda M, Sato T, Terauchi Y, Okada K, Nishi J, Akeda H, Kamiya H, Ohnishi M, Ihara T, Japanese Invasive Disease Study Group Tags: Jpn J Infect Dis Source Type: research

Phylogenetic Analysis of the Oriental-Palearctic-Afrotropical Members of Anopheles (Culicidae: Diptera) Based on Nuclear rDNA and Mitochondrial DNA Characteristics.
Abstract The phylogenetic relationships of Anopheles spp. at the junction of Oriental, Palearctic, and Afrotropical regions in the Iranian plateau were investigated using molecular markers. A 711-bp mitochondrial DNA cytochrome oxidase C subunit I (COI) fragment and the entire second internal transcribed spacer (ITS2) region (286-576 bp) of the nuclear ribosomal DNA (rDNA-ITS2) were sequenced from 14 and 28 taxa, respectively. The analyses included 12 species within Anopheles and 4 within the Myzorhynchus Series of the subgenus Anopheles, 8 within Neocellia, 6 within Myzomyia, 3 within Paramyzomyia, and 1 within t...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Karimian F, Oshaghi MA, Sedaghat MM, Waterhouse RM, Vatandoost H, Hanafi-Bojd AA, Ravasan NM, Chavshin AR Tags: Jpn J Infect Dis Source Type: research

Characteristics of Geographical Spread and Temporal Accumulation of the 2009 Influenza A (H1N1) Epidemic in Japan Based on National Surveillance Data.
This study aimed to describe the geographical spread and temporal accumulation of cases during the 2009 influenza A (H1N1) epidemic in Japan. Using a method adopted by the National Epidemiological Surveillance of Infectious Diseases (NESID) to identify epidemics, we described the frequency and temporal change of the 2009 influenza A (H1N1) epidemic among designated public health center areas in Japan. We calculated the proportion of public health center areas with an epidemic by week from 1999 to 2010. The 2009 influenza A (H1N1) epidemic had several distinguishing features compared with previous seasonal influenza epidemi...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Ohta A, Hashimoto S, Murakami Y, Kawado M, Taniguchi K, Tada Y, Shigematsu M, Nagai M Tags: Jpn J Infect Dis Source Type: research

Sensitive and rapid detection of campylobacter species from stools of children with diarrhea in Japan by the loop-mediated isothermal amplification method.
Abstract We detected Campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in Kyoto using a commercial loop-mediated isothermal amplification (LAMP) kit with a fluorescent detection reagent after DNA extraction. The sensitivity and specificity were 100% in comparison with those of semi-nested PCR for the differentiation of Campylobacter jejuni and Campylobacter coli. Fourteen of the 20 samples were already determined as C. jejuni by the culture method. All 20 samples were also positive for C. jejuni by the PCR method. Among the 58 cultured samples, the sensitivity of the cu...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Ushijima H, Nishimura S, Thongprachum A, Shimizu-Onda Y, Tran DN, Pham NT, Takanashi S, Dey SK, Okitsu S, Yamazaki W, Mizuguchi M, Hayakawa S Tags: Jpn J Infect Dis Source Type: research

Organization of nosocomial infection control measures and local networks for infectious disease control in middle-scale hospitals in Japan.
Abstract The aims of this study were to assess nosocomial infection control measures at middle-scale hospitals throughout Japan. Of the 823 hospitals participating in this questionnaire-based survey, more than half of the middle-scale hospitals have implemented nosocomial infection control measures, including infection surveillance or infection control rounds, while acknowledging a shortage of infection control staff. These hospitals most frequently consulted public health centers to obtain information and advice. Improved nosocomial infection control in middle-scale hospitals requires sufficient staffing and a lo...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Mori-Yoshikawa N, Ohmagari N, Kirikae T Tags: Jpn J Infect Dis Source Type: research

