A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites
ConclusionsThe assay is a convenient and cost-effective method that could contribute to a quicker and accurate diagnosis as well as to more targeted therapies of parasite-derived diarrhea. Finally, this new multiplex PCR assay could also be instrumental in epidemiology studies on these parasites. (Source: Gut Pathogens)
Source: Gut Pathogens - May 29, 2020 Category: Microbiology Source Type: research

Clostridioides difficile ribotype distribution in a large teaching hospital in Serbia
AbstractBackgroundThe global epidemic of nosocomial diarrhea caused byClostridioides (Clostridium) difficile started in 2000, with high mortality rates and emergence of a new hypervirulent strain NAP1/BI/027. The aim of this study was to assess the presence of ribotype 027 and otherC. difficile ribotypes in a Serbian University Hospital, compare the temporal variability of ribotypes 3  years apart, as well as to compare clinical, demographic and laboratory characteristics and disease outcome among patients infected with 027 and non-027 ribotype. This was a prospective observational cohort study addressing 4-month inte...
Source: Gut Pathogens - May 22, 2020 Category: Microbiology Source Type: research

Type IV secretion of Helicobacter pylori CagA into oral epithelial cells is prevented by the absence of CEACAM receptor expression
ConclusionsThese results show that lack of CEACAM receptors on the surface of the oral epithelial cells was responsible for resistance toH. pylori CagA-dependent pathogenic activities, and confirms the important role for the T4SS-dependent interaction of these receptors withH. pylori in the gastric epithelium. (Source: Gut Pathogens)
Source: Gut Pathogens - May 14, 2020 Category: Microbiology Source Type: research

Host responses to Clostridium perfringens challenge in a chicken model of chronic stress
ConclusionCORT administration modulated a number of host functions, which corresponded to increased densities ofC. perfringens in the small intestine and weight gain impairment in chickens. Importantly, results implicate physiological stress as an important predisposing factor to NE, which emphasizes the importance of managing stress to optimize chicken health. (Source: Gut Pathogens)
Source: Gut Pathogens - May 6, 2020 Category: Microbiology Source Type: research

Exosomal miRNAs in hepatitis B virus related liver disease: a new hope for biomarker
AbstractThe World Health Organisation, in its 2019 progress report on HIV, viral hepatitis and STDs indicates that 257 million people are afflicted with chronic HBV infections, of which, 1 million patients lose their lives every year due to HBV related chronic liver diseases including serious complications such as liver cirrhosis and hepatocellular carcinoma. The course of HBV infection and associated liver injury depend on several host factors, genetic variability of the virus, and the host viral interplay. The challenge of medical science is the early diagnosis/identification of the potential for development of fatal com...
Source: Gut Pathogens - April 24, 2020 Category: Microbiology Source Type: research

EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cells
ConclusionsEV71 infection can trigger ROS production, cell death, and inflammatory reactions by modulating the Nrf2 and Keap1 levels of infected cells. (Source: Gut Pathogens)
Source: Gut Pathogens - April 23, 2020 Category: Microbiology Source Type: research

Prevalence and antimicrobial susceptibility of Arcobacter species in human stool samples derived from out- and inpatients: the prospective German Arcobacter prevalence study Arcopath
ConclusionsIn summary,Arcobacter includingA. butzleri,A. cryaerophilus andA. lanthieri could be isolated in 0.85% of German outpatients and ciprofloxacin rather than other antibiotics might be appropriate for antibiotic treatment of infections. Further epidemiological studies are needed, however, to provide a sufficient risk assessment ofArcobacter infections in humans. (Source: Gut Pathogens)
Source: Gut Pathogens - April 15, 2020 Category: Microbiology Source Type: research

Antibacterial activity of ethoxzolamide against Helicobacter pylori strains SS1 and 26695
AbstractWith the rise of bacterial resistance to conventional antibiotics, re-purposing of Food and Drug Administration (FDA) approved drugs currently used to treat non-bacteria related diseases as new leads for antibacterial drug discovery has become an attractive alternative. Ethoxzolamide (EZA), an FDA-approved diuretic acting as a human carbonic anhydrase inhibitor, is known to kill the gastric pathogenic bacteriumHelicobacter pylori in vitro via an, as yet, unknown mechanism. To date, EZA activity and resistance have been investigated for only oneH. pylori strain, P12. We have now performed a susceptibility and resist...
Source: Gut Pathogens - April 15, 2020 Category: Microbiology Source Type: research

