The dominant antigenic group of influenza C infections changed from c/sao paulo/378/82-lineage to c/kanagawa/1/76-lineage in yamagata, Japan, in 2014.
PMID: 25797148 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 25, 2015 Category: Infectious Diseases Authors: Tanaka S, Aoki Y, Matoba Y, Yahagi K, Mizuta K, Itagaki T, Katsushima F, Katsushima Y, Matsuzaki Y Tags: Jpn J Infect Dis Source Type: research

A National Point-Prevalence Survey of Pediatric Intensive Care Unit-Acquired, Healthcare-Associated Infections in Turkey.
The objective of this point prevalence study was to assess the burden of HCAIs in the PICUs. The study conducted in 50 PICUs across Turkey. Data on demographics, microbiological findings, and therapeutic interventions and outcomes were collected for all PICU inpatients. A total of 327 patients participated in the study, 122 of whom had one or more HCAI, corresponding to a prevalence of 37%. The most frequently reported site was lower respiratory tract (n=77, 63%). The most frequent pathogens were Pseudomonas aeruginosa, Acinetobacter spp. and Candida spp. Two hundred and forty-seven patients (75%) were receiving antimicrob...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Kepenekli E, Soysal A, Yalindag-Ozturk N, Ozgur O, Ozcan I, Devrim I, Akar S, Bakir M, Turkish PICU-HCAI Study Group Tags: Jpn J Infect Dis Source Type: research

Detection of Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples using ELISA as a Laboratory Diagnostic Method for Dengue Virus Infection.
Abstract Dengue virus (DENV) infection is a serious global health threat. For dengue disease surveillance and control, there is a need for robust diagnostic tools, which are relatively easy to use and reliable in various clinical settings. We determined the utility of NS1 antigen detection in urine samples for DENV diagnosis using 118 urine samples obtained from 96 dengue patients at various phases of disease. NS1 antigen was detected by ELISA in urine samples of dengue patients obtained at days 2-17 after disease onset. Positive detection rates of NS1 antigen ranged from 13-43%. Using real-time RT-PCR, positive d...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Saito Y, Moi ML, Kotaki A, Ikeda M, Tajima S, Shiba H, Hosono K, Saijo M, Kurane I, Takasaki T Tags: Jpn J Infect Dis Source Type: research

Immunogenicity of a candidate DNA vaccine based on the prM/E genes of a dengue type 2 virus Cosmopolitan genotype.
Abstract The development of a dengue vaccine is a major priority. Several researchers are using Asian 1 and Asian 2 genotypes as vaccine candidates to develop a vaccine for dengue type 2 virus (DENV-2). However, in this study, we constructed a recombinant plasmid-based prM-E gene from the DENV-2 Cosmopolitan genotype as a dengue DNA vaccine candidate. The protein expression levels of recombinant plasmids in CHO cell lines were analyzed using an enzyme-linked immunosorbent assay, western blotting, and sucrose gradients. After immunizing ddY mice three times, DNA vaccine doses of 25 and 100 µg induced humoral ...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Putri DH, Sudiro TM, Yunita R, Jaya UA, Dewi BE, Sjatha F, Konishi E, Hotta H, Sudarmono P Tags: Jpn J Infect Dis Source Type: research

Sexual practices and the prevalence of HIV and syphilis among men who have sex with men in Lanzhou, China.
Abstract To assess the risk of sexual practices and explore the determinants of the prevalence of HIV and syphilis among men who have sex with men (MSM) in Lanzhou, China, a survey was conducted among 600 MSM from March 2008 to December 2009, demographic information and sexually behavioral factors were collected with snowball sampling technology and blood samples were obtained. Multivariate logistic regression models were used to determine the predictors of HIV and syphilis infection. The sera-prevalence of HIV, syphilis, HCV and co-infection of HIV with syphilis was 6.0%, 18.0%, 1.3% and 2.5% respectively. Incons...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Gao W, Li Z, Li Y, Qiao X Tags: Jpn J Infect Dis Source Type: research

Pseudomonas oleovorans sepsis in a child: the first reported case in India.
PMID: 25766604 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Gautam L, Kaur R, Kumar S, Bansal A, Gautam V, Singh M, Ray P Tags: Jpn J Infect Dis Source Type: research

Genomic analysis for single nucleotide polymorphisms (Asp299Gly and Thr399lle) in Japanese patients with invasive aspergillosis.
Abstract Single nucleotide polymorphisms (SNPs) in the gene encoding Toll-like receptor 4 (TLR4), 1063A/G (Asp299Gly) and 1363C/T (Thr399Ile), have been reported to be associated with increased susceptibility to invasive aspergillosis. However, little is known regarding the prevalence of such SNPs in Japan. We therefore surveyed these SNPs in the TLR4 gene using formalin-fixed and paraffin-embedded (FFPE) tissue blocks from autopsies of patients diagnosed with invasive pulmonary aspergillosis. Finally, approximately 30% of cases were available for genomic analysis and none of them showed the SNPs: 1063A/G (Asp299G...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Aki K, Okubo Y, Nanjo H, Ishiwatari T, Nihonyanagi Y, Tochigi N, Wakayama M, Nemoto T, Fukutake K, Shinozaki M, Hori Y, Masuda H, Shibuya K Tags: Jpn J Infect Dis Source Type: research

