MePIC, Metagenomic Pathogen Identification for Clinical Specimens.
Abstract Next-generation DNA sequencing technologies have led to a new method of identifying the causative agents of infectious diseases. The analysis comprises three steps. First, DNA/RNA is extracted and extensively sequenced from a specimen that includes the pathogen, human tissue and commensal microorganisms. Second, the sequenced reads are matched with a database of known sequences, and the organisms from which the individual reads were derived are inferred. Last, the percentages of the organisms' genomic sequences in the specimen (i.e., the metagenome) are estimated, and the pathogen is identified. The first...
Source: Japanese Journal of Infectious Diseases - January 25, 2014 Category: Infectious Diseases Authors: Takeuchi F, Sekizuka T, Yamashita A, Ogasawara Y, Mizuta K, Kuroda M Tags: Jpn J Infect Dis Source Type: research

Evaluation of a Double-Disk Synergy Test with a Common Metallo-β-Lactamase Inhibitor, Mercaptoacetate, for Detecting NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii.
Evaluation of a Double-Disk Synergy Test with a Common Metallo-β-Lactamase Inhibitor, Mercaptoacetate, for Detecting NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii. Jpn J Infect Dis. 2014;67(1):66-8 Authors: Wachino J, Matsui M, Tran HH, Suzuki M, Suzuki S, Shibayama K PMID: 24451107 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - January 25, 2014 Category: Infectious Diseases Authors: Wachino J, Matsui M, Tran HH, Suzuki M, Suzuki S, Shibayama K Tags: Jpn J Infect Dis Source Type: research

Foodborne Outbreak Associated with Escherichia coli O112ac:H19 in China.
PMID: 24451108 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - January 25, 2014 Category: Infectious Diseases Authors: Jin Y, Yang H, Xiong Y, Zhao A, Gan L, Liu Q, Lin L, Chen Y, Li J, Li B, Wu J, Lin W Tags: Jpn J Infect Dis Source Type: research

Multicolor flow cytometric analyses of CD4+ T cell responses to Mycobacterium tuberculosis-related latent antigens.
Abstract Although IFN-γ release assays (IGRAs) provide increased specificity over tuberculin skin tests, the early and sensitive detection of reactivation of latently infected Mycobacterium tuberculosis is required to control tuberculosis (TB). Recently, a multicolor flow cytometry has been developed to study CD4(+) T cell cytokine responses (IFN-γ/IL-2/TNF-α) to purified protein derivatives (PPD) and M. tuberculosis-specific antigens (ESAT-6/CFP-10) and provided useful information regarding anti-TB immunity. However, the diagnostic relevancy remains uncertain. Here, we analyzed three additional ...
Source: Japanese Journal of Infectious Diseases - December 18, 2013 Category: Infectious Diseases Authors: Yamashita Y, Hoshino Y, Oka M, Matsumoto S, Ariga H, Nagai H, Makino M, Ariyoshi K, Tsunetsugu-Yokota Y Tags: Jpn J Infect Dis Source Type: research

Determination of In Vitro Activities of Polymyxin B and Rifampin in Combination with Ampicillin/Sulbactam or Cefoperazone/Sulbactam against Multidrug-Resistant Acinetobacter baumannii by the E-test and Checkerboard Methods.
Abstract The aim of this study was to investigate the in vitro activities of polymyxin B (PB) and rifampin (RIF) in combination with ampicillin/sulbactam (AS) or cefoperazone/sulbactam (CS) against 20 multidrug-resistant Acinetobacter baumannii (MDR-AB) isolates by the checkerboard and E-test methods. Fractional inhibitory concentration index (FICI) values were defined as synergy, FICI ≤ 0.5; additivity, 0.5
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Cetin ES, Tekeli A, Ozseven AG, Us E, Aridogan BC Tags: Jpn J Infect Dis Source Type: research

Low Plasma Zinc Levels and Immunological Responses to Zinc Supplementation in HIV-Infected Patients with Immunological Discordance after Antiretroviral Therapy.
Abstract Immunological discordance in HIV-infected patients is associated with a higher risk of mortality and disease progression. Zinc is an essential micronutrient for immune function. A two-phase pilot study including a cross-sectional study to determine plasma zinc levels and a prospective, randomized, placebo-controlled trial to determine immunological responses after zinc supplementation was conducted in HIV-infected patients with immunological discordance in a medical school hospital. Immunological discordance was defined in patients who received antiretroviral therapy, had HIV RNA
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Asdamongkol N, Phanachet P, Sungkanuparph S Tags: Jpn J Infect Dis Source Type: research

