Serotypes, pathogenic potential and antimicrobial resistance of Escherichia coli isolated from subclinical bovine mastitis milk samples in Egypt.
In this study, E. coli were isolated from fourteen (9.3%) SCM milk samples. The isolated E. coli (n=14) were serotyped, their potential pathogenicity and antimicrobial resistances (AMRs) were investigated. Serotyping results showed that the E. coli isolates belonged to the O55:H7 (n=2), O111:H4 (n=2), O127:H6 (n=2), O128:HUN (n=2), O26:HUN (n=1), O44:H18 (n=1), O114:H21 (n=1), O86:HUN (n=1), O124:HUN (n=1) and O127:H7 (n=1) serogroups. Potential pathogenicity was detected in 93% (13/14) of the isolates. In particular, thirteen isolates possessed at least one of the examined virulence genes. Ten isolates (71%) exhibited AMR...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Ombarak RA, Zayda MG, Hinenoya A, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Shift to older ages of HIV/AIDS deaths and its gender difference: inference derived from Vital Statistics of Japan.
Abstract Death of HIV/AIDS patients 55 years old started increasing. Though deaths directly linked to infections declined since 2005, those related to malignancy, encephalopathy, interstitial pneumonia, wasting syndrome, etc. persisted. For all of HIV/AIDS (mainly males), ATL, CJD and general population, age of death shifted to older ages by 5 years in 10 years from 1999-2004 to 2010-2017. Among them, only HIV/AIDS patients and general population exhibited unequivocal gender difference. As of 2011-2016, the median of the deaths of the HIV/AIDS patients was 52.5 years for males and 70 years for females, while the m...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research

Rapid identification and quantification of Lactobacillus rhamnosus-targeting real-time PCR using a TaqMan probe.
In this study, we developed an L. rhamnosus-targeting quantitative PCR assay, which produces accurate and reproducible results based on the specificity of a TaqMan probe targeting the unique 16S rDNA sequence of L. rhamnosus. The assay specifically detected the targeted bacterium, L. rhamnosus, and no non-specific signals were generated under the study conditions. Using genomic DNA from the bacterial cells of L. rhamnosus (101 to 106 cells), the cycle threshold value showed a linear trend (R2 = 0.9993). This L. rhamnosus-targeting quantitative PCR assay can contribute to advance research into the effects of the organism on...
Source: Japanese Journal of Infectious Diseases - April 26, 2019 Category: Infectious Diseases Authors: Okai C, Itani Y, Furuta A, Mizunoe Y, Iwase T Tags: Jpn J Infect Dis Source Type: research

First report of TEM-116 and OXA-10 extended-spectrum β-lactamase in clinical isolates of Alcaligenes species from Kuala Lumpur, Malaysia.
First report of TEM-116 and OXA-10 extended-spectrum β-lactamase in clinical isolates of Alcaligenes species from Kuala Lumpur, Malaysia. Jpn J Infect Dis. 2019 Mar 26;: Authors: Puah SM, Puthucheary SD, Chua KH Abstract There is an alarming increase of extended-spectrum β-lactamases (ESBL) being present mainly in Enterobacteriaceae and other non-fermenting gram-negative bacteria, such as Alcaligenes faecalis which is the only species present in that genus that is clinically relevant. We investigated Alcaligenes species from 7 cases (6 inpatients and 1 outpatient) at our tertiary hospital. F...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Puah SM, Puthucheary SD, Chua KH Tags: Jpn J Infect Dis Source Type: research

Poor sleep quality is associated with higher risk of pulmonary tuberculosis among patients with type 2 diabetes mellitus course more than 5 years.
In conclusion, poor sleep quality is an independent risk factor of PTB among DM patients with course>5 years, which indicates significant epidemiological implications for PTB control. PMID: 30918145 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Kou T, Wang Q, Lv W, Wei B, Liu Y, Zhao S, Du X, Zou Y, Sun L, Tian H, Zhao Z, Liu Q, Dong C, Zhang G, Gao X, Yu C Tags: Jpn J Infect Dis Source Type: research

Enhancing preparedness against rubella at the workplace: proactive prevention efforts by the Tokyo Metropolitan Government.
Abstract During Japan's 2012-2013 rubella epidemic, most transmission in Tokyo occurred at workplaces, especially among men aged 20 to 49. This tendency was likely related to previous rubella immunization policies that prioritized female adolescents. Since 2015, the Tokyo Metropolitan Government has been promoting a project to prevent rubella and other infectious diseases in workplaces. Companies participate by choosing one or more of three options: (I) Acquire fundamental understanding of infectious diseases; (II) Develop a Business Continuity Plan (BCP) for infectious diseases in the workplace; or (III) Increase...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Sugishita Y, Soejima K, Kayebeta A, Yauchi M Tags: Jpn J Infect Dis Source Type: research