Identification of a Novel Metallo-β-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in Pseudomonas aeruginosa Isolates from Egypt.
In conclusion, the presence of a new variant of blaVIM, blaVIM-28, on a new organization of class 1 integron having ISPa21 increases the possibility of dissemination of resistance genes within the integron in P. aeruginosa among hospitalized patients in Egypt. PMID: 25241690 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: El-Mahdy TS Tags: Jpn J Infect Dis Source Type: research

Oseltamivir-Resistant Seasonal A(H1N1) and A(H1N1)pdm09 Influenza Viruses from the 2007/2008 to 2012/2013 Season in Nara Prefecture, Japan.
We examined the incidence of oseltamivir-resistant seasonal A(H1N1) and A(H1N1)pdm09 influenza viruses from the 2007/2008 to 2012/2013 season in Nara Prefecture, Japan. To detect the oseltamivir resistance marker in neuraminidase (NA), 365 influenza viruses (60 seasonal A(H1N1) and 305 A(H1N1)pdm09) were sequenced. The H275Y mutation in the NA gene, which confers resistance to oseltamivir, was identified in 93.8% (30/32) of seasonal A(H1N1) viruses that were circulating during the 2008/2009 season. Moreover, the detection rate of oseltamivir-resistant A(H1N1)pdm09 viruses was 4.1% (3/74) and 2.8% (5/180) in the 2009/2010 a...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Yoneda M, Okayama A, Kitahori Y Tags: Jpn J Infect Dis Source Type: research

Detection and Genotyping of Rubella Virus from Exanthematous Patients Suspected of Having Measles Using Reverse Transcription-PCR.
Abstract Between July 2012 and March 2013, a total of 133 clinical specimens from 47 patients suspected of having measles were collected for virological surveillance in Aichi Prefecture, Japan. Facing the rubella epidemic, the reverse transcription (RT)-PCR protocol for measles virus (MeV) was modified to simultaneously detect rubella virus (RUBV) in these clinical specimens. As a result, 30 specimens from 15 patients were positive for RUBV and 8 specimens from 3 patients were positive for MeV. The RUBV genotype analysis for the samples from 13 patients revealed 12 samples as 2B and 1 sample as 1E. The results pro...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Yasui Y, Mori Y, Adachi H, Kobayashi S, Yamashita T, Minagawa H Tags: Jpn J Infect Dis Source Type: research

Alteration in Serum Levels of Inflammatory Cytokines during Parainfluenza Virus Type 1 Infection in Patients with Severe Multiple Disabilities.
Abstract An epidemic of parainfluenza virus type 1 (PIV1) infection occurred in a hospital ward housing patients with severe motor and intellectual disabilities. Twenty-three infected patients exhibited persistent high fever for 4-16 days and decreased lymphocyte counts. One-half of the symptomatic patients had increased blood monocyte counts and the other half progressed to bronchitis or pneumonia. We also compared levels of 27 cytokines in the sera of 21 patients during the acute and normal phases of infection. Cytokine levels were measured with a bead immunoassay performed using the Luminex Multiplex System. Se...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Matsuda S, Kiyota N, Yoshizumi M, Noda M Tags: Jpn J Infect Dis Source Type: research

Seroprevalence of crimean-congo hemorrhagic Fever in southeastern bulgaria.
Abstract Crimean-Congo hemorrhagic fever (CCHF), which is endemic in Bulgaria, is caused by the Crimean-Congo hemorrhagic fever virus (CCHFV). The seroprevalence of CCHFV in southeastern Bulgaria was examined in this study. For this purpose, a total of 751 human blood samples were collected and examined by indirect immunofluorescence assay. In addition, a questionnaire was completed for every participant. Anti-CCHFV antibodies were detected in 3.20% (24/751) of the tested sera. None of the seropositive individuals had a history of CCHF. The results indicate that the proportion of positive findings increase with ag...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Gergova I, Kamarinchev B Tags: Jpn J Infect Dis Source Type: research