Mycobacterium simiae: a Possible Emerging Pathogen in Iran.
In this study, we used molecular methods to detect this organism from patients who were suspected of having tuberculosis. A total of 117 isolates of mycobacteria were evaluated from different regions of Iran. Isolates were identified using phenotypic methods and gene sequencing of 16S rRNA, rpoB, hsp65, and ITS. Of the 117 isolates, 12 M. simiae isolates (10.2%) were identified from different clinical samples, including bronchoalveolar lavage and sputum (n = 8), blood (n = 3), and lymph node biopsy (n = 1). Three isolates (3/12, 25%) were recovered from blood samples of HIV cases when the CD4(+) count was less than 50/μ...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Hashemi-Shahraki A, Darban-Sarokhalil D, Heidarieh P, Feizabadi MM, Deshmir-Salameh S, Khazaee S, Alavi SM Tags: Jpn J Infect Dis Source Type: research

The Role of Superoxide Dismutase in the Survival of Mycobacterium tuberculosis in Macrophages.
Abstract There is a large amount of information available regarding the chemical structure and biological activity of superoxide dismutase (SOD), which is abundantly generated by Mycobacterium tuberculosis in the early stages of growth. SOD is a strong superoxide radical scavenger, which plays a significant role in resisting oxidative stress. On the other hand, SOD mutant strains have been constructed to define the role of this molecule in the immune response to M. tuberculosis infection. These studies have suggested that the absence or attenuation of SOD can motivate innate immunity and SOD avoid destruction or g...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Liao D, Fan Q, Bao L Tags: Jpn J Infect Dis Source Type: research

Helicobacter pylori Infection Is Associated with a Decreased Risk of Tooth Loss in Healthy Japanese Men.
We examined 959 healthy adults who participated in a mass survey. We counted the number of residual teeth and measured both H. pylori stool antigen and serum anti-H. pylori antibodies. Serum pepsinogen levels were measured to determine the presence of atrophic gastritis. Multiple logistic regression analysis was performed after adjustments for age, body mass index (BMI), smoking and alcohol habits, and educational background. In men, H. pylori infection was a significantly reduced risk factor for loss of all the teeth even after adjustments for other factors (OR, 0.32; 95% CI, 0.12-0.81; P
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Shimoyama T, Higuchi H, Matsuzaka M, Chinda D, Nakaji S, Fukuda S Tags: Jpn J Infect Dis Source Type: research

Evaluation of renal involvement in children with crimean-congo hemorrhagic Fever.
Abstract The aim of the present study was to evaluate renal involvement in children with Crimean-Congo hemorrhagic fever (CCHF). Forty-four children infected with CCHF virus and 30 controls were enrolled in the study. Urine neutrophil gelatinase-associated lipocalin (uNGAL) and urine protein levels were measured in the patient and control groups. Clinical and laboratory findings of the patient and control groups were compared. uNGAL levels were higher in the patient group than that in the control group (P
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Deveci K, Uysal EB, Kaya A, Sancakdar E, Alkan F Tags: Jpn J Infect Dis Source Type: research

Influence of Vaccination Dose and Clinico-Demographical Factors on Antibody Titers against Measles, Rubella, Mumps, and Varicella-Zoster Viruses among University Students in Japan.
Abstract To evaluate the influence of vaccination dose and clinico-demographical factors on immune status against measles, rubella, mumps, and varicella viruses among university students, we conducted a case-control study by analyzing serum antibody titers according to past immunization and infection, and perinatal histories, using a multivariate regression model. A total of 1370 medical, paramedical, and pharmaceutical students were included in the analysis. Two or more doses of measles and rubella vaccination yielded notably greater odds ratios for immuno-positivity (9.1; 95% confidence interval (CI), 2.8-28.9 a...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Takeuchi J, Goto M, Kawamura T, Hiraide A Tags: Jpn J Infect Dis Source Type: research

Acute Bacterial Meningitis among Children Admitted into an Iranian Referral Children's Hospital.
Abstract Bacterial meningitis is a serious threat to global health, particularly in developing countries. The aim of this study was to determine the etiological agents of acute bacterial meningitis, its clinical features, and antibacterial susceptibility among Iranian children who were admitted to a referral regional children's hospital. Laboratory data as well as symptoms and signs on admission, organism identification and antibiotic susceptibility results, physical examination findings, and neurologic features of 31 patients with suspected invasive bacterial infection were evaluated, of which 20 (64.5%) had posi...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Mahmoudi S, Zandi H, Pourakbari B, Ashtiani MT, Mamishi S Tags: Jpn J Infect Dis Source Type: research

