QuantiFERON( ®)-TB Gold In-tube Test for tuberculosis prevention in HIV-infected patients.
QuantiFERON(®)-TB Gold In-tube Test for tuberculosis prevention in HIV-infected patients. Jpn J Infect Dis. 2017 Mar 28;: Authors: Khawcharoenporn T, Phetsuksiri B, Rudeeaneksin J, Srisungngam S, Apisarnthanarak A Abstract Optimal testing strategy for diagnosing latent tuberculosis infection and guiding isoniazid preventive therapy (IPT) is uncertain among HIV-infected patients. A 4-year prospective study was conducted among Thai HIV-infected patients who underwent simultaneous tuberculin skin test (TST) and QuantiFERON®-TB Gold In-tube Test (QFT-IT) at care entry. Based on baseline test resul...
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Khawcharoenporn T, Phetsuksiri B, Rudeeaneksin J, Srisungngam S, Apisarnthanarak A Tags: Jpn J Infect Dis Source Type: research

Prevalence of Diarrheagenic Escherichia coli in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan.
Abstract The source and routes of diarrheagenic Escherichia coli (DEC) remain poorly clarified. To investigate the involvement of domestic animals in the dissemination of DEC, the prevalence of DEC among foods and fecal specimens of cattle, pigs, chickens, healthy carriers, and patients in Osaka and Hyogo, Japan, was investigated using a multiplex real-time PCR assay. The most prevalent virulence genes were astA and eae, with prevalences of 46.8% and 27.4%, respectively. stx1 (26.6%) and stx2 (45.9%) were prevalent in cattle feces, and est (8.5%) and elt (7.6%) were prevalent in pig feces. The afaB gene was the se...
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Wang L, Zhang S, Zheng D, Fujihara S, Wakabayashi A, Okahata K, Suzuki M, Saeki A, Nakamura H, Hara-Kudo Y, Kage-Nakadai E, Nishikawa Y Tags: Jpn J Infect Dis Source Type: research

Antimicrobial susceptibility surveillance of Neisseria gonorrhoeae isolates in Changsha, China during 2003-2015.
Abstract A total of 1294 Neisseria gonorrhoeae isolates obtained in Changsha, China from 2003 to 2015 were examed for susceptibility to penicillin, ciprofloxacin, spectinomycin and ceftriaxone by disc diffusion method and the ceftriaxone MICs of 460 isolates during 2008-2015 were determined by agar dilution method and the ceftriaxone intermediate susceptible isolates were tested for azithromycin susceptibility in addition. The results exhibited that the resistant rate to penicillin and ciprofloxacin were 77.5% and 94.2%, respectively. Only 4 spectinomycin resistant and 2 spectinomycin intermediate susceptible isol...
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Peng T, Lin H, Liu Q, Yang J, Cao W, Ding H, Tang L Tags: Jpn J Infect Dis Source Type: research

Changes in Serum IgA Antibody Levels against the Glycopeptidolipid Core Antigen during Antibiotic Treatment of Mycobacterium avium Complex Lung Disease.
Abstract We evaluated serial changes in the levels of serum immunoglobulin A (IgA) antibody to the glycopeptidolipid (GPL) core antigen during antibiotic treatment in 57 patients with M. avium complex (MAC) lung disease, at baseline (T0) and after 3 months (T3) and 6 months (T6) of treatment. The median patient age was 59 years and 37 (65%) were female. Etiologic organisms included M. avium in 32 (56%) patients and M. intracellulare in 25 (44%). Seven (12%) patients had the fibrocavitary form of the disease on computed tomography. After 12 months of treatment, 42 (74%) patients achieved favorable responses, wherea...
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Jhun BW, Kim SY, Park HY, Jeon K, Shin SJ, Koh WJ Tags: Jpn J Infect Dis Source Type: research

Antimicrobial resistance of Pseudomonas aeruginosa isolated from dogs and cats in primary veterinary hospitals in Japan.
Abstract To investigate antimicrobial resistance, 200 Pseudomonas aeruginosa isolates were collected from dogs and cats in primary veterinary hospitals in Japan. Resistance rates against ciprofloxacin, cefotaxime, gentamicin, amikacin, and fosfomycin were 9, 12.5, 4.5, 2.5, and 35.5%, respectively. One strain (0.5%) displayed resistance to ceftazidime. We did not detect any strain resistant to imipenem nor any multidrug-resistant P. aeruginosa as defined by the Law Concerning the Prevention of Infections and Medical Care for Patients with Infections of the Japanese Ministry of Health, Labour, and Welfare. In addit...
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Yukawa S, Tsuyuki Y, Sato T, Fukuda A, Usui M, Tamura Y Tags: Jpn J Infect Dis Source Type: research