Molecular epidemiology of rotavirus A and adenovirus among children with acute diarrhea in Hangzhou, China
ConclusionsG9P[8] of RVA and Adv-41 were the most common genotypes that caused children ’s acute diarrhea in Hangzhou, 2017–2018. (Source: Gut Pathogens)
Source: Gut Pathogens - April 13, 2020 Category: Microbiology Source Type: research

Distribution of genes related to Type 6 secretion system and lipooligosaccharide that induced ganglioside mimicry among Campylobacter jejuni isolated from human diarrhea in Thailand
AbstractBackgroundCampylobacter jejuni (C. jejuni) is one of the most common bacteria responsible for human gastroenteritis worldwide. The mode of human transmission is foodborne infections due to consumption of contaminated food, especially poultry. Type 6 secretion systems (T6SS) were described recently asCampylobacter virulence mechanisms. Furthermore, infection sequelae associated with neurological disorders like Guillain –Barré (GBS) and Miller Fisher (MF) syndromes can become serious health problems in some patients afterCampylobacter gastroenteritis. Our objective was to determine the distribution of th...
Source: Gut Pathogens - April 9, 2020 Category: Microbiology Source Type: research

Enteric etiological surveillance in acute diarrhea stool of United States Military Personnel on deployment in Thailand, 2013 –2017
This study describes the prevalence of acute diarrheal illness etiological agents, their antibiotic resistance distribution patterns, the resulting impact upon military force health protection, and potential prevention and treatment strategies.ResultsForty-eight acute diarrhea stool samples from US military personnel deployed to Thailand from 2013 –2017 were screened for enteric pathogens using ELISA, the TaqMan Array Card (TAC), and conventional microbiological methods. These isolates were also evaluated using antimicrobial susceptibility testing (AST) against ampicillin (AMP), azithromycin (AZM), ceftriaxone (CRO),...
Source: Gut Pathogens - April 9, 2020 Category: Microbiology Source Type: research

Profound remission in Crohn ’s disease requiring no further treatment for 3–23 years: a case series
ConclusionsProlonged remission has been achieved for 3 –23 years with individualised treatments, with the majority using AMAT ± infliximab and FMT. Treatment with antibiotics and/or FMT provides a potential new avenue for treatment of CD. These findings should stimulate thinking, investigations and better therapy against MAP and the dysbiosis o f the gut flora, to enable higher rates of prolonged remission. (Source: Gut Pathogens)
Source: Gut Pathogens - April 9, 2020 Category: Microbiology Source Type: research

Proof-of-concept trial of the combination of lactitol with Bifidobacterium bifidum and Lactobacillus acidophilus for the eradication of intestinal OXA-48-producing Enterobacteriaceae
ConclusionsThe combination of prebiotics and probiotics was well tolerated. A rapid reduction on the OXA-PE intestinal loads was observed. At the EoT, decolonization was achieved in three patients.Clinical Trials Registration: NCT02307383. EudraCT Number: 2014-000449-65. (Source: Gut Pathogens)
Source: Gut Pathogens - April 7, 2020 Category: Microbiology Source Type: research

Association of antimicrobial resistance and gut microbiota composition in human and non-human primates at an urban ecotourism site
ConclusionHigher antibiotic susceptibility in NHPs is likely related to low direct exposure to antibiotics. The lack of resistance may also suggest limited antimicrobial resistance transmission between humans and NHP. Nonetheless, continued monitoring over a long period will help mitigate the risk of anthropozoonosis and zooanthroponosis. (Source: Gut Pathogens)
Source: Gut Pathogens - March 10, 2020 Category: Microbiology Source Type: research

Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity
In conclusion, this study provides a molecular basis for the non-saccharolytic and hydrogen-dependent nature ofC. concisus energy metabolism pathways, which provides insights into the growth requirements and pathogenicity of this species. (Source: Gut Pathogens)
Source: Gut Pathogens - March 5, 2020 Category: Microbiology Source Type: research

Complete genome of Enterobacter sichuanensis strain SGAir0282 isolated from air in Singapore
ConclusionBased on whole genome alignment and phylogenetic analysis, the strain SGAir0282 was identified to beEnterobacter sichuanensis. The strain possesses gene clusters for virulence, disease and defence, that can also be found in other multidrug resistant ECC type strains. (Source: Gut Pathogens)
Source: Gut Pathogens - February 27, 2020 Category: Microbiology Source Type: research