Emergence of unusual nonfermenting gram-negative nosocomial pathogens in a saudi hospital.
This study aimed to determine the frequency of isolation and prevalence of drug resistance in nonfermenting Gram-negative bacilli (NFGNB) other than Pseudomonas aeruginosa and predisposing factors for the acquisition of nosocomial infections caused by these emerging pathogens in a Saudi tertiary care hospital. A total of 125 nonduplicating NFGNB nosocomial strains were isolated, of these, 68 (54.4%) were Acinetobacter baumannii, 26 (20.8%) Stenotrophomonas maltophilia, 14 (11.2%) Alcaligenes faecalis, 12 (9.6%) Chryseobacterium indologenes, and 5 (4%) Ralstonia pickettii. MICs of 11 antibiotics were determined using the re...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Asaad AM, Al-Ayed MS, Qureshi MA Tags: Jpn J Infect Dis Source Type: research

Comparison of Test Methods for Detecting Metallo-β-Lactamase-Producing Gram-Negative Bacteria.
Comparison of Test Methods for Detecting Metallo-β-Lactamase-Producing Gram-Negative Bacteria. Jpn J Infect Dis. 2013;66(6):512-8 Authors: Hattori T, Kawamura K, Arakawa Y Abstract The recent increase in gram-negative bacteria coproducing multiple classes of β-lactamases has made it difficult to accurately identify metallo-β-lactamase (MBL) producers. In the present study, six methods for detecting MBL producers were compared using 56 gram-negative bacterial isolates that produce various β-lactamases. Sodium mercaptoacetic acid (SMA) and EDTA were used as inhibitors for a double-disk sy...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Hattori T, Kawamura K, Arakawa Y Tags: Jpn J Infect Dis Source Type: research

Severe Sepsis due to Aeromonas aquariorum in a Patient with Liver Cirrhosis.
Abstract Thus far, Aeromonas aquariorum infection has been unrecorded in Korea. Herein, we report a fatal case of A. aquariorum infection in a 77-year-old male patient with liver cirrhosis. The bacterium isolated from a blood culture was initially mistaken as Aeromonas hydrophila using the Vitek2 identification system. In spite of intravenous ceftriaxone therapy, the patient was exacerbated by multiple organ dysfunction. By 4 days after admission, there was no hope for treatment or remission of symptoms and the patient was discharged. In the detailed microbiological investigations, the bacterium was identified as ...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Shin GW, You MJ, Cho HS, Yi SW, Lee CS Tags: Jpn J Infect Dis Source Type: research

Prototheca Algaemia: a Rare but Fatal Opportunistic Infection among Immunocompromised Individuals.
Abstract Infections due to Prototheca spp. are ubiquitous in nature, occurring in both immunocompetent and immunocompromised patients. The study cohort consisted of 14 cases of Prototheca algaemia reported over the past 5 decades and 2 recent cases from study hospitals. Prototheca wickerhamii was the most common species. The overall mortality rate was 62.5%. Prototheca algaemia, a healthcare-associated infection, was observed in immunocompromised patients and was associated with a poor prognosis. PMID: 24270142 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Yeh CT, Li MC, Chuang YC, Hsu HJ, Lee MF, Zhang CC, Ko WC, Tang HJ Tags: Jpn J Infect Dis Source Type: research

Protease-Dependent Hemagglutinin Cleavage Contributes to Alteration in Chicken Hemagglutination by the H3N2 Influenza A Virus.
Abstract The human influenza A virus (H3N2) has been the predominant influenza strain since 1992, and one property of this virus is non-agglutination of chicken erythrocytes [Ch(-) virus]. The Ch(-) virus in our study was able to acquire chicken hemagglutination [Ch(+)] by trypsin passage but not by chymotrypsin passage. Moreover, the trypsin-passaged Ch(+) viruses reacquired the Ch(-) property after a further chymotrypsin passage. In particular, genetic analysis showed no evidence of mutations in the hemagglutinin (HA) gene during either trypsin or chymotrypsin passages: the only differences found were in the HA ...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Yamaoka M, Makino A, Sasahara K, Nastri AM, Krisna LA, Purhito EF, Poetranto ED, Wulandari L, Yudhawati R, Setiawati L, Setyoningrum RA, Shinya K Tags: Jpn J Infect Dis Source Type: research

A Foodborne Outbreak of Gastrointestinal Illness Caused by Enterotoxigenic Escherichia coli Serotype O169:H41 in Osaka, Japan.
We describe our laboratory investigation of a massive foodborne outbreak of gastrointestinal illness caused by enterotoxigenic Escherichia coli (ETEC) serotype O169:H41 that occurred during a 2-day traditional festival held in September 2012 in Osaka Prefecture, Japan. Of 126 customers who patronized a particular Japanese restaurant during the event, 102 developed symptoms of gastrointestinal disease. We isolated strains of ETEC serotype O169:H41 from 1 food sample and from fecal samples collected from 19 of 34 patients and 2 of 4 food handlers. Pulsed-field gel electrophoresis analysis of these isolates suggested that the...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Harada T, Itoh K, Yamaguchi Y, Hirai Y, Kanki M, Kawatsu K, Seto K, Taguchi M, Kumeda Y Tags: Jpn J Infect Dis Source Type: research