Epidemiologic Trends and Clinical Features of Pneumocystis jirovecii Pneumonia in Non-HIV Patients in a Tertiary-Care Hospital in Korea over a 15-Year-Period.
Abstract Subsequent to the increasing use of immunosuppressant therapy, Pneumocystis jirovecii pneumonia (PcP) has emerged as a life-threatening condition in human immunodeficiency virus (HIV)-negative patients. We investigated changes in epidemiological and clinical characteristics among PcP cases with and without HIV infections. Data of 424 patients diagnosed with PcP in a 2,700-bed Korean tertiary care hospital between February 2003 and April 2017 were retrospectively analyzed. The study included patients with compatible clinical findings in whom PcP was confirmed via direct immunofluorescence assay. The annual...
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Lee HY, Choi SH, Kim T, Chang J, Kim SH, Lee SO, Kim MN, Sung H Tags: Jpn J Infect Dis Source Type: research

Clinical evaluation of a newly developed chemiluminescent enzyme immunoassay in Japan for hepatitis C virus core antigen.
In conclusion, LP-Presto shows rapid, reliable performance. Nonspecific testing results due to hyperimmunoglobulinemia were reduced by LP-Presto. LP-Presto is a high-quality HCVcAg assay that shows promise for various applications. PMID: 30918148 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 26, 2019 Category: Infectious Diseases Authors: Inoue T, Ohike T, Ohne K, Sato S, Goto T, Tanaka Y Tags: Jpn J Infect Dis Source Type: research

Predominance of ST22-MRSA-IV clone and Emergence of Clones for Methicillin-resistant Staphylococcus aureus clinical isolates collected from a Tertiary Teaching Hospital over a two-year period.
This study aimed to determine the genotypic and phenotypic characteristics of clinical MRSA isolates collected from a teaching hospital from 2014 - 2015. These isolates were genotyped by multilocus sequence typing (MLST), staphylococcal cassette chromosomal mec (SCCmec) typing, virulence genes detection and pulsed-field gel electrophoresis (PFGE) and phenotyped based on their vancomycin susceptibility profiles. The most prevalent sequence type was ST22. ST3547 was identified from a blood isolate from 2015. Three SCCmec types (III in 26.26%, IV in 70.71% and V in 3.03%) were detected. Agr type I, II, III were detected among...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Niek WK, Teh CSJ, Idris N, Thong KL, Ponnampalavanar S Tags: Jpn J Infect Dis Source Type: research

Genome-based epidemiological analysis of 13 Acinetobacter strains isolated from blood cultures of hospitalized patients from a university hospital in Japan.
In this study, we aimed to characterize 13 Acinetobacter strains isolated from blood cultures from Osaka City University Hospital. We conducted whole-genome sequencing to reveal their genetic background. We also performed PCR-based open reading frame typing (POT) and compared the results with those of multilocus sequence typing (MLST) to confirm its reliability as a genotyping method. Although biochemical analysis suggested that most isolates were A. baumannii, genomic analysis revealed that the collection of Acinetobacter isolates comprised six different species, with non-baumannii Acinetobacter species representing the m...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Oinuma KI, Suzuki M, Nakaie K, Sato K, Saeki K, Sakiyama A, Takizawa E, Niki M, Niki M, Yamada K, Shibayama K, Kakeya H, Kaneko Y Tags: Jpn J Infect Dis Source Type: research

Evaluation of Modified Sequential Multiplex PCR for Streptococcus pneumoniae Serotyping.
Abstract The aims of this study were to develop modified SM-PCR primer sets and to evaluate their ability and efficiency for serotype determination. We selected target serotypes for SM-PCR testing according to the prevalence of serotypes as reported in papers published in Asian countries. The modified SM-PCR consisted of six groups of PCRs, with each reaction being carried out using five pairs of primers. We evaluated the efficiency and performance of this modified multiplex PCR using 378 pneumococcal strains by comparing the findings with the results of the Quellung reaction. A total of 30 primer pairs were used ...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Park D, Kim SH, Bae IK, Kim NY, Kook JK, Park YH, Shin JH Tags: Jpn J Infect Dis Source Type: research

Comparison of clinical characteristics of human metapneumovirus and respiratory syncytial virus infection in hospitalized young children.
Abstract Human metapneumovirus (HMPV) infection and respiratory syncytial virus (RSV) infection are the leading causes of acute respiratory tract infection in children, and clinical manifestations of these virus infections are considered similar. To investigate the differences in clinical characteristics between HMPV and RSV infections in young children, we prospectively enrolled children
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Taniguchi A, Kawada JI, Go K, Fujishiro N, Hosokawa Y, Maki Y, Sugiyama Y, Suzuki M, Tsuji T, Hoshino S, Muramatsu H, Kidokoro H, Kinoshita F, Hirakawa A, Takahashi Y, Sato Y, Natsume J Tags: Jpn J Infect Dis Source Type: research