Human rhinovirus infection in children with cystic fibrosis.
This study highlights that RV is frequently found in the respiratory secretions of patients with CF and the impact of HRV-A appears higher than that of the other HRV types in patients with pulmonary exacerbations. PMID: 25241695 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Esposito S, Daccò V, Daleno C, Gambazza S, Montinaro V, Bisogno A, Principi N, Colombo C Tags: Jpn J Infect Dis Source Type: research

In Vitro Activity of Novel Oxazolidinone Analogs and 13 Conventional Antimicrobial Agents against Clinical Isolates of Staphylococcus aureus in Beijing, China.
In this study, we investigated the prevalence of MRSA in the PLA 309 Hospital located in Beijing, China, and we determined the susceptibility of S. aureus to novel oxazolidinone analogs and 13 conventional antimicrobial agents. It was shown that drug resistant patterns were severe among the clinical isolates, and the average rate of MRSA among the S. aureus strains was 56.3%. All the S. aureus strains tested were completely susceptible to linezolid, but the minimum inhibitory concentration distribution drifted upwards. Among the novel oxazolidinone analogs, sy142 exhibited more potent antimicrobial activity than linezolid ...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Lei H, Jiang Y, Wang D, Gong P, Li Y, Dong Y, Dong M Tags: Jpn J Infect Dis Source Type: research

Toxoplasma gondii Infection: Seroprevalence and Associated Risk Factors among Primary School Children in the Capital Area of the Republic of the Marshall Islands.
Abstract Although Toxoplasma gondii infection is commonly prevalent in the tropical/subtropical regions, the prevalence of toxoplasmosis in the Republic of the Marshall Islands (RMI) remains unclear. A seroepidemiological survey was undertaken for investigating T. gondii infection in primary school children (PSC) using the latex agglutination (LA) test in the capital area of RMI. Information on demographic characteristics and environmental risk factors was collected via a structured questionnaire. Logistic regression model was applied for multivariate analysis. The overall infection rate of T. gondii was found to ...
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Fu CJ, Chuang TW, Lin HS, Wu CH, Liu YC, Langinlur MK, Lu MY, Hsiao WW, Fan CK Tags: Jpn J Infect Dis Source Type: research

A gastroenteritis outbreak attributed to sapovirus genogroup v in Yokohama, Japan.
PMID: 25241698 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Usuku S, Kumazaki M Tags: Jpn J Infect Dis Source Type: research

Influenza H7N9 Epidemic in China: Monitoring Case Fatality Rate and Epidemiology Reconstitution Using Two-Population Model.
PMID: 25241699 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

A novel complex recombinant form of type 48-related human adenovirus species d isolated in Japan.
Abstract Volume 67, no. 4, p. 282-287, 2014. Page 282, footnote *Corresponding author: Mailing address: "Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan" should read "Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan."Volume 67, no. 4, p. 282-287, 2014. Page 284, Figure 1 should appear as shown below. PMID: 25241700 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 25, 2014 Category: Infectious Diseases Authors: Fujimoto T, Yamane S, Ogawa T, Hanaoka N, Ogura A, Hotta C, Niwa T, Chiba Y, Gonzalez G, Aoki K, Koyanagi KO, Watanabe H Tags: Jpn J Infect Dis Source Type: research

Application of surface-linked liposomal antigens to the development of vaccines that induce both humoral and cellular immunity.
Abstract The first characteristic identified in surface-linked liposomal antigens was the ability to induce antigen-specific, IgE-selective unresponsiveness. These results remained consistent even when different coupling procedures were employed for antigens with liposomes or for liposomes with different lipid components. The potential usefulness of surface-linked liposomal antigens for application to vaccine development was further investigated. During this investigation, a significant difference was observed in the recognition of liposomal antigens by antigen-presenting cells between liposomes with different lip...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Uchida T, Taneichi M Tags: Jpn J Infect Dis Source Type: research