The first isolation of Zika virus from a Japanese patient who returned to Japan from Fiji in 2016.
Abstract Outbreaks of Zika virus (ZIKV) infection in tropical and subtropical regions are cause of worldwide concern and represents a public health emergency. ZIKV was isolated from a 17-year-old patient with fever and maculopapular rash. The patient returned to Japan from the Republic of Fiji in late April 2016. The complete genome sequence of the ZIKV isolate, ZIKV/Hu/S36/Chiba/2016, which might be the first strain to be isolated in Japan, was determined and reported. PMID: 28367888 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 28, 2017 Category: Infectious Diseases Authors: Taira M, Ogawa T, Nishijima H, Yamamoto K, Hotta C, Akita M, Tajima S, Saijo M Tags: Jpn J Infect Dis Source Type: research

Studying DDT susceptibility at discriminating time interval focusing maximum limit of exposure time survived by DDT resistant Phlebotomus argentipes (Diptera: Psychodidae) - an investigatory report.
Abstract Extensive application of routine insecticide i.e., Dichlorodiphenyltrichloroethane (DDT) to control Phlebotomus argentipes (Diptera: Psychodidae), the proven vector of Visceral leishmaniasis (VL) in India, had evoked the problem of resistance/ tolerance against DDT, eventually nullifying the DDT dependent strategies to handle the nuisance caused by them. Because tolerating an hour exposure of DDT is not so challenging for resistant P. argentipes, estimating susceptibility by exposing sand flies to insecticide for just an hour becomes trivial and futile task.Therefore, present bioassay study was carried ou...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Rama A, Kesari S, Das P, Kumar V Tags: Jpn J Infect Dis Source Type: research

Association of IL10, IL4, IFNG and CTLA4 Gene Polymorphisms with Efavirenz Hypersensitivity Reaction in Patients Infected with Human Immunodeficiency Virus.
In conclusion, our data suggest that polymorphisms in regulatory regions of cytokine could modulate an individual's susceptibility to efavirenz hypersensitivity reaction. PMID: 28250252 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Rodrigues RO, Rabenhorst SH, de Carvalho PG, Sasahara GL, Vasconcelos LM, de Arruda ÉA, da Silva SF, Ribeiro IF, Nagao-Dias EA Tags: Jpn J Infect Dis Source Type: research

Geographical Distribution of Japanese Spotted Fever and Tsutsugamushi Disease in Japan - Possible Effect of Environmental Temperature.
PMID: 28250253 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Changes in rotavirus genotypes before and after vaccine introduction: a multicenter, prospective observational study in three areas of Japan.
Abstract In Japan, monovalent and pentavalent rotavirus (RV) vaccines were approved in 2011 and 2012, respectively. To monitor the changes in the RV genotypes before and after vaccine introduction, we performed a prospective observational study among children (
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Tanaka T, Kamiya H, Asada K, Suga S, Ido M, Umemoto M, Ouchi K, Ito H, Kuroki H, Nakano T, Taniguchi K Tags: Jpn J Infect Dis Source Type: research

Two Siblings with Abnormal Findings of Head MRI Born to an HIV-positive Woman.
PMID: 28250255 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Tsuzuki S, Hosokawa S, Matsushita T Tags: Jpn J Infect Dis Source Type: research

Antimicrobial resistance and molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from child patients of high-risk wards in Shenzhen, China.
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) causes high rates of mortality and a substantial burden to health systems worldwide. Here, we investigated the antimicrobial susceptibility and molecular characteristics of MRSA isolated from children treated at Shenzhen Children's Hospital. 140 MRSA strains were characterized by antimicrobial susceptibility testing. We further performed spa typing, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec), pvl gene and pulsed-field gel electrophoresis (PFGE). These MRSA strains were sensitive to most non-β-lactam antimicrobi...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Qin Y, Wen F, Zheng Y, Zhao R, Hu Q, Zhang R Tags: Jpn J Infect Dis Source Type: research

Clinical study of new tetravalent (type A, B, E, and F) botulinum toxoid vaccine derived from M toxin in Japan.
This study indicated that the vaccine has marked immunogenicity and safety for humans. PMID: 28250257 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Torii Y, Sugimoto N, Kohda T, Kozaki S, Morokuma K, Horikawa Y, Ginnaga A, Yamamoto A, Takahashi M Tags: Jpn J Infect Dis Source Type: research

Molecular detection and characterization of Anaplasma species in wild deer and boars in Gifu Prefecture, Japan.
PMID: 28250258 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Matsuo K, Moribe J, Abe N Tags: Jpn J Infect Dis Source Type: research

A case of congenital folliculitis caused by Pseudomonas aeruginosa in a preterm neonate.
Abstract Intrauterine infections are associated with life-threatening neonatal conditions such as sepsis, intracranial hemorrhage, and chronic lung disease. Herein we present a case of generalized congenital folliculitis caused by Pseudomonas aeruginosa (P. aeruginosa) in a preterm neonate of 27 weeks gestational age successfully treated with antibiotics. Folliculitis is an important manifestation of intrauterine P. aeruginosa infection and prompt, effective treatment is crucial to ensuring a good prognosis. PMID: 28250259 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Matsui K, Okazaki K, Horikoshi Y, Kakinuma R, Kondo M Tags: Jpn J Infect Dis Source Type: research