In Vitro Antibiotic Susceptibility of Francisella tularensis Isolates from Japan.
Abstract The antibiotic susceptibilities of 36 isolates of Japanese Francisella tularensis, an etiological agent of the zoonotic disease tularemia, were analyzed using the E test. All the isolates were susceptible to ciprofloxacin, doxycycline, erythromycin, and gentamicin but resistant to benzylpenicillin and cephalothin. The susceptibility to seven other β-lactams (aztreonam, cefotaxime, cefoxitin, ceftriaxone, cefuroxime, imipenem, and meropenem) varied among the isolates. These findings suggest that the guidelines for the antibiotic treatment of tularemia issued by the World Health Organization are approp...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Hotta A, Fujita O, Uda A, Sharma N, Tanabayashi K, Yamamoto Y, Yamada A, Morikawa S Tags: Jpn J Infect Dis Source Type: research

Seroprevalence of coxsackievirus b3 in yantai, china.
Abstract Coxsackievirus B3 (CVB3) is an important human pathogen, which is frequently associated with aseptic meningitis and encephalitis in many parts of the world. However, the seroprevalence of this viral infection in China is not well understood; therefore, in the present study, we conducted a serological survey in which 373 serum samples obtained from healthy people belonging to 10 age groups in Yantai city, China were examined. The results revealed that CVB3 is widely distributed in the population with a seroprevalence of 52.3%. The seroprevalence of CVB3 was lowest (3.7%) in the group aged 8-12 months and&g...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Tao Z, Li B, Xu A, Liu Y, Song L, Wang S, Xiong P, Lin X, Song Y Tags: Jpn J Infect Dis Source Type: research

Assessment of the Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Mycoplasma pneumoniae in Pediatric Community-Acquired Pneumonia.
In this study, the efficacy of the loop-mediated isothermal amplification (LAMP) assay for diagnosis of M. pneumoniae pneumonia in clinical practice was prospectively evaluated. From July 2011 to March 2012, 531 children hospitalized for community-acquired pneumonia were enrolled. In all patients, throat swabs were obtained on admission for the detection of M. pneumoniae DNA, and paired serum samples were obtained to assay M. pneumoniae particle agglutination (PA) antibody titers. M. pneumoniae pneumonia was diagnosed by either a positive LAMP assay or an increase of 4-fold or greater in the PA titer. Overall, 271 children...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Gotoh K, Nishimura N, Takeuchi S, Hattori F, Horiba K, Isaji M, Okai Y, Ohshima Y, Hosono H, Takemoto K, Iwata Y, Nakane K, Funahashi K, Ozaki T Tags: Jpn J Infect Dis Source Type: research

Identification of the Source of Francisella tularensis Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis.
Abstract Tularemia is a zoonotic disease caused by Francisella tularensis. Most patients in Japan have reportedly acquired such infections through direct contact with infected Japanese hares. We recently encountered a patient who contracted tularemia after skinning and butchering a dead hare. Because the remains of the hare were available, we attempted to determine whether the patient actually contracted infection by handling the carcass. F. tularensis-specific sequences were successfully amplified by PCR from the patient specimens as well as from the remnants of discarded hare carcass. PCR amplification of the IS...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Fujita O, Hotta A, Uda A, Yamamoto Y, Fujita H, Shinya F, Asano S, Morikawa S, Tanabayashi K, Yamada A Tags: Jpn J Infect Dis Source Type: research

Rapid and Reliable Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus agalactiae.
Abstract Streptococcus agalactiae (group B Streptococcus, GBS) is the leading cause of neonatal sepsis and meningitis and an important pathogen in elderly patients and those with underlying diseases. The diagnosis of GBS infections is primarily based on culture of GBS. Some clinical laboratories perform the Christie-Atkins-Munch-Peterson (CAMP) test for discrimination of GBS from other streptococci. Here, we developed a rapid GBS identification method, i.e., the loop-mediated isothermal amplification (LAMP) method for detecting the cfb gene encoding the CAMP factor. This method detected at least 4 copies of the cf...
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Kimura K, Yanagisawa H, Wachino J, Shibayama K, Arakawa Y Tags: Jpn J Infect Dis Source Type: research

Seroprevalence of a Novel Influenza A (H3N2) Variant Virus in the Japanese Population.
Abstract Antibody distribution to a novel swine-origin influenza A (H3N2) variant virus among different age groups in Japan was determined by hemagglutination-inhibition assay. Japanese children in the age group 1-14 years were at a higher risk than those in older age groups. PMID: 24270150 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Kishida N, Imai M, Xu H, Taya K, Fujisaki S, Takashita E, Tashiro M, Odagiri T Tags: Jpn J Infect Dis Source Type: research