Gut microbiome of native Arab Kuwaitis
ConclusionsBacteroides was the most abundant genus in the gut microbiome of native Arab Kuwaiti adults, withBacteroides dorei/vulgatus forming the predominant phylogenetic group. The microbiome composition would also have been influenced by the nutritional status of participants. (Source: Gut Pathogens)
Source: Gut Pathogens - February 26, 2020 Category: Microbiology Source Type: research

Effects of co-infection on the clinical outcomes of Clostridium difficile infection
ConclusionOur study suggests that co-infection doesn ’t affect the severity of CDI or can cause treatment failures. Additionally, there was no significant increase in hospital stay, or increase in CDI recurrence associated with co-infection. Therefore, if CDI is the leading clinical diagnosis and a patient is tested positive for co-infection in addi tion to CDI on FilmArray® multiplex GI panel, this co-infection shouldn’t change the management for CDI. Limitations of this study (including retrospective nature of the study, small sample size, single site study, not including all microbiome and non-inclusion ...
Source: Gut Pathogens - February 25, 2020 Category: Microbiology Source Type: research

Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
ConclusionWe conclude that genes regulated by Cj1507c have an impact on efficient adhesion, invasion and intracellular survival ofC. jejuni in HT-29/B6 cells. Furthermore, potential signal sensing by Cj1492c seems to lead to limiting attachment and hence internalisation ofC. jejuni. However, as the intracellular survival capacities are reduced, we suggest that signal sensing by Cj1492c impacts several processes related to pathogenicity ofC. jejuni. (Source: Gut Pathogens)
Source: Gut Pathogens - February 11, 2020 Category: Microbiology Source Type: research

Gut microbiota changes in children with autism spectrum disorder: a systematic review
ConclusionsThese results were inadequate to confirm a global microbiome change in children with ASD and causality could not be inferred to explain the etiology of the behaviors associated with ASD. Mechanistic studies are needed to elucidate the specific role of the gut microbiome in the pathogenesis of ASD. (Source: Gut Pathogens)
Source: Gut Pathogens - February 3, 2020 Category: Microbiology Source Type: research

Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh
ConclusionWidespread dissemination ofE. coli strains carryingmcr-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly. (Source: Gut Pathogens)
Source: Gut Pathogens - January 27, 2020 Category: Microbiology Source Type: research

Phenotypic zinc resistance does not correlate with antimicrobial multi-resistance in fecal E. coli isolates of piglets
ConclusionOur findings argue against a co-selection mechanism of antimicrobial drug-resistance and zinc tolerance after dietary zinc supplementation in weaning piglets. An explanation for an increase in multi-drug resistant isolates from piglets with high zinc dietary feeding could be that resistant bacteria to antimicrobial agents are more persistent to stresses such as zinc or copper exposure. (Source: Gut Pathogens)
Source: Gut Pathogens - January 21, 2020 Category: Microbiology Source Type: research

Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model
ConclusionThe loweredC. jejuni loads and alleviated symptoms observed in the here applied clinical murine model for human campylobacteriosis highlight the application of carvacrol as a promising novel option for both, the treatment of campylobacteriosis and hence, for prevention of post-infectious sequelae in humans, and for the reduction ofC. jejuni colonization in the intestines of vertebrate lifestock animals. (Source: Gut Pathogens)
Source: Gut Pathogens - January 8, 2020 Category: Microbiology Source Type: research

Characterization of Arcobacter strains isolated from human stool samples: results from the prospective German prevalence study Arcopath
ConclusionsOur study provides evidence for the abundance of putative virulence genes inArcobacter isolates and prominent cytotoxic effects ofA. butzleri andA. lanthieriin vitro. The presence ofcdtA,cdtB,cdtC inA. lanthieri points towards CDT secretion as potential mechanism underlying cytotoxicity as opposed toA. butzleri. However, the association of theArcobacter virulence factors detected and human morbidity should be addressed in future studies. (Source: Gut Pathogens)
Source: Gut Pathogens - January 8, 2020 Category: Microbiology Source Type: research

Microbial imbalance in inflammatory bowel disease patients at different taxonomic levels
ConclusionsIBD patients exhibited a less diverse gut microbiome compared to healthy individuals. Bacterial groups which changed in IBD patients were found to be groups which do not co-exist well with common commensal gut bacteria, whereas bacterial groups which did not change in patients with IBD were found to commonly co-exist with commensal gut microbiota. This gives a potential insight into the dynamics of the gut microbiota in patients with IBD. (Source: Gut Pathogens)
Source: Gut Pathogens - January 4, 2020 Category: Microbiology Source Type: research

