Intestinal parasites among intellectually disabled individuals in Iran: a systematic review and meta-analysis
ConclusionIPIs are highly prevalent among intellectually disabled individuals in Iran. Improving the health status and implementing infectious disease prevention strategies in rehabilitation centers, health promotion interventions to improve personal hygiene of intellectually disabled individuals, as well as utilize sensitive diagnostic methods besides routine stool examination techniques, and treatment of infected individuals will help in the control of these infections among intellectually disabled individuals. (Source: Gut Pathogens)
Source: Gut Pathogens - May 1, 2021 Category: Microbiology Source Type: research

Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics
ConclusionsThe phylogenetic trees grouped the majority of theS. Typhimurium isolates from humans into a single cluster suggesting that there is one prevalent subtype in Brazil. Regarding strains isolated from food and swine, the SNPs ’ results suggested the circulation of more than one subtype over 30 years in this country. The orthologous protein clusters analysis revealed unique genes in the strains studied mainly related to bacterial metabolism.S. Typhimurium strains from swine showed greater diversity of STs and prophages in comparison to strains isolated from humans and foods. The pathogenic potential ofS. Typhi...
Source: Gut Pathogens - April 28, 2021 Category: Microbiology Source Type: research

Helicobacter cinaedi bacteraemia secondary to enterocolitis in an immunocompetent patient
ConclusionsIn conclusion, this case ofH. cinaedi bacteraemia secondary to enterocolitis in an immunocompetent patient provide clear evidence that one route of infection occurs through translocation from the intestinal tract to the bloodstream.Helicobacter cinaedi from blood and faeces were identical with a novel ST20, resistant to ciprofloxacin and clarithromycin however, the patient was cured with oral pivampicillin. (Source: Gut Pathogens)
Source: Gut Pathogens - April 23, 2021 Category: Microbiology Source Type: research

Association of small intestinal bacterial overgrowth with Parkinson ’s disease: a systematic review and meta-analysis
AbstractObjectiveParkinson ’s disease (PD) is the second most prevalent neurodegenerative disease after Alzheimer's disease (AD) worldwide. The prevalence of small intestinal bacterial overgrowth (SIBO) in PD patients is high. We conducted this comprehensive systematic review and meta-analysis to determine the association b etween SIBO and PD.MethodsA comprehensive literature search of the PubMed, Cochrane Library and EMBASE databases was performed to identify studies correlating SIBO with PD. Studies were screened, and relevant data were extracted and analysed. We calculated the pooled prevalence of SIBO in all indi...
Source: Gut Pathogens - April 16, 2021 Category: Microbiology Source Type: research

Cytomegalovirus ‐associated esophagitis on early esophageal cancer in immunocompetent host: a case report
ConclusionsThis is the first report of a case of CMV esophagitis superimposed on early esophageal cancer in an immunocompetent host and might provide valuable information for possible adverse effects of steroid administration during ESD procedures, despite their common use for prevention of post-ESD stricture. (Source: Gut Pathogens)
Source: Gut Pathogens - April 16, 2021 Category: Microbiology Source Type: research

Diversity of 3 ′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population
ConclusionsIn this study, 503 CagA sequences were studied and analyzed in depth. In Chinese population, mostH. pylori strains were of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer. (Source: Gut Pathogens)
Source: Gut Pathogens - April 13, 2021 Category: Microbiology Source Type: research

Potential capacity of interferon- α to eliminate covalently closed circular DNA (cccDNA) in hepatocytes infected with hepatitis B virus
AbstractInterferon-alpha (IFN- α) and nucleot(s)ide analogs (NAs) are first-line drugs for the treatment of chronic hepatitis B virus (HBV) infections. Generally, NAs target the reverse transcription of HBV pregenomic RNA, but they cannot eliminate covalently-closed-circular DNA (cccDNA). Although effective treatment with NAs ca n dramatically decrease HBV proteins and DNA loads, and even promote serological conversion, cccDNA persists in the nucleus of hepatocytes due to the lack of effective anti-cccDNA drugs. Of the medications currently available, only IFN-α can potentially target cccDNA. However, the clini...
Source: Gut Pathogens - April 12, 2021 Category: Microbiology Source Type: research