Characterization of an unusual DS-1-like G8P[8] rotavirus strain from Japan in 2017: Evolution of emerging DS-1-like G8P[8] strains through reassortment.
Abstract The emergence of unusual DS-1-like intergenogroup reassortant rotaviruses having the bovine-like G8 genotype (DS-1-like G8P[8] strains) has been reported from several Asian countries. During the rotavirus surveillance program in Japan in 2017, a DS-1-like G8P[8] strain (RVA/Human-wt/JPN/SO1162/2017/G8P[8]) was identified in 43 rotavirus-positive stool samples. Strain SO1162 was shown to have a unique genotype constellation including both genogroup 1 and 2 genes: G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that the VP1 gene of strain SO1162 appeared to have originated from DS-1-like ...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Kamiya H, Tacharoenmuang R, Ide T, Negoro M, Tanaka T, Asada K, Nakamura H, Sugiura K, Umemoto M, Kuroki H, Ito H, Tanaka S, Ito M, Fukuda S, Hatazawa R, Hara Y, Guntapong R, Murata T, Taniguchi K, Suga S, Nakano T, Taniguchi K, Komoto S Tags: Jpn J Infect Dis Source Type: research

Analysis of the type II-A CRISPR-Cas system in Streptococcus canis isolated from diseased companion animals and one human patient in Japan.
Abstract We determined the whole genome sequence (WGS) of Streptococcus canis strain TA4 harboring the M-like protein gene (scm), which was isolated from a human patient presenting with bacteremia. The potential of type II-A CRISPR array-based typing was explored and the genetic lineage between spacer genogroups and scm prevalence/polymorphisms in the isolates from 19 diseased companion animals and the human patient was elucidated. The CRISPRFinder/CRISPRCasFinder detected the type II-A locus with the same repeat sequences in TA4 and another WGS of Sc isolated from a cow with mastitis. An optimized PCR-based ampli...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Yoshida H, Fukushima Y, Goto M, Tsuyuki Y, Takahashi T Tags: Jpn J Infect Dis Source Type: research

Longitudinal epidemiology of viral infectious diseases combining virus isolation, antigenic analysis and phylogenetic analysis as well as seroepidemiology in Yamagata, Japan between 1999 and 2018.
Abstract We introduced a microplate method for virus isolation in the Department of Microbiology, Yamagata Prefectural Institute of Public Health (YPIPH) in 1999 in Yamagata, Japan. We have since carried out longitudinal epidemiological studies of viral infectious diseases, particularly, respiratory viruses, combining traditional technologies such as virus isolation and serological techniques and newly developed molecular methods. Here, we provide an overview of our activities at YPIPH between 1999 and 2018. During the study period, we observed emerging and re-merging diseases such as echovirus type 13, enteroviru...
Source: Japanese Journal of Infectious Diseases - February 28, 2019 Category: Infectious Diseases Authors: Mizuta K, Tanaka W, Komabayashi K, Tanaka S, Seto J, Aoki Y, Ikeda T Tags: Jpn J Infect Dis Source Type: research

Effects of antimicrobial administration on the prevalence of antimicrobial-resistant Escherichia coli in broiler flocks.
Abstract Increase of antimicrobial-resistant (AMR) bacteria caused by antimicrobial usage is a public health problem. We investigated the proportion of cephalexin (LEX)-resistant bacteria in fresh feces obtained from antimicrobial-free broilers in three flocks at 40 days of age. DHL agar plates containing 25 μg/mL LEX (DHL-L) showed LEX-resistant bacteria in all flocks at 40 days of age. The bacterial counts on DHL and DHL-L were 105-108 colony forming units (CFU)/g feces and
Source: Japanese Journal of Infectious Diseases - January 31, 2019 Category: Infectious Diseases Authors: Suzuki K, Yossapol M, Sugiyama M, Asai T Tags: Jpn J Infect Dis Source Type: research

Prevalence of protective measles virus antibody levels in umbilical cord blood samples and sera of mothers and, transplacental transport ratio in Turkey.
Abstract In Turkey, Measles Elimination Programme has been implemented since 2002. The aim of this research was to evaluate the measles specific antibody levels of mothers who admitted to a hospital for birth and their infants, and to determine the factors influencing the antibody levels of both and to evaluate the transplacental transport ratio. We selected healthy women who came to the hospital for birth and their healthy newborns. We collected blood samples from 1547 mothers and 1529 infants.The protective prevalance of measles antibody levels of mothers was 85% (95%CI:78-82), and the newborns' was 85% (95%CI:8...
Source: Japanese Journal of Infectious Diseases - January 31, 2019 Category: Infectious Diseases Authors: Coban SC, Temel F, Duman P, Cosgun Y, Ozarslan F, Kayman T, Guven S, Bezirganoglu H, Gunes O, Yilmaz Ciftdogan D, Karadagli EE, Kaya C, Kara A Tags: Jpn J Infect Dis Source Type: research

Assessment of the 24th week success of anti-retroviral therapy in ACTHIV-IST Database: Results from a region with increasing incidence.
Abstract We aimed to assess 24-week virological and immunological success of treatment naive and experienced patients included in ACTHIV-IST database. ACTHIV-IST database were screened retrospectively from January 2012 to January 2014. Data of these patients such as age, sex, being treatment naive or experienced, date of diagnosis, date of commencing antiretroviral therapy, antiretroviral therapy regimen, CD4+ cell count and viral load before and after therapy were analyzed. In the 24th week of antiretroviral therapy, virological and immunological failures were 40 (17.9%) and 29 (14.1%), respectively. Virological ...
Source: Japanese Journal of Infectious Diseases - January 31, 2019 Category: Infectious Diseases Authors: Bolukcu S, Mete B, Gunduz A, Karaosmanoglu HK, Sargin F, Durdu B, Aydin OA, Yildiz D, Dokmetas I, Aslan T, Tabak F Tags: Jpn J Infect Dis Source Type: research