Burden of rotavirus and enteric bacterial pathogens among children under five years old hospitalized with diarrhea in suburban and rural areas in Kenya.
This study aimed to investigate the incidence of rotavirus and enteric bacterial infections among children up to five years old with diarrhea living in suburban and rural areas in Kenya. It was a cross-sectional descriptive study, and a total of 1060 diarrheal fecal specimens were obtained from 722 children at the Kiambu County Hospital (KCH) located in a suburban area and 338 children from the Mbita District Hospital (MDH) located in a rural part of western Kenya, between August 2011 and December 2013. Of the 1060 isolates, group A rotavirus (RVA) was detected in 29.6% (214/722) and 11.3% (38/338) fecal specimens from KCH...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Shah M, Odoyo E, Wandera E, Kathiiko C, Bundi M, Miringu G, Guyo S, Komoto S, Nyangao J, Karama M, Tsuji T, Taniguchi K, Morita K, Ichinose Y Tags: Jpn J Infect Dis Source Type: research

High prevalence of Campylobacter ureolyticus in stool specimens of children with diarrhea in Japan.
In this study, a total 586 stool samples were collected from 0-12 year-old children with diarrhea between November 2013 and April 2015 and examined with microbiological tests in the hospital for diagnosis of common enteric pathogens including C. jejuni and C. coli. Then in our laboratory, these samples were analyzed by 16S rRNA sequence-based Campylobacter genus-specific PCR (C16S PCR) and 283 (48.3%) samples show positive with this PCR assay. Furthermore, C. ureolyticus was screened in these 283 samples by PCR assay, which can detect this species specifically. Surprisingly, C. ureolyticus was detected in 147 out of the 28...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Hatanaka N, Shimizu A, Somroop S, Li Y, Asakura M, Nagita A, Awasthi SP, Hinenoya A, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Increased irisin concentrations in patients with crimean-congo hemorragic fever.
INCREASED IRISIN CONCENTRATIONS IN PATIENTS WITH CRIMEAN-CONGO HEMORRAGIC FEVER. Jpn J Infect Dis. 2017 Feb 28;: Authors: Büyüktuna SA, Doğan HO, Bakır D, Erşan S, Koç D, Bakır S Abstract Crimean-Congo Hemorrhagic Fever (CCHF) is a life-threatening viral infection. The pathogenesis of the disease is not well understood. The aim of this study was to determine the change in irisin concentrations in patients with CCHF. The study included a total of 30 patients with CCHF and 30 control subjects. Irisin concentrations were determined using a commercial ELISA kit. Median irisin concentr...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Büyüktuna SA, Doğan HO, Bakır D, Erşan S, Koç D, Bakır S Tags: Jpn J Infect Dis Source Type: research

Lambda-Interferons inhibit Herpes Simplex Virus Type 2 Replication in Human Cervical Epithelial Cells through Activation of JAK/STAT Pathway.
In this study, we demonstrated that IFN-λs could potently inhibit HSV-2 replication without cytotoxicity. Investigation of the mechanism(s) showed that IFN-λs induced expression of IFN stimulated genes (ISGs) and also enhanced the expression of several pattern recognition receptors (PRRs). Among these IFN-λs members, IFN-λ3 induced higher levels of ISGs and PRRs expression. In addition, IFN-λs upregulated a number of genes for components of the Janus kinase signal transducers and activators of transcription (JAK/STAT) signaling pathway. Inhibition of JAK/STAT signaling pathway by a JAK in...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Li Z, Lu X, Zhu Y, Cheng P, Liu S, Zhang Y, Tang J, Yang S, Zhou L Tags: Jpn J Infect Dis Source Type: research

Methicillin susceptible teicoplanin resistant Staphylococcus haemolyticus from bloodstream infection: Novel mutation in teicoplanin resistant operon (tcaRAB).
Abstract Staphylococcus haemolyticus is the frequently isolated coagulase-negative staphylococci (CoNS) from blood culture. Here we port a case of methicillin-susceptible S. haemolyticus resistant to teicoplanin and heteroresistant to vancomycin. The isolate was found to susceptible to cefoxitin and resistant to teicoplanin in E-test. PAP-AUC analysis confirmed the presence of vancomycin heteroresistant subpopulation. NGS analysis of the genome revealed the presence of blaZ, msr(A) codes for cross-resistance of macrolide, lincosamide and streptogramin B and quinolone resistance encoding norA gene. Amino acid subst...
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Bakthavatchalam YD, David Sudarsanam T, Babu P, Munuswamy E, Muthuirulandi Sethuvel DP, Devanga Ragupathi NK, Veeraraghavan B Tags: Jpn J Infect Dis Source Type: research