Microbiome profiling of rotavirus infected children suffering from acute gastroenteritis
ConclusionsThe observations assert significant microbial signatures of RVV, which is dose-dependent, and suggest manipulating these microbes as a novel approach for improving RVV efficacy. Further studies are warranted to investigate the immune status of these patients and mechanistic investigation to enhance RVV seroconversion. (Source: Gut Pathogens)
Source: Gut Pathogens - March 29, 2021 Category: Microbiology Source Type: research

Phylogenetic analysis of HIV-1 archived DNA in blood and gut-associated lymphoid tissue in two patients under antiretroviral therapy
AbstractOne of the approaches to cure human immunodeficiency virus (HIV) is the use of therapeutic vaccination. We have launched the Provir/Latitude 45 study to identify conserved CTL epitopes in archived HIV-1 DNA according to the HLA class I alleles in aviremic patients under antiretroviral therapy (ART). A HIV-1 polypeptidic therapeutic vaccine based on viral sequence data obtained from circulating blood was proposed; here, our aim was to compare the proviral DNA in blood and gut-associated lymphoid tissue (GALT). Peripheral blood mononuclear cells and gut biopsies were obtained from two HIV-1 infected patients under su...
Source: Gut Pathogens - March 23, 2021 Category: Microbiology Source Type: research

Mucosa ‐associated cultivable aerobic gut bacterial microbiota among colorectal cancer patients attending at the referral hospitals of Amhara Regional State, Ethiopia
ConclusionsThe overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings. (Source: Gut Pathogens)
Source: Gut Pathogens - March 22, 2021 Category: Microbiology Source Type: research

Diarrhea in an infant due to Shigella flexneri 1 carrying multiple cephalosporinase-encoding genes
ConclusionsThis report highlights the emergence of extremely resistantShigella that has acquired multiple resistance genes to cephalosporins rendering these drugs ineffective. (Source: Gut Pathogens)
Source: Gut Pathogens - March 20, 2021 Category: Microbiology Source Type: research

Proton pump inhibitors use and risk of developing spontaneous bacterial peritonitis in cirrhotic patients: A systematic review and meta-analysis
AbstractBackgroundSpontaneous bacterial peritonitis (SBP) is one of the most common infectious diseases in patients with cirrhosis and is associated with serious prognosis. A prevailing dogma posits that SBP is exacerbated by the frequent use of proton pump inhibitors (PPIs).AimsTo re-assess the association between PPIs use and SBP incidence with larger and better-quality data.MethodThe studies were identified by searching Proquest, Medline, and Embase for English language articles published between January 2008 and March 2020 using the following keywords alone or in combination: anti-ulcer agent, antacid, proton pump inhi...
Source: Gut Pathogens - March 19, 2021 Category: Microbiology Source Type: research

No distinction in the gut microbiota between diarrhea predominant-irritable bowel syndrome and healthy subjects: matched case –control study in Thailand
ConclusionAlteration in the gut microbiota composition was probably not the main pathogenic mechanism in the Thai IBS-D patients assessed in this study. However, modifying microbiomes with potentially protective bacteria seems to be a beneficial therapy.Thai Clinical trial registry: TCTR20191211006, Date of registration: 10 Dec 2019. Retrospectively registered, Clinical trial URL:www.clinicaltrials.in.th (Source: Gut Pathogens)
Source: Gut Pathogens - March 3, 2021 Category: Microbiology Source Type: research

Transcriptional regulation of the virulence genes and the biofilm formation associated operons in Vibrio parahaemolyticus
ConclusionQsvR directly repressedtoxR andcalR transcription as well as directly activatedcpsQ-mfpABC andmfpABC transcription. The data presented here promotes us to gain deeper knowledge of the regulatory network of themfp locus inV. parahaemolyticus. (Source: Gut Pathogens)
Source: Gut Pathogens - March 2, 2021 Category: Microbiology Source Type: research

