Epidemiological survey of Babesia divergens Asia lineage in wild sika deer (Cervus nippon) by using direct PCR, in Japan.
Abstract Babesia divergens is the major causal agent of zoonotic human babesiosis in Europe. In Japan, a parasite genetically closely related to the European B. divergens, B. divergens Asia lineage, was detected in wild sika deer (Cervus nippon) in our previous study. We therefore conducted large epidemiological survey by combining lineage specific PCR system and blood direct PCR. The infection rate of the Asia lineage was 6.6% (116/1747) throughout the country, where Hokkaido (45%), Nagano (17%), Iwate (12%), Gunma (11%) and Yamanashi (11%) were highly enzootic (>10%) among 30 prefectures examined. European B....
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Zamoto-Niikura A, Hagiwara K, Imaoka K, Morikawa S, Ishihara C, Hanaki KI Tags: Jpn J Infect Dis Source Type: research

Detection of Borrelia DNA in tick species collected from vegetation and wild animals in Fukuoka, Japan.
Abstract We surveyed the presence of Borrelia spp. in ticks collected from vegetation by flagging and from wild animals from May 2017 to November 2018 in Fukuoka, located in the northern Kyushu area of Japan. A total of 1,601 ticks were collected and morphologically separated into 9 species: Ixodes turdus, I. ovatus, Amblyomma testudinarium, Haemaphysalis flava, H. formosensis, H. kitaokai, H. longicornis, H. hystricis, and H. megaspinosa. The ticks were segregated into 561 pools and nested PCR was used to detect borrelial DNA. Borrelia turdi and Borrelia sp. HM were identified in two of the 561 pools. This is the...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Nakayama S, Kobayashi T, Nakamura A, Yoshitomi H, Song Y, Ashizuka Y Tags: Jpn J Infect Dis Source Type: research

Abdominal Aortic Graft Infection Caused by stG485.0, ST29 Streptococcus dysgalactiae subsp. equisimilis.
Abstract In recent years, the prevalence of invasive Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections has increased gradually throughout the world, including Japan. Here, we report the case of an abdominal aortic graft infection caused by stG485.0, ST29 SDSE in an elderly patient with diabetes. An 86-year-old man who had undergone an abdominal aortic aneurysm (not infected) repair with a bifurcated graft 10 years ago was referred to our hospital after being suspected of having abdominal aortic graft infection through computed tomography. He underwent surgery, and the pus developed between the aneury...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Kakuta R, Shimizu T, Goto H, Endo S, Kanamori H, Ohta N, Nakano R, Yano H, Katori Y, Kaku M Tags: Jpn J Infect Dis Source Type: research

Molecular identification of the etiologic agent of human gnathostomosis in an endemic area of Mexico.
This study aims to develop a duplex-PCR method of the ITS-2 region for differentiating G. binucleatum and G. turgidum parasites that coexist in the same endemic area and to identify the Gnathostoma larvae recovered from biopsies of two gnathostomosis patients from Sinaloa, Mexico. The duplex PCR established based on the ITS-2 sequence showed that the length of the amplicons was 321 bp for G. binucleatum and 226 bp for G. turgidum. The amplicons from the AL3A of both patients were 321 bp. Furthermore, the length and composition of these amplicons were identical to that deposited in GenBank as G. binucleatum (accession No. J...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Díaz-Camacho SP, Parra-Unda JR, Ríos-Sicairos J, Delgado-Vargas F Tags: Jpn J Infect Dis Source Type: research

Molecular characteristics of novel mono-reassortant G9P[8] rotavirus A strains possessing the NSP4 gene of the E2 genotype detected in Tokyo, Japan.
Abstract Rotavirus A (RVA) has been detected from patients with gastroenteritis even after vaccine introduction in Japan. To investigate the circulating RVA strains, the RVA-positive stool specimens obtained in Tokyo in 2017 and 2018 were analyzed by next-generation sequencing. A total of 50 and 21 RVA samples were obtained in 2017 and 2018, respectively. In 2017, G2P[4] (40.0%) was the most prevalent strain, followed by G3P[8] (DS-1-like) (28.0%), G8P[8] (10.0%), G3P[8] (Wa-like) (8.0%), G9P[8]-E1 (8.0%), and mixed infection (6.0%). In 2018, G3P[8] (DS-1-like) (28.6%) and G9P[8]-E2 (28.6%) were the prevalent stra...
Source: Japanese Journal of Infectious Diseases - September 30, 2019 Category: Infectious Diseases Authors: Fujii Y, Oda M, Somura Y, Shinkai T Tags: Jpn J Infect Dis Source Type: research

