Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan < em > Rosa roxburghii < /em > Tratt
CONCLUSIONS: Rosa roxburghii Tratt polysaccharides, consisting of D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, and L-fucose, possess the ability to hinder DU145 cell proliferation, migration, and invasion while inducing apoptosis through the modulation of relevant protein and gene expressions.PMID:38611854 | DOI:10.3390/molecules29071575 (Source: Cancer Control)
Source: Cancer Control - April 13, 2024 Category: Cancer & Oncology Authors: Ziyan Yang Guiyuan Chen Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: Molecular Medicine - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: Molecular Medicine - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: J Cell Mol Med - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: J Cell Mol Med - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
GSE256152 Transcriptomics and metabonomics study on the effect of combined exercise with curcumin supplementation on breast cancer in mice
This study constructed an anti breast tumor mouse model under the combined effects of curcumin treatment and swimming exercise. Transcriptomics and metabolomics techniques were used to screen differentially expressed genes and metabolites, evaluate the anti-cancer effect, and analyze the molecular regulatory mechanisms related to metabolism. Through the mouse phenotype observation, including the detection of tumor appearance, tumor imaging in vivo, and HE staining results of pathological sections, curcumin administration and exercise intervention combined treatment has more obvious inhibitory effect on breast cancer than a...
Source: GEO: Gene Expression Omnibus - April 10, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Evaluation of efficacy and safety of glucosamine sulfate, chondroitin sulfate, and their combination regimen in the management of knee osteoarthritis: a systematic review and meta-analysis
ConclusionThis meta-analysis revealed that the CS (with decreased pain intensity and improvement in the physical function), and GS (with significant reduction in the joint space narrowing) have significant therapeutic benefits. However, their combination did not significantly improve the symptoms or modify the disease. This may be due to the limited trials that are available on the combination of the sulfate forms of the intervention. Hence, there is a scope for conducting multicentric randomised controlled trials to evaluate and conclude the therapeutic role of CS and GS combination in the management of KOA. (Source: Inflammopharmacology)
Source: Inflammopharmacology - April 6, 2024 Category: Drugs & Pharmacology Source Type: research
< em > Isoptericola luteus < /em > sp. nov., a novel yellow bacterium isolated from soil
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006324.ABSTRACTAn aerobic, non-motile, Gram-stain-positive bacterium, designated strain NEAU-Y5T, was isolated from a soil sample collected from Northeast Agricultural University, Heilongjiang province. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Y5T belonged to the genus and showed high 16S rRNA sequence similarity to Isoptericola variabilis (98.9 %), Isoptericola nanjingensis (98.9 %), Isoptericola cucumis (98.5 %), Isoptericola hypogeus (98.5 %), Isoptericola dokdonensis (98.5 %), Isoptericola jiangsuensis (98.3 %), and...
Source: International Journal of Systematic and Evolutionary Microbiology - April 4, 2024 Category: Microbiology Authors: Hui Bing Ling Ling Jinzhao Gu Yang Cai Yance Zhang Lijie Cui Ailin Li Xinyu Kong Yanfang Luo Wensheng Xiang