Outbreak of cholera by multidrug resistant Vibrio cholerae O1 in a back ward taluka of Bagalkot, North Karnataka.
Abstract Cholera is a major cause of illness in the developing world. During the monsoon, sporadic and small clusters of cases of cholera are encountered almost every year in Karnataka, India. During the monsoons in 2013, there was emergence of cholera in Badami, a remote backward area of Bagalkot district in Karnataka, by multi drug resistant V. cholerae O1 serotype Ogawa. The outbreak of diarrhoea started on 5(th) August, 2013, when a 30 year old woman reported with severe dehydration and watery diarrhoea at the Aganwadi Health Centre in Somanakoppa village of Badami. A total of 49 cases suspected of cholera rep...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Bhattacharya D, Dey S, Roy S, Parande MV, Telsang M, Seema MH, Parande AV, Mantur BG Tags: Jpn J Infect Dis Source Type: research

Active Surveillance of Methicillin-Resistant Staphylococcus aureus with the Fully Automated Molecular Test in an Emergency Medical Center.
Abstract The prevention and control of methicillin-resistant Staphylococcus aureus (MRSA) is an important practice, particularly in emergency units. The active surveillance of MRSA was prospectively performed at the emergency medical center (EMC) in Nagasaki University Hospital. After obtaining nasal swab specimens, a fully automated molecular test (FAMT) and a culture-screening method were performed for MRSA detection. A total of 150 patients enrolled in the study and 366 nasal swab specimens were obtained. MRSA was detected by the culture in 11 (7.3%) patients including one new acquisition, and by the FAMT in 34...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Morinaga Y, Yamano S, Akamatsu N, Kaku N, Nagaoka K, Migiyama Y, Harada Y, Hosogaya N, Yamamoto Y, Tasaki O, Yanagihara K, Kohno S Tags: Jpn J Infect Dis Source Type: research

Coexistence of Avian Influenza Virus H10 and H9 Subtypes among Chickens in Live Poultry Markets during an Outbreak of Human Infection with Novel H10N8 Virus in Nanchang, China.
We report the results of a cross sectional study on avian influenza virus (AIV) in live poultry markets (LPMs) in Nanchang, China after the first patient case with H10N8 virus was reported in the city. A total of 201 specimens tested positive for AIVs of the 618 samples collected from 24 LPMs in Nanchang from December 2013 to January 2014. We found that the LPMs were heavily contaminated by AIVs, with H9, H10 and H5 being the predominant subtypes and more than half of the LPMs providing samples positive for H10 subtype. Moreover, the coexistence of different subtypes was common in LPMs. Of the 201 positive samples, 20.9% (...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Hu M, Li X, Ni X, Wu J, Gao R, Xia W, Wang D, He F, Chen S, Liu Y, Guo S, Li H, Shu Y, Bethel JW, Liu M, Moore JB, Chen H Tags: Jpn J Infect Dis Source Type: research

Severe toxoplasmic hepatitis in an immunocompetent patient.
We present an immunocompetent patient with toxoplasmic hepatitis of serious complicated clinical course in whom maculopapular rash was present. Diagnosis was confirmed by serology, and presence of bradyzoites in liver biopsy. PMID: 25766609 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Atilla A, Aydin S, Demirdöven AN, Kiliç SS Tags: Jpn J Infect Dis Source Type: research

Turtle-associated Salmonella infections in Kanagawa, Japan.
We present case reports of salmonellosis with reptile-related serotypes of Salmonella enterica subspecies enterica. The first case was a 5-year-old boy with gastroenteritis caused by Salmonella enterica subspecies enterica serovar Poona. A turtle, which was suspected as the source of infection, was kept at the patient's home. The second case was a 4-year-old boy with gastroenteritis caused by S. enterica subspecies enterica serovar Abony. The PFGE analysis suggested that a tortoise, which was kept at the patient's home, was the source of infection. We review the literature on turtle-associated salmonellosis in Japan in tan...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Kuroki T, Ito K, Ishihara T, Furukawa I, Kaneko A, Suzuki Y, Seto J, Kamiyama T Tags: Jpn J Infect Dis Source Type: research

Susceptibility of Indigenous and Transplanted Mosquito spp. in Japan to Dengue Virus.
Abstract Dengue fever is an acute, mosquito-borne, febrile illness caused by Flavivirus spp., which is a problem in Africa, South and Southeast Asia, Latin America, and the Caribbean. A dengue outbreak occurred after nearly 70 years of absence or no detection, and 158 autochthous cases occurred in Japan from August to October 15, 2014. The most competent mosquito vectors for dengue virus transmission were Aedes aegypti and A. albopictus. Since A. albopictus is widely distributed throughout Japan and A. aegypti recently invaded Japan by airplane, we examined the susceptibility of these species to infection by dengu...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Sasaki T, Higa Y, Bertuso AG, Isawa H, Takasaki T, Minakawa N, Sawabe K Tags: Jpn J Infect Dis Source Type: research

