A healthcare-associated outbreak of urinary tract infections due to Myroides odoratimimus.
We report a healthcare-associated outbreak of urinary tract infections caused by Myroides odoratimimus in a Turkish hospital. From March to May 2019, six strains of M. odoratimimus were isolated from the urine samples of patients hospitalized in the intensive care units (ICUs). After identification and antibiotic susceptibility testing with VITEK 2 system, MALDI-TOF-MS and 16S rRNA based sequencing methods were performed for confirmation and species level identification. Pulsed-field gel electrophoresis (PFGE) was used to investigate clonal relatedness of the isolates. All the patients were immunocompromised and underwent ...
Source: Japanese Journal of Infectious Diseases - November 19, 2020 Category: Infectious Diseases Authors: Kutlu HH, Avcı M, Dal T, Arı O, Durmaz R Tags: Jpn J Infect Dis Source Type: research

Evaluation the anti-Mycobacterial activity of newly synthesized (S)-N-(3-(2-fluoro-4'-(2-amino-4-thiazolyl)biphenyl-4-yl)-2-oxo-1,3-oxazolidie-5-ylmethyl) acetamide derivative in vitro and in Mycobacterium marinum infected zebrafish.
Abstract Our previous work have identified a novel oxazolidinones structure, biaryloxazolidinone analogues containing a hydrazone moiety, as promising antibacterial agents against Gram positive strains. Based on these active structure, we synthesized a series novel oxazolidinones here and determine their anti-Mycobacterial activities in vitro and in Mycobacterium marinum (M.marinum) infected Zebra fish. The in vitro anti-Mycobacterial assay demonstrated that all of them have potent efficacy against both H37Rv and clinical Mycobacterial isolates. Among of these active agents, (S)-N-(3-(2-fluoro-4'-(2-amino-4-thiazo...
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Ding X, Wu Y, Tong A, Sun B, Zhao Y, Jiang Y Tags: Jpn J Infect Dis Source Type: research

Immunogenicity and Safety of a Two-Dose Series of a Meningococcal (Groups A, C, W, and Y) Polysaccharide Diphtheria Toxoid Conjugate Vaccine (MEN ACWY-D) in Healthy Japanese Adults.
This study (NCT02591290) evaluated the safety and immunogenicity of two doses administered eight weeks apart to 60 healthy Japanese adults aged 20-55 years. Blood samples were collected 28-35 days after each vaccination. Immunogenicity endpoints included seroprotection and seroconversion rates. Safety assessments included systemic adverse events (AEs), non-serious AEs, and serious AEs. Fifty-eight (96.7%) participants completed the study. Seroprotection rates for serogroups A, C, W, and Y before vaccination were 76.8%, 26.8%, 26.8%, and 50.0%, increasing to 100%, 83.9%, 91.1%, and 96.4% and 100%, 92.9%, 94.6%, and 94.6%, r...
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Matsuoka O, Tsuzuki D, Suzuki K, Tanaka T, Takagi Y, Oster P Tags: Jpn J Infect Dis Source Type: research

Acute ischemic and hemorrhagic stroke in two covid-19 patients.
We report two cases with coincident presentation of COVID-19 and cerebrovascular accident. Further studies are needed for a comprehensive understanding of the neurological pathology of COVID-19 and its effects on the nervous system, but stroke teams should be wary of the fact that COVID-19 patients can present with cerebrovascular accidents. PMID: 33132298 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Urciuoli L, Guerriero E, Musto L Tags: Jpn J Infect Dis Source Type: research

Availability of chlamydia trachomatis testing and its importance in surveillance of genital chlamydial infection.
This study surveyed the prevalence of GCTI in 456 hospitals in Shandong province, China in December 2018. Among the hospitals surveyed, antigen testing, PCR testing, and either antigen or PCR testing were available in 200 (43.9%), 88 (19.3%) and 268 (58.8%) hospitals, respectively. PCR testing was more available in tertiary hospitals than in primary and secondary hospitals (X2=28.560, P=0.000). Significant differences were observed in the availability of antigen testing (X2=15.708, P=0.003), PCR testing (X2=22.494, P=0.000), and either antigen or PCR testing (X2=21.729, P=0.000) among different types of hospital. In 2018, ...
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Chen X, Gan Y, Chang D, Mi X, Liu D Tags: Jpn J Infect Dis Source Type: research

Does quinolone- or macrolide-resistant Streptococcus agalactiae bacteremia affect patient outcome? A multicenter cohort study.
Abstract The prevalence of quinolone- and macrolide-resistant Group B Streptococcus (GBS) is increasing worldwide, but the relationship between GBS resistance to these antibiotics and patient outcome remains unclear. Therefore, we evaluated whether blood stream infection caused by quinolone- or macrolide-resistant GBS is associated with high mortality. Our findings in 77 patients with GBS bacteremia demonstrate that quinolone and macrolide resistance may not be risk factors for 30-day mortality. PMID: 33132300 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Mitsuboshi S, Date J, Tsuruma N, Yamaga H, Watanabe K, Kijima H, Nakashita M, Hosokawa H, Tsugita M Tags: Jpn J Infect Dis Source Type: research