Comparison of Haemophilus influenzae Seroprevalence in Serum Samples Collected from 0-5-year-old Children in Japan in 1980, 1995, 2010, and 2012.
Abstract Haemophilus influenzae type b (Hib) causes various invasive infections, such as meningitis, septic arthritis, and pneumonia, especially in children under 5 years of age. Despite Hib vaccines protect Hib infection, seroepidemiological surveys of Hib infections have not been conducted systematically in Japan thus far. We analyzed 1,338 serum samples from 0-5-year-old children that were provided by the National Serum Reference Bank of the National Institute of Infectious Diseases (Tokyo, Japan). Serum levels of the anti-polyribosylribitol phosphate (PRP) IgG antibody against the Hib were determined using an ...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Arai S, Satoh H, Okuno H, Morino S, Oishi K, Tanaka-Taya K Tags: Jpn J Infect Dis Source Type: research

Antiviral and Virucidal Activities of Umesu Phenolics on Influenza Viruses.
Abstract Umesu phenolics were purified from the salt-extracts of Japanese apricot (Nanko-mume cultivar of Prunus mume Sieb. et Zucc.). Characterization of umesu phenolics revealed that they inhibited the multiplication of influenza virus and several other RNA and DNA viruses, when added to the culture media of the infected cells. In addition to these antiviral activities, the phenolics significantly decreased a plating efficiency of influenza virus, if present in the virus inoculum. More drastic effects were observed in virucidal activity; the infectivity of several strains of influenza viruses decreased less than...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Ikeda K, Nishide M, Tsujimoto K, Nagashima S, Kuwahara T, Mitani T, Koyama AH Tags: Jpn J Infect Dis Source Type: research

Comparison of Reverse Transcription Loop-Mediated Isothermal Amplification Method with SYBR Green Real-Time RT-PCR and Direct Fluorescent Antibody Test for diagnosis of rabies.
This study tried to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of rabies virus RNA in the brain samples in comparison with SYBR Green Real Time RT-PCR test as a molecular technique and Direct Fluorescent Antibody Test as a serologic method. In this study, RT-LAMP was developed to rabies diagnosis. Six primers were designed, based on the nucleoprotein (N) of rabies virus. The sensitivity and specificity of SYBR Green Real-Time RT-PCR and RT-LAMP methods were also determined.RT-LAMP optimized at 58˚C for 60 min and the sensitivity and specificity of RT-LAMP an...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Naji E, Fadajan Z, Afshar D, Fazeli M Tags: Jpn J Infect Dis Source Type: research

Prevalence of Virulence Genes of Diarrheagenic Escherichia coli in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh.
Abstract Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and diarrheic patients in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). Invasion-related gene virB had the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB for diffuse adhesion and second with aggR for aggregative adhesion. These three genes were specific to patients. In contrast, Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. eae for attaching and effacing lesion, and elt and est of enterotoxins were detected from all thre...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Parvej MS, Alam MA, Shono M, Zahan MN, Masuma Parvez MM, Karim Ansari W, Jowel MS, Uddin MS, Kage-Nakadai E, Rahman MT, Nishikawa Y Tags: Jpn J Infect Dis Source Type: research

Diversity in Genotype Distribution of Mycoplasma pneumoniae Obtained from Children and Adults.
Abstract The aim of this study was to explore whether there was any special genotype responsible for the high prevalence of Mycoplasma pneumoniae (M.pneumoniae) infection in children. A total of 247 M. pneumoniae - DNA positive clinical specimens including 200 from children and 47 from adults collected in Beijing, China, during the same period were analyzed. We performed P1-restriction fragment length polymorphism analysis (RFLP), multi-locus variable number tandem repeat analysis (MLVA) and detected the macrolide resistance-associated mutations in 23S rRNA of the clinical specimens. In the present study, we obser...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Yan C, Yang H, Sun H, Zhao H, Feng Y, Xue G, Li S, Cui J, Ni S Tags: Jpn J Infect Dis Source Type: research

Fluctuations in antibody titers against enterovirus D68 in pediatric sera collected in a community before, during, and after a possible outbreak.
Abstract Previously, we reported a hospital-based epidemiological study on enterovirus (EV)-D68 infection among children during the autumn of 2015, which indirectly inferred an outbreak in Sendai, Japan. In the present study, we collected sera from children (aged 0-6 years; without symptoms of infectious diseases) in the Sendai community during four sample collection periods (1 year before, 6 months before, immediately after, and 1 year after the possible outbreak period). Immunity levels against EV-D68 infection in the children were analyzed by assaying the neutralization antibody titers of the sera. Further, it ...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Kadji FMN, Nishimura H, Okamoto M, Sato K, Ohmiya S, Ito H, Suzuki A, Nagai Y, Oshitani H Tags: Jpn J Infect Dis Source Type: research

Two cases of dengue virus type 2 (DENV-2) infection in a Japanese couple returning from Maldives in the dengue outbreak in 2018.
Abstract Annually, more than 1.2 million travelers from other countries visit Maldives for sightseeing, business and honeymoons. In 2018, the largest dengue fever outbreak occurred and more than 3,200 people were affected. During this outbreak, we encountered a newly married Japanese couple returning from Maldives on their honeymoon in October 2018, both of which got infected by dengue virus type 2 (DENV-2) during the travel. The number of imported dengue fever cases from Maldives may increase, hence, physicians should keep a sharp watch on up-to-date dengue outbreak information all over the world. PMID: 3147...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Mutoh Y, Moriya A, Yasui Y, Saito N, Takasaki T, Hiramatsu S, Izuchi T, Umemura T, Ichihara T Tags: Jpn J Infect Dis Source Type: research

Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation.
kgün M Abstract In addition to tube drains, pleural empyema is treated with antibiotics and anti-inflammatory drugs. We aimed to evaluate the anti-inflammatory activity of roflumilast combined with linezolid in a rat model of pleural empyema induced by Staphylococcus aureus.A total of 40 rats were separated into 7 groups: Sham (n=4), S. aureus inoculation (n=6), S. aureus + 10 mg/kg linezolid (n=6), S. aureus + 5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg linezolid+5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg roflumilast (n=6), and S. aureus + 10 mg/kg linezolid + 10 mg/kg roflumilast (n=6). Linezolid w...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Kerget B, Araz Ö, Kerget F, Erol HS, Özmen S, Halıcı Z, Akgün M Tags: Jpn J Infect Dis Source Type: research

Dengue virus serotype-4 during the 2015-2017 epidemic: emergence of a novel genotype IIa of DENV-4 in the Philippines.
Abstract Dengue remains a major public health problem in the Philippines. Here, we determined the circulating serotypes in the Philippines during the 2015-2017 outbreaks by using a total of 678 serum samples that were collected from 537 individual dengue patients. Following an increase in the percentage of DENV-4 patients in recent years, we conducted a comprehensive molecular epidemiology analysis on the DENV-4 strains isolated recently in the Philippines. While two genotypes of DENV-4 have been isolated in the Philippines since 1956, the GI and GIIa which were the DENV-4 strains isolated in this study were close...
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Luz MAV, Nabeshima T, Moi ML, Dimamay MTA, Pangilinan LS, Dimamay MPS, Matias RR, Mapua CA, Buerano CC, de Guzman F, Tria ES, Natividad FF, Daroy MLG, Takemura T, Hasebe F, Morita K Tags: Jpn J Infect Dis Source Type: research

A quicker Tzanck smear with methylene blue stain in diagnosis of herpesvirus skin infections: a comparative study with Giemsa stain.
PMID: 31474704 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Authors: Wantavornprasert K, Chottawornsak N, Hurst CP, Asawanonda P, Hansasuta P, Rerknimitr P Tags: Jpn J Infect Dis Source Type: research

A Case Report of Cat-to-Human Transmission of Severe Fever with Thrombocytopenia Syndrome Virus.
PMID: 31366857 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kida K, Matsuoka Y, Shimoda T, Matsuoka H, Yamada H, Saito T, Imataki O, Kadowaki N, Noguchi K, Maeda K, Mochizuki Y, Kishimoto T Tags: Jpn J Infect Dis Source Type: research

Conjunctivitis due to human adenovirus type 2 variant identified during epidemic keratoconjunctivitis surveillance in Japan.
PMID: 31366858 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kaneko H, Hanaoka N, Konagaya M, Tsukahara-Kawamura T, Kobayashi M, Nakagawa H, Hatano H, Ikuta K, Fujimoto T Tags: Jpn J Infect Dis Source Type: research

Risk assessment of dengue autochthonous infections in Tokyo in summer, especially in the period of the 2020 Olympic Games.
This study is aiming at analyzing a risk of autochthonous dengue infections in Tokyo in summer and also an additional risk in the Olympiad by using a mathematical model. We developed a stochastic transmission model with the cooperation of seasonal factors which influence much on the transmission cycle of dengue virus, and carried out stochastic simulations for each of the scenarios provided adequately. We found that (1) the incidence of dengue autochthonous infections is predicted to be small number cases; (2) the local climate makes a great influence on a scale of dengue autochthonous infections; (3) the incidence reaches...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Ishikawa H, Shimogawara R Tags: Jpn J Infect Dis Source Type: research

Comparison of in vivo activity of garenoxacin and levofloxacin in a murine pneumonia mixed-infection model by Streptococcus pneumoniae and Parvimonas micra.
Abstract Community-acquired pneumonia (CAP) can involve mixed-infection. However, there are few studies investigating its antimicrobial activity in mixed-infection. The aim of this study was to compare the in vivo antimicrobial activity of GRNX and levofloxacin (LVFX) against Streptococcus pneumoniae and Parvimonas micra in a murine pneumonia mixed-infection model. S. pneumoniae D-6888 and P. micra No. 242 were used in this study. Antimicrobial activity was calculated for each isolate as the change in bacterial count of lungs (Δlog10 CFU/mL) obtained in the treated mice after 24 h compared with the count in ...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Kato H, Hagihara M, Yokoyama Y, Suematsu H, Asai N, Koizumi Y, Yamagishi Y, Mikamo H Tags: Jpn J Infect Dis Source Type: research