Association between RABV G Proteins Transported from the Perinuclear Space to Cell Surface Membrane and N-glycosylation of the Sequon at Asn(204).
Abstract Viral G proteins of the rabies virus (RABV) Kyoto strain were detected in the cytoplasm but not distributed at the cell membrane of mouse neuroblastoma (MNA) cells. Viral G proteins of CVS-26 were detected in both the cell membrane and perinuclear space of MNA cells. We found that N-glycosylation of street RABV G protein by the insertion of the sequon at Asn(204) induced the transfer of RABV G proteins to the cell surface membrane. Fixed RABV bud from the plasma membrane depend not only on G protein but also on other structural proteins such as M protein. However, different N-glycosylation of G protein co...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Hamamoto N, Uda A, Tobiume M, Park CH, Noguchi A, Kaku Y, Okutani A, Morikawa S, Inoue S Tags: Jpn J Infect Dis Source Type: research

Biting density and distribution of Aedes albopictus during the September 2014 outbreak of dengue fever in Yoyogi Park and the vicinity in Tokyo Metropolis, Japan.
This study aimed to collect referential data to estimate the transmission threshold of dengue virus in terms of biting density of Ae. albopictus and demonstrated high transmission-risk areas of dengue virus in Yoyogi Park and the vicinity. The overall mean density of biting Ae. albopictus, 7.13/man/8 min, was sufficiently high for successful transmission of dengue virus, and areas with biting densities higher than the overall mean density were classified as high transmission-risk areas of dengue virus in Yoyogi Park. PMID: 25766613 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Tsuda Y, Maekawa Y, Ogawa K, Itokawa K, Komagata O, Sasaki T, Isawa H, Tomita T, Sawabe K Tags: Jpn J Infect Dis Source Type: research

Genetic Variations of Human Papillomavirus Type 16: Implications for Cervical Carcinogenesis.
Abstract Human papillomaviruses (HPVs) are the causative agent of cervical cancer, and among approximately 15 high-risk genotypes, HPV16 accounts for more than half the cases of cervical cancer worldwide. Recent progress in determining HPV genomic sequences from clinical samples has revealed a wide variety of HPV16 genome sequences, and has allowed the comprehensive classification of intratype HPV16 variants. These consist of four variant lineages containing nucleotide variations in 1.0-10.0% of the complete viral genome sequences. Epidemiological data suggest that the non-European-Asian lineages of HPV16 entail a...
Source: Japanese Journal of Infectious Diseases - March 13, 2015 Category: Infectious Diseases Authors: Kukimoto I, Muramatsu M Tags: Jpn J Infect Dis Source Type: research

Epidemiological Investigation of a Norovirus GII.4 Sydney Outbreak in a China Elder Care Facility.
Abstract An outbreak of norovirus GII.4/Sydney_2012 affected a China elder care facility in December 2012. A total of 39 elderly people and staff met the outbreak case definition. The attack rates in the elderly and the staff were 15.9% (31/195) and 23.2% (19/82), respectively, including 13 asymptomatic cases in the staff. The result of gene sequencing revealed that the outbreak was caused by norovirus GII.4 Sydney. The mode of transmission of this outbreak was proven to be person-to-person. The first case (a self-cared elder) was affected outside the elder care facility and was not isolated after returning. Norov...
Source: Japanese Journal of Infectious Diseases - February 26, 2015 Category: Infectious Diseases Authors: Zheng QM, Zeng HT, Dai CW, Zhang SX, Zhang Z, Mei SJ, He YQ, Ma HW Tags: Jpn J Infect Dis Source Type: research

Emergence of Salmonella Strain That Produces IMP-1-Type Metallo-β-Lactamase in a Japanese Patient.
Emergence of Salmonella Strain That Produces IMP-1-Type Metallo-β-Lactamase in a Japanese Patient. Jpn J Infect Dis. 2015;68(1):75-6 Authors: Hosoda T, Wakuda M, Ishii J, Tsuge I, Matsui M, Suzuki S, Yamada K, Suzuki K, Ishikawa K, Yoshida S PMID: 25702791 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 26, 2015 Category: Infectious Diseases Authors: Hosoda T, Wakuda M, Ishii J, Tsuge I, Matsui M, Suzuki S, Yamada K, Suzuki K, Ishikawa K, Yoshida S Tags: Jpn J Infect Dis Source Type: research

Molecular Typing of Mycoplasma pneumoniae Isolated from Pediatric Patients in Tokyo, Japan.
PMID: 25702792 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 26, 2015 Category: Infectious Diseases Authors: Kubota H, Okuno R, Hatakeyama K, Uchitani Y, Sadamasu K, Hirai A, Kai A Tags: Jpn J Infect Dis Source Type: research