Epidemiological and phylogenetic analysis of mumps virus isolated from 2016 to 2019 in Henan Province, China.
Abstract Routine vaccination has proven to be highly effective in reducing the incidence of mumps. However, sporadic cases and/or mumps outbreaks do occur in children and adolescents younger than 15 years old, particularly among those 5-9 years old. To explore the characteristics of such outbreaks in Henan Province, clinical data of patients infected with MuV were collected, and the isolated MuV strains were phylogenetically analyzed. Of a total of 426 samples, MuV RNA targeting the small hydrophobic (SH) gene was detected in 153 samples. MuV-positive cases in each age group (
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Ma J, Wang P, Tang J, Zheng L, Li S, Huo Y Tags: Jpn J Infect Dis Source Type: research

Development and validation of a new prognostic score system for COVID-19.
This study aimed to develop and validate a bedside risk analysis system for predicting the clinical severity and prognosis of patients with COVID-19. A total of 444 COVID-19 patients were included and were randomly assigned into two groups at a ratio of 2:1 as derivation and validation groups. The new scoring system comprised of eight variables, which included history of having malignant diseases, history of having diabetes mellitus, dyspnea, respiratory rate of>24 bpm, C-reactive protein (CRP) of>14 mg/L, white blood cell count of>8×109/L, platelets count of
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Ma K, Xia Y, Hu B, Zhang Y, Xu X, Zhang N, Xu H Tags: Jpn J Infect Dis Source Type: research

Rapid and simple detection of isoniazid resistant Mycobacterium tuberculosis utilizing DNA chromatography based technique.
This study aimed to develop a simple, rapid and low-cost diagnostic kit for isoniazid resistant tuberculosis using single-stranded tag hybridization method targeting an isoniazid resistance conferring mutation. Specificity and sensitivity were assessed by utilizing DNA extracted from 49 isoniazid resistant and 41 susceptible Mycobacterium tuberculosis clinical isolates cultured in Mycobacterial Growth Indicator Tubes. Positive signals were observed at mutant and wildtype lines with the sensitivity and specificity of 100% comparing to sanger sequencing results. In contrast, no positive signal was observed with non-tuberculo...
Source: Japanese Journal of Infectious Diseases - October 30, 2020 Category: Infectious Diseases Authors: Kodera T, Yamaguchi T, Fukushima Y, Kobayashi K, Takarada Y, Chizimu JY, Nakajima C, Solo ES, Lungu PS, Kawase M, Suzuki Y Tags: Jpn J Infect Dis Source Type: research

Isolation and identification of pathogenic Acanthamoeba species from air conditioning systems, Egypt.
This study aimed to identify Acanthamoeba isolated from air conditioning systems by culture method and Polymerase Chain Reaction (PCR) and testing its pathogenicity by thermo-tolerance and animal inoculation. Out of the 80 dust samples, 41 (51.25%) were found to be positive as Acanthamoeba spp. by culture method. These samples were confirmed positive by PCR. Regarding pathogenicity, out of 41 samples, 27 (65.9%) were thermo-tolerant and 16 (39%) samples could infect mice and caused histopathological effects. Highly pathogenic Acanthamoeba isolates were characterized by a thermo-tolerance ability and disseminated in all mic...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Gabr NS, Mohamed RM, Belal US, Abdel-Fatah MM, Ahmed RF, Gaber NATA, Abdel-Hafeez EH Tags: Jpn J Infect Dis Source Type: research

Interim 2019/2020 influenza vaccine effectiveness in Japan from October 2019 to January 2020.
Abstract Herein we report the interim vaccine effectiveness (VE) of quadrivalent inactivated influenza vaccine for the 2019/2020 influenza season in Japan. We conducted a retrospective observational cohort study of 381 enrolled influenza-like illness patients aged ≥ 15 years who were examined via the rapid-influenza diagnostic test at the Ambulatory Care unit of the National Center for Global Health and Medicine in Tokyo, Japan from the beginning of October 2019 to the end of January 2020. VE was estimated using a test-negative design. VE was calculated as (1 - odds ratio) × 100%, comparing influenza ...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Tsuzuki S, Ishikane M, Matsunaga N, Morioka S, Yu J, Inagaki T, Yamamoto M, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Daptomycin Susceptibility of Group B Streptococcus.
This study aimed to analyze the daptomycin susceptibility among the 1,046 clinical GBS isolates that were recovered since the approval of daptomycin in Japan. MICs of daptomycin for the 1,046 clinical isolates were determined by the microdilution method recommended by the CLSI. The MIC range was 0.12-1 µg/ml, and the MIC50 and MIC90 were 0.5 µg/ml and 1 µg/ml, respectively. None of the GBS isolates evaluated in this study was non-susceptible to daptomycin. Therefore, at present, daptomycin might be considered as a new option to treat GBS infections, especially, multidrug-resistant PRGBS infections. P...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Ushino T, Jin W, Wachino JI, Arakawa Y, Kimura K Tags: Jpn J Infect Dis Source Type: research

