Rationalization design, soluble expression and PEG modification of highly active recombinant human-porcine uricase mutant protein
Int J Biol Macromol. 2024 Apr 30:131989. doi: 10.1016/j.ijbiomac.2024.131989. Online ahead of print.ABSTRACTUric acid is the end product of purine metabolism in humans due to inactivation of the uricase determined by the mutated uricase gene. Uricase catalyzes the conversion of uric acid into water-soluble allantoin that is easily excreted by the kidneys. Hyperuricemia occurs when the serum concentration of uric acid exceeds its solubility (7 mg/dl). However, modifications to improve the uricase activity is under development for treating the hyperuricemia. Here we designed 7 types of human-porcine chimeric uricase by multi...
Source: International Journal of Biological Macromolecules - May 2, 2024 Category: Biochemistry Authors: Mingjie Tong Shengli Wang Junxi Luan Qiuling Xie Luquan Wang Xiaoyang Shen Sheng Xiong Source Type: research
Burton's line and scar hyperpigmentation in a patient with severe lead poisoning and saturnine gout
(Source: Arthritis and Rheumatology)
Source: Arthritis and Rheumatology - May 2, 2024 Category: Rheumatology Authors: Gisela Garcia ‐Arellano,
Maria Fernanda Ortiz‐Nuño,
Griselda Serna‐Peña,
Juan Pablo Toledo‐Motta,
Ana Victoria Vasquez‐Gallegos,
Dionicio Angel Galarza‐Delgado,
Jesus Alberto Cardenas‐de la Garza Tags: Clinical Image Source Type: research
Nurses ’ roles, interventions, and implications for management of rheumatic diseases
This article provides a comprehensive review of nurses ’ roles, interventions, and impacts in several rheumatic diseases, including rheumatoid arthritis, osteoarthritis, spondyloarthritis, gout, systemic lupus erythematosus, and polymyalgia rheumatica. It has been demonstrated that care under nursing supervision is effective and safe, with benefits in cluding disease management, quality of life, and treatment adherence. In addition, nurses play a crucial role in promoting health, educating patients, and administering biological disease-modifying anti-rheumatic drugs. The COVID-19 pandemic has highlighted the significance...
Source: Rheumatology International - May 2, 2024 Category: Rheumatology Source Type: research
Recurrent Gout and Serum Urate
To the Editor A recent study found that serum urate levels were associated with the risk of subsequent gout flares and hospitalizations for recurrent gout. However, urate-lowering therapy did not significantly alter the association between serum urate levels and the risk of acute gout flares. These findings diverge from management guidelines recommending urate-lowering therapy and maintenance of serum urate levels less than 6 mg/dL to prevent recurrent gout flares. We are concerned that the diverse treatment adherence rates in this study might have influenced the outcomes of recurrent gout flares. At baseline, 43% of the g...
Source: JAMA - Journal of the American Medical Association - May 2, 2024 Category: General Medicine Source Type: research
Recurrent Gout and Serum Urate
To the Editor A recent Review supported use of baseline serum urate level to assess the risk of recurrent gout. A concern raised by the accompanying Editorial pertained to the reliability of a single serum urate measurement. (Source: JAMA - Journal of the American Medical Association)
Source: JAMA - Journal of the American Medical Association - May 2, 2024 Category: General Medicine Source Type: research
Recurrent Gout and Serum Urate —Reply
In Reply Our recent article quantified the role of serum urate, the central causal biomarker for gout, and found graded associations with recurrent gout flares, including those resulting in hospitalizations (with 100% of these cases associated with serum urate ≥5 mg/dL). Regarding the comments from Drs Chang and Hung, our study did not directly evaluate whether urate-lowering therapy affects gout flares, but rather one of our subgroup analyses tested whether the degree of association of serum urate with flares differed between those treated with urate-l owering therapy vs those not treated with urate-lowering therapy and...
Source: JAMA - Journal of the American Medical Association - May 2, 2024 Category: General Medicine Source Type: research
Nurses ’ roles, interventions, and implications for management of rheumatic diseases
This article provides a comprehensive review of nurses ’ roles, interventions, and impacts in several rheumatic diseases, including rheumatoid arthritis, osteoarthritis, spondyloarthritis, gout, systemic lupus erythematosus, and polymyalgia rheumatica. It has been demonstrated that care under nursing supervision is effective and safe, with benefits in cluding disease management, quality of life, and treatment adherence. In addition, nurses play a crucial role in promoting health, educating patients, and administering biological disease-modifying anti-rheumatic drugs. The COVID-19 pandemic has highlighted the significance...
Source: Rheumatology International - May 2, 2024 Category: Rheumatology Source Type: research
SGLT-2 inhibitors May Decrease Recurrent Gout Flares in Patients with Type 2 diabetes
Dr. Bernal
Clinical question: Do SGLT-2 inhibitors decrease gout flares in patients with type 2 diabetes and a known history of gout?
Background: Sodium–glucose cotransporter-2 inhibitors (SGLT-2 inhibitors) are known to decrease serum urate levels and the risk of incident gout. However, their role in secondary prevention of gout flares is unknown.
Study design: Propensity score–matched, new-user cohort study
Setting: British Columbia, Canada
Synopsis: Using a group of population-based, linked, administrative databases, the population of British Columbia was studied. 15,067 patients with gout and type 2 diabetes had f...