Picornavirus-Like Cytopathic Effects on RD-18S Cell Lines Were Induced by Human Coronavirus 229E Not Picornaviruses.
PMID: 25702793 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 26, 2015 Category: Infectious Diseases Authors: Matoba Y, Aoki Y, Tanaka S, Yahagi K, Itagaki T, Matsuzaki Y, Mizuta K Tags: Jpn J Infect Dis Source Type: research

Impact of population size on incidence of rubella and measles in comparison with that of other infectious diseases.
Abstract Volume 67, no. 6, p.447-457, 2014. Page 448, Figure 1 should appear as shown below.Page 449, ``log4 or 0.6 log10'' in the 9th line in the left column should read ``1.6 log10,'' and (``http://www.japan-now.com/article/343318758.html [in Japanese]'') in the 10-11th lines should read (``http://ecitizen.jp/Ssds/Indicators/_A01202 [in Japanese]).'' PMID: 25702794 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - February 26, 2015 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Caffeic acid, a coffee-related organic acid, inhibits the propagation of hepatitis C virus.
Abstract Based on multipurpose cohort studies, coffee consumption reduces the risk of hepatocellular carcinoma, one of the main causes of which is hepatitis C virus (HCV) infection. Here, we focused on the effect of caffeic acid, a major organic acid derived from coffee, on the propagation of HCV using an in vitro naïve HCV particle-infection and production system within human hepatoma-derived Huh7.5.1-8 cells. When cells were treated with 1% coffee extract or 0.1% caffeic acid for 1-h after HCV infection, the amount of HCV particles released into the medium at 3 and 4 days post-infection were considerably de...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Tanida I, Shirasago Y, Suzuki R, Abe R, Wakita T, Hanada K, Fukasawa M Tags: Jpn J Infect Dis Source Type: research

Biochemical features and virulence gene profiles of non-O157/O26 enterohemorrhagic Escherichia coli strains from humans in Yamaguchi Prefecture, Japan.
Abstract The biochemical features and virulence gene profiles of 37 enterohemorrhagic Escherichia coli (EHEC) strains belonging to serogroups other than O157 and O26 (non-O157/O26 EHEC) were investigated. All strains were isolated from humans between 2002 and 2013 in Yamaguchi Prefecture. Serogroup O111 strains were most common, followed by O103, O121, and O145. Most strains (84%) were negative for sorbose fermentation, whereas only one and two were negative for sorbitol and rhamnose fermentation, respectively. Two strains lacked β-D-glucuronidase activity. stx subtyping revealed six genotypes:stx1a (n=20), s...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Kameyama M, Yabata J, Nomura Y, Tominaga K Tags: Jpn J Infect Dis Source Type: research

Chlorine dioxide is a superior disinfectant against multi-drug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii.
In this study, we evaluated the antibacterial activity of chlorine dioxide (ClO2) compared with sodium hypochlorite (NaClO) on various multidrug-resistant strains in the presence of bovine serum albumin and sheep erythrocytes to mimic the frequent blood contamination in clinical environment. The 3 most important species causing nosocomial infections, i.e., methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa (MDRP) and multidrug-resistant Acinetobacter baumannii (MDRA) were evaluated, with 3 representative strains from each. At a 10 ppm-concentration, ClO2 drastically reduced the n...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Hinenoya A, Awasthi SP, Yasuda N, Shima A, Morino H, Koizumi T, Fukuda T, Miura T, Shibata T, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Enhanced Surveillance for Sports Festival in Tokyo 2013: Preparation for Tokyo 2020 Olympic and Paralympic Games.
Abstract The enhanced surveillance was conducted during the Sports Festival in Tokyo 2013 (September 28-October 14, 2013), for early detection of outbreaks of infectious diseases and other health emergencies.Through this enhanced surveillance, there were 15 cases which required additional process for gathering information outside the routine process of creating/evaluating the Daily Report. However, none of the 15 cases was assessed as critical. Through the enhanced surveillance, we were able to structure a framework that allows for earlier response when detecting aberrations. It includes the role of the Tokyo Metr...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Shimatani N, Sugishita Y, Sugawara T, Nakamura Y, Ohkusa Y, Yamagishi T, Matsui T, Kawano M, Watase H, Morikawa Y, Oishi K Tags: Jpn J Infect Dis Source Type: research