High Prevalence of Hepatitis E Virus Infection in Imported Cynomolgus Monkeys in Japan.
Abstract Cynomolgus monkey is an important experimental animal for hepatitis E virus (HEV) infection. In Japan, cynomolgus monkeys are imported mainly from Asian countries for use at animal facilities and institutions. However, the status of HEV infection in cynomolgus monkeys remains unclear. A total of 187 pairs of serum and fecal samples were collected from cynomolgus monkeys (Macaca fascicularis) imported from China and Cambodia to detect anti-HEV IgG and IgM antibodies as well as HEV RNA. Based on an enzyme-linked immunosorbent assay using HEV-like particles derived from genotype 3 HEV as the antigen, 184 of ...
Source: Japanese Journal of Infectious Diseases - July 31, 2019 Category: Infectious Diseases Authors: Zhang W, Yoshizaki S, Ami Y, Suzaki Y, Takeda N, Muramatsu M, Li TC Tags: Jpn J Infect Dis Source Type: research

Higher genetic diversity and viral evolution on key regions of envelope gene are associated with broader neutralizing antibody responses: report of two chronic HIV infected cases.
Abstract Dozens of broadly neutralizing antibodies (bnAbs) have been identified from chronically infected HIV-1 patients, but it is still unclear what determines the acquisition of broad neutralizing activities. Two chronic HIV-1 infected cases with similar autologous neutralizing activities were followed up for two years to study viral evolution of the envelope gene and the neutralizing activity against autologous and heterologous viruses. The neutralization activities against homologous viruses gradually increased in both patients. HA172 eventually developed a cross-clade neutralizing antibodies response, with a...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Shen S, Tian W, Ji Y, Gao Y, Zhang M, Han X, Shang H Tags: Jpn J Infect Dis Source Type: research

Analysis of clinical isolates of extended-spectrum β-lactamase-producing bacteria with developed primer and probe sets to detect blaCTX-M, blaTEM, and blaSHV using a fully automated gene detection system.
In this study, we evaluated extended-spectrum β-lactamase (ESBL)-producing bacteria with the newly developed primer and probe sets to detect blaCTX-M, blaTEM, and blaSHV using BD MAXTM, a fully automated multiplex polymerase chain reaction assay system. In 36 isolates confirmed to have any one of blaCTX-M, blaTEM, or blaSHV by whole-genome sequencing, the product accurately detected each gene without influenced by the presence of other β-lactamase genes. In nine control strains which do not harbor either blaCTX-M, blaTEM, or blaSHV did not cross react with the product. In 191 strains phenotypically determined ESB...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Abe Y, Nakamura K, Kaji D, Takahashi H, Aoki K, Kuse H, Okada H, Ohta K, Ohashi K, Takano Y, Ishii Y, Kanemitsu K Tags: Jpn J Infect Dis Source Type: research

Viral and atypical bacterial respiratory infections in a university teaching hospital.
In this study, we aimed to detect mutiple viral and bacterial agents in the respiratory samples of inpatients by real-time PCR (RT-PCR). Nasopharyngeal swab and broncho alveolar lavage samples' results from the inpatients with respiratory infection symptoms at the Uludag University Hospital between 01.12.2015 and 31.03.2018 were investigated. DNA/RNA was extracted with EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) in EZ1 (Qiagen, Belgium). R-GENE® Real-Time PCR (Biomerioux, France) kit was used to detect Influenza A, Influenza B, RSV, human Meta-pneumo virus (hMPV), Rhino/Enterovirus, Adenovirus (AdV), human Bocavirus (hBo...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Agca H, Ener B Tags: Jpn J Infect Dis Source Type: research

Laboratory evaluation of rapid antigen detection tests for more-sensitive detection of Respiratory syncytial virus antigen.
Abstract We evaluated two currently available rapid antigen detection tests (RADTs) for Respiratory syncytial virus (RSV), Sofia® RSV FIA and BinaxNOW RSV Card (BinaxNOW). Between November 2017 and February 2018, 395 nasopharyngeal swabs were collected from children diagnosed with acute respiratory infections, and were evaluated with these RADTs, reverse transcription-quantitative real-time PCR (RT-qPCR), and a direct immunofluorescence assay (DFA). The sensitivity of Sofia® RSV FIA (80.82%) was significantly higher than that of BinaxNOW (53.42%) when RT-qPCR was used as the standard. This was confirmed wi...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Sun Y, Deng J, Qian Y, Zhu R, Wang F, Tian R, De R, Zhao L Tags: Jpn J Infect Dis Source Type: research

Dengue Virus in Traveler Returning to Japan from the Democratic Republic of the Congo, 2015.
Abstract Dengue fever (DF) is a mosquito-borne disease and significant global public health problem. Although limited serologic surveys in the literature suggest endemic DF in many parts of Africa, DF cases in Africa are generally underreported due to the lack of diagnostic testing and systematic surveillance, and thus, little is known about the phylogenetic profile of circulating strains. In April 2015, DF was diagnosed in a Japanese national returned from the Democratic Republic of the Congo (DRC). Dengue virus 1 (DENV-1) RNA was detected from the patient's serum sample by real-time reverse transcription PCR. Ph...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Yamamoto SP, Kasamatsu Y, Kanbayashi D, Kaida A, Shirano M, Kubo H, Goto T, Iritani N Tags: Jpn J Infect Dis Source Type: research