Subclinical infections of Anaplasma phagocytophilum and Anaplasma bovis in dogs in Ibaraki, Japan.
Abstract The prevalence of Anaplasma infection in 332 dogs from Ibaraki, Japan, was evaluated by serological and molecular surveys. Immunofluorescence antibody assay (IFA) against Anaplasma phagocytophilum indicated that 7 (2.1%) of 328 dogs were positive for A. phagocytophilum. Screening PCR demonstrated that 8 (2.4%) of 331 dogs were positive for Anaplasmataceae. Phylogenetic analysis of the partial 16S rRNA sequence of PCR amplicons revealed that 6 sequences were most similar to the 16S rRNA sequence of Wolbachia sp. and the remaining 2 to Anaplasma bovis. Further analysis by A. phagocytophilum-specific nested ...
Source: Japanese Journal of Infectious Diseases - January 31, 2019 Category: Infectious Diseases Authors: Fukui Y, Inokuma H Tags: Jpn J Infect Dis Source Type: research

Molecular detection and characterization of p44/msp2 multigene family of Anaplasma phagocytophilum from Haemaphysalis longicornis in Mie Prefecture, Japan.
In this study, we further investigated total 483 H. longicornis ticks (220 adults and 263 nymphs) collected in Mie Prefecture by p44/msp2 targeting PCR assay for the characterization of p44/msp2 multigene family of A. phagocytophilum in Japan. Six of 483 ticks tested were PCR-positive for A. phagocytophilum p44/msp2 and these positive individuals were all nymphs at tick life stage. Cloning, sequence, and phylogenetic analyses of the amplicons revealed that 11 p44/msp2 clones obtained shared the similarities of 54.9-99.3 % with 27 previously-identified clones from HGA patients in Japan based on amino acid sequences, especia...
Source: Japanese Journal of Infectious Diseases - January 31, 2019 Category: Infectious Diseases Authors: Su H, Sato A, Onoda E, Fujita H, Sakabe S, Akachi S, Oishi S, Abe F, Kanda T, Shimamura Y, Masuda S, Ohashi N Tags: Jpn J Infect Dis Source Type: research

Targets for optimizing oral antibiotic prescriptions for pediatric outpatients in Japan.
Abstract In Japan, 92.6% of antibiotics consumed are oral agents, most of which are for outpatients. A significant proportion is known to be dispensed for children; however, the specific pattern of antibiotic prescribing according to clinical specialty is still unclear. The aim of our study was to identify key targets for optimizing oral antibiotic use in children. We analyzed data on oral antimicrobial prescription patterns for children aged
Source: Japanese Journal of Infectious Diseases - December 25, 2018 Category: Infectious Diseases Authors: Uda K, Kinoshita N, Morisaki N, Kasai M, Horikoshi Y, Miyairi I Tags: Jpn J Infect Dis Source Type: research

Clinical and Laboratory Findings of MERS-CoV infection.
Abstract There is a paucity of data regarding the differentiating characteristics of patients with laboratory confirmed and those negative for Middle East respiratory syndrome coronavirus (MERS-CoV) in South Korea. This is a hospital-based retrospective study comparing MERS-CoV-positive patients with MERS-CoV-negative patients. A total of 7 positive patients and 55 negative patients with a median age of 43 (P = 0.833) were included. No statistical differences were observed in relation to sex and the presence of comorbidities. At the time of admission, headache (28.6% vs 3.6%; OR, 10.60; 95% CI, 1.22-92.27), myalgi...
Source: Japanese Journal of Infectious Diseases - December 25, 2018 Category: Infectious Diseases Authors: Hwang SM, Na BJ, Jung YM, Lim HS, Seo JE, Park SN, Cho YS, Song EH, Seo JY, Kim SR, Lee GY, Kim SJ, Park YS, Seo HS Tags: Jpn J Infect Dis Source Type: research

Nested polymerase chain reaction with specific primers for Mucorales in the serum of patients with hematological malignancies.
We present two cases in which nested polymerase chain reaction with specific primers was used in the serum of patients with hematological malignancies. PMID: 30584197 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - December 25, 2018 Category: Infectious Diseases Authors: Hirano M, Ota Y, Koibuchi T, Takei T, Takeda R, Kawamata T, Yokoyama K, Uchimaru K, Yotsuyanagi H, Imai Y, Tojo A Tags: Jpn J Infect Dis Source Type: research

A comparative review of prevention of rabies incursion between Japan and other rabies-free countries or regions.
Abstract Although rabies still kills many people, global eradication of human rabies is considered to be feasible. Because the progress toward eradication may be different among regions with different socio-economic status, states that successfully eradicate this disease must be vigilant for re-emergence of rabies. In this article, we describe arguments over current rabies prevention measures and risk assessment results for rabies introduction and spread in rabies-free Japan. We also summarize the measures taken by representative countries and regions free from rabies. Our risk assessment results revealed that the...
Source: Japanese Journal of Infectious Diseases - December 25, 2018 Category: Infectious Diseases Authors: Yamada A, Makita K, Kadowaki H, Ito N, Sugiyama M, Kwan NCL, Sugiura K Tags: Jpn J Infect Dis Source Type: research