Interplay between severities of COVID-19 and the gut microbiome: implications of bacterial co-infections?
AbstractCOVID-19 is an acute respiratory distress syndrome and is often accompanied by gastrointestinal symptoms. The SARS-CoV-2 has been traced not only in nasopharyngeal and mid-nasal swabs but also in stool and rectal swabs of COVID-19 patients. The gut microbiota is important for an effective immune response as it ensures that unfavorable immune reactions in lungs and other vital organs are regulated. The human gut-lung microbiota interplay provides a framework for therapies in the treatment and management of several pulmonary diseases and infections. Here, we have collated data from COVID-19 studies, which suggest tha...
Source: Gut Pathogens - February 25, 2021 Category: Microbiology Source Type: research

Ectopic gut colonization: a metagenomic study of the oral and gut microbiome in Crohn ’s disease
This study aims to characterize, the gut and oral microbiome in Asian subjects with Crohn ’s disease (CD) using whole genome shotgun sequencing, thereby allowing for strain-level comparison.MethodsA case –control study with age, sex and ethnicity matched healthy controls was conducted. CD subjects were limited to well-controlled patients without oral manifestations. Fecal and saliva samples were collected for characterization of gut and oral microbiome respectively. Microbial DNA were extracted, l ibraries prepared and sequenced reads profiled. Taxonomic diversity, taxonomic association, strain typing and micro...
Source: Gut Pathogens - February 25, 2021 Category: Microbiology Source Type: research

Are hospitalized patients with inflammatory bowel disease at increased risk of invasive bacterial infections? Results from POLIBD 3-year cohort study
AbstractThe aim of this study was to determine the dominant species of bacteria found in blood cultures collected from patients under treatment in the tertiary inflammatory bowel disease (IBD) center in Poland. The dominant pathogen isolated from blood in patients with IBD wasStaphylococcus epidermidis MRCNS (MRCNS —methicillin-resistant coagulase-negativeStaphylococcus), a strain resistant to all beta-lactam antibiotics (penicillins, penicillins with B-lactamase inhibitor, cephalosporins and carbapenems). The second most commonly isolated pathogen found in the blood samples wasEscherichia coli. Blood cultures were f...
Source: Gut Pathogens - February 22, 2021 Category: Microbiology Source Type: research

Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
ConclusionsOur analysis shows association of higherEnterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors. (Source: Gut Pathogens)
Source: Gut Pathogens - February 17, 2021 Category: Microbiology Source Type: research

Characteristics of patients infected with Clostridioides difficile at a Saudi Tertiary Academic Medical Center and assessment of antibiotic duration
ConclusionMost patients in this study had hospital-acquired non-severe CDI and were largely exposed to antibiotics and acid suppression therapy. Therefore, such therapies should be revised for necessity. (Source: Gut Pathogens)
Source: Gut Pathogens - February 17, 2021 Category: Microbiology Source Type: research

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj
ConclusionsThe whole genome sequences ofS. xiamenensis strain 111B and 111D, including comparative genomic analysis, highlight here the potential for virulence factors that might be related to the cause of diarrhea in humans and also indicate the possible acquisition of pathogenic bacteria, including antibiotic resistance genes or plasmids during the Hajj. (Source: Gut Pathogens)
Source: Gut Pathogens - February 9, 2021 Category: Microbiology Source Type: research

Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt
AbstractShiga toxin-producingEscherichia coli (STEC) is a pathotype ofE. coli that causes enteric and systemic diseases ranging from diarrhoea to severe hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The emergence of multidrug-resistant (MDR) STEC from cattle sources has increased public health risk and limited treatment options. The prevalence of STEC was investigated in 200 raw food samples (milk and beef samples) and 200 diarrheic samples (cattle and human samples) in a matched region. The presence ofstx genes (stx1 andstx2), carbapenemase-encoding genes (blaVIM,blaNDM-1, andblaIMP), and extended-spectrum...
Source: Gut Pathogens - February 5, 2021 Category: Microbiology Source Type: research