Isolation and characterization of Campylobacter strains from diarrheal patients in Bangkok and its suburb in Thailand.
In this study, we attempted to isolate Campylobacter from diarrheal patients in Thailand and to characterize the species by a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for C. jejuni, C. coli and C. fetus. The species of Campylobacters were also confirmed by 16S rRNA gene sequencing and detection of hipO gene. Of 2,500 stool specimens of diarrhea 76 Campylobacter-like organisms was isolated and identified by conventional culture methods. Among 76 campylobacters 73 were identified to be 43 C. jejuni, 29 C. coli, and 1 C. fetus, respectively, by the multiplex PCR assay, however 3 were u...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Samosornsuk W, Asakura M, Yoshida E, Taguchi T, Eampokalap B, Chaicumpa W, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Clinical outcome of Linezolid in the Treatment of Extensively Drug-resistant Tuberculosis in Beijing, China: A Hospital-based retrospective Study.
Abstract linezolid, showed good clinical outcome against multidrug-resistant (MDR) and extensively drug-resistant tuberculosis(XDR-TB) in some reports, but its efficacy in indiviual TB patients and its precise contribution to combination regimens remain unclear in Chinese patients. In this paper, we described our study conducted with the aim to further characterize clinical outcome and safety of linezolid in adults with XDR pulmonary TB in Chinese PLA 309 hospital. Totally 16 XDR-TB patients received linezolid 600 mg daily and vitamin B6 50-100 mg daily as part of their individualised treatment regimens. Of the 16...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Liu Y, Bao P, Wang D, Li Y, Tang L, Zhou Y, Zhao W Tags: Jpn J Infect Dis Source Type: research

In vitro activities of 16 antimicrobial agents against Gram-negative bacteria from six teaching hospitals in China.
In conclusion, carbapenems (biapenem, meropenem, and imipenem), colistin, amikacin, and arbekacin exhibited high antimicrobial activities against Gram-negative bacteria, except for A. baumannii. PMID: 25672407 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Chen H, Wang Z, Li H, Wang Q, Zhao C, He W, Wang X, Zhang F, Wang H Tags: Jpn J Infect Dis Source Type: research

Epidemiology, antimicrobial resistance and β-lactamase genotype features of enteropathogenic Escherichia. coli isolated from children with diarrhea in Southern China.
Epidemiology, antimicrobial resistance and β-lactamase genotype features of enteropathogenic Escherichia. coli isolated from children with diarrhea in Southern China. Jpn J Infect Dis. 2015 Jan 20; Authors: Huang Y, Shan XF, Deng H, Huang YJ, Mu XP, Huang AL, Long QX Abstract The main objective of this study was to investigate epidemiology, drug resistance and β-lactamase genotype distribution of enteropathogenic Escherichia. coli (EPEC) isolated from pediatric patients with diarrhea in southern China. The prevalence rate of EPEC in children with diarrhea was 3.53%. O55:K59 and O126:K71 were...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Huang Y, Shan XF, Deng H, Huang YJ, Mu XP, Huang AL, Long QX Tags: Jpn J Infect Dis Source Type: research

Molecular Epidemiology of Human Rhinovirus infection in Mongolian 2008-2013.
Abstract Rhinovirus infections are common in all age groupsworld-wide, and they occur throughout the year. We have studied 2,689 nasopharyngeal swabs collected in 2008-2013 in Mongolia. Human rhinoviruses (HRVs) have been detected in 295(11.0%) of samples and 85(28.8%) of patients were co-infected with other respiratory viruses. Human rhinovirus (HRV) was frequently co-detected with bocavirus 21(24.7%), human coronavirus 17(20.0%) and RSV 14(16.5%) respectively.We have tested 57.6% (170/295) of all HRV positive samples of which 117 HRV strains were typed by the VP4/VP2 method and 53 additional HRV strains by 5'UTR...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Tsatsral S, Xiang Z, Fuji N, Maitsetseg C, Khulan J, Oshitani H, Wang J, Nymadawa P Tags: Jpn J Infect Dis Source Type: research

Different responses in MMPs/TIMPs expression of U937 and HepG2 cells to dengue virus infection.
Abstract Disease severities following Dengue virus (DV) infection are caused by increased vascular permeability leading to hypovolemic shock. Matrix metalloproteinases (MMPs) are believed to play a key role in promoting such severities. A previous study reported that supernatants of DV-infected DCs which contained overproduction of MMP-2 and MMP-9 induced vascular leakage in a mouse model. In the present work, we investigated whether hepatocytes (HepG2) and monocytes (U937) could be additional sources of MMPs during DV infection. HepG2 and U937 cells were exposed to DV 2 strain 16681. The secretion of MMP-2 and MM...
Source: Japanese Journal of Infectious Diseases - January 20, 2015 Category: Infectious Diseases Authors: Seanpong P, Srisaowakarn C, Thammaporn A, Leardkamolkarn V, Kumkate S Tags: Jpn J Infect Dis Source Type: research