Peripheral blood plasmacytosis in severe fever with thrombocytopenia syndrome.
PMID: 28250265 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 28, 2017 Category: Infectious Diseases Authors: Wada T, Iwata Y, Kamikawa Y, Wada T, Yachie A Tags: Jpn J Infect Dis Source Type: research

Acute Encephalitic Syndrome in Adults and its Correlation with Cytokine Levels in Serum and Cerebrospinal Fluid.
In this study, we assessed the serum and cerebrospinal fluid (CSF) levels of cytokines and their correlation with clinical symptoms. Patients with AES of less than 2 weeks duration underwent brain imaging followed by a CSF examination for routine parameters and viral studies. Cytokines IL-6, IL-10 and RANTES levels were assessed in the serum in all patients and in 50 CSF samples and compared with serum cytokine levels in 64 age and sex matched controls. Of the 87 AES patients, 13 had Japanese Encephalitis. Serum IL-6, IL-10 and RANTES were significantly elevated in patients of AES compared to control group. Serum IL-10 was...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Singh SK, Kulshreshtha D, Singh AK, Maurya PK, Thacker AK Tags: Jpn J Infect Dis Source Type: research

Multicenter study of the clinical presentation of Staphylococcus lugdunensis bacteremia in Japan.
In this study, SL resulted in the development of bacteremia in select patients. Clinicians in Japan should be aware of the prevalence of SL and the complications of SL bacteremia. PMID: 28003590 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Ainoda Y, Takeshita N, Hase R, Mikawa T, Hosokawa N, Kawamura I, Kurai H, Abe M, Kimura M, Araoka H, Fujita T, Totsuka K, Mezaki K, Sekiya N, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Comparison of enterohemorrhagic Escherichia coli (EHEC) O157 and EHEC non-O157 isolates from diarrheal patients in the Republic of Korea.
Abstract We compared 47 enterohemorrhagic Escherichia coli (EHEC) O157 isolates with 184 EHEC non-O157 isolates from Korean patients with diarrhea. In the O157 group, the strains harboring both shiga toxin genes (stx1 and stx2) were detected with highest frequency, whereas the strains harboring only stx1 gene were most frequently detected in the non-O157 group. Eight virulence genes (eaeA, hlyA, ehx, iha, efa1, tir, toxB, and espA ) were found to show a higher frequency of occurrence in the O157 group than in the non-O157 group. In addition, the symptom of bloody diarrhea was found with a higher occurrence in the ...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Oh KH, Jung SM, Shin E, Chung GT, Seong WK, Cho SH Tags: Jpn J Infect Dis Source Type: research

Antibiotic susceptibilities and genetic characterization of extended spectrum beta-lactamase producing Escherichia coli isolated from stools of pediatric diarrhea patients in Surabaya, Indonesia.
Abstract The purpose of this study was to investigate extended-spectrum beta-lactamase (ESBL)-producing E. coli isolated from pediatric (0-3 year old) diarrhea patients, as well as bacterial antibiotic susceptibilities, ESBL typing, multilocus sequence typing (MLST) typing and diarrheagenic E. coli (DEC)-typing in Surabaya, Indonesia, where this kind of survey is rarely done. ESBL-producing E. coli were detected in 18.8 % of strains. Many ESBL-producing E. coli had significantly less susceptibility to gentamicin (p
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Wasito EB, Shigemura K, Osawa K, Fardah A, Kanaida A, Raharjo D, Kuntaman K, Hadi U, Harijono S, Sudarmo SM, Nakamura T, Shibayama K, Fujisawa M, Shirakawa T Tags: Jpn J Infect Dis Source Type: research

Dengue virus-induced reactive oxygen species production in rat microglia cells.
In conclusion, DV2 and DV4 have the capability to induce ROS production and activate microglia, which have been reported as the key components for neuronal damage. PMID: 28003593 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Suwanprinya L, Morales NP, Sanvarinda P, Dieng H, Okabayashi T, Vargas RE Tags: Jpn J Infect Dis Source Type: research

First Isolation of Hybrid Shigatoxigenic and Enterotoxigenic Escherichia coli (STEC/ETEC) Strain Harboring stx2 and elt Genes in Republic of Korea.
PMID: 28003594 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Oh KH, Shin E, Jung SM, Im J, Cho SH, Hong S, Yoo CK, Chung GT Tags: Jpn J Infect Dis Source Type: research