Detection of SARS-CoV-2 from patient fecal samples by whole genome sequencing
ConclusionThese results highlight the potential viability of SARS-CoV-2 in feces, its ongoing mutational accumulation, and its possible role in fecal –oral transmission. This study also elucidates the advantages of SARS-CoV-2 enrichment NGS, which may be a key methodology to document complete viral eradication.Trial registration ClinicalTrials.gov, NCT04359836, Registered 24 April 2020,https://clinicaltrials.gov/ct2/show/NCT04359836?term=NCT04359836&draw=2&rank=1). (Source: Gut Pathogens)
Source: Gut Pathogens - January 30, 2021 Category: Microbiology Source Type: research

The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air
ConclusionPhylogenetic analysis combined with detection of haemolytic (hblA, hblC, andhblD) and non-haemolytic (nheA, nheB, andnheC) enterotoxin genes in both air-isolated strains point to the diarrheic potential of the air isolates, though not emetic. Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air. (Source: Gut Pathogens)
Source: Gut Pathogens - January 30, 2021 Category: Microbiology Source Type: research

Adherent invasive Escherichia coli (AIEC) strain LF82, but not Candida albicans , plays a profibrogenic role in the intestine
ConclusionsThese results demonstrate that AIEC strain LF82, but notC. albicans, may play a major profibrogenic role in the gut. (Source: Gut Pathogens)
Source: Gut Pathogens - January 28, 2021 Category: Microbiology Source Type: research

Evaluation of CAMPYLOBACTER QUIK CHEK ™ rapid membrane enzyme immunoassay to detect Campylobacter spp. antigen in stool samples
AbstractCampylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method forCampylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK ™ (Abbott). The CRS used to c...
Source: Gut Pathogens - January 22, 2021 Category: Microbiology Source Type: research

Bacteroides , butyric acid and t10,c12-CLA changes in colorectal adenomatous polyp patients
ConclusionThe gut microbiota analysis results, assessed in faeces, showed thatBacteroides andCitrobacter were positively correlated with CAPs, which indicated that changes in specific genera might be detrimental to intestinal health. In addition,t10,c12-CLA played an important role in protecting the intestine. (Source: Gut Pathogens)
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research

High genetic diversity in Campylobacter concisus isolates from patients with microscopic colitis
AbstractThe emerging intestinal pathogenCampylobacter concisus has been associated with prolonged diarrhoea and classic inflammatory bowel diseases (IBD) and was recently also linked with microscopic colitis (MC). Previous reports have observed a high genetic diversity within isolates from diarrhoeic and IBD patients and from healthy controls (HC), and division of isolates into two major genomospecies (GS1 and GS2). The aim of this study was to describe genetic diversity in 80 recently cultivated MC biopsy and faecal isolates ofC. concisus by multi-locus sequence typing (MLST); and to compare the phylogenetic relatedness t...
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research

Eubacterium rectale contributes to colorectal cancer initiation via promoting colitis
ConclusionsOur findings reveal thatE. rectale functions as a ‘driver’ bacterium and contributes to cancer initiation via promoting inflammation. (Source: Gut Pathogens)
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research

Comparative genomics of Helicobacter pullorum from different countries
ConclusionIn this study, we obtained the genetic characteristics ofH. pullorum from different sources in the world. The comprehensive genetic characteristics ofH. pullorum were first described.H. pullorum showed highly genetic diversity and two sub-types of T6SSs were first identified inH. pullorum. 2013BJHL was found to be multidrug resistant as it was resistant to at least three different antibiotic classes. (Source: Gut Pathogens)
Source: Gut Pathogens - December 10, 2020 Category: Microbiology Source Type: research

Transcriptome changes and polymyxin resistance of acid-adapted Escherichia coli O157:H7 ATCC 43889
ConclusionThe transcriptomic and phenotypic changes analysed in this study during the adaptation ofE. coli to acid environments can provide useful information for developing intervention technologies and mitigating the risk associated with the emergence and spread of antimicrobial resistance. (Source: Gut Pathogens)
Source: Gut Pathogens - December 1, 2020 Category: Microbiology Source Type: research