Source: The Hospitalist - May 1, 2024 Category: Hospital Management Authors: Ronda Whitaker Tags: Autoimmune Diseases In the Literature Pharmacology Rheumatology Source Type: research
Design, synthesis, and biological evaluation of 5-(1H-indol-5-yl)isoxazole-3-carboxylic acids as novel xanthine oxidase inhibitors
Eur J Med Chem. 2024 Apr 26;271:116443. doi: 10.1016/j.ejmech.2024.116443. Online ahead of print.ABSTRACTXanthine oxidase (XO) is a key enzyme for the production of uric acid in the human body. XO inhibitors (XOIs) are clinically used for the treatment of hyperuricemia and gout, as they can effectively inhibit the production of uric acid. Previous studies indicated that both indole and isoxazole derivatives have good inhibitory effects against XO. Here, we designed and synthesized a novel series of N-5-(1H-indol-5-yl)isoxazole-3-carboxylic acids according to bioisosteric replacement and hybridization strategies. Among the ...
Source: European Journal of Medicinal Chemistry - May 1, 2024 Category: Chemistry Authors: Dongqian Huang Wenye Li Yilan Zhao Cheng Xie Xiaogang Luo Fengshou Wu Zhiqiang Xu Qi Sun Genyan Liu Source Type: research
Design, synthesis, and biological evaluation of 5-(1H-indol-5-yl)isoxazole-3-carboxylic acids as novel xanthine oxidase inhibitors
Eur J Med Chem. 2024 Apr 26;271:116443. doi: 10.1016/j.ejmech.2024.116443. Online ahead of print.ABSTRACTXanthine oxidase (XO) is a key enzyme for the production of uric acid in the human body. XO inhibitors (XOIs) are clinically used for the treatment of hyperuricemia and gout, as they can effectively inhibit the production of uric acid. Previous studies indicated that both indole and isoxazole derivatives have good inhibitory effects against XO. Here, we designed and synthesized a novel series of N-5-(1H-indol-5-yl)isoxazole-3-carboxylic acids according to bioisosteric replacement and hybridization strategies. Among the ...
Source: European Journal of Medicinal Chemistry - May 1, 2024 Category: Chemistry Authors: Dongqian Huang Wenye Li Yilan Zhao Cheng Xie Xiaogang Luo Fengshou Wu Zhiqiang Xu Qi Sun Genyan Liu Source Type: research
Clustering of gout-related comorbidities and their relationship with gout flares: a data-driven cluster analysis of eight comorbidities
ConclusionsWe clustered people with gout into five groups with varying comorbidities. People with CVD, CKD, and cancer had the highest risk of gout flares and should receive comprehensive care. (Source: Journal of Endocrinological Investigation)
Source: Journal of Endocrinological Investigation - May 1, 2024 Category: Endocrinology Source Type: research
Predictors of Inadequate Serum Urate Response to Low-Dose Febuxostat in Male Patients with Gout
(Source: Journal of Inflammation Research)
Source: Journal of Inflammation Research - April 30, 2024 Category: Allergy & Immunology Tags: Journal of Inflammation Research Source Type: research
Prediction and causal inference of hyperuricemia using gut microbiota
This study aimed to determine the relationship between HUA and GM. The microbiome of 224 men and 254 women aged 40 years was analyzed through next-generation sequencing and machine learning. We obtained GM data through 16S rRNA-based sequencing of the fecal samples, finding that alpha-diversity by Shannon index was significantly low in the HUA group. Linear discriminant effect size analysis detected a high abundance of the genera Collinsella and Faecalibacterium in the HUA and non-HUA groups. Based on light gradient boosting machine learning, we propose that HUA can be predicted with high AUC using four clinical characteri...
Source: Atherosclerosis - April 30, 2024 Category: Cardiology Authors: Yuna Miyajima Shigehiro Karashima Ren Mizoguchi Masaki Kawakami Kohei Ogura Kazuhiro Ogai Aoi Koshida Yasuo Ikagawa Yuta Ami Qiunan Zhu Hiromasa Tsujiguchi Akinori Hara Shin Kurihara Hiroshi Arakawa Hiroyuki Nakamura Ikumi Tamai Hidetaka Nambo Shigefumi O Source Type: research
The association between Helicobacter pylori infection and the risk for gout in hyperuricemia patients in China – A cross-sectional study
ConclusionH. pylori infection is positively associated with higher risk of gout in hyperuricemia population. The causal relationship and potential mechanism betweenH. pylori infection and gout warrants further investigation. (Source: Gut Pathogens)
Source: Gut Pathogens - April 27, 2024 Category: Microbiology Source Type: research
Evaluation of the inhibitory effects of antigout drugs on human carboxylesterases in vitro
Toxicol In Vitro. 2024 Apr 24:105833. doi: 10.1016/j.tiv.2024.105833. Online ahead of print.ABSTRACTGout is an immune-metabolic disease that frequently coexists with multiple comorbidities such as chronic kidney disease, cardiovascular disease and metabolic syndrome, therefore, it is often treated in combination with these complications. The present study aimed to evaluate the inhibitory effect of antigout drugs (allopurinol, febuxostat, topiroxostat, benzbromarone, lesinurad and probenecid) on the activity of the crucial phase I drug-metabolizing enzymes, carboxylesterases (CESs). 2-(2-benzoyl-3-methoxyphenyl) benzothiazo...
Source: Toxicology in Vitro - April 26, 2024 Category: Toxicology Authors: Jia-Hong Liang Xiao-Lei Yi Jia-Min Gong Zuo Du Source Type: research