Detection of measles virus genotypes B3, D4, D5, D8, and H1 in the surveillance system in Hokkaido, Japan, during pre- and post-elimination Era, 2006-2015.
Abstract Measles is an acute and highly contagious disease caused by measles virus (MeV). In Japan, since the last epidemic in 2007 and 2008 caused by the genotype D5 strains, the government introduced a catch-up-vaccination program for teenagers during fiscal years of Japan 2008-2012 and the mandatory case-based reporting system for the nationwide elimination. Furthermore, the laboratory confirmation based on genotyping has been performed to clarify the source of infection and to support the interruption of measles cases. While such preventive measure have been conducted, the number of measles cases decreased ste...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Miyoshi M, Komagome R, Yamaguchi H, Ohnishi A, Kikuchi M, Ishida S, Nagano H, Okano M Tags: Jpn J Infect Dis Source Type: research

A case of rat bite fever caused by Streptobacillus moniliformis.
We report a case of rat bite fever (RBF) diagnosed by detection of Streptobacillus moniliformis from blood culture and synovial fluid. The patient was a 45-year-old man with alcoholic liver cirrhosis. He was bitten on his third finger by a rat, which was caught in a mousetrap installed in his house. After the bite, fever, rash and arthralgia developed over a period of about two weeks. The patient was admitted to our hospital and treated with combination of Ampicillin- Sulbactam (SAM), Vancomycin (VAN) and Minocycline (MIN). The Anaerobic/F resin blood culture bottle became positive with gram negative bacillus in overnight ...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Hayakawa Y, Suzuki J, Suzuki M, Sugiura W, Ohkusu K Tags: Jpn J Infect Dis Source Type: research

Safety and Immunogenicity Study of a 9-Valent Human Papillomavirus Vaccine Administered to 9-To-15 Year-Old Japanese Girls.
We report here the results of an open-label phase III study conducted to bridge the findings in women age 16 to 26 years to Japanese girls aged 9 to 15 years. All subjects (n = 100) received a 3-dose regimen of 9vHPV vaccine at day 1, month 2 and month 6. Anti-HPV serologic assays were performed at day 1, month 7, month 12, month 24 and month 30. At month 7 (4 weeks after dose 3), 100% of subjects seroconverted for each vaccine HPV type. Increases in geometric mean titers of anti-HPV 6/11/16/18/31/33/45/52/58 in girls were similar to those in Japanese women aged 16 to 26 years in the pivotal phase III study. Persistence of...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Iwata S, Murata S, Han SR, Wakana A, Sawata M, Tanaka Y Tags: Jpn J Infect Dis Source Type: research

An iTRAQ-based quantitative proteomics study of refractory mycoplasma pneumoniae pneumonia patients.
In this study, we applied the quantitative proteomic technique(iTRAQ) to globally analyze MP-related proteins in serum samples from five RMPP and five non-refractory MP pneumonia(NRMPP) patients, and five healthy children. Functional classification, sub-cellular localization, and protein interaction network were carried out based on PANTHER and cytoscape analysis. A total of 260 differentially expressed proteins were identified in the RMPP and NRMPP groups. Compared with the control group, there were 134(70 up-regulated and 64 down-regulated) and 126(63 up-regulated and 63 down-regulated) differentially expressed proteins ...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Yu JL, Song QF, Xie ZW, Jiang WH, Chen JH, Fan HF, Xie YP, Lu G Tags: Jpn J Infect Dis Source Type: research

Establishment of Subclones of Severe Fever with Thrombocytopenia Syndrome Virus YG1 Strain Selected by the Degree of Low-pH-dependent Cell Fusion Activity.
Abstract Severe fever with thrombocytopenia syndrome virus YG1 strain was isolated from the first clinical case in Japan. We found that only some of the cells underwent low-pH-dependent cell fusion, though all of the cells were confirmed to have been infected with viruses, suggesting that YG1 strain consists of heterogeneous mixtures of related viruses. Here, we established three subclones, E3, A4 and B7, from YG1 strain by the limiting dilution method with pH-dependent cell fusion activities. Subclone E3 showed weak fusion activity and cytopathic effect (CPE) in Vero E6 cells. It has an amino acid sequence identi...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Nishio S, Tsuda Y, Ito R, Shimizu K, Yoshimatsu K, Arikawa J Tags: Jpn J Infect Dis Source Type: research

Identification of Group G Streptococci Isolates from Companion Animals in Japan and Their Antimicrobial Resistance.
This study determined species-level identification and antimicrobial resistance (AMR) properties in group G streptococci (GGS) isolates from companion animals in Japan. Strains were isolated from sterile/non-sterile specimens of 72 animals with clinical symptoms or signs in April-May, 2015. We identified them based on 16S rRNA sequencing, mass spectrometry (MS), and an automated method based on their biochemical properties. Antimicrobial susceptibility was determined with broth microdilution method and Etest. AMR determinants [erm(A), erm(B), mef(A), tet(M), tet(O), tet(K), tet(L), and tet(S)] were amplified by polymerase ...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Tsuyuki Y, Kurita G, Murata Y, Goto M, Takahashi T Tags: Jpn J Infect Dis Source Type: research