Development of an Infectious Surrogate Hepatitis C Virus Based on a Recombinant Vesicular Stomatitis Virus Expressing Hepatitis C Virus Envelope Glycoproteins and Green Fluorescent Protein.
In this study, to develop an infectious surrogate HCV based on an rVSV (VSV/HCV), we generated a novel rVSV encoding the native E1/E2 (H77 strain) and green fluorescent protein (GFP) instead of G. We show that this VSV/HCV efficiently entered human liver cells, including Huh-7 cells, expressed GFP in them, and propagated, but did not do so in nonsusceptible BHK-21 cells. The infectivity of the VSV/HCV, which was measured as the number of foci of GFP-positive cells, was specifically reduced by the addition to the cultures of chimpanzee anti-HCV serum, anti-E2 antibody, or anti-CD81 antibody. When sera obtained from HCV-infe...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Okuma K, Fukagawa K, Tateyama S, Kohma T, Mochida K, Hiyoshi M, Takahama Y, Hamaguchi Y, Hirose K, Buonocore L, Rose JK, Mizuochi T, Hamaguchi I Tags: Jpn J Infect Dis Source Type: research

Seroprevalence of Yellow Fever virus in selected health facilities in Western Kenya 2010-2012.
Abstract Yellow fever (YF), a mosquito-borne virus, is an important viral hemorrhagic fever endemic in equatorial Africa and South America. Yellow fever virus (YFV) is the prototype of the family Flaviviridae and the genus Flavivirus. The aim of this study was to determine the seroprevalence of YFV in selected health facilities in Western Kenya during the period 2010-2012. A total of 469 serum samples from febrile patients were tested for YFV antibodies using in-house IgM-capture ELISA, in-house IgG indirect ELISA and focus reduction neutralization test (FRNT50). The present study did not yield any IgM ELISA posit...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Kwallah AO, Inoue S, Thairu-Muigai AW, Kuttoh N, Morita K, Mwau M Tags: Jpn J Infect Dis Source Type: research

Ventilator-associated Pneumonia: Epidemiology and Prognostic Factors of 30-day Mortality.
Abstract A retrospective cohort study was conducted in the medical intensive care unit of University Hospital to describe the epidemiology of Ventilator-associated pneumonia (VAP) and identify the prognostic factors of 30-day mortality. A total of 621 patients with a diagnosis of VAP between January 2005 and December 2011 were included. The overall 30-day mortality rate was 44.4%. The major causative pathogens were Acinetobacter baumannii (54.3%), followed by Pseudomonas aeruginosa (35.2%) and Methicillin-resistant Staphylococcus aureus (MRSA) (15.1%). Most A.baumannii (90.2%) comprised drug-resistant strains. The...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Inchai J, Pothirat C, Liwsrisakun C, Deesomchok A, Kositsakulchai W, Chalermpanchai N Tags: Jpn J Infect Dis Source Type: research

Clostridium difficile PCR ribotype 027 appears in Taiwan.
This report is important for hospital and public health administration in Asia to be alert and closely monitor the emergence of this hypervirulent strain to avoid potential outbreaks occurred. PMID: 25672348 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Liao TL, Lin CF, Chiou CS, Shen GH, Wang J Tags: Jpn J Infect Dis Source Type: research

Rubella seroprevalence among the general population in Dongguan, China.
Abstract In order to assess immunity to rubella infection in Dongguan, China, we conducted a seroprevalence survey on rubella and measured rubella-specific IgG in serum samples by ELISA. A total of 1017 individuals aged 0-59 years were selected with a multistage cluster sampling. Among them, 904 (88.9%) were seropositive to rubella. Two groups (20-29 and ≥40 years) had seropositivity rates
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Lin W, Wang D, Xiong Y, Tang H, Liao Z, Ni J Tags: Jpn J Infect Dis Source Type: research

A Food-borne Outbreak of Gastroenteritis caused by different Salmonella serotypes in two universities,Xiamen, Fujian, China, 2012.
Abstract We investigated a diarrhea outbreak in two universities to identify the etiological agent of the outbreak, the source of infection, the mode of transmission and the risk factors. A case-control study was conducted between the case-students and the asymptomatic control-students who were selected randomly and were frequency matched by class and age, and the source of food or water investigated. 0.25% of total 22,404 students (excluding teachers) developed Salmonella. 96% (54/56) of case-students and 30% (35/117) of control-students consumed bread products provided by the same vendor (odds ratio (OR)=63.3, 9...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Guo Z, Su CH, Huang J, Niu J Tags: Jpn J Infect Dis Source Type: research

Epidemiological characteristics of rubella and congenital rubella syndrome, in the 2012-2013 epidemics in Tokyo, Japan.
Abstract A large rubella outbreak has been observed since June 2012 in Tokyo, Japan, and rapid growth of congenital rubella syndrome (CRS) cases have also been reported in Japan since October 2012. All of the clinically diagnosed and laboratory-confirmed rubella cases reported in Tokyo from January 2012 to December 2013 and of the laboratory-confirmed CRS cases from January 2012 to March 2014 were analysed. A total of 4,116 rubella cases were reported in Tokyo. About three quarters were male (n = 3,176; 77.2%); the highest number of cases in male occurred at aged 35-39-year-old and in female 20-24-year-old. Compli...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Sugishita Y, Shimatani N, Katow S, Takahashi T, Hori N Tags: Jpn J Infect Dis Source Type: research