Spanish Flu, Asian Flu, Hong Kong Flu, and Seasonal Influenza in Japan under Social and Demographic Influence: Review and Analysis Using the Two-Population Model.
Abstract When cumulative numbers of patients (X) and deaths (Y) associated with an influenza epidemic are plotted using the log-log scale, the plots fall on an ascending straight line generally expressed as logY = k(logX - logN0). For the 2009 H1N1 influenza pandemic, the slope k was ~0.6 for Mexico and ~2 for other countries. The two-population model was proposed to explain this phenomenon (Yoshikura H. Jpn J Infect Dis. 2012;65:279-88; Yoshikura H. Jpn J Infect Dis. 2009;62:411-2; and Yoshikura H. Jpn J Infect Dis. 2009;62:482-4). The current article reviews and analyzes previous influenza epidemics in Japan...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Genetic Classification of Listeria monocytogenes Serotype 4b Strains, Including Epidemic Clones, Isolated from Retail Meat in the Tokyo Metropolitan Area.
Abstract A food-borne pathogen, Listeria monocytogenes serotype 4b, has been frequently isolated from patients with listeriosis, and numerous outbreaks of listeriosis are associated with this serotype. In the present study, we performed subtyping of L. monocytogenes serotype 4b strains on the basis of genetic analyses. Thirty-four isolates of serotype 4b were classified into 8 genotypes, namely genotypes 12, 15, 16, 17, 18, 23, 24, and 25, on the basis of the sequence for the partial iap gene. Genetic analyses revealed that genotype 16 and genotypes 24 and 25 belong to epidemic clone I (ECI) and ECII, respectively...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Ochiai Y, Mochizuki M, Yamada F, Takano T, Hondo R, Ueda F Tags: Jpn J Infect Dis Source Type: research

Analysis of bacterial pathogens causing acute diarrhea on the basis of sentinel surveillance in shanghai, china, 2006-2011.
Abstract Acute diarrhea is the most common infectious disease worldwide and its causes vary from one region to another. We aimed to analyze the spectrum and epidemiological characteristics of pathogens from 22,386 outpatients with acute diarrhea on the basis of surveillance data from Shanghai, China, during 2006-2011. The following 8 pathogens were isolated and identified using standard methods: Salmonella, Shigella, Vibrio cholerae, V. parahaemolyticus, enteropathogenic Escherichia coli, enterotoxigenic E. coli, enteroinvasive E. coli, and enterohemorrhagic E. coli. In total, 2,234 strains of pathogens were obtai...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Zhang Y, Zhao Y, Ding K, Wang X, Chen X, Liu Y, Chen Y Tags: Jpn J Infect Dis Source Type: research

High Incidence of Community-Acquired Pneumonia among Rapidly Aging Population in Japan: A Prospective Hospital-Based Surveillance.
Abstract The age-group-specific incidence and etiological patterns of community-acquired pneumonia (CAP) have not been fully established in Japan. A 2-year prospective surveillance was conducted in Kochi city, Western Japan. All CAP patients aged ≥15 years who visited a community-based hospital were enrolled in the study. Clinical samples were examined by conventional bacterial culture and urinary antigen tests, and 6 bacterial pathogens and 16 respiratory viruses were identified from sputum samples by multiplex polymerase chain reaction assays. The age-group-specific incidence of CAP was estimated using a popu...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Takaki M, Nakama T, Ishida M, Morimoto H, Nagasaki Y, Shiramizu R, Hamashige N, Chikamori M, Yoshida L, Ariyoshi K, Suzuki M, Morimoto K Tags: Jpn J Infect Dis Source Type: research

Bath water contamination with legionella and nontuberculous mycobacteria in 24-hour home baths, hot springs, and public bathhouses of nagano prefecture, Japan.
Abstract Bath water samples were collected from 116 hot springs, 197 public bathhouses, and 38 24-hour home baths in Nagano Prefecture, Japan, during the period of April 2009 to November 2011, for determining the presence and extent of contamination with Legionella and nontuberculous mycobacteria. Cultures positive for Legionella were observed in 123 of the 3,314 bath water samples examined. The distribution and abundance of Legionella and/or combined contamination with Legionella and nontuberculous mycobacteria were investigated to clarify the contamination levels. The abundance of Legionella was demonstrated to ...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Kobayashi M, Oana K, Kawakami Y Tags: Jpn J Infect Dis Source Type: research