Molecular epidemiological study of mumps epidemics of 2015 in Okinawa, Japan.
Abstract In Okinawa Prefecture in Japan, although major mumps epidemics have occurred every 4-5 years, no laboratory diagnoses have been conducted. The mumps epidemic spread in Okinawa from October 2014, and we collected clinical materials from 31 mumps patients in 4 areas (Hokubu, Nanbu, Miyako and Yaeyama) from July to December 2015 for virus isolation and RT-PCR, whose positive ratios were 52% and 87%, respectively. The phylogenetic analyses showed that all isolates were classified into genotype G, most of which, except for 1, consisted of two subgenotypes, Ge (55.6%) and Gw (40.7%), which have been prominent i...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Kuba Y, Kyan H, Arakaki E, Takara T, Kato T, Okano S, Oshiro Y, Kudaka J, Kidokoro M Tags: Jpn J Infect Dis Source Type: research

Evaluation of Immunochromatography Tests and New Enzyme Immunoassay for Detection of Novel GII.17 Norovirus in Stool Samples.
In this study, the sensitivity of seven brands of immunochromatography (IC) test kits, 6 are on the market in Japan and 1 in Europe, were evaluated by testing with confirmed GII.17 NoV positive stool samples. The stool samples were also tested by bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR was used as a gold standard method. Among seven brands IC kits, RIDA QUICK, was the most sensitive kit with the detection rate of 10(7) copies/ml while those of other IC kits ranged from 10(8)-10(9) copies/ml. Nevertheless, it should be pointed out that the detection rate of BLEIA was about 100-1,000 times (10(4)-10(5) co...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Ushijima H, Thongprachum A, Khamrin P, Takanashi S, Okitsu S, Maneekarn N, Hayakawa S Tags: Jpn J Infect Dis Source Type: research

A Single Vaccination of Nonhuman Primates with Highly Attenuated Smallpox Vaccine, LC16m8, Provides Long-term Protection against Monkeypox.
Abstract Monkeypox virus (MPXV) causes human monkeypox (human MPX), which is a similar disease with smallpox in humans. A previous study showed that a single vaccination of monkeys with LC16m8, a highly attenuated smallpox vaccine, protected them from MPX at 4-5 weeks after vaccination. In the present study, we evaluated the long-term efficacy of a single vaccination with LC16m8 in a nonhuman primate model of MPXV infection. The monkeys were inoculated with LC16m8, Lister (parental strain of LC16m8), or mock and then challenged with MPXV via the subcutaneous route at 6 and 12 months after vaccination, which we com...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Iizuka I, Ami Y, Suzaki Y, Nagata N, Fukushi S, Ogata M, Morikawa S, Hasegawa H, Mizuguchi M, Kurane I, Saijo M Tags: Jpn J Infect Dis Source Type: research

Genetic characterization of rare genotype GII.5 sapovirus strain detected from food-borne suspected gastroenteritis outbreak among adults in Japan, 2010.
PMID: 28003604 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Oka T, Doan YH, Haga K, Mori K, Ogawa T, Yamazaki A Tags: Jpn J Infect Dis Source Type: research

Contribution of Streptococcus gordonii Hsa adhesin to biofilm formation.
Abstract Adhesion of oral mitis group streptococci such as Streptococcus gordonii to acquired pellicle of the tooth surface is the initial step of oral biofilm formation. S. gordonii wild type strain DL1 possesses Hsa adhesin, recognizing terminal sialic acid of the host sialoglycoconjugates. The aim of the present study was to investigate the role of Hsa adhesin in biofilm formation. Biofilm-forming ability of the S. gordonii hsa mutant in microtiter plates pre-coated with saliva, fetuin and mucin was significantly reduced, compared to that of DL1. In contrast, no significant difference in biofilm-forming ability...
Source: Japanese Journal of Infectious Diseases - December 22, 2016 Category: Infectious Diseases Authors: Oguchi R, Takahashi Y, Shimazu K, Urano-Tashiro Y, Kawarai T, Konishi K, Karibe H Tags: Jpn J Infect Dis Source Type: research

Pseudomonas aeruginosa Bacteremia among Immunocompetent and Immunocompromised Patients: Relation to Initial Antibiotic Therapy and Survival.
Abstract Pseudomonas aeruginosa bacteremia occurs mainly in immunocompromised patients. However, P. aeruginosa bacteremia in immunocompetent patients has also been reported. The aim of this study was to evaluate the clinical characteristics of P. aeruginosa bacteremia in relation to the immune status of the patients. The medical records of 126 adult patients with P. aeruginosa bacteremia in Nagasaki University Hospital were retrospectively reviewed between January 2003 and December 2012. Of 126 patients with P. aeruginosa bacteremia, 60 patients (47.6%) were classified as immunocompetent. Mortality in immunocompet...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Migiyama Y, Yanagihara K, Kaku N, Harada Y, Yamada K, Nagaoka K, Morinaga Y, Akamatsu N, Matsuda J, Izumikawa K, Kohrogi H, Kohno S Tags: Jpn J Infect Dis Source Type: research