Scrub typhus in South India: a re-emerging infectious disease.
PMID: 24270151 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Stephen S, Kandhakumari G, Pradeep J, Vinithra SM, Siva PK, Hanifah M, Vanithadevi E Tags: Jpn J Infect Dis Source Type: research

Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant Klebsiella pneumoniae Isolate.
Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant Klebsiella pneumoniae Isolate. Jpn J Infect Dis. 2013;66(6):555-7 Authors: Al-Marzooq F, Yusof MY, Tay ST PMID: 24270152 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Al-Marzooq F, Yusof MY, Tay ST Tags: Jpn J Infect Dis Source Type: research

Laboratory Diagnosis and Epidemiology of Avian Influenza A (H7N9) Virus Infection in Humans in Nanchang City, China.
PMID: 24270153 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Zhou X, Li H, Ni X, Liu M, Hu M, Wu J, Xia W, Yang S, Liu Y, Xiong Z, Chen H Tags: Jpn J Infect Dis Source Type: research

Virological analysis of a regional mumps outbreak in the northern island of Japan-mumps virus genotyping and clinical description.
PMID: 24270154 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Okajima K, Iseki K, Koyano S, Kato A, Azuma H Tags: Jpn J Infect Dis Source Type: research

A Case of Atypical Hand-Foot-and-Mouth Disease Caused by Coxsackievirus A6: Differential Diagnosis from Varicella in a Pediatric Intensive Care Unit.
PMID: 24270155 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2013 Category: Infectious Diseases Authors: Yasui Y, Makino T, Hanaoka N, Owa K, Horikoshi A, Tanaka A, Suehiro Y, Shimizu H, Kanou K, Kobayashi M, Konagaya M, Fujimoto T Tags: Jpn J Infect Dis Source Type: research

Outbreak of febrile respiratory illness caused by adenovirus at a South korean military training facility: clinical and radiological characteristics of adenovirus pneumonia.
We described the laboratory and radiological characteristics of patients with adenovirus pneumonia. Among these, 407 cases of FRI and 15 cases of pneumonia were identified through active surveillance (attack rate of FRI, 57.16%; attack rate of pneumonia, 2.11%). Fire training and march training may present environmental risk factors for adenovirus-associated outbreaks. Most symptoms were mild. The most frequent symptom in patients with pneumonia and FRI was cough. Patients with pneumonia were associated with an increased incidence of dizziness (crude odds ratio [cOR], 9.65; 95% confidence interval [CI], 2.38-37.15) and a d...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Hwang SM, Park DE, Yang YI, Park SJ, Lee HK, Kim MJ, Chun BC Tags: Jpn J Infect Dis Source Type: research

Correlation between Complement Component Levels and Disease Severity in Dengue Patients in Indonesia.
In this study, the complement hemolytic activity (CH50) and levels of complement components and related factors in dengue patients in Indonesia were assessed. Based on the number of days since fever onset, DF patients were compared with patients at the DHF pre-critical phase who showed deterioration within 2 days. The mean CH50 values and levels of C2, C4, and factors B and H in the DHF pre-critical phase group were significantly lower than those in the DF group. The mean CH50 values were significantly correlated with C4, factor B, or factor H levels, indicating their responsibility for reduced CH50 values. Furthermore, a ...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Yamanaka A, Hendrianto E, Mulyatno KC, Susilowati H, Ginting AP, Sary DD, Soegijanto S, Konishi E Tags: Jpn J Infect Dis Source Type: research

Changing Burden of HIV/AIDS to Clinical Settings in Northern Thailand over 15 Years.
Abstract We conducted a hospital-based descriptive study to describe the changing pattern of patient numbers, characteristics, and mortality rates among human immunodeficiency virus (HIV)-infected patients in northern Thailand over 15 years. The survival status on October 31, 2010 of all HIV-infected adults who attended an HIV center in a government hospital between 1995 and 2010 was ascertained. In total, 3,706 patients were registered, 2,118 (57.2%) of which were male. The survival status of 3,439 patients (92.9%) was available. In addition, 1,543 deaths were identified out of 12,858 person-year-observations (PY...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Pathipvanich P, Tsuchiya N, Rojanawiwat A, Schmidt WP, Auwanit W, Sawanpanyalert P, Ariyoshi K Tags: Jpn J Infect Dis Source Type: research

Clostridium difficile Infection in Lung Cancer Patients.
This study aimed to analyze predisposing or associated risk factors for CDI in lung cancer patients receiving chemotherapy. This study was a retrospective review of 188 lung cancer patients who were admitted to the Wonkwang University Hospital between 2008 and 2009. Of the 188 patients, 44 were diagnosed with CDI. The albumin levels were significantly lower and the performance status (PS) score was significantly higher in lung cancer patients with CDI than in those without CDI (P
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Hwang KE, Hwang YR, Seol CH, -Park C, Park SH, Yoon KH, Park DS, Lee MK, Jeong ET, Kim HR Tags: Jpn J Infect Dis Source Type: research