A novel complex recombinant form of type 48-related human adenovirus species d isolated in Japan.
The objective of this study was to document the evolutionary features of a novel isolate (HAdV_Chiba_E086/2012) obtained from the eye swab of a patient with conjunctivitis in Japan. Viral DNA was extracted from the isolate to sequence the whole genome by the Sanger method and aligned with available genome sequences of HAdV-Ds. The phylogenetic trees of the nucleotide sequences of the penton base, hexon, and fiber genes and the E3 region showed that HAdV_Chiba_E086/2012 is closest to HAdV genotype 65 (HAdV-GT65), HAdV-48, HAdV-GT60 and HAdV-22 at 98%, 99%, 95% and 98% identity, respectively, suggesting that this isolate is ...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Fujimoto T, Yamane S, Ogawa T, Hanaoka N, Ogura A, Hotta C, Niwa T, Chiba Y, Gonzalez G, Aoki K, Koyanagi KO, Watanabe H Tags: Jpn J Infect Dis Source Type: research

Antibiotic Susceptibility of Neisseria gonorrhoeae Isolates from Guangzhou, China, during 2002-2011.
Abstract Antibiotic susceptibility of Neisseria gonorrhoeae in Guangzhou during 2002-2011 showed that resistance to penicillin and ciprofloxacin was high, while ceftriaxone remained effective although there was a trend towards reduced sensitivity. PMID: 25056075 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Zheng H, Yang B, Wu X, Huang J, Zeng W, Xue Y, Qin X, Lan Y, Tang S, Ou J, Li Y Tags: Jpn J Infect Dis Source Type: research

Varicella infection complicated by marked thrombocytopenia.
We report a rare case of adult varicella complicated by marked thrombocytopenia. A 49-year-old woman presented with fever and rash for 3 days. Blood examination revealed marked thrombocytopenia (2.7 × 10(4)/μL). Varicella infection was diagnosed after elevated levels of varicella zoster virus IgM and IgG antibodies were observed 2 weeks later. In this case, thrombocytopenia was due to varicella infection, and the mechanism was estimated to be non-immunological. Because varicella infection complicated by thrombocytopenia may result in fatal bleeding, thrombocytopenia in patients with varicella warrants close attent...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Shibusawa M, Motomura S, Hidai H, Tsutsumi H, Fujita A Tags: Jpn J Infect Dis Source Type: research

Evaluation of clinical and laboratory findings of pediatric and adult patients with oropharyngeal tularemia in Turkey: a combination of surgical drainage and antibiotic therapy increases treatment success.
Abstract We analyzed the clinical and laboratory findings of both pediatric and adult patients with oropharyngeal tularemia. We also compared the therapeutic outcomes of patients who underwent surgical drainage of lymph nodes early or late during antibiotic therapy. A total of 68 patients with oropharyngeal tularemia, including 26 children and 42 adults, were enrolled in this study. The average duration between symptom onset and hospital admission was 20.8 days (4-60 days) in the pediatric group and 32.6 days (4-90 days) in the adult group (P = 0.009). The most frequently observed clinical symptoms were sore throa...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Gozel MG, Engin A, Altuntas EE, Salk I, Kaya A, Celik C, Dokmetas I, Bakir M, Elaldi N Tags: Jpn J Infect Dis Source Type: research