Severe Apparent Life-threatening Event (ALTE) in an Infant with SARS-CoV 2 Infection.
Abstract The 2019 novel coronavirus (severe acute respiratory syndrome-coronavirus: SARS-CoV-2) has currently caused a global outbreak of infection. In general, children with the coronavirus disease-2019 have been reported to show milder respiratory symptoms as a respiratory infection than adult patients. Here, we describe SARS-CoV-2 infection in an infant who presented with a severe episode of apparent life-threatening event (ALTE). An 8-month-old otherwise healthy infant who was transported to our hospital because of a sudden cardiopulmonary arrest. Approximately one hour before this episode, she was almost fine...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Sano F, Yagasaki H, Kojika S, Toda T, Kono Y, Suzuki-Inoue K, Sasaki T, Ogihara S, Matsuno T, Inoue O, Moriguchi T, Harii N, Goto J, Shimizu T, Inukai T Tags: Jpn J Infect Dis Source Type: research

Dissemination of Polymyxin B Sensitivity in El Tor Vibrio cholerae O1 Strains in Odisha, India.
Abstract The Vibrio species undergo cryptic changes in their genetic material for better adaptability, which accounts for antibiotic resistance. In the present study, we investigated the emergence and spread of sensitivity to polymyxin B by V. cholerae O1 El Tor strains from 1995 to 2019 in Odisha, India. The results exhibited that out of 1200 V. cholerae O1 strains, 89.4% were resistant and remaining 10.6% strains were sensitive to polymyxin B. The sensitivity to polymyxin B by V. cholerae O1 strains emerged from 2005 to 2019 except 2015 that clearly signifies the presence of classical biotype characteristics pos...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Pal BB, Behera DR, Nayak SR, Nayak AK, Biswal B, Pati S Tags: Jpn J Infect Dis Source Type: research

Burden of pediatric central nervous system infection and cost-benefit simulation of multiplex PCR in Japan.
Abstract To investigate the clinical use of multiplex polymerase chain reaction (mPCR) in Japan, epidemiological and clinical data for central nervous infections are needed. Here we report on the epidemiology and economic burden of central nervous system infections and a simulation of cost-benefit analysis of the Filmarray® Meningitis/Encephalitis (FAME) test for possible clinical use in Japan. We performed FAME test on samples from 27 patients with pleocytosis aged between 0 and 20 years seen in six community hospitals in Nara and Osaka prefectures. All clinical management had been performed without knowledge...
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Kitano T, Nishikawa H, Suzuki R, Onaka M, Nishiyama A, Kitagawa D, Oka M, Masuo K, Yoshida S Tags: Jpn J Infect Dis Source Type: research

Isolation of coxsackievirus A21 from patients with acute respiratory infection in Yamagata, Japan in 2019.
PMID: 32999187 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 29, 2020 Category: Infectious Diseases Authors: Ikeda T, Aoki Y, Komabayashi K, Itagaki T, Mizuta K Tags: Jpn J Infect Dis Source Type: research

The clinical trend of leprosy from 2000-2016 in Kaohsiung, a major international harbor city in Taiwan, where leprosy is almost extinguished.
This study aims to characterize the clinical and histopathological features of patients with leprosy in the context of near elimination. 15 cases of pathologically proven leprosy were identified from 2000-2016 in Kaohsiung Chang Gung Memorial Hospital. The clinical presentations, demographic details, treatment responses, and disease outcome were reviewed. The mean age was 46 years (range: 26 to 73 years). Eight cases were native Taiwanese, while six and one cases were foreign workers from Indonesia and, Thailand, respectively. The diagnosis was made 3-6 months in average after skin lesions occurred. The most common clinica...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Huang HW, Lee CH, Lin SH Tags: Jpn J Infect Dis Source Type: research

Virulence profile of diarrhoeagenic Escherichia coli from the Western region of Ghana.
This study provides insights into the important and novel genotypes circulating in the Western region of Ghana that should be monitored for public health. PMID: 32863350 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Prah I, Ayibieke A, Huong NTT, Iguchi A, Mahazu S, Sato W, Hayashi T, Yamaoka S, Suzuki T, Iwanaga S, Ablordey A, Saito R Tags: Jpn J Infect Dis Source Type: research