Kluyvera ascorbata as a Pathogen in Adults and Children: Clinical Features and Antibiotic Susceptibilities in a Single Center Study.
Abstract To approach the clinical characteristics of rare Kluyvera ascorbata infection, we reviewed the medical records of patients from whom K. ascorbata was isolated from 2010 to 2016, and conducted a systematic review of the English and Spanish literature in PubMed for reports of K. ascorbata infection in humans from 1971 to 2018. A total of 43 cases (24 adults and 19 children) were enrolled: three in our hospital and 40 from literature review. The urinary tract was the most common site of infection (44.2%, 19/43), followed by the bloodstream (27.9%, 12/43). There was no significant difference in frequency of u...
Source: Japanese Journal of Infectious Diseases - November 30, 2018 Category: Infectious Diseases Authors: Lee J, Hwang JH, Jo DS, Lee HS, Hwang JH Tags: Jpn J Infect Dis Source Type: research

Isolation of a Capnophilic and an Extended-Spectrum- β-Lactamase-Producing Proteus mirabilis Isolate with Inability to Grow under pH-Indicator Dyes from the Urine of an Octogenarian Male-Patient with Acute Pyelonephritis.
Isolation of a Capnophilic and an Extended-Spectrum-β-Lactamase-Producing Proteus mirabilis Isolate with Inability to Grow under pH-Indicator Dyes from the Urine of an Octogenarian Male-Patient with Acute Pyelonephritis. Jpn J Infect Dis. 2018 Nov 30;: Authors: Soga E, Akiyama M, Ohsaki Y, Hayashi W, Matsumoto T, Oana K, Nagano N, Kawakami Y Abstract A capnophilic Gram-negative rod was recovered from the urine of an octogenarian male patient with acute pyelonephritis. The isolate also demonstrated to produce CTX-M-2-type- extended-spectrum-β-lactamase. Interestingly, the isolate failed to gr...
Source: Japanese Journal of Infectious Diseases - November 30, 2018 Category: Infectious Diseases Authors: Soga E, Akiyama M, Ohsaki Y, Hayashi W, Matsumoto T, Oana K, Nagano N, Kawakami Y Tags: Jpn J Infect Dis Source Type: research

Careful clinical surveillance is important for the identification of human parechovirus type A3-associated myalgia/myositis: a sporadic case found in a season with a low level of its activity in Yamagata, Japan in 2017.
PMID: 30504645 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - November 30, 2018 Category: Infectious Diseases Authors: Tanaka S, Sendo D, Ichikawa M, Toyota K, Furuyama M, Komabayashi K, Ikeda T, Mizuta K Tags: Jpn J Infect Dis Source Type: research

Study on Procedure for Lot Release of Vaccines in Japan.
Abstract Biological products, such as vaccines, blood products, antitoxin, and anti-venom, are released on the market following a lot release conducted by National Regulatory Authorities or National Control Laboratories, even if their manufacturing and marketing have been authorized. Independent lot release by regulatory authorities is not a unique procedure to Japan, but rather a common step worldwide. Previously Japan has carried out lot release mainly by laboratory tests, and the record of manufacturers' in-house tests was used as a reference, not involved in the decision of lot release. Conversely, the interna...
Source: Japanese Journal of Infectious Diseases - November 30, 2018 Category: Infectious Diseases Authors: Kato A, Fujita K, Ochiai M, Naito S, Konda T Tags: Jpn J Infect Dis Source Type: research

Predomination shift of different P44-expressing Anaplasma phagocytophilum in infected HL-60, THP-1, NB4, and RF/6A cell lines.
In this study, we performed RNA-seq to investigate P44 expression of A. phagocytophilum propagated in 4 different cell lines. In infected HL-60 cells, P44-2b transcript was predominantly detected in first RNA-seq analysis (HL-60.1), but P44-23 transcript became predominated in second RNA-seq analysis at 1 month after additional passages (HL-60.2). We further analyzed P44 expression of A. phagocytophilum cultured in THP-1, NB4, and RF/6A cells through consecutively-repeated passages with own cell line for 1 year after transfer of A. phagocytophilum from infected HL-60 to respective cell lines. In those long-term cultures, P...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Shimada M, Takamoto N, Su H, Sasahara H, Shimamura Y, Ando S, Ohashi N Tags: Jpn J Infect Dis Source Type: research

Loop-mediated isothermal amplification for rapid identification of Mycobacterium tuberculosis in comparison with immunochromatographic SD Bioline MPT64 Rapid ® in a high burden setting.
Loop-mediated isothermal amplification for rapid identification of Mycobacterium tuberculosis in comparison with immunochromatographic SD Bioline MPT64 Rapid® in a high burden setting. Jpn J Infect Dis. 2018 Oct 31;: Authors: Phetsuksiri B, Rudeeaneksin J, Srisungngam S, Bunchoo S, Klayut W, Sangkitporn S, Nakajima C, Hamada S, Suzuki Y Abstract Loop-mediated isothermal amplification (LAMP) was assessed for rapid identification of Mycobacterium tuberculosis complex (MTC) in comparison with an immunochromatographic test (ICT) using SD Bioline Ag MPT64 Rapid®. One hundred and fifty-one MGIT cult...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Phetsuksiri B, Rudeeaneksin J, Srisungngam S, Bunchoo S, Klayut W, Sangkitporn S, Nakajima C, Hamada S, Suzuki Y Tags: Jpn J Infect Dis Source Type: research