Genomic patterns and characterizations of chromosomally-encoded mcr - 1 in Escherichia coli populations
In this study, we systematically investigated the genetic basis of chromosomally-encodedmcr-1 in prevalence and potential mechanisms of lineage, plasmid, insertion sequence, and phage. Our results demonstrated that the emergence of chromosomally-encodedmcr-1 could originate from multiple mechanisms, but mainly derived through the recombination of ISApl1/Tn6330. We reported a specific transmission mechanism, which is a phage-like region without lysogenic components, could associate with the emergence and stabilization of chromosomally-encodedmcr-1. These results highlighted the potential origin and risks of chromosomally-en...
Source: Gut Pathogens - November 28, 2020 Category: Microbiology Source Type: research

Emergence of plasmid-mediated mcr genes from Gram-negative bacteria at the human-animal interface
This study aimed to assess the emergence ofmcr gene-mediated Col-R in GNRs isolated from humans and animals in Pakistan.MethodsAnimal and clinical specimens collected from various sources were prospectively analysed using standard microbiological procedures. Pathogens were identified using the API 20E and API 20NE systems (bioMerieux). Minimum inhibitory concentration (MIC) against colistin was determined using the MIC detection methods, and multiplex polymerase chain reaction (PCR) was used to amplify themcr-1 tomcr-5 genes.Results We isolated 126 (88.1%) animal and 17 (11.9%) human Col-R phenotypes, among which there w...
Source: Gut Pathogens - November 20, 2020 Category: Microbiology Source Type: research

The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection
ConclusionThe study findings showed that the absence of mCRAMP modulated both host responses and the enteric microbiota enhancing local and systemic infection bySalmonella Typhimurium. (Source: Gut Pathogens)
Source: Gut Pathogens - November 13, 2020 Category: Microbiology Source Type: research

Stool and sputum microbiome during quinolone prophylaxis of spontaneous bacterial peritonitis: an exploratory study
ConclusionThe present exploratory study with small sample size showed that inter-individual differences in diversity of gut microbiota were high at baseline, yet quinolone prophylaxis had only a moderate impact. High relative abundances ofEnterobacteriaceae during follow-up might indicate failure of or non-adherence to quinolone prophylaxis. However, our results may not be clinically significant given the limitations of the study and therefore future studies are needed to further investigate this phenomenon. (Source: Gut Pathogens)
Source: Gut Pathogens - October 30, 2020 Category: Microbiology Source Type: research

Potential role of  intestinal microflora in disease progression among patients with different stages of Hepatitis B
ConclusionsFaecal microbiota transplantation of some potential beneficial bacteria can change with the occurrence of disease, and HBV carriers might be the most suitable donors. (Source: Gut Pathogens)
Source: Gut Pathogens - October 27, 2020 Category: Microbiology Source Type: research

A nineteen-year report of serotype and antimicrobial susceptibility of enteric non-typhoidal Salmonella from humans in Southern India: changing facades of taxonomy and resistance trend
AbstractBackgroundThe steady increase in the proportion of Non-typhoidalSalmonella (NTS) infections in humans represents a major health problem worldwide. The current study investigated the serovar distribution and antimicrobial susceptibility trends of NTS isolated from faecal samples during the period 2000 –2018.MethodsFaecal specimens of patients were cultured according to standard lab protocol. The isolates were serotyped and antimicrobial susceptibility testing (AST) were performed according to CLSI guidelines.ResultsA total of 1436 NTS isolates were obtained from faeces samples mostly comprising ofS. Typhimuriu...
Source: Gut Pathogens - October 23, 2020 Category: Microbiology Source Type: research