Geo-Demography of HIV/AIDS in Japan from 1985 to 2011: Incidence and Transmission Mode under Influence of Population Size/Density.
Abstract A stable relation was found between number of HIV/AIDS patients (P) and population size (N) and between HIV/AIDS incidence (I) and population density (D). The relation could be expressed as P = kN(m) or I = hD(n), where k, h, m, and n are constants. For "AIDS"/"AIDS diagnosis", the constant m was 1.5 for Japan and 1.3 for the United States of America (USA); n was 0.38 for both Japan and the USA. These observations indicated that larger population sizes related to disproportionately larger numbers of HIV/AIDS patients, and denser populations had disproportionately higher incidences of H...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Sporogonic Cycles Calculated Using Degree-Days, as a Basis for Comparison of Malaria Parasite Development in Different Eco-Epidemiological Settings in India.
Abstract In India, malaria transmission is prevalent across diverse geologies and ecologies. Temperature is one of the key determinants of malarial transmission, causing low endemicity in some areas than in others. Using a degree-day model, we estimated the maximum and minimum possible number of days needed to complete a malarial sporogonic cycle (SC), in addition to the possible number of SCs for Plasmodium vivax and Plasmodium falciparum under two different ecological settings with either low or high endemicity for malaria at different elevations. In Raikhalkhatta (in the Himalayan foothills) SCs were modeled as...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Singh P, Dhiman RC Tags: Jpn J Infect Dis Source Type: research

Field and Laboratory Evaluations of the Efficacy of DEET Repellent against Ixodes Ticks.
The objective of this study was to clarify the efficacy of a currently available N,N-diethyl-m-toluamide (DEET) repellent against tick species in Japan. We performed 2 different field trials: "human trap," and "flag-dragging." In total, 482 ticks were collected from white flannel cloths in the field studies. The collected tick species were Ixodes persulcatus and I. ovatus, which accounted for 5.3% and 94.7% of the ticks in the human trap test and 31.4% and 68.6% in the flag-dragging test, respectively. The repellency levels of DEET-treated flannel cloths in the human trap and flag-dragging tests were 84...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Ogawa K, Komagata O, Hayashi T, Itokawa K, Morikawa S, Sawabe K, Tomita T Tags: Jpn J Infect Dis Source Type: research

A Epidemiological Investigation of a Nosocomial Outbreak of Multidrug-Resistant Acinetobacter baumannii in a Critical Care Center in Japan, 2011-2012.
Abstract In 2011, a multidrug-resistant Acinetobacter baumannii (MDRAB) outbreak occurred at a Japanese critical care center (CCC) in a tertiary care hospital. Multidrug-resistance in Japan is defined as resistance to the antimicrobials amikacin, carbapenem, and fluoroquinolone. We conducted a retrospective epidemiological investigation of this outbreak to identify the risk factors for MDRAB respiratory tract acquisition in this hospital. Cases were defined as hospitalized patients with MDRAB-positive cultures at least 3 days post admission to the CCC between June 1, 2011 and April 20, 2012. Fifteen MDRAB cases we...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Ushizawa H, Yahata Y, Endo T, Iwashima T, Misawa M, Sonobe M, Yamagishi T, Kamiya H, Nakashima K, Matsui T, Matsui M, Suzuki S, Shibayama K, Doi M, Irie F, Yamato S, Otomo Y, Oishi K Tags: Jpn J Infect Dis Source Type: research

Risk Factors for Colistin-Associated Acute Kidney Injury: A Multicenter Study from Turkey.
Abstract The aim of this study was to investigate the incidence of acute kidney injury (AKI) and risk factors due to colistin use in patients infected with multidrug-resistant pathogens. This multicenter, retrospective, observational study was conducted in Turkey, at 5 different research and university hospitals. Cox regression analyses were performed, to determine independent predictors of AKI. From April 2012 to July 2014, a total of 216 patients aged between 18-94 years, treated with colistimethate sodium (CMS) were included in the study. The mean age of the patients was 60.3 ± 20.1 years. The overall in...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Gul S, Kuscu F, Aydemir H, Ozturk DB, Deveci O, Duygu F, Kacmaz B, Yaman F, Aslan E Tags: Jpn J Infect Dis Source Type: research