Detection and Identification of Genotypes of Prototheca zopfii in Clinical Samples by Quantitative PCR Analysis.
In this study, a specific quantitative PCR system for the detection and identification of Prototheca zopfii genotypes was developed using a TaqMan(®) MGB probe and ResoLight dye. The P. zopfii-specific primers 18PZF1 and 18PZR1 were generated on the basis of the alignment of the small subunit ribosomal DNA domain base sequences of the genera Chlorella and Prototheca obtained from DDBJ/EMBL/GenBank, and the TaqMan(®) MGB probe PZP1 was designed corresponding to this amplification region. Analysis of the melting curves of the amplicons using ResoLight dye was able to differentiate between P. zopfii genotypes 1 and 2....
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Onozaki M, Makimura K, Satoh K, Hasegawa A Tags: Jpn J Infect Dis Source Type: research

Potential role of mannose-binding lectin in intrauterine transmission of hepatitis B virus.
Abstract Intrauterine transmission of hepatitis B virus (HBV) is the main cause of the high prevalence of HBV in endemic areas; however, the mechanisms underlying intrauterine transmission of HBV remain unknown. To explore the role of mannose-binding lectin (MBL), a pattern recognition molecule of the innate immune system, in intrauterine transmission of HBV, we determined MBL levels using an enzyme-linked immunosorbent assay (ELISA) in cord serum of 7 intrauterine-infected neonates and 30 non-infected neonates born to HBV-positive mothers, and 30 control neonates born to HBV-negative mothers. We observed signific...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Wu Y, Zhou Q, Wang H, Tian T, Zhu Q, Wang H, Bai X, Yang X, Wang Z, Dong M Tags: Jpn J Infect Dis Source Type: research

Determination of Enterohemorrhagic Escherichia coli Serotype O165:HNM Infection in a Hemolytic Uremic Syndrome Patient with Adenovirus Seroype 41.
Abstract A 4-year-old girl who was positive for adenovirus according to a rapid immunochromatographic test conducted at a hospital, progressed to hemorrhagic diarrhea and hemolytic uremic syndrome (HUS). The presence of adenovirus serotype 41 (AdV-41) was confirmed by TaqMan real-time PCR and sequence analysis. However, most enteric viral infections cause mild to moderate diarrhea. In the present case, enterohemorrhagic Escherichia coli (EHEC) O165:HNM was isolated concomitantly with AdV-41. In addition, O165 antibody was specifically detected in patient sera. The EHEC isolate was positive for the virulence genes ...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Konno T, Yatsuyanagi J, Takahashi S, Kumagai Y, Wada E, Chiba M, Saito S Tags: Jpn J Infect Dis Source Type: research

Detection and characterization of enteric viruses in flood water from the 2011 thai flood.
This study is the first to detect enteric viral genes in flood water in Thailand. Furthermore, it is the first to detect an NV gene in any type of environmental water in Thailand. These results provide useful information for estimating the risk of flood waterborne viral infection. PMID: 24047738 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Ngaosuwankul N, Thippornchai N, Yamashita A, Vargas RE, Tunyong W, Mahakunkijchareon Y, Ikuta K, Singhasivanon P, Okabayashi T, Leaungwutiwong P Tags: Jpn J Infect Dis Source Type: research

Characterization of DNA polymerase-associated acyclovir-resistant herpes simplex virus type 1: mutations, sensitivity to antiviral compounds, neurovirulence, and in-vivo sensitivity to treatment.
Abstract Acyclovir (ACV)-resistant (ACV(r)) mutants were generated from plaque-purified ACV-sensitive herpes simplex virus type 1 (HSV-1) by culturing the virus in Vero cells in the presence of 2-amino-7-(1,3-dihydroxy-2-propoxymethyl) purine (S2242). Three DNA polymerase (DNApol)-associated ACV(r) HSV-1 generated under ACV selection in a previous study (Suzutani, T., Ishioka, K., De Clercq, E., et al., Antimicrob. Agents Chemother., 47, 1707-1713, 2003) were also included. The sensitivity of the mutants to other antivirals and their neurovirulence were determined. The treatment efficacy of ACV and ganciclovir (GC...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Wang LX, Takayama-Ito M, Kinoshita-Yamaguchi H, Kakiuchi S, Suzutani T, Nakamichi K, Lim CK, Kurane I, Saijo M Tags: Jpn J Infect Dis Source Type: research