MiR-146a rs2910164 G   & gt;  C polymorphism modulates Notch-1/IL-6 signaling during infection: a possible risk factor for Crohn’s disease
This study was designed to investigate the impact of polymorphisms in miR146a on the immune response and infection in our MAP-CD model.MethodsWe determined the incidence of miR-146ars2910164 G  >  C in 42 blood samples from clinical CD patients and controls. We also measured the effect ofrs2910164 on expression of Notch-1 and IL-6, and plasma IL-6 protein levels in our study group. Finally, we analyzed the blood samples for MAP DNA and studied any correlation with miR-146a polymorphism. Samples were analyzed for statistical significance using unpaired tow-tailed t-test, unpaired two-tailed z-score and odds ratio. P  
Source: Gut Pathogens - October 15, 2020 Category: Microbiology Source Type: research

How to boost the immune defence prior to respiratory virus infections with the special focus on coronavirus infections
AbstractThe emergence of the novel coronavirus SARS-CoV-2, which causes severe respiratory tract infections in humans (COVID-19), has become a global health concern. One of the most worrying features of COVID-19 is a phenomenon known as the “cytokine storm”, which is a rapid overreaction of the immune system. Additionally, coagulation abnormalities, thrombocytopenia and digestive symptoms, including anorexia, vomiting, and diarrhea, are often observed in critically ill patients with COVID-19. Baker’s yeast β-glucan, a natural im munomodulatory component derived fromSaccharomyces cerevisiae, primes th...
Source: Gut Pathogens - October 12, 2020 Category: Microbiology Source Type: research

Fusobacterium nucleatum infection correlates with two types of microsatellite alterations in colorectal cancer and triggers DNA damage
AbstractFusobacterium nucleatum (Fn) is frequently found in colorectal cancers (CRCs). High loads ofFn DNA are detected in CRC tissues with microsatellite instability-high (MSI-H), or with theCpG island hypermethylation phenotype (CIMP).Fn infection is also associated with the inflammatory tumor microenvironment of CRC. A subtype of CRC exhibits inflammation-associated microsatellite alterations (IAMA), which are characterized by microsatellite instability-low (MSI-L) and/or an elevated level of microsatellite alterations at selected tetra-nucleotide repeats (EMAST). Here we describe two independent CRC cohorts in which he...
Source: Gut Pathogens - September 28, 2020 Category: Microbiology Source Type: research

In vivo animal models confirm an increased virulence potential and pathogenicity of the NAP1/RT027/ST01 genotype within the Clostridium difficile MLST Clade 2
ConclusionsDespite their close phylogenic relatedness, our results demonstrate differences in pathogenicity and virulence levels in Clade 2C. difficile strains, confirm the high severity of infections caused by the NAP1/RT027/ST01 strain, and highlight the importance ofC. difficile typing. (Source: Gut Pathogens)
Source: Gut Pathogens - September 21, 2020 Category: Microbiology Source Type: research

Plasmid mediated colistin resistant mcr -1 and co-existence of OXA -48 among Escherichia coli from clinical and poultry isolates: first report from Nepal
ConclusionsThe high prevalence of colistin and carbapenem resistant genes, and their co-existence in plasmid DNA ofE. coli isolates in this study suggests the possible spread to other animal, human and environmental pathogens. Molecular methods in addition to the conventional diagnostics in laboratories can help in early diagnosis, effective management and control of their potential transmission. (Source: Gut Pathogens)
Source: Gut Pathogens - September 16, 2020 Category: Microbiology Source Type: research

Mechanisms and microbial influences on CTLA-4 and PD-1-based immunotherapy in the treatment of cancer: a narrative review
ConclusionThe creation of oncomicrobiotics may allow many patients receiving anti-CTLA-4 and PD-1 immunotherapy to experience prolonged survival and a better quality of life. (Source: Gut Pathogens)
Source: Gut Pathogens - September 9, 2020 Category: Microbiology Source Type: research

Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection
ConclusionsSimilarity ofE. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenicE. faecalis strains. (Source: Gut Pathogens)
Source: Gut Pathogens - September 8, 2020 Category: Microbiology Source Type: research