Comparison of different technologies for the decipherment of the whole genome sequence of Campylobacter jejuni BfR-CA-14430
ConclusionThe comparison of Illumina MiSeq, PacBio and MinION sequencing and analyses with different assembly tools enabled us to reconstruct a complete chromosome as well as a circular plasmid sequence of theC. jejuni strain BfR-CA-14430. Illumina short-read sequencing in combination with either PacBio or MinION can substantially improve the quality of the complete chromosome and epichromosomal elements on the level of mismatches and insertions/deletions, depending on the assembly program used. (Source: Gut Pathogens)
Source: Gut Pathogens - December 16, 2019 Category: Microbiology Source Type: research

The intestinal microbial community dissimilarity in hepatitis B virus-related liver cirrhosis patients with and without at alcohol consumption
ConclusionsOur study demonstrated that elevatedFirmicutes/Bacteroidetes ratio, reducedMegamonas genus level and increasedVeillonella genus level were indicators for HBV-related liver cirrhosis. Alcohol-related pathogenesis was associated with the changed microbial community. (Source: Gut Pathogens)
Source: Gut Pathogens - November 26, 2019 Category: Microbiology Source Type: research

Correction to: Involvement of gut microbiome in human health and disease: brief overview, knowledge gaps and research opportunities
Following publication of the original article in Gut Pathogens [1], it was brought to our attention that Dr. Ross Ka Kit Leung ’s affiliation was incomplete. Dr. Leung is appointed as an Adjunct Professor of Guangzhou Medical University from January 1st 2017 to December 31st 2019. He would like to correct here the affiliation and acknowledge his role at the Guangzhou Medical University when this article was published. (Source: Gut Pathogens)
Source: Gut Pathogens - November 21, 2019 Category: Microbiology Source Type: research

Investigation of molecular mechanisms underlying tetracycline resistance in thermophilic Campylobacter spp. suggests that previous reports of tet (A)-mediated resistance in these bacteria are premature
In conclusion, further work would be required to determine whether the homology betweentet(A) potentially present inCampylobacter and knowntet(A) genes would be sufficient to allow amplification using the primers designed in our study. Finally, the existence oftet(A) in thermophilicCampylobacter spp. remains to be demonstrated. (Source: Gut Pathogens)
Source: Gut Pathogens - November 9, 2019 Category: Microbiology Source Type: research

Complete genome analysis of clinical Shigella strains reveals plasmid pSS1653 with resistance determinants: a triumph of hybrid approach
AbstractShigella is ranked as the second leading cause of diarrheal disease worldwide. Though infection occurs in people of all ages, most of the disease burden constitutes among the children less than 5  years in low and middle income countries. Recent increasing incidence of drug resistant strains make this as a priority pathogen under the antimicrobial resistance surveillance by WHO. Despite this, only limited genomic studies on drug resistantShigella exists. Here we report the first complete genome of clinicalS. flexneri serotype 2a andS. sonnei strains using a hybrid approach of both long-read MinION (Oxford Nano...
Source: Gut Pathogens - November 6, 2019 Category: Microbiology Source Type: research

Hypervirulent clade 2, ribotype 019/sequence type 67 Clostridioides difficile strain from Japan
ConclusionOur findings contribute to the further understanding of the characteristics of hypervirulent clade 2 including RT019/ST67 lineages. (Source: Gut Pathogens)
Source: Gut Pathogens - November 4, 2019 Category: Microbiology Source Type: research

Occurrence of Escherichia coli and faecal coliforms in drinking water at source and household point-of-use in Rohingya camps, Bangladesh
ConclusionsFrom our findings, it appears that secondary contamination could be a function of very high population density and could possibly occur during collection, transportation, and storage of water due to lack of knowledge of personal and domestic hygiene. Hence, awareness campaign is necessary, and the contaminated sources should be replaced. Further, the POU water should be treated by a suitable method. (Source: Gut Pathogens)
Source: Gut Pathogens - November 1, 2019 Category: Microbiology Source Type: research

RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagic Escherichia coli O157:H7
ConclusionsIn summary, the RstAB TCS in EHEC O157 plays a major role in the regulation of virulence, acid tolerance, and biofilm formation. We clarified the regulatory function of RstA, providing an insight into mechanisms that may be potential drug targets for treatment of EHEC O157-related infections. (Source: Gut Pathogens)
Source: Gut Pathogens - November 1, 2019 Category: Microbiology Source Type: research