Erythromycin-susceptible but clindamycin-resistant phenotype of ermB-PCR-positive clinical group B streptococci having IS1216E-inserted ermB.
Abstract Streptococcus agalactiae (Group B Streptococcus, GBS) is a pathogen causing neonatal sepsis, meningitis, and invasive infections in the elderly and people with medical conditions. Macrolide and lincosamide resistance rates of GBS are increasing worldwide. A macrolide resistance gene, erythromycin ribosomal methylase (erm), typically confers MLSB phenotype. However, in this study, we recovered and characterized three clinical ermB-PCR-positive isolates of GBS with L phenotype. PCR of ermB and lnuB (lincosamide nucleotidyltransferase) genes were positive in all three clinical isolates. The ermB gene of the ...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Moroi H, Kimura K, Ido A, Banno H, Jin W, Wachino JI, Yamada K, Kikkawa F, Park YJ, Arakawa Y Tags: Jpn J Infect Dis Source Type: research

Extremely high biting densities of Aedes albopictus (Skuse) (Diptera: Culicidae) at a university campus in Nagasaki, Japan.
This study describes extremely high biting densities of A. albopictus at a campus of Nagasaki University, southwestern Japan. In August of 2015 and 2016, human-bait-sweep collection for 8 min obtained on average 33.4 and 38.9 females, respectively. In both 2015 and 2016, the highest and the second highest biting densities were observed in sites densely shaded by trees and covered with a lot of understory plants. In addition, major breeding sites were identified near these sites in 2016. A predaceous larval mosquito, Lutzia vorax Edwards, appeared to strongly suppress the breeding of A. albopictus in catch basins near the s...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Sunahara T Tags: Jpn J Infect Dis Source Type: research

Molecular detection of Anaplasma phagocytophilum from larvae of Haemophysalis longicornis in Ibaraki, Japan.
Abstract DNA from 1084 ticks collected by flagging in vegetation in Tsukuba and Moriya (Ibaraki, Japan), where several cases of canine granulocytic anaplasmosis were found, was molecularly examined for infection with the family Anaplasmataceae. Twenty-six positive samples of Anaplasmataceae-specific PCR of partial 16S rRNA gene were subjected to semi-nested PCR covering the divergent regions the gene and sequence analysis. Anaplasma phagocytophilum was detected in three pools of Haemophysalis longicornis larvae, and Anaplasma bovis from a Haemophysalis flava male. Sequences of both amplicons had highest homologies...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Fukui Y, Inokuma H Tags: Jpn J Infect Dis Source Type: research

Distribution and expression of IL-17 and related cytokines in children with Mycoplasma pneumoniae pneumonia.
In this study, we used FCM (Flow Cytometry) to analyze IL-17 and related cytokines in plasma and bronchoalveolar lavage fluid (BALF) samples from 18 patients with general MPP (GMPP) and 30 patients with refractory MPP (RMPP). The levels of IL-1Ra, IL-6, IL-8, IL-17 and TNF-α were significantly elevated in the BALF of MPP children compared with the plasma (P
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Fan H, Lu B, Yang D, Zhang D, Shi T, Lu G Tags: Jpn J Infect Dis Source Type: research

Human leukocyte antigen-associated HIV-1 CRF02_AG gag and vif polymorphisms in Ghana.
Abstract In human immunodeficiency virus type-1 (HIV-1) infections, cytotoxic T-lymphocyte (CTL) responses targeting human leukocyte antigen (HLA)-restricted viral epitopes exert strong suppressive pressure on viral replication and frequently select for mutations resulting in viral escape from CTL recognition. Numerous data on these HLA-associated mutations in HIV-1 subtypes B and C have been amassed with few reports described in other subtypes. In the present study, we investigated HLA-associated mutations in HIV-1 subtype CRF02_AG prevailing in Ghana, Western Africa. We determined viral gag sequences in 246 out ...
Source: Japanese Journal of Infectious Diseases - June 28, 2019 Category: Infectious Diseases Authors: Adusei-Poku MA, Matsuoka S, Bonney EY, Abana CZ, Duker EO, Nii-Trebi NI, Ofori SB, Mizutani T, Ishizaka A, Shiino T, Kawana-Tachikawa A, Ishikawa K, Ampofo WK, Matano T Tags: Jpn J Infect Dis Source Type: research

Genotype distribution of Panton-Valentine leukocidin (PVL) positive Staphylococcus aureus strains isolated from wound related infections: A three year multi-center study in Tehran, Iran.
Abstract The spread of PVL-carrying S. aureus strains in patients with wound infections in both the community and hospitals are increasing in some areas of Iran. In the present study, we determined the molecular characteristics and distribution of PVL-producing S. aureus strains isolated in wound infections. Genes encoding resistance, toxin, and staphylococcal enterotoxins were analyzed by polymerase chain reaction assays. Genotyping was performed by using multi-locus sequence typing. Aminoglycoside resistance genes including ant (4΄)-Ia gene (57.4%) and aac (6΄)-Ie/aph (2˝) (45.7%) were the most prevalent gene...
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Goudarzi M, Fazeli M, Pouriran R, Eslami G Tags: Jpn J Infect Dis Source Type: research