Molecular epidemiological analysis of Kudoa septempunctata by random amplified polymorphic DNA analysis.
Abstract A molecular epidemiological analysis of Kudoa septempunctata isolates from 34 olive flounders associated with foodborne disease outbreaks and 6 reference samples was performed using random amplified polymorphic DNA (RAPD) analysis. The K. septempunctata isolates analyzed in this study were divided into 8 groups. The 8 isolates obtained from the large Ehime prefecture outbreak in Japan that occurred on October 8, 2010 were divided into 4 groups. The 8 isolates obtained from Korean samples were divided into 3 groups that included isolates that had been identified from the large Ehime prefecture outbreak. Th...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Ohnishi T, Furusawa H, Oyama R, Koike S, Yoshinari T, Kamata Y, Sugita-Konishi Y Tags: Jpn J Infect Dis Source Type: research

ABO blood groups and susceptibility to brucellosis.
Abstract The relationship between blood groups and some infections like norovirus, cholera, and malaria has been reported. Given the importance of brucellosis and the failure to address this issue in the case of brucellosis, in this study we examined the relationship between blood groups and brucellosis. In this case-control study, 100 patients with brucellosis and 200 healthy individuals were studied in terms of blood groups. Exclusion criteria for the control group consisted of positive Coombs Wright or the history of brucellosis. Chi-square test was used to compare qualitative variables between the two groups. ...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Mohsenpour B, Hajibagheri K, Afrasiabian S, Ghaderi E, Ghasembegloo S Tags: Jpn J Infect Dis Source Type: research

Interleukin-17A in Legionella pneumonia: A retrospective study.
Abstract IL-17A is involved in the immune system of the lung. Legionella can potentially cause severe pneumonia. The present study was aimed to evaluate the role of IL-17A in Legionella pneumonia cases. IL-17A was quantified in sera from patients with Legionella pneumonia and control subjects. IL-17A was detected in sera from 4 out of 31 Legionella pneumonia cases; the cytokine was not detected in any control cases. There was no difference in peripheral white blood cell counts or other serum biomarkers (C-reactive protein, and lactate dehydrogenase) between IL-17A positive cases and IL-17A negative cases. The IL-1...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Higa F, Haroon A, Iha Y, Tasato D, Nakamura H, Kinjo T, Tamayose M, Furugen M, Miyagi K, Haranaga S, Tateyama M, Fujita J Tags: Jpn J Infect Dis Source Type: research

Genotype Diversity and Quasispecies Development of Helicobacter pylori in a Single Host.
Abstract Infection with different strains of H. pylori and emergence of new genomic variants during their long term gastric colonization are assumed as main reasons for eradication failure. This project was designed to detect relatedness and genetic variations among the H. pylori single isolates from each patient in Iran. Genotyping and arbitrarily primed PCR fingerprinting (RAPD) were used in this regard. Multiplex-PCR amplification of gene alleles encoding virulence factors vacA (m/s), cagA, iceA (A1/A2) and comparison of RAPD patterns of different singles colonies were performed for each individual patient. The...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Farzi N, Malekian T, Alebouyeh M, Vaziri F, Zali MR Tags: Jpn J Infect Dis Source Type: research

Contribution of QnrA, plasmid-mediated quinolone resistance peptide, to survival of Escherichia coli exposed to lethal ciprofloxacin concentration.
Abstract We evaluated the effects of qnrA gene on (1) survival of bacteria exposed to lethal ciprofloxacin (CIP) concentration, and (2) the development of quinolone resistance through the accumulation of amino acid substitutions in quinolone resistance-determining regions (QRDRs) of GyrA and ParC, targets of quinolones, in Escherichia coli. CIP-susceptible E. coli strains of different O-serotypes (O1, O6, O18, O25b, O74, and O78) were transformed by a recombinant plasmid harboring qnrA, and the parent strains and their transformants were subjected to killing curve assays and adaptation tests. In the killing curve ...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Goto K, Kawamura K, Arakawa Y Tags: Jpn J Infect Dis Source Type: research

Lactococcus lactis catheter-related bloodstream infection in an infant: case report.
LACTOCOCCUS LACTIS CATHETER-RELATED BLOODSTREAM INFECTION IN AN INFANT: CASE REPORT. Jpn J Infect Dis. 2014 Dec 24; Authors: Karaaslan A, Soysal A, Sarmış A, Kadayifci EK, Cerit K, Atıcı S, Söyletir G, Bakır M Abstract Lactococcus lactis is a gram-positive coccus that is nonpathogenic in humans. Herein, we present the case of a one-year-old boy with Down's syndrome (DS) and Hirschprung's disease (HD) who developed a catheter-related bloodstream infection (CR-BSI) with L. lactis after gastrointestinal surgery. The patient had been hospitalized in the pediatric surgery unit from birth because ...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Karaaslan A, Soysal A, Sarmış A, Kadayifci EK, Cerit K, Atıcı S, Söyletir G, Bakır M Tags: Jpn J Infect Dis Source Type: research