Seroprevalence of Measles- and Mumps-Specific Immunoglobulin G among Japanese Healthcare Students Increased during 2007-2012.
Abstract We evaluated the seroprevalence of vaccine-preventable infectious diseases among Japanese healthcare students to create immunization guidelines. Between 2007 and 2012, a total of 1746 Japanese medical, nursing, and paramedical students were serologically screened for measles, mumps, rubella, varicella, and hepatitis B virus (HBV) antibodies at the time of admission. In 2007, the seroprevalence of measles and mumps was 52.7% and 65.6%, respectively. The seroprevalence of measles dramatically increased to 96.6% in 2009 and was then sustained at>90%. The seroprevalence of mumps gradually increased to>8...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Kimura T, Tsunekawa K, Ogiwara T, Tokue Y, Nara M, Inoue T, Obuchi T, Suto C, Ohshima K, Murakami M Tags: Jpn J Infect Dis Source Type: research

Clinical Aspects of Infection with Methicillin-Resistant Staphylococcus aureus USA300 Strain, Generally Regarded as Community-Acquired, in Japan.
In this study, we describe 4 cases of CA-MRSA infections, particularly the USA300 clone. Case 1 involved a 21-year-old man without any remarkable medical history or risk factors of CA-MRSA who suffered from a rapidly progressive infection in his left arm. Case 2 involved a 34-year-old man and Case 3 a 22-year-old man who presented with recurrent and refractory furuncles. Both men were members of a combat sports gym where other members also had skin infections. Case 4 involved a 60-year-old man with lumbar canal stenosis who suffered from surgical site infection 7 days after lumbar laminectomy and posterolateral fusion. Onl...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Nakamura I, Yamaguchi T, Miura Y, Shimizu H, Fukushima S, Mizuno Y, Matsumoto T Tags: Jpn J Infect Dis Source Type: research

Projection of Human Immunodeficiency Virus among High-Risk Groups in Malaysia.
This study aimed to identify trends in HIV-related risk behaviors among recognized high-risk groups and to estimate HIV transmission up to the year 2015. Data and necessary information were obtained from the Ministry of Health Malaysia, published reports from the World Health Organization and United Nations Program on HIV/AIDS, and other articles. The Estimation and Projection Package was used to estimate HIV transmission. The results of the present study revealed that within the high-risk groups, intravenous drug users (IDUs) had the highest prevalence rate of HIV transmission, followed by patients with sexually transmitt...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Mondal MN, Shitan M Tags: Jpn J Infect Dis Source Type: research

Virological Clearance Rate of High-Dose Oseltamivir or Triple-Combination Antiviral Therapy in Complicated 2009 Pandemic Influenza A (H1N1) Infection.
Abstract The 2009 pandemic influenza A (H1N1) was a considerable public health concern worldwide. Significant morbidity and mortality were observed in complicated cases, despite the early administration of neuramidase inhibitors. The limitations of neuramidase inhibitor monotherapy have renewed interest in combination antiviral therapy or higher-dose oseltamivir therapy. Herein, we report our clinical experience and virological outcomes with high-dose oseltamivir or combination antiviral therapy in seriously complicated 2009 pandemic influenza A (H1N1) infection. Eight patients were treated with high-dose oseltami...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Kang SJ, Park KH, Kee SJ, Shin JH, Jung SI, Kwon YS, Jang HC Tags: Jpn J Infect Dis Source Type: research

Plasmid-Mediated Quinolone Resistance Genes, aac(6')-Ib-cr, qnrS, qnrB, and qnrA, in Urinary Isolates of Escherichia coli and Klebsiella pneumoniae at a Teaching Hospital, Thailand.
This study showed a high prevalence of PMQR genes among both organisms. Clinical use of fluoroquinolones for the treatment of infections caused by fluoroquinolone-susceptible strains harboring PMQR genes may lead to decreased therapeutic efficacy. PMID: 24047744 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Pasom W, Chanawong A, Lulitanond A, Wilailuckana C, Kenprom S, Puang-Ngern P Tags: Jpn J Infect Dis Source Type: research

Helicobacter pylori Infection among Children with Phenylketonuria.
This study aimed to determine the frequency of Helicobacter pylori infections in children with phenylketonuria (PKU). Sixty-six children with PKU (35 boys, 31 girls; mean age, 8.2 ± 6.7 years) and 32 outpatient controls (15 boys, 17 girls; mean age, 9.6 ± 4.7 years) were studied. Socioeconomic factors did not differ between the two groups. The frequency of H. pylori infections was higher in patients with PKU (28.1%) than in healthy controls (9.4%). In particular, a higher frequency of infection was detected in patients with PKU with poor metabolic control (51.8%). The frequency of H. pylori infection in patie...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Oztürk Y, Erdur B, Arslansoyu S, Soylar R Tags: Jpn J Infect Dis Source Type: research