Isolation of OXA-181 carbapenemase-producing Escherichia coli and OXA-232 carbapenemase-producing Klebsiella pneumoniae from a pregnant woman returning from India to Japan.
PMID: 31155599 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Tamura S, Hayakawa K, Miyoshi-Akiyama T, Nakanishi M, Yano T, Shimoya K, Sugiki Y, Mezaki K, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Safety and Immunogenicity of the Quadrivalent HPV Vaccine in Japanese Boys: a Phase 3, Open-Label Study.
Abstract Human papillomavirus (HPV)-associated disease is common among men with HPV infection. A quadrivalent HPV (qHPV) vaccine has demonstrated 85.9% efficacy against HPV6/11/16/18-related persistent (≥6 month) infection in a study of Japanese men aged 16-26 yr. Here, we report results of an open-label study of the immunogenicity and tolerability of the qHPV vaccine (NCT02576054), conducted to bridge findings in Japanese men to Japanese boys aged 9-15 yr. A total of 100 boys completed a three-vaccination regimen (Day 1, Months 2, and 6), and 99 boys were included in the primary analysis population. The rate o...
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Murata S, Takeuchi Y, Yamanaka K, Hayakawa J, Yoshida M, Yokokawa R, Wakana A, Sawata M, Tanaka Y Tags: Jpn J Infect Dis Source Type: research

Acute liver failure associated with influenza A virus infection: an autopsy case report.
Abstract An 84-year-old man with chronic renal failure, anemia, and diabetes was admitted for hemodialysis initiation. His vital signs were stable until the eighteenth hospital day, before having an influenza A virus infection. Three days later, he died of septic shock with severe liver impairment. His leukocyte count, prothrombin time (PT-INR), and liver enzymes, such as AST and ALT, were significantly increased. Hypercytokinemia was also observed. Autopsy revealed bilateral diffuse pneumonia with neutrophil infiltration. The liver showed extensive centrilobular hepatocyte necrosis. Immunohistochemistry for influ...
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Nonaka K, Matsuda Y, Kakizaki M, Takakuma S, Hamamatsu A, Sakashita Y, Matsubara T, Murayama S, Ishiwata T, Yamanaka N, Itabashi M, Takei T, Nakajima N, Hasegawa H, Arai T Tags: Jpn J Infect Dis Source Type: research

A novel genotype of Leptospira interrogans recovered from leptospirosis outbreak in southern Thailand.
Abstract   We performed Leptospira culture of 76 clinical samples collected from animals and 6 soil samples for the investigation of a leptospirosis outbreak in southern Thailand, 2017. Recovery of leptospires was found in a kidney sample of a fatal canine leptospirosis case and all of the soil samples. 16S rRNA sequence analysis demonstrated that the clinical isolate was closely related to the pathogenic L. interrogans while the soil isolates were related to different species including the pathogenic L. ellisii, the intermediate L. wolffii and the non-pathogenic L. yanagawae. Multilocus sequence typing ...
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Thongdee M, Chaiwattanarungruengpaisan S, Lekcharoen P, Yimchoho N, Buathong R, Wiriyarat W Tags: Jpn J Infect Dis Source Type: research

Predominant detection of the subgroup A2b human metapneumovirus strain with 111-nucleotide duplication in Yokohama City, Japan in 2018.
Abstract   Human metapneumovirus (HMPV) has been a major causative agent of acute respiratory infections in humans. Recently two types of variant A2b subtype HMPV strains possessing 111- or 180-nucleotide duplication (nt-dup) in the G gene (HMPV A2b180nt-dup and HMPVA2b111nt-dup, respectively) were detected in Japan, Spain, Vietnam, and China. Surveillance for infectious agents in Yokohama City, Japan revealed that the HMPV111nt-dup strain became predominant in Yokohama City in 2018. In contrast, no classical HMPV A2b strain was detected after 2017. These data suggest a beneficial role of the 111nt-dup i...
Source: Japanese Journal of Infectious Diseases - May 31, 2019 Category: Infectious Diseases Authors: Saikusa M, Nao N, Kawakami C, Usuku S, Tanaka N, Tahara M, Takeda M, Okubo I Tags: Jpn J Infect Dis Source Type: research

Histoplasmosis among HIV-infected patients in Japan: A case report and literature review.
We report a case of disseminated histoplasmosis in a human immunodeficiency virus (HIV)-infected Japanese man who had often travelled to histoplasmosis-endemic countries. We also reviewed the reported cases of HIV-associated histoplasmosis in Japan. To the best of our knowledge, this is the ninth case report of co-infection with Histoplasma and HIV in Japan, and the second involving a Japanese patient. This case emphasizes the importance of noting the details of not only the present residence of patients but also their previous residence and travels. If histoplasmosis is suspected, physicians should inform laboratory perso...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Hatakeyama S, Okamoto K, Ogura K, Sugita C, Nagi M Tags: Jpn J Infect Dis Source Type: research