Detection of Uncommon Enteric Bacterial Pathogens from Acute Diarrheal Specimens using SYBR-Green Real Time PCR.
We examined SYBR-Green real-time PCR for the detection of ten uncommon bacterial pathogens using fecal specimens from acute diarrheal patients. In SYBR-Green real-time PCR, the products formed were identified based on melting point temperature (Tm) curve analysis and was validated with the respective reference strain. In a retrospective study, 1184 tested stool specimens by conventional culture methods were included. Enterotoxigenic Bacteriodes fragilis was detected more (6.7%) followed by enterotoxigenic Bacillus cereus (5.1%), Clostridium perfringens (3.9%) and Aeromonas hydrophila (3.8%). In the prospective study, A. hy...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Chowdhury G, Stine OC, Rajendran K, Mukhopadhyay AK, Okamoto K, Ramamurthy T Tags: Jpn J Infect Dis Source Type: research

Possible reintroduction of Taenia asiatica into the Kanto region of Japan.
PMID: 30381679 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Taniyama D, Inoue I, Kawano M, Arakawa C, Adachi T, Morishima Y, Yamasaki H, Sugiyama H Tags: Jpn J Infect Dis Source Type: research

Assessment of Bacteremia in a Large Tertiary Care Hospital in Northern Vietnam: A Single-center Retrospective Surveillance Study.
Abstract The clinical analysis of cases of Bacteremia would be valuable. However, thus far, limited data on bacteremia are available in Vietnam. A single-center, retrospective surveillance study was conducted in Bach Mai Hospital, Hanoi, Vietnam from 2009 to 2012. In total, 45,366 blood culture cases were analyzed. The number of blood cultures per 1,000 patient-days was 9.59 sets. The percentage of solitary blood culture sets was 49.6%. The rate of positive blood culture was 13.9%. The major pathogens isolated in adults were coagulase-negative Staphylococcus species (16.7%), followed by Escherichia coli (6.8%), St...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Takeshita N, Anh NQ, Phuong DM, Thanh DV, Thuy PP, Huong MTL, Takahashi M, Ohmagari N Tags: Jpn J Infect Dis Source Type: research

Species Identification of β-hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017).
This study determined species identification and antimicrobial resistance (AMR) properties in β-hemolytic streptococci isolated from companion animals in Japan. Strains were isolated from clinical specimens of 131 companion animals with symptoms/signs in April-May, 2017. We identified strains by 16S rRNA sequencing and antimicrobial susceptibility was determined using the broth microdilution method. AMR genes (erm(A)-erm(B)-mef(A) and tet(M)-tet(O)-tet(K)-tet(L)-tet(S)) in all isolates were amplified by PCR. The 16S rRNA sequencing identified β-hemolytic streptococcal species as Streptococcus canis (n = 117, 89.3...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Fukushima Y, Tsuyuki Y, Goto M, Yoshida H, Takahashi T Tags: Jpn J Infect Dis Source Type: research

Neutralization potency of sera from Vietnamese patients with Japanese encephalitis (JE) against genotypes I and V JE viruses.
In this study, we investigated the neutralization ability of the sera collected from 22 Vietnamese JE patients who lived in northern Vietnam against GI and GV JEV strains. In most cases, the ratios of the titer against GV to that against GI (GV:GI) were equal to or less than 1:4. However, the titer against GV JEV was equivalent (1:1) to that against GI JEV in only a few cases, and no serum had a ratio higher than 1:1. Thus, our results did not show convincing evidence that GV JEV have been emerging in northern Vietnam in 2014. PMID: 30381682 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Nguyen TTT, Tajima S, Ikeda M, Nguyen TT, Le THT, Pham TTH, Pham DQ, Le TQM, Maeki T, Taniguchi S, Kato F, Moi ML, Morita K, Lim CK, Saijo M, Hasebe F Tags: Jpn J Infect Dis Source Type: research

Effects of a Public Subsidization Program for Mumps Vaccine on Reducing the Disease Burden in Nagoya City, Japan.
Abstract Nagoya City initiated a public subsidization program for mumps vaccination using either Torii- or Hoshino-strains in August 2010. To demonstrate the effects of the program, we used publicly available information from Nagoya City to investigate the changes in immunization rate and numbers of patients who developed post-immunization adverse reactions, including post-vaccinal aseptic meningitis, in the 7 years since its initiation. We also investigated the numbers of mumps patients reported during this period by sentinel sites in a national database. The immunization rate in 1-year-old children increased fro...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Ozaki T, Goto Y, Nishimura N, Nakano T, Kumihashi H, Kano M, Ohfuji S Tags: Jpn J Infect Dis Source Type: research