Evaluation of the Vibrant DNA microarray for the high-throughput multiplex detection of enteric pathogens in clinical samples
ConclusionThe Vibrant GPP is a comprehensive, high-throughput, and rapid DNA microarray to provide etiologic diagnosis of GI infections in the laboratory setting. (Source: Gut Pathogens)
Source: Gut Pathogens - October 18, 2019 Category: Microbiology Source Type: research

Metagenomics: aid to combat antimicrobial resistance in diarrhea
AbstractAntimicrobial resistance (AMR) has emerged as an obstacle in the supple administration of antimicrobial agents to critical diarrheal patients. Most diarrheal pathogens have developed resistance against the major classes of antibiotics commonly used for assuaging diarrheal symptoms. Antimicrobial resistance develops when pathogens acquire antimicrobial resistance genes (ARGs) through genetic recombination from commensals and pathogens. These are the constituents of the complex microbiota in all ecological niches. The recombination events may occur in the environment or in the gut. Containment of AMR can be achieved ...
Source: Gut Pathogens - October 14, 2019 Category: Microbiology Source Type: research

Is there any association between gut microbiota and type 1 diabetes? A systematic review
ConclusionOur study showed a significant association between alterations in intestinal microbial composition and T1D; however, in some articles, it is not clear which one happens first. Investigation of altered gut microbiota can help in the early detection of T1D before seropositivity. Targeted microbiome modulation can be a novel potential therapeutic strategy. (Source: Gut Pathogens)
Source: Gut Pathogens - October 14, 2019 Category: Microbiology Source Type: research

First complete genome sequence and comparative analysis of Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) indicates host adaptation traits to sheep
ConclusionsWith this sequence, we provide the first complete and closed genome sequence of a SASd strain. With this study, we provide an important basis for an understanding of the genetic mechanism that underlie pathogenicity and host adaptation of SASd to sheep. (Source: Gut Pathogens)
Source: Gut Pathogens - October 14, 2019 Category: Microbiology Source Type: research

Correction to: The pathogenic intestinal spirochaete Brachyspira pilosicoli forms a diverse recombinant species demonstrating some local clustering of related strains and potential for zoonotic spread
The article published in 2013 [1] described the use of the availableBrachyspira multilocus sequence typing (MLST) scheme [2] to characterize the population structure of the intestinal spirocheteBrachyspira pilosicoli. It used sequences of seven loci that were amplified from 131 strains that had been isolated from different geographical origins and species. (Source: Gut Pathogens)
Source: Gut Pathogens - October 14, 2019 Category: Microbiology Source Type: research

Shiga toxin producing Escherichia coli -associated diarrhea and hemolytic uremic syndrome in young children in Romania
ConclusionsSTEC prevalence among young children with diarrhea in Romania was high and the risk of HUS is emerging. The establishment of a systematic laboratory-based surveillance program including identification of the circulating STEC strains coupled with epidemiological investigation of HUS patients is warranted to determine the source and mode of transmission of STEC and prevent of STEC-associated diarrhea and HUS. (Source: Gut Pathogens)
Source: Gut Pathogens - September 25, 2019 Category: Microbiology Source Type: research

Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils
ConclusionsWe confirmed mutations in genes previously reported to be associated with adaptation to Mongolian gerbils. We also listed up genes that mutated during the infection to the gerbils, though it needs experiments to prove the influence on adaptation. (Source: Gut Pathogens)
Source: Gut Pathogens - September 21, 2019 Category: Microbiology Source Type: research

First bloodstream infection caused by Prevotella copri in a heart failure elderly patient with Prevotella -dominated gut microbiota: a case report
ConclusionsWe provide the first evidence for a clinically significant BSI caused byP. copri and its relationship to aPrevotella-rich gut microbiota in the HF patient setting. When strengthening the pathogenicity ofP. copri, the present case suggests that the gut may be a source of BSI caused by the rare anaerobic organism. Future studies are necessary to assess the role of the gut microbiota profiling for precise identification and targeted treatment of patients at high risk of BSI. (Source: Gut Pathogens)
Source: Gut Pathogens - September 18, 2019 Category: Microbiology Source Type: research

Preliminary feasibility for recruiting and retaining black and white females to provide fecal samples for longitudinal research
This article provides preliminary evidence of the feasibility of recruiting and retaining a racially diverse sample of females (97% completion for invited participants) for longitudinal microbiome research. (Source: Gut Pathogens)
Source: Gut Pathogens - August 26, 2019 Category: Microbiology Source Type: research