Regional variation of antimicrobial use in Japan from 2013-2016, estimated by sales data.
Abstract The National Action Plan on Antimicrobial Resistance in Japan aims to achieve a 50% reduction in the use of broad-spectrum oral-antimicrobials (cephalosporins, macrolides, and quinolones) from 2013 to 2020. We estimated regional antimicrobial use (AMU) during 2013-2016 using national antimicrobial sales data, and evaluated differences in broad-spectrum oral-antimicrobials AMU among three regions with previous differences in total AMU. AMU was standardized by defined daily dose (DDD) and described as DDDs/1,000 inhabitants/day (DID). Annual combined total-oral and total-parenteral AMU during 2013-2016 was ...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Kusama Y, Ishikane M, Tanaka C, Kimura Y, Yumura E, Hayakawa K, Muraki Y, Yamasaki D, Tanabe M, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Attack rate-patient number plot for analysis of food poisonings caused by microbes and plant or animal toxins.
Abstract The number of eaters and that of patients per outbreak followed lognormal distribution in food poisonings caused by microbes while it followed scale-free distribution in food poisoning caused by plant or animal toxins. Attack rates of the individual outbreaks were distributed continuously and almost linearly from>0 to 1 for all the food poisonings, i.e., they could not be represented by median and standard deviation. For monitoring of the number of patients and the attack rate in individual outbreaks simultaneously, the number of patients was plotted in the x-axis in the logarithmic scale against the a...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

An epidemic of hand, foot, and mouth disease caused by coxsackievirus A6 in Osaka City, Japan, in 2017.
Abstract The second largest epidemic of hand, foot, and mouth disease since 1982 occurred in 2017, which involved 6,173 cases in Osaka City, Japan. The main causative agent was coxsackievirus A6 (CV-A6). Phylogenetic analysis revealed that the detected CV-A6 strains belonged to genetic group A3 and A4 in clade A. PMID: 31061358 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Kanbayashi D, Kaida A, Hirai Y, Yamamoto SP, Fujimori R, Okada M, Kubo H, Iritani N Tags: Jpn J Infect Dis Source Type: research

Dengue virus type 2 infection in a traveler returning from Saudi Arabia to Japan.
Abstract In July 2018, a Japanese traveler returning from Saudi Arabia was diagnosed with dengue. Dengue virus type 2 gene was detected from the whole blood sample. Phylogenetic analysis revealed that the strain clustered with isolates from Singapore and India. Travelers to Saudi Arabia should be cautious about mosquito bites. PMID: 31061359 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Matsui T, Kinoshita N, Maeki T, Kutsuna S, Nakamura K, Nakamoto T, Ishikane M, Tajima S, Kato F, Taniguchi S, Lim CK, Saijo M, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Serotypes, pathogenic potential and antimicrobial resistance of Escherichia coli isolated from subclinical bovine mastitis milk samples in Egypt.
In this study, E. coli were isolated from fourteen (9.3%) SCM milk samples. The isolated E. coli (n=14) were serotyped, their potential pathogenicity and antimicrobial resistances (AMRs) were investigated. Serotyping results showed that the E. coli isolates belonged to the O55:H7 (n=2), O111:H4 (n=2), O127:H6 (n=2), O128:HUN (n=2), O26:HUN (n=1), O44:H18 (n=1), O114:H21 (n=1), O86:HUN (n=1), O124:HUN (n=1) and O127:H7 (n=1) serogroups. Potential pathogenicity was detected in 93% (13/14) of the isolates. In particular, thirteen isolates possessed at least one of the examined virulence genes. Ten isolates (71%) exhibited AMR...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Ombarak RA, Zayda MG, Hinenoya A, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Shift to older ages of HIV/AIDS deaths and its gender difference: inference derived from Vital Statistics of Japan.
Abstract Death of HIV/AIDS patients 55 years old started increasing. Though deaths directly linked to infections declined since 2005, those related to malignancy, encephalopathy, interstitial pneumonia, wasting syndrome, etc. persisted. For all of HIV/AIDS (mainly males), ATL, CJD and general population, age of death shifted to older ages by 5 years in 10 years from 1999-2004 to 2010-2017. Among them, only HIV/AIDS patients and general population exhibited unequivocal gender difference. As of 2011-2016, the median of the deaths of the HIV/AIDS patients was 52.5 years for males and 70 years for females, while the m...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Rapid identification and quantification of Lactobacillus rhamnosus-targeting real-time PCR using a TaqMan probe.
In this study, we developed an L. rhamnosus-targeting quantitative PCR assay, which produces accurate and reproducible results based on the specificity of a TaqMan probe targeting the unique 16S rDNA sequence of L. rhamnosus. The assay specifically detected the targeted bacterium, L. rhamnosus, and no non-specific signals were generated under the study conditions. Using genomic DNA from the bacterial cells of L. rhamnosus (101 to 106 cells), the cycle threshold value showed a linear trend (R2 = 0.9993). This L. rhamnosus-targeting quantitative PCR assay can contribute to advance research into the effects of the organism on...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Okai C, Itani Y, Furuta A, Mizunoe Y, Iwase T Tags: Jpn J Infect Dis Source Type: research