The cost effective easy competitive ELISA (CEE-cELISA) available for monitoring protective immunity against the rabies virus in sera of humans and dogs.
Abstract The rabies vaccinations coverage has been reported as 70-80% of dogs in annual reports. However, there are still outbreaks of rabies in humans and dogs in Thailand, which indicate the necessity of ensuring seroprevalence in vaccinated dogs and efficacy of human immunization. The cost effective easy competitive ELISA (CEE-cELISA) was developed for monitoring protective immunity against the rabies virus in human and dog sera by using the monoclonal antibody clone 1-46-12, which recognized a conformation epitope of rabies G protein, with an ELISA plate coated with whole viral antigen of commercial vaccine. T...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Aronthippaitoon Y, Samer W, Atuntee T, Thananchai H, Thongkorn K, Pongsopawijit P, Inoue S, Noguchi A, Park ES, Kawai A, Petsophonsakul W Tags: Jpn J Infect Dis Source Type: research

An outbreak of rhinovirus species C infection in a welfare facility in Fukuoka Prefecture, Japan.
PMID: 30381685 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Ashizuka Y, Nakamura A, Yoshitomi H, Kobayashi T, Kajiwara J, Katsuki S, Kuroda M Tags: Jpn J Infect Dis Source Type: research

Infectious keratitis: microbiological review of 297 cases.
raetorius A Abstract Infectious keratitis is a serious ocular infection that can lead to loss of vision. The aim of the study was to investigate the microbiological characteristics of this infection at the University Hospital of Guadalajara (Spain). We retrospectively reviewed all cases diagnosed between January 2010 and December 2016. During the 7-year study period, 297 corneal scrapes corresponding to 298 patients were performed. Antibiotic treatment previous to the culture occurred in 59 cases (19.5%). Contact lens wear was the most common risk factor (33.2%). Bacterial keratitis accounted for 64.6% of cases, v...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Tena D, Rodríguez N, Toribio L, González-Praetorius A Tags: Jpn J Infect Dis Source Type: research

A novel recombinant norovirus GII.4 Sydney 2012 strain detected from a food poisoning outbreak in the 2017-2018 season, Japan.
PMID: 30381687 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Inasaki N, Aoyagi Y, Morioka S, Hasegawa S, Yoneda T, Saga Y, Itamochi M, Obuchi M Tags: Jpn J Infect Dis Source Type: research

Does quick Sepsis-related Organ Failure Assessment (qSOFA) suggest the use of initial empirical carbapenem therapy in bacteremia caused by extended-spectrum β-lactamase-producing bacteria? A multicenter case-control study.
Does quick Sepsis-related Organ Failure Assessment (qSOFA) suggest the use of initial empirical carbapenem therapy in bacteremia caused by extended-spectrum β-lactamase-producing bacteria? A multicenter case-control study. Jpn J Infect Dis. 2018 Oct 31;: Authors: Mitsuboshi S, Tsuruma N, Watanabe K, Takahashi S, Nakashita M, Ito A, Kobayashi K, Tsugita M Abstract We hypothesized that quick Sequential Organ Failure Assessment (qSOFA) would be associated with 30-day mortality in bacteremia caused by extended-spectrum β-lactamase (ESBL)-producing bacteria and might be a selection criterion for ...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Mitsuboshi S, Tsuruma N, Watanabe K, Takahashi S, Nakashita M, Ito A, Kobayashi K, Tsugita M Tags: Jpn J Infect Dis Source Type: research

Indication of Minimum Inhibitory Concentration of β-Lactam Antimicrobials for the Primary Extraction of IMP-Producing Enterobacteriaceae.
Indication of Minimum Inhibitory Concentration of β-Lactam Antimicrobials for the Primary Extraction of IMP-Producing Enterobacteriaceae. Jpn J Infect Dis. 2018 Oct 31;: Authors: Saito K, Nakamura K, Harada R, Nakano R, Yano H, Kanemitsu K PMID: 30381689 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Saito K, Nakamura K, Harada R, Nakano R, Yano H, Kanemitsu K Tags: Jpn J Infect Dis Source Type: research

Persistence of antibodies against diphtheria, tetanus, pertussis, and poliovirus types I, II, and III following immunization with DTaP combined with inactivated wild-type polio vaccine (DTaP-wIPV).
Abstract A follow-up serological study was conducted involving 47 subjects who received four doses of diphtheria and tetanus toxoids combined with acellular pertussis vaccine (DTaP) together with Salk-type inactivated polio vaccine (DTaP-wIPV) until 6 years of age. All antibody levels declined more rapidly than expected within 3 years after the completion of the primary vaccination with 4th booster dose and titers persisted until 6 years of age. The positive rate of the IgG antibody against pertussis toxin (PT) was 31.9% (15/47) at 4 years of age, 41.0% (16/39) at 5 years of age, and 40.5% (15/37) at 6 years of ag...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Nakayama T, Suga S, Okada K, Okabe N Tags: Jpn J Infect Dis Source Type: research