New Validated Rapid Screening Methods for Identifying Kudoa septempunctata in Olive Flounder (Paralichthys olivaceus).
Abstract Kudoa septempunctata is a new causative agent of foodborne diseases associated with the consumption of raw olive flounder. Qualitative PCR and quantitative real-time PCR have been developed as notification methods to detect K. septempunctata in Japan; however, these methods require expensive equipment and are time-consuming, taking two to three hours. In order to address these problems, in this study, we developed new rapid and simple methods using real-time LAMP and NASBA-nucleic acid chromatography (NASBA-NAC). In these methods, the total procedure requires approximately 45 minutes, without the need for...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Sugita-Konishi Y, Fukuda Y, Mori KI, Mekata T, Namba T, Kuroda M, Yamazaki A, Ohnishi T Tags: Jpn J Infect Dis Source Type: research

Description of hospitalized cases of influenza A(H1N1)pdm09 infection -based on the national hospitalized-case surveillance, 2009-2010, Japan.
This study reports the epidemiological characteristics of hospitalized cases of influenza A(H1N1)pdm09 infection analysed based on the surveillance data collected from 24 July 2009, the date on which the hospital-based surveillance of influenza cases was implemented in Japan, to 5 September 2010. During the study period, a total of 13,581 confirmed cases was reported. Among those cases with information regarding reason for hospitalization, 39% were admitted to hospitals for non-therapeutic purpose such as medical observation and laboratory testing. Overall hospitalization rate was 5.8 cases per 100,000 population when we e...
Source: Japanese Journal of Infectious Diseases - December 24, 2014 Category: Infectious Diseases Authors: Shimada T, Sunagawa T, Taniguchi K, Yahata Y, Kamiya H, Ueno Yamamoto K, Yasui Y, Okabe N, for the National Pandemic Response and Control Headquarters of Ministry of Health, Labour and Welfare, Japan Tags: Jpn J Infect Dis Source Type: research

Application of the analytic hierarchy process to a risk assessment of emerging infectious diseases in shaoxing city in southern china.
This study aimed to assess the likelihood of an outbreak or epidemic of emerging infectious diseases (EIDs) in Shaoxing city, China, and its resulting impact to provide decision makers with quantitative, directive results. Factors related to the risk of EIDs were selected through meeting with experts and were arranged in a hierarchical structure. These evaluation factors were also weighted to allow the use of a point system for evaluation. As a result, 14 evaluation factors comprising a 3-layer hierarchy were generated. The riskiest top 10 EIDs were HIV/AIDS (consistency index [CI] = 3.206), cholera (CI = 3.103), SARS (CI ...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Tu C, Fang Y, Huang Z, Tan R Tags: Jpn J Infect Dis Source Type: research

Effects of ribavirin on severe Fever with thrombocytopenia syndrome virus in vitro.
We examined the effects of ribavirin and mizoribine, which are nucleoside analogue drugs with broad antiviral activities, on SFTSV proliferation in vitro. When 3 cell lines were treated with these drugs before and during infection with a Chinese SFTSV strain, the 99% effective concentrations (EC99) of ribavirin were 19-64 μg/ml (78-262 μM); in contrast, the EC99 of mizoribine was>500 μg/ml (1,929 μM). Similar levels of inhibitory effects of ribavirin were observed with 4 Japanese SFTSV strains. However, when Vero cells were treated with ribavirin 3 days after inoculation, the inhibitory effect was dramatical...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Shimojima M, Fukushi S, Tani H, Yoshikawa T, Fukuma A, Taniguchi S, Suda Y, Maeda K, Takahashi T, Morikawa S, Saijo M Tags: Jpn J Infect Dis Source Type: research

Validation of thwaites' diagnostic scoring system for the differential diagnosis of tuberculous meningitis and bacterial meningitis.
Abstract To compare the clinical features of patients with tuberculous meningitis (TBM) and bacterial meningitis (BM) and to validate Thwaites' diagnostic scoring system for the differential diagnosis of TBM and BM, a retrospective review of 211 patients with TBM or BM who were admitted to Huashan Hospital, Fudan University, from 2007 to 2012 was conducted. The clinical characteristics and laboratory data were compared, and Thwaites' diagnostic scores were assessed at the time of admission for the differential diagnosis of TBM and BM. Significant differences were observed between the 2 groups in general informatio...
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Zhang YL, Lin S, Shao LY, Zhang WH, Weng XH Tags: Jpn J Infect Dis Source Type: research

Lack of Association between BSG Polymorphisms and Cerebral Malaria.
In conclusion, common SNPs in BSG do not influence the risk of cerebral malaria in the Thai population. PMID: 25410557 [PubMed - in process] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 26, 2014 Category: Infectious Diseases Authors: Naka I, Patarapotikul J, Hananantachai H, Ohashi J Tags: Jpn J Infect Dis Source Type: research