Spread of Haitian variant Vibrio cholerae O1 causing cholera outbreaks in Odisha, India.
This study envisages the characterisation of isolated pathogen from two cholera outbreaks reported in 2018 and 2019 from Bargarh and Rayagada districts of Odisha respectively. Vibrio cholerae O1 were isolated following standard techniques. The different virulent and drug resistant genes were detected by multiplex PCR assays; whereas the ctxB genotypes were characterised through double mismatch amplification mutation (DMAMA) PCR assay. The ctxB genes were further sequenced and pulse-field gel electrophoresis (PFGE) was done on some selected strains. The clinical and water isolates of Haitian variant (HCT) V.cholerae O1 Ogaw...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Nayak SR, Nayak AK, Biswal BL, Pati S, Pal BB Tags: Jpn J Infect Dis Source Type: research

The Intracellular Invasion Ability and the Associated Microbiological Characteristics of Streptococcus canis in Isolates from Japan.
This study evaluated cell invasion ability (CIA) of Streptococcus canis isolates and clarified the relationship between high-frequency CIA and microbiological features. Of the companion animal-origin isolates (n = 117) in 2017, 40 isolates were randomly selected with the host information, and two human blood-origin isolates were included. We measured the CIA using human colon carcinoma epithelium and the hemolytic activity (HA) using sheep blood, along with S. canis M-like protein (SCM) allele typing, sequence type (ST) determination, and antimicrobial resistance (AMR) phenotyping/genotyping. CIA measurements revealed that...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Yoshida H, Goto M, Fukushima Y, Maeda T, Tsuyuki Y, Takahashi T Tags: Jpn J Infect Dis Source Type: research

Growth characteristics of Rickettsia species LON strains closely related to Rickettsia japonica isolated from Haemaphysalis longicornis ticks in mouse-derived L929 and human-derived THP-1 host cell lines.
In this study, microscopic analysis showed that LONs in a mouse-derived L929 host cell line are rod shaped with sizes of 0.3-0.5 x 0.5- 2.0 μm. Molecular-based analysis revealed the existence of LON-specific disrupted open reading frame in R. japonica-related group specific DNA region. Growth kinetics of LON-2 and LON-13 strains analyzed by a quantitative real-time PCR showed 100-fold increment or more of LONs cultured in L929 host cells at 30oC and slightly less increment at 33oC, and 25-fold increment in human-derived THP-1 host cells at 35oC on day 7 (168 h) post infection. The generation times of 2 LON strains cultu...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Tai H, Su H, Takamoto N, Fujita H, Takano A, Oishi S, Abe F, Ando S, Ohashi N Tags: Jpn J Infect Dis Source Type: research

Detection of tick-borne pathogens in ticks from dogs and cats in Yamagata Prefecture, Japan, 2018.
Abstract Companion animals can become infected with tick-borne diseases (TBDs) and can become hosts of transmission to humans, thereby damaging human health. To clarify whether companion animals are infested by ticks harboring TBD pathogens in humans, we detected TBD pathogens in ticks collected from dogs and cats brought to animal hospitals in Yamagata Prefecture, Japan. Investigation of 164 adult ticks collected from 88 dogs and 41 cats during March-July 2018 revealed the predominant tick species as Ixodes ovatus (n = 95, 57.9%), followed by Ixodes nipponensis (37, 22.6%) and Haemaphysalis flava (10, 6.1%). The ...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Seto J, Tanaka S, Kawabata H, Ito Y, Ikeda T, Mizuta K Tags: Jpn J Infect Dis Source Type: research

Geographic correlation between the number of COVID-19 cases and the number of overseas travelers in Japan, Jan-Feb, 2020.
Abstract Since the coronavirus disease 2019 (COVID-19) emerged in Wuhan, China, in December 2019, it has rapidly spread around the world, the number of patients is also increasing in Japan. The number of COVID-19 cases in Japan in the early stage was not uniform and were largely concentrated in several prefectures. The distribution of the COVID-19 cases and the number of foreign travelers as well as Chinese travelers at prefectural level showed a strong positive correlation coefficient of 0.68 (P
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Wagatsuma K, Phyu WW, Osada H, W Tang J, Saito R Tags: Jpn J Infect Dis Source Type: research

Antiviral effect of favipiravir (T-705) against measles and subacute sclerosing panencephalitis viruses.
In this study, the antiviral effect of favipiravir against measles virus (Edmonston strain) and SSPE virus (Yamagata-1 strain) was examined in vitro. The 50% effective concentrations of favipiravir (inhibiting viral plaque formation by 50%) for Edmonston and Yamagata-1 strains were 108.7 ± 2.0 μM (17.1 ± 0.3 μg/mL) and 38.6 ± 6.0 μM (6.1 ± 0.9 μg/mL), respectively, which were similar to those of ribavirin. The antiviral activity of favipiravir against the SSPE virus was demonstrated, for the first time, in this study. PMID: 32863356 [PubMed - as supplied by publisher] (Source: J...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Hashimoto K, Maeda H, Miyazaki K, Watanabe M, Norito S, Maeda R, Kume Y, Ono T, Chishiki M, Suyama K, Sato M, Hosoya M Tags: Jpn J Infect Dis Source Type: research