Molecular epidemiology of human adenoviruses d associated with epidemic keratoconjunctivitis in osaka, Japan, 2001-2010.
We examined the molecular epidemiology of HAdV-D strains using 39 field isolates collected from epidemic keratoconjunctivitis (EKC) patients from 2001 to 2010 in the province of Osaka, Japan. The molecular types were analyzed by sequencing partial penton base gene, loop 1 in the hexon, and complete fiber genes. Of the 39 isolates, the majority were HAdV-19 (14/39, 35.9%) and -37 (13/39, 33.3%), followed by -53 (4/39, 10.3%) and -54 (8/39, 20.5%). Analysis of annual distribution showed that HAdV-19 and -37 were detected before 2004, whereas HAdV-53 and -54 were first identified in 2001 and 2003, respectively, and persistent...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Hiroi S, Morikawa S, Takahashi K, Komano J, Kase T Tags: Jpn J Infect Dis Source Type: research

The First Report on the Outbreak of OXA-24/40-Like Carbapenemase-Producing Acinetobacter baumannii in Turkey.
In this study, we investigated carbapenem-hydrolyzing class D β-lactamases (CHDLs) in A. baumannii and the molecular epidemiology of CHDL producers at the Dokuz Eylul Hospital, Izmir Turkey, and detected blaOXA-24/40 in a clinical isolate from a patient in the medical intensive care unit (ICU). The specific enzyme type was OXA-72. Additional studies revealed 22 more isolates from 20 patients and that the OXA-72-producing strain caused an outbreak in the medical ICU from September 2012 to March 2013, which still continues. To our knowledge, this is the first report of OXA-24/40 carbapenemases in A. baumannii in Turkey....
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Sarı AN, Biçmen M, Gülay Z Tags: Jpn J Infect Dis Source Type: research

Meningococcemia due to the 2000 Hajj-Associated Outbreak Strain (Serogroup W-135 ST-11) with Immunoreactive Complications.
We present the first reported case of systemic infection with Neisseria meningitidis serogroup W-135 sequence type (ST)-11 in Japan. A 44-year-old woman presented with high fever, sore throat, and fatigue and was diagnosed with N. meningitidis bacteremia. The causative strain was identified as serogroup W-135 ST-11 by polymerase chain reaction and multilocus sequence typing. Approximately 1 month after treatment, she developed high fever, dyspnea, chest pain, and shoulder pain due to pericarditis, polyarthritis, and tenosynovitis, which are all relatively common immunoreactive complications of W-135 ST-11 meningococcal inf...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Yamamoto K, Kato Y, Shindo T, Ujiie M, Takeshita N, Kanagawa S, Kunimatsu J, Tamori Y, Kano T, Okuno R, Takahashi H, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Frequency of Diarrheagenic Escherichia coli among Children in Surabaya, Indonesia.
Abstract Diarrheagenic Escherichia coli (DEC) is a major etiologic agent of childhood diarrhea in developing countries. We investigated the frequency of DEC in stool samples from 125 diarrheal children (age, 1-10 years) and 92 non-diarrheal children in Surabaya, Indonesia. The non-diarrheal children served as healthy controls. DEC was detected in 23 of 125 (18.4%) and 47 of 92 (51.1%) samples in the diarrheal and non-diarrheal children, respectively. Enteropathogenic E. coli was the most prevalent in the non-diarrheal children (25.0%), and its prevalence was significantly higher than that in the diarrheal children (0.8%) (P
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Osawa K, Raharjo D, Wasito EB, Harijono S, Shigemura K, Osawa R, Sudarmo SM, Iijima Y, Shirakawa T Tags: Jpn J Infect Dis Source Type: research

Measles Epidemiology and Survey of Measles Immunity Level among Healthy Population in Baoji City, Shaanxi Province, China.
This study aimed to identify susceptible populations to measles and to establish methods of measles prevention and control. Measles incidence was at the lowest level in 2011 (0.32 per 100,000 populations). Among these patients, the proportion of those younger than 8 months and those older than 27 years of age was relatively high. The overall antibody positive rate was 78.90%, and the average antibody geometric mean concentration was 562.15 mIU/ml. Measles immunity level was high among children aged 8 months to 6 years and low among infants aged 1-7 months. To reduce the incidence of measles among infants younger than 8 mon...
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Zhang X, Kou G, Du H, Ju Z, Zhong L, Cui X, Ling H Tags: Jpn J Infect Dis Source Type: research

Phylogenetic analysis of the nonstructural and structural protein encoding region sequences, indicating successive appearance of genomically diverse sapovirus strains from gastroenteritis patients.
PMID: 24047751 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 21, 2013 Category: Infectious Diseases Authors: Harada S, Tokuoka E, Kiyota N, Katayama K, Oka T Tags: Jpn J Infect Dis Source Type: research