The first measles outbreak caused by imported genotype d9 measles virus in shandong province, china, 2013.
This study represents a measles outbreak caused by the genotype D9 measles virus (MeV), which was imported by Burmese individuals. Urine and throat swab specimens were collected from suspected measles cases. Viruses were isolated, and a 634-bp target fragment of the N gene was amplified by reverse transcription-PCR and sequenced. Phylogenetic results indicated that the 6 isolates belonged to genotype D9 MeV. Through appropriate prevention and control measures, the transmission of genotype D9 MeV was interrupted. Genotype D9 MeV was isolated for the first time in Shandong and was imported by Burmese individuals. PMID: ...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Fang X, Sun J, Zhang Y, Wang C, Song Y, Song L, Xu Q, Li B, Chen P, Li H, Xu A Tags: Jpn J Infect Dis Source Type: research

The Occurrence of Severe Rotavirus Gastroenteritis in Children under 3 Years of Age before and after the Introduction of Rotavirus Vaccine: a Prospective Observational Study in Three Pediatric Clinics in Shibata City, Niigata Prefecture, Japan.
Abstract The occurrence of severe rotavirus gastroenteritis (RVGE) in children under 3 years of age before and after the introduction of rotavirus vaccine was prospectively surveyed in three pediatric clinics in Shibata City, Niigata Prefecture, Japan, during the 2011 and 2012 RVGE epidemic seasons. In this observational study, a significantly lower occurrence of severe RVGE among severe gastroenteritis cases was observed in 2012. The incidence rate of severe RVGE among outpatients in 2012 was significantly lower than that in 2011. Despite the significant reduction in severe RVGE, the results must be interpreted w...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Oishi T, Taguchi T, Nakano T, Sudo S, Kuwajima H, Shibata RVGE Study Group Tags: Jpn J Infect Dis Source Type: research

Stability of JC Virus DNA in Cerebrospinal Fluid Specimens Preserved with Guanidine Lysis Buffer for Quantitative PCR Testing.
Abstract Quantitative PCR testing for JC virus (JCV) DNA in the cerebrospinal fluid (CSF) is one of the diagnostic standards for progressive multifocal leukoencephalopathy (PML). The present study was conducted to examine its reliability using CSF specimens that had been preserved with guanidine lysis buffers in commercial nucleic acid extraction kits under different conditions. When CSFs were mixed with guanidine buffers, JCV DNA levels were not statistically reduced even after storage for 1 month at room temperature or for 3 months at -80℃, compared with the control samples. In addition, the JCV DNA level was ...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Nakamichi K, Lim CK, Saijo M Tags: Jpn J Infect Dis Source Type: research

Recent Progress toward Measles Elimination in Hokkaido, Japan, during 2011-2012.
Abstract Laboratory diagnoses for measles were performed in a total of 97 cases in Hokkaido, Japan, during 2011-2012. Two patients were confirmed to be positive for measles virus (MV), both of whom lived in the Iburi district of Hokkaido. Molecular analysis of the nucleotide sequences of the nucleoprotein (N) gene revealed that these 2 strains had high homology with each other and belonged to the genotype D8. The onset interval of these cases and epidemiological data suggested that MV transmission had occurred between them and then terminated. Phylogenetic analysis of the N gene revealed that the strains identifie...
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Miyoshi M, Komagome R, Ishida S, Kikuchi M, Sato H, Ito H, Nagano H, Okano M Tags: Jpn J Infect Dis Source Type: research

Antimicrobial Resistance among Campylobacter Isolates Obtained from Retail Chicken Meat and Offal Products in Japan.
In this study, we aimed to demonstrate the overall trend in antimicrobial resistance among Campylobacter isolates obtained from chicken meat and offal products collected from a wide geographic area throughout Japan. Resistance to Enrofloxacin was most frequently observed, with significantly higher rate of resistance among isolates obtained from offal (55.6%) than from meat (27.3%) samples (p = 0.05). These results highlight need for a better understanding of the characteristics of Campylobacter isolates obtained from chicken meat and offal products. PMID: 25056082 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 29, 2014 Category: Infectious Diseases Authors: Hidano A, Yamamoto T, Hayama Y, Muroga N, Kobayashi S, Nishida T, Tsutsui T Tags: Jpn J Infect Dis Source Type: research