The prophylactic effects of BIFICO on the antibiotic-induced gut dysbiosis and gut microbiota
AbstractBackgroundThe aim of this study is to evaluate the prophylactic effects of probiotic mixture BIFICO on antibiotic-induced gut dysbiosis (AIGD) and the influence on the change of the gut microbiota.MethodsWe conducted a prospective, randomized, controlled study and divided 196 patients who required intravenous beta-lactam antibiotics into three groups: a control group (no probiotics), a regular group (840  mg of BIFICO), and a double-dosage group (1680 mg of BIFICO). The symptoms of antibiotic-related diarrhea, bloating and abdominal pain and the incidence of AIGD were evaluated 7 days and 8–14&...
Source: Gut Pathogens - September 8, 2020 Category: Microbiology Source Type: research

Genome sequences of two clinical Escherichia coli isolates harboring the novel colistin-resistance gene variants mcr - 1.26 and mcr - 1.27
ConclusionsHere we present the draft sequences ofE. coli isolate 803-18 carrying the novel variantmcr-1.26 and isolate 844-14 carrying the novel variantmcr-1.27. The results highlight the increasing issue of transferable colistin resistance. (Source: Gut Pathogens)
Source: Gut Pathogens - September 2, 2020 Category: Microbiology Source Type: research

Efficacy of tailored second-line therapy of Helicobacter pylori eradication in patients with clarithromycin-based treatment failure: a multicenter prospective study
AbstractBackgroundAfter the failure of clarithromycin- and bismuth-based quadruple therapy (CBQT), levofloxacin- and bismuth-based quadruple therapy (LBQT) is recommended forHelicobacter pylori eradication. We compared the efficacies of second-line tailored bismuth-based quadruple therapy (TBQT) and empirical LBQT.MethodsPatients with CBQT failure were randomly assigned to receive TBQT or LBQT for 14  days. All patients underwent endoscopy for culture-based antibiotic susceptibility testing. Patients in the TBQT group exhibiting levofloxacin susceptibility were randomized to receive amoxicillin, levofloxacin, esomepra...
Source: Gut Pathogens - August 28, 2020 Category: Microbiology Source Type: research

Molecular characteristics of the VP1 region of enterovirus 71 strains in China
ConclusionThe C4 genotype of EV71 in China matches the vaccine and should effectively control EV71. However, the efficacy of the vaccine is partially affected by the continuous change in epidemic strains in Taiwan. These results suggest that the genetic characteristics of the EV71-VP1 region should be continuously monitored, which is critical for epidemic control and vaccine design to prevent EV71 infection in children. (Source: Gut Pathogens)
Source: Gut Pathogens - August 13, 2020 Category: Microbiology Source Type: research

Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease
ConclusionsCryptosporidium andCyclospora affected the bacterial structure at family and genus levels, decreasing the ratio between Firmicutes and Bacteroidetes in children with auto-antibodies for T1D or CD, which could increase the risk of illness onset. (Source: Gut Pathogens)
Source: Gut Pathogens - August 10, 2020 Category: Microbiology Source Type: research

Associations between polymorphisms in IL - 10 gene and the risk of viral hepatitis: a meta-analysis
ConclusionsThis meta-analysis demonstrates that rs1800871 ( − 819 C/T), rs1800872 (− 592 C/A) and rs1800896 (− 1082 G/A) polymorphisms may influence the risk of viral hepatitis in Asians, while only rs1800896 (− 1082 G/A) polymorphism may influence the risk of viral hepatitis in Caucasians. In further analyses by disease subtypes, we noticed that the three investigated polymorphisms may influence the risk of both HBV and HCV. (Source: Gut Pathogens)
Source: Gut Pathogens - July 27, 2020 Category: Microbiology Source Type: research

Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin
ConclusionsOur assay demonstrated high diagnostic sensitivity and specificity for detection of clarithromycin and levofloxacin resistantH. pylori. Based on proven accuracy, together with high efficiency, scalability and low cost, our assay has useful clinical utility for rapid diagnosis of clarithromycin and levofloxacin resistantH. pylori infections. Our assay results will provide patients with a clear diagnosis, enabling the treating clinician to administer the most effective antibiotic regimen to help the clear the infection. (Source: Gut Pathogens)
Source: Gut Pathogens - July 9, 2020 Category: Microbiology Source Type: research