current status, international collaboration, and future directions of a comprehensive antimicrobial resistance surveillance system.
Abstract The Japanese Nosocomial Infections Surveillance (JANIS) is one of the largest national antimicrobial resistance (AMR) surveillance systems in the world. In particular, the JANIS Clinical Laboratory (CL) division collects comprehensive specimen-based data from diagnostic microbiology laboratories of participating hospitals to monitor the isolation rate of 11 major bacteria and specific AMR bacteria, and creating antibiograms of approximately 20 bacterial species. Data in the JANIS web-database system are also annually tabulated and provided to the WHO Global Antimicrobial Resistance Surveillance System (GL...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Kajihara T, Yahara K, Hirabayashi A, Shibayama K, Sugai M Tags: Jpn J Infect Dis Source Type: research

Splenic abscess owing to Salmonella Typhi: An uncommon presentation.
This report reiterates the importance of clinical diagnosis and radiological imaging even for the common diseases when presents in an unusual fashion. Percutaneous drainage and microbial culture with susceptibility based antibiotics are the key to its treatment. PMID: 32863358 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Kaur N, Varshney VK, Kombade SP, Yadav T, Suman S, Garg PK, Nag VL Tags: Jpn J Infect Dis Source Type: research

When should clinicians repeat SARS-CoV-2 RT-PCR?: Repeat PCR testing targeting patients with pulmonary CT findings suggestive of COVID-19.
Abstract Real-time reverse transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 is sometimes repeated when clinicians suspect a false-negative result, but the conditions under which repeated RT-PCR testing is warranted remain unclear. We evaluated the practice of repeat RT-PCR testing for SARS-CoV-2 in 45 patients who retested after an initial negative PCR test. Of these, the diagnosis of coronavirus disease (COVID-19) was confirmed in four patients with typical chest computed tomography (CT) findings, and one patient without typical CT findings in whom the test result was strongly suspected to ...
Source: Japanese Journal of Infectious Diseases - August 30, 2020 Category: Infectious Diseases Authors: Yamamoto K, Saito S, Hayakawa K, Hashimoto M, Takasaki J, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Dengue Virus Serotype 1 Exported to Japan from Cote d'Ivoire, 2019.
Abstract Dengue fever outbreaks have been repeatedly reported in Cote d'Ivoire. In 2019 outbreak, DENV-1 was predominant and phylogenetic analysis of the DENV-1 strain detected in a present patient who returning to Japan in January 2019 revealed high homology with the 2013-2014 Southeast Asian strains. Previous outbreak in 2017, DENV-1 accounted for 5% of the DENV serotypes. The endemic DENV-1 strain in Abidjan in 2019 could be a strain that was imported from Southeast Asia. Dengue virus spreads intercontinentally and imported dengue fever case could be an alert for the outbreaks in the exporting country. PMI...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Suzuki T, Kutsuna S, Nakamoto T, Ota M, Ishikane M, Yamamoto K, Maeki T, Tajima S, Nakayama E, Taniguchi S, Lim CK, Saijo M, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Pulmonary nocardiosis with superior vena cava syndrome in HIV-infected patient: A rare case report in the world.
Abstract Pulmonary nocardiosis is a common disease among HIV-infected patients. In most cases, the disease progresses slowly. Here, we present a case whose disease progressed rapidly. A 35-year-old female with AIDS and superior vena cava (SVC) syndrome who was lost to follow-up visited our hospital. She presented with a chronic non-productive cough and her CD4 count was 33 (4%). Her chest x-ray showed opacity in the right upper lobe of her lung and her sputum acid-fast stain was negative. Anti-tuberculosis agents were prescribed. Two weeks later, superficial vein dilatation appeared on her chest wall and her chest...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Kiatsuranon P, Suwanpimolkul G Tags: Jpn J Infect Dis Source Type: research

Isolation of Serratia fonticola producing FONA, a minor extended-spectrum β-lactamase (ESBL), from imported chicken meat in Japan.
Isolation of Serratia fonticola producing FONA, a minor extended-spectrum β-lactamase (ESBL), from imported chicken meat in Japan. Jpn J Infect Dis. 2020 Aug 01;: Authors: Tanimoto K, Nomura T, Hashimoto Y, Hirakawa H, Watanabe H, Tomita H Abstract Five novel strains of Serratia fonticola that produce FONA, a minor extended spectrum beta-lactamase (ESBL), were isolated during the routine surveillance of ESBLproducing Enterobacteriaceae in imported chicken meat in Japan in 2017 and 2018. These strains exhibited a clear ESBL phenotype in susceptibility tests carried out in the presence of clavulani...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Tanimoto K, Nomura T, Hashimoto Y, Hirakawa H, Watanabe H, Tomita H Tags: Jpn J Infect Dis Source Type: research