Comparison of established PCR assays for accurate identification of Campylobacter jejuni and Campylobacter coli.
This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The PCR assays had sensitivity of 85.2-100%, and 97-100%, and specificity of 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while, three assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problema...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Kabir SML, Chowdhury N, Asakura M, Shiramaru S, Kikuchi K, Hinenoya A, Neogi SB, Yamasaki S Tags: Jpn J Infect Dis Source Type: research

Invasive fungal infection caused by Geotrichum clavatum in an acute leukemia child: first documented case from mainland China.
Abstract Invasive fungal infections (IFIs) are one of the vital complications for acute leukemia patients undergoing induction chemotherapy. Among them, Geotrichum clavatum fungal infections present extremely rare with atypical clinical symptoms which make it difficult to diagnosis. In this paper, we report a case of infection caused by Geotrichum clavatum in a 10-year old acute leukemia child, which is the first documented case from mainland China. With childhood leukemia as the basic disease, the child suffered from repeated bacterial and fungal infection, and even underwent abdominal surgery during the treatmen...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Liu X, Zhou W, Jiang Y, Kuang L Tags: Jpn J Infect Dis Source Type: research

Development and evaluation of new real-time RT-PCR assays for identifying the influenza A virus Cluster IV H3N2 variant.
Abstract From 2005 to July 6, 2018, a total of 435 swine-origin influenza A H3N2 variant virus (H3N2v) human infections were reported in the USA. The largest H3N2v outbreak in the USA occurred in 2011-2012. This virus obtained the HA gene from human seasonal H3N2 influenza A viruses (seasonal H3N2) via human-to-swine transmission in the mid-1990s and was classified as Cluster IV H3N2v. For early detection of public health threats associated with Cluster IV H3N2v in distinction from seasonal H3N2, we developed highly specific and sensitive one-step real-time RT-PCR assays directly targeting the HA genes of Cluster ...
Source: Japanese Journal of Infectious Diseases - October 31, 2018 Category: Infectious Diseases Authors: Saito S, Nakauchi M, Takayama I, Nagata S, Odagiri T, Kageyama T Tags: Jpn J Infect Dis Source Type: research

Molecular Investigation of Dengue virus serotype 2 Circulation in Kassala State, Sudan.
This study urges the need for a wider investigation of the DENV serotypes composition and estimating their contribution to the ongoing transmission. PMID: 30270247 [PubMed - as supplied by publisher] (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - September 28, 2018 Category: Infectious Diseases Authors: Hamid Z, Hamid T, Alsedig K, Abdallah T, Elaagip A, Ahmed A, Khalid F, Abdel Hamid M Tags: Jpn J Infect Dis Source Type: research

Selective detection of DNA of viable   Mycobacterium tuberculosis complex strains using EMA-PCR method.
In this study, the ability to discriminate viable cells from dead cells of Mycobacterium tuberculosis complex (MTC) using the viable bacteria selection kit for PCR (Gram Positive) (TaKaRa Bio, Inc., Japan), an Ethidium monoazide (EMA) treatment kit was examined. The range of bacterial concentrations in which the detection of dead bacteria was determined as 1.5 × 107-3.0 × 107 CFU/mL, which equivalent to McFarland No. 0.05-0.10. There was a significant difference between results of viable and dead bacteria, and the sensitivity and specificity of this method for culture-negative samples from patients were 83% and...
Source: Japanese Journal of Infectious Diseases - September 28, 2018 Category: Infectious Diseases Authors: Fukuzawa S, Shiho H, Fujita T Tags: Jpn J Infect Dis Source Type: research

Prevalence of Salmonella enterica subspecies enterica in red-eared sliders Trachemys scripta elegans retailed in pet shops in Japan.
Abstract We investigated the prevalence of Salmonella in 227 small red-eared sliders (Trachemys scripta elegans) from 2006 to 2008. One hundred thirty turtles (57.3%) were positive for S. enterica subsp. enterica. Twenty-two serotypes including S. Montevideo, S. Newport, S. Pomona, S. Braenderup, S. Sandiego, and S. Litchfield were identified. Salmonella strains with closely related PFGE patterns were isolated from several shops located in different areas over 2006 to 2008. Antimicrobial resistance was detected among strains of S. Montevideo, S. Newport, S. Braenderup, S. Sandiego, and S. Litchfield. The relatedne...
Source: Japanese Journal of Infectious Diseases - September 28, 2018 Category: Infectious Diseases Authors: Kuroki T, Ishihara T, Nakajima N, Furukawa I, Une Y Tags: Jpn J Infect Dis Source Type: research

HIV/AIDS in Japan: route and age of infection that shaped the epidemics in 1987- 2016.
Abstract In Japan, HIV infection is classified as "HIV" or "AIDS" depending upon whether the infection was detected before or after development of AIDS. For male homosexuals, in the plot of the number of "AIDS" versus the number of "HIV", the plots fell on a straight line with a slope close to 1. When the number of "HIV" stopped increasing, that of "AIDS" stopped increasing. The number of "HIV" in one region or in one age group was correlated with that of other regions or that of other age groups, respectively. No such correlation was detected f...
Source: Japanese Journal of Infectious Diseases - September 28, 2018 Category: Infectious Diseases Authors: Yoshikura H Tags: Jpn J Infect Dis Source Type: research