In Vivo Efficacy of Drugs against Toxoplasma gondii Combined with Immunomodulators.
k M Abstract The aim of this study is to evaluate the effects of pyrimethamine (PYR) and sulfadiazine (SDZ) combined with levamisole and echinacea on the survival of mice infected with Toxoplasma gondii. For this, we used 99 specific pathogen-free BALB/c mice. All the mice were infected intraperitoneally with 10(5) T. gondii tachyzoites and were divided into 11 groups, each including 9 mice. Except for the control group, oral treatment was initiated in all groups 24 h post infection and was continued for 10 days. The treatment regimen included dual combinations of PYR (dose, 6.25 and 12.5 mg/kg/day) and SDZ (dose,...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Köksal ZŞ, Yanik K, Bilgin K, Yılmaz EM, Hokelek M Tags: Jpn J Infect Dis Source Type: research

Pathophysiological Implication of Computed Tomography Images of Chronic Pulmonary Aspergillosis.
Abstract Chronic pulmonary aspergillosis (CPA) is a refractory disorder that needs long-term antifungal treatment and occasionally results in fatal respiratory failure and hemoptysis. However, the pathological features of the disorder have not been thoroughly delineated. Thirty cases were therefore analyzed clinically and histologically to elucidate the pathophysiology of CPA. The subjects comprised 14 individuals who underwent surgical removal and 16 patients who died. No subject exhibited a severely immunocompromised state. The main symptoms included cough, hemosputum, and dyspnea. Chest computed tomography (CT)...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Ando T, Tochigi N, Gocho K, Moriya A, Ikushima S, Kumasaka T, Takemura T, Shibuya K Tags: Jpn J Infect Dis Source Type: research

Chikungunya Fever in Japan Imported from the Caribbean Islands.
Abstract A 53-year-old Japanese woman who was working as a volunteer in the Commonwealth of Dominica in the Caribbean islands presented with a high-grade fever and severe incapacitating generalized arthralgia. The Asian genotype of the chikungunya virus was confirmed using reverse transcription-PCR and serology, based on the presence of a specific neutralization titer and immunoglobulin M antibodies. She was diagnosed with post-chikungunya chronic arthritis based on persistence of her polyarthritis for 3 months and the presence of rheumatoid factor, immunoglobulin G-rheumatoid factor, and matrix metalloproteinase-...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Imai K, Nakayama E, Maeda T, Mikita K, Kobayashi Y, Mitarai A, Honma Y, Miyake S, Kaku K, Miyahira Y, Kawana A Tags: Jpn J Infect Dis Source Type: research

Clonal Spread of Enterotoxigenic Escherichia coli O128:H45 Strain in the Neonate Unit.
Abstract Enterotoxigenic Escherichia coli (ETEC) has the potential to cause nosocomial infantile diarrhea in a hospital setting. We detected 12 ETEC serotype O128:H45 isolates from diarrheal neonates in our neonatal unit from July through October 2012. These infants developed hospital-acquired and epidemiologically related diarrhea. Pulsed-field gel electrophoresis analysis and multilocus sequence typing of these 12 isolates suggested that a specific clone of ETEC serotype O128:H45-CS21-ST2332 caused nosocomial diarrhea among neonates. Of concern, this ETEC clone strain was resistant to multiple drugs, particularl...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Zeng M, Shi W, Chang H, Wang A, He L, Fu P, Xu X, Wang C Tags: Jpn J Infect Dis Source Type: research

Nontuberculous Mycobacterial Osteomyelitis in Human Immunodeficiency Virus-Negative Patients: A Case Series.
Abstract Nontuberculous mycobacterial bone infections among human immunodeficiency virus (HIV)-negative patients are rare, although a few studies have described such infections. We retrospectively reviewed the medical and microbiological data of HIV-negative osteomyelitis cases caused by nontuberculous mycobacteria treated in our tertiary-care hospital over 14 years from January 1, 2000, to December 31, 2013. Three HIV-negative patients had contracted bone infections due to nontuberculous mycobacteria. All of the patients had at least 1 predisposing condition that led to the infections: idiopathic CD4-positive lym...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Kobayashi T, Morino E, Takasaki J, Nagahara Y, Sugiyama H Tags: Jpn J Infect Dis Source Type: research

Pentagalloylglucose Blocks the Nuclear Transport and the Process of Nucleocapsid Egress to Inhibit HSV-1 Infection.
In this study, we found that PGG treatment delays the nuclear transport process of HSV-1 particles by inhibiting the upregulation of dynein (a cellular major motor protein) induced by HSV-1 infection. Furthermore, PGG treatment affects the nucleocapsid egress of HSV-1 by inhibiting the expression and disrupting the cellular localization of pEGFP-UL31 and pEGFP-UL34, which are indispensable for HSV-1 nucleocapsid egress from the nucleus. However, the over-expression of pEGFP-UL31 and pEGFP-UL34 could decrease the antiviral effect of PGG. In this study, for the first time, the antiviral activity of PGG against acyclovir-resi...
Source: Japanese Journal of Infectious Diseases - December 15, 2016 Category: Infectious Diseases Authors: Jin F, Ma K, Chen M, Zou M, Wu Y, Li F, Wang Y Tags: Jpn J Infect Dis Source Type: research