Preterm twins from the mother with miliary tuberculosis: Importance of early recognition and prompt response in infection control to manage congenital tuberculosis exposure in a neonatal intensive care unit.
Abstract Delayed diagnosis of congenital tuberculosis (TB) in a neonatal intensive care unit (NICU) is a serious problem in terms of infection control. Here, we report our preemptive infection control activities implemented after the diagnosis of miliary TB in a mother of preterm twins (index twins, NB1 and NB2) in NICU. Added to this, we reviewed previous case reports of congenital TB exposure in the NICU setting. Immediately after recognizing miliary TB of the mother, the index twins were isolated before their TB diagnosis and received preemptive antiTB medication, and contact investigations were also conducted....
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Ryu BH, Baek EH, Kim DH, Kim SE, Kim HJ, Cho OH, Hong SI, Do HJ, Park CH Tags: Jpn J Infect Dis Source Type: research

Positive stool test suggests that the discharge standard of COVID-19 needs to be improved.
We report a hospitalized patient with COVID-19 whose fecal samples turned negative 22 days later than the respiratory samples. It highlights that the duration of virus release from patients is longer than previously expected. Current clinical examinations for treatment and discharge standard are limited to respiratory samples. However, we believe that nucleic acid testing of both respiratory and fecal samples is necessary for discharged patients. Further studies are needed to confirm the potential for fecal-oral transmission or fecal-respiratory transmission via aerosols. PMID: 32741930 [PubMed - as supplied by publis...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Ge R, Chen Z, Liu X, Zhang Q, Zhu G, Xiao Q Tags: Jpn J Infect Dis Source Type: research

Predictors of coronavirus disease 2019 severity: A retrospective study of 64 cases.
Abstract To analyze clinical characteristics and potential predictors of disease severity in patients with COVID-19.Clinical data from 64 patients with COVID-19 were retrospectively analyzed. Of the 64 patients, 37 were male and 27 were female. Their mean age was 47.8 years, 43 (67.2%) cases were non-severe, 21 (32.8%) were severe, and 2 patients (3.1%) died. Age and serum ferritin were significantly associated with COVID-19 severity. Repeated monitoring of ferritin, interleukin-6, C-reactive protein, lactic acid dehydrogenase, and erythrocyte sedimentation rate during COVID-19 treatment may assist the prediction ...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Huang H, Song B, Xu Z, Jiao Y, Huang L, Zhao P, Huang J, Zhou Z, Zhao Z, Tian J, Zhou Y, Wang FS, Jiang T Tags: Jpn J Infect Dis Source Type: research

Characterziation of JC polyomavirus derived from COS-IMRb cells.
In this study, we carried out long term cultivation of COS-7 cells (designated as COS-IMRb cells) transfected with PML type M1-IMRb, an adapted viral DNA with a rearranged non-coding control region (NCCR). The JCPyV derived from COS-IMRb cells was characterized by analyzing viral replication, amount of virus by hemagglutination (HA), production of viral protein 1 (VP1), and structure of the NCCR. HA assays indicated that a high amount of PML type JCPyV was present in COS-IMRb cells. Immunostaining showed that VP1-positive cells were a small population in COS-IMRb cells that represented a JCPyV carrier cell culture. Sequenc...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Nukuzuma S, Nukuzuma C, Kameoka M, Sugiura S, Nakamichi K, Tasaki T, Hidaka K, Takegami T Tags: Jpn J Infect Dis Source Type: research

Threat of dissemination, Panton-Valentine leukocidin-positive livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) CC398 clone in Tokyo, Japan.
Abstract In the last decade, methicillin-resistant Staphylococcus aureus (MRSA) has been identified in livestock animals, such as swine, poultry, and veal calves, and termed livestock-associated MRSA (LA-MRSA). LA-MRSA sequence type (ST) 398 strains can effectively colonize and infect humans, with subsequent transmission in both community and hospital settings. Unlike other countries, LA-MRSA was not yet reported in Japanese patients until 2019. However, we recently reported a case of intractable arthritis caused by a LA-MRSA CC398 (ST1232) clone, which is a single locus variant of ST398, in a patient in Tokyo, Ja...
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Nakaminami H, Kawasaki H, Takadama S, Kaneko H, Suzuki Y, Maruyama H, Noguchi N Tags: Jpn J Infect Dis Source Type: research

Seasonality of human coronavirus OC43, NL63, HKU1, and 229E infection in Yamagata, Japan, 2010-2019.
PMID: 32741934 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 31, 2020 Category: Infectious Diseases Authors: Komabayashi K, Seto J, Matoba Y, Aoki Y, Tanaka S, Ikeda T, Matsuzaki Y, Itagaki T, Mizuta K Tags: Jpn J Infect Dis Source Type: research

Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan.
Abstract During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan. PMID: 32074516 [PubMed - indexed for MEDLINE] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 21, 2020 Category: Infectious Diseases Authors: Shirato K, Nao N, Katano H, Takayama I, Saito S, Kato F, Katoh H, Sakata M, Nakatsu Y, Mori Y, Kageyama T, Matsuyama S, Takeda M Tags: Jpn J Infect Dis Source Type: research

Characteristics of SARS-CoV-2 Isolated from Asymptomatic Carriers in Tokyo.
PMID: 32350227 [PubMed - indexed for MEDLINE] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - July 21, 2020 Category: Infectious Diseases Authors: Nagashima M, Kumagai R, Yoshida I, Kawakami M, Nagano M, Asakura H, Kaku E, Kitamura Y, Hasegawa M, Hayashi Y, Chiba T, Sadamasu K, Yoshimura K Tags: Jpn J Infect Dis Source Type: research

Prevalence of high-level aminoglycoside resistance and genes encoding aminoglycoside-modifying enzymes in Enterococcus faecalis and Enterococcus faecium isolated in a university hospital in Tokyo.
We examined the prevalence of HLAR by disk diffusion method, carriage of genes for aminoglycoside-modifying enzymes by PCR, and production of β-lactamase by nitrocefin-based test in Enterococcus faecalis and Enterococcus faecium isolated from patients at a university hospital in Tokyo in 2010. Of the 100 E. faecalis isolates analyzed, 30 isolates had high-level resistance (HLR) to gentamicin and 22 isolates had HLR to streptomycin. Of the 40 E. faecium isolates analyzed, nine isolates had HLR to gentamicin and nine isolates had HLR to streptomycin. Of the 39 gentamicin-HLR enterococcal isolates, 24 isolates were non-H...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Harada S, Shibue Y, Aoki K, Ishii Y, Tateda K Tags: Jpn J Infect Dis Source Type: research

An Intracranial Tuberculoma With Discharging Scalp Sinus.
PMID: 32611970 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Li L, Cheng C, Shetty R, Li S, Wu T, Zhao N Tags: Jpn J Infect Dis Source Type: research

Circulation of Various Dengue Serotypes in Community Based Study in Jakarta, Indonesia.
In this study, we developed active DENV cases finding in the community in Jakarta to study circulating dengue serotypes where adult febrile patients with fever less than 48 hours were recruited. Disease severities were defined using the World Health Organization (WHO) 1997 guideline. Rapid NS1 dengue antigen detection were used to screen DENV patients in the community. Viral isolation in C6/36 cell line or increased antibody titer by HI and ELISA or RT-PCR were performed to confirm DENV infection.Viral serotype was investigated through RT-PCR. From 102 patients, 68 (66.7%) patients were confirmed DENV infection with DENV-2...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Dewi BE, Nainggolan L, Sudiro TM, Chenderawasi S, Goentoro PL, Sjatha F Tags: Jpn J Infect Dis Source Type: research

Serum 25-hydroxy vitamin D levels in Japanese infants younger than 3-months old with respiratory syncytial virus infection.
Abstract Low levels of blood vitamin D have been reported in children who have frequent respiratory tract infections. We measured serum concentrations of 25-hydroxy (OH) vitamin D in Japanese infants less than 3-months old infected with respiratory syncytial virus (RSV). Serum levels of 25-OH vitamin D of the 10 infants, excluding those with underlying diseases, were between less than 4 to 29.8 ng/mL. In 8 out of 10 subjects (80.0%), serum 25-OH vitamin D levels were less than 20 ng/mL. There was no statistically significant association between levels of 25-OH vitamin D and age, duration of admission, respiratory ...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Kawashima H, Kimura M, Morichi S, Nishimata S, Yamanaka G, Kashiwagi Y Tags: Jpn J Infect Dis Source Type: research

Changing demography of genital chlamydia, gonorrhea, genital herpes, condyloma and syphilis infections in Japan.
Abstract There was epidemiological correlation in time and in space between gonorrhea and genital chlamydia and between condyloma and genital herpes. There was, however, no correlation in other combinations, such as, gonorrhea and condyloma, genital herpes and chlamydia, etc. The annual incidence of gonorrhea and that of genital chlamydia were mono-phasic with a peak in 2005-2006. The annual incidence of condyloma and that of genital herpes were biphasic with the first distinct peak in 2004-2006 borne by younger generation and the second broad peak borne by the older generation that experienced the first wave.The ...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Spatiotemporal variability of Zoonotic Cutaneous Leishmaniasis based on sociodemographic heterogeneity. The case of Northeastern Iran, 2011-2016.
Abstract Zoonotic Cutaneous Leishmaniasis (ZCL) is one of the most prevalent zoonoses in Iran, especially in its central and northeast parts. This research aims to examine if there are spatiotemporal clusters of the ZCL cases, and if so, whether there are disparities in clustering according to age, gender, home situation, and occupation. The spatial analysis, including global and local spatial autocorrelations, inverse distance weighting, and space-time scan statistics were applied to determine potential clusters in Golestan villages during 2011-2016. Several spatially significant (p
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Tabasi M, Alesheikh AA Tags: Jpn J Infect Dis Source Type: research