First report of TEM-116 and OXA-10 extended-spectrum β-lactamase in clinical isolates of Alcaligenes species from Kuala Lumpur, Malaysia.
First report of TEM-116 and OXA-10 extended-spectrum β-lactamase in clinical isolates of Alcaligenes species from Kuala Lumpur, Malaysia. Jpn J Infect Dis. 2019 Mar 26;: Authors: Puah SM, Puthucheary SD, Chua KH Abstract There is an alarming increase of extended-spectrum β-lactamases (ESBL) being present mainly in Enterobacteriaceae and other non-fermenting gram-negative bacteria, such as Alcaligenes faecalis which is the only species present in that genus that is clinically relevant. We investigated Alcaligenes species from 7 cases (6 inpatients and 1 outpatient) at our tertiary hospital. F...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Puah SM, Puthucheary SD, Chua KH Tags: Jpn J Infect Dis Source Type: research

Poor sleep quality is associated with higher risk of pulmonary tuberculosis among patients with type 2 diabetes mellitus course more than 5 years.
In conclusion, poor sleep quality is an independent risk factor of PTB among DM patients with course>5 years, which indicates significant epidemiological implications for PTB control. PMID: 30918145 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Kou T, Wang Q, Lv W, Wei B, Liu Y, Zhao S, Du X, Zou Y, Sun L, Tian H, Zhao Z, Liu Q, Dong C, Zhang G, Gao X, Yu C Tags: Jpn J Infect Dis Source Type: research

Enhancing preparedness against rubella at the workplace: proactive prevention efforts by the Tokyo Metropolitan Government.
Abstract During Japan's 2012-2013 rubella epidemic, most transmission in Tokyo occurred at workplaces, especially among men aged 20 to 49. This tendency was likely related to previous rubella immunization policies that prioritized female adolescents. Since 2015, the Tokyo Metropolitan Government has been promoting a project to prevent rubella and other infectious diseases in workplaces. Companies participate by choosing one or more of three options: (I) Acquire fundamental understanding of infectious diseases; (II) Develop a Business Continuity Plan (BCP) for infectious diseases in the workplace; or (III) Increase...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Sugishita Y, Soejima K, Kayebeta A, Yauchi M Tags: Jpn J Infect Dis Source Type: research

Epidemiologic Trends and Clinical Features of Pneumocystis jirovecii Pneumonia in Non-HIV Patients in a Tertiary-Care Hospital in Korea over a 15-Year-Period.
Abstract Subsequent to the increasing use of immunosuppressant therapy, Pneumocystis jirovecii pneumonia (PcP) has emerged as a life-threatening condition in human immunodeficiency virus (HIV)-negative patients. We investigated changes in epidemiological and clinical characteristics among PcP cases with and without HIV infections. Data of 424 patients diagnosed with PcP in a 2,700-bed Korean tertiary care hospital between February 2003 and April 2017 were retrospectively analyzed. The study included patients with compatible clinical findings in whom PcP was confirmed via direct immunofluorescence assay. The annual...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Lee HY, Choi SH, Kim T, Chang J, Kim SH, Lee SO, Kim MN, Sung H Tags: Jpn J Infect Dis Source Type: research

Clinical evaluation of a newly developed chemiluminescent enzyme immunoassay in Japan for hepatitis C virus core antigen.
In conclusion, LP-Presto shows rapid, reliable performance. Nonspecific testing results due to hyperimmunoglobulinemia were reduced by LP-Presto. LP-Presto is a high-quality HCVcAg assay that shows promise for various applications. PMID: 30918148 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Inoue T, Ohike T, Ohne K, Sato S, Goto T, Tanaka Y Tags: Jpn J Infect Dis Source Type: research

Predominance of ST22-MRSA-IV clone and Emergence of Clones for Methicillin-resistant Staphylococcus aureus clinical isolates collected from a Tertiary Teaching Hospital over a two-year period.
This study aimed to determine the genotypic and phenotypic characteristics of clinical MRSA isolates collected from a teaching hospital from 2014 - 2015. These isolates were genotyped by multilocus sequence typing (MLST), staphylococcal cassette chromosomal mec (SCCmec) typing, virulence genes detection and pulsed-field gel electrophoresis (PFGE) and phenotyped based on their vancomycin susceptibility profiles. The most prevalent sequence type was ST22. ST3547 was identified from a blood isolate from 2015. Three SCCmec types (III in 26.26%, IV in 70.71% and V in 3.03%) were detected. Agr type I, II, III were detected among...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Niek WK, Teh CSJ, Idris N, Thong KL, Ponnampalavanar S Tags: Jpn J Infect Dis Source Type: research