Differences in virulence gene expression between human blood and stool Campylobacter coli clade 1 ST828CC isolates
ConclusionsThis study reveals interesting differences betweenC. coli clade 1 isolates of human and animal origin on one hand, and also between human blood and stool isolates, on the other. The results suggest thatC. coli might downregulate and/or inactivate various virulence determinants as the isolates pass from the animal host to the human gastrointestinal tract and enter the human blood stream. (Source: Gut Pathogens)
Source: Gut Pathogens - August 1, 2019 Category: Microbiology Source Type: research

Synbiotic-like effect of linoleic acid overproducing Lactobacillus casei with berry phenolic extracts against pathogenesis of enterohemorrhagic Escherichia coli
ConclusionsThese results showed that diet containing bioactive Lc  + CLA and natural prebiotic like component such as BPEs might be an effective way to prevent foodborne infections with EHEC. (Source: Gut Pathogens)
Source: Gut Pathogens - July 27, 2019 Category: Microbiology Source Type: research

Genotypic prevalence of norovirus GII in gastroenteritis outpatients in Shanghai from 2016 to 2018
ConclusionUnlike the alternating circulation of GII.4 and non-GII.4 NoV observed in 2016 or 2017, the genotype profile of NoV GII in 2018 was characterized by the co-prevalence of multiple recombinant genotypes. A recent increase in detection rate in less reported recombinant genotypes such as GII.P12/GII.3 and GII.P7/GII.6 among adult population calls for a continuing close monitoring on NoV GII genotypes in case of potential local outbreaks. (Source: Gut Pathogens)
Source: Gut Pathogens - July 26, 2019 Category: Microbiology Source Type: research

Differential transcriptome analysis of the disease tolerant Madagascar –Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction
ConclusionsThe outcomes of this research correlate the different stages ofP. monodon immune response to different time points of AHPND infection. This finding supports the development of biomarkers for the detection of early stages ofVpAHPND colonization inP. monodon through host immune expression changes. The potential genes to be utilized as biomarkers include but not limited to C-type lectin, HMGB1, IMD, ALF, serine proteinase, and DSCAM. (Source: Gut Pathogens)
Source: Gut Pathogens - July 26, 2019 Category: Microbiology Source Type: research

Nonhemolysis of epidemic El Tor biotype strains of Vibrio cholerae is related to multiple functional deficiencies of hemolysin A
ConclusionsMechanisms responsible for nonhemolysis of the epidemic O1 El Tor strains are complex and not only confined to gene mutation but also deficiencies of transcription and extracellular transport of HlyA. Mutations in gene regulation and protein secretion systems of HlyA in the nonhemolyticV. cholerae strains should be areas of concern in future studies. (Source: Gut Pathogens)
Source: Gut Pathogens - July 12, 2019 Category: Microbiology Source Type: research

Whole-genome analysis of rotavirus G4P[6] strains isolated from Korean neonates: association of Korean neonates and rotavirus P[6] genotypes
ConclusionsThis study showed that the whole-genome constellation of rotavirus G4P[6] strains from Korean neonates resembled a Wa-like genotype constellation. Additionally, rotavirus genotypes detected in Korean neonates had unique P[6] sequences, which may be the cause of Korean neonatal rotavirus infection. (Source: Gut Pathogens)
Source: Gut Pathogens - July 10, 2019 Category: Microbiology Source Type: research

Recombinant fusion protein of Heparin-Binding Hemagglutinin Adhesin and Fibronectin Attachment Protein (rHBHA-FAP) of Mycobacterium avium subsp. paratuberculosis elicits a strong gamma interferon response in peripheral blood mononuclear cell culture
ConclusionsCollectively, these results demonstrate that rHBHA-FAP elicits a strong IFN- γ production in PBMC culture. Therefore, further studies of the present product as a candidate vaccine in naturally infected animals should be conducted, to analyze its potential. (Source: Gut Pathogens)
Source: Gut Pathogens - July 9, 2019 Category: Microbiology Source Type: research

Validating the inhibitory effects of d - and l -serine on the enzyme activity of d -3-phosphoglycerate dehydrogenases that are purified from Pseudomonas aeruginosa , Escherichia coli and human colon
ConclusionsThese results suggest thatl-serine significantly repressedP. aeruginosa pathogenicity through direct inhibition of the PGDH activity, but was not able to inhibit the human PGDH activity. Oral administration ofl-serine to compromised hosts might interfere with bacterial translocation and prevent gut-derived sepsis caused byP. aeruginosa through inhibition of the function of theserA gene product. (Source: Gut Pathogens)
Source: Gut Pathogens - June 30, 2019 Category: Microbiology Source Type: research