Analysis of HPV genotypes and liquid-based cervical cytology: Results from a tertiary academic center in northwestern Turkey.
uml;r Ş, Önal B Abstract High-risk human papilloma virus (hrHPV) plays an important role in the cause of cervical cancer. The aim of this study is to investigate the distribution of HPV genotypes in the region and to correlate with the liquid-based-cytology (LBC) and colposcopic biopsy results. Furthermore, the potential relationship between HPV infections and bacterial vaginosis (BV) was investigated. HPV genotypes were determined by real-time PCR using a commercial kit at molecular microbiology. LBC, biopsies and BV examinations were performed at Pathology& Cytology. The consecutive cervical specimens ...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: ÇaliŞkan E, CoŞkun SK, ÖztÜrk CE, CangÜr Ş, Önal B Tags: Jpn J Infect Dis Source Type: research

Multiple-locus variable-number tandem repeat analysis for non-O157 Shiga toxin-producing Escherichia coli : focus on serogroups O103, O121, O145, O165, and O91.
In this study, we designed an MLVA scheme (MLVA43) by adding 26 loci to an MLVA scheme (MLVA17) previously developed by our group for O157, O26, and O111 using 17 loci; the current scheme focused on serogroups O103, O121, O145, O165, and O91. Discriminatory power of MLVA43 was comparable to that of pulsed-field gel electrophoresis (PFGE) for serogroups O103, O145, O165, and O91, and superior to that of PFGE for O121. MLVA43 identified more profiles than did MLVA17, except for serogroup O111 with 707 isolates. The new MLVA43 scheme would enable rapid identification of clusters and outbreaks, which will help rapid measure ag...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Izumiya H, Lee K, Ishijima N, Iyoda S, Ohnishi M Tags: Jpn J Infect Dis Source Type: research

The epidemiology of admission-requiring pediatric respiratory infections in a Japanese community hospital using multiplex PCR.
This study aimed to assess the epidemiology of admission-requiring pediatric RTI cases and to evaluate the effect of pathogen type on length of hospital stay (LOS) using the FilmArray® respiratory panel, a multiplex PCR test. The age-specific distribution and seasonality of viruses were investigated between March 26, 2018 and April 12, 2019. Multivariable linear regression analyses were performed to evaluate the effect of pathogen type and coinfection on LOS. Among 153 hospitalized RTI patients, respiratory syncytial virus (RSV) was the leading cause of hospitalization in infants
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Inagaki A, Kitano T, Nishikawa H, Suzuki R, Onaka M, Nishiyama A, Kitagawa D, Oka M, Masuo K, Yoshida S Tags: Jpn J Infect Dis Source Type: research

SARS-CoV-2 in Italy: Population Density correlates with Morbidity and Mortality.
Abstract Since the beginning of the SARS-CoV-2/COVID-19 epidemic in China, elderly and multimorbid subjects showed a higher mortality rate. However, other factors could influence the mortality and the spread of contagion such as the population density. An archival research based on the Italian data stratified by region was performed in order to quantify the association between the population density, ageing index, number of positive cases, number of deaths, case-fatality rate, and medical equipment (gloves, masks, and ventilators). Results showed a significant positive linear relationship between the population de...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Ilardi A, Chieffi S, Iavarone A, Ilardi CR Tags: Jpn J Infect Dis Source Type: research

Risk factors analysis and nomogram construction of non-survivors in critical patients with COVID-19.
Abstract A pandemic named coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbroke in China since December 2019. This disease has caused more than 70000 deaths worldwide. We intend to analyze the risk factors of death and establish a prognosis nomogram for critical patients with COVID-19. We analyzed the clinical data of COVID-19 patients in Zhongnan Hospital of Wuhan University who were critical cases with COVID-19 before March 20, 2020. Data were collected at admission and compared between survivors and non-survivors and analyzed by univariable and multi...
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Wang B, Zhong F, Zhang H, An W, Liao M, Cao Y Tags: Jpn J Infect Dis Source Type: research

Chest CT evaluation of 11 persistent asymptomatic patients with SARS-CoV-2 infection.
In conclusion, typical CT findings can be detected in over 70% of asymptomatic SARS-CoV-2 carriers. It mainly starts as GGO along subpleural regions and bronchi, and absorbs in nearly 5 days. PMID: 32611980 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - June 29, 2020 Category: Infectious Diseases Authors: Yan S, Chen H, Xie RM, Guan CS, Xue M, Lv ZB, Wei LG, Bai Y, Chen BD Tags: Jpn J Infect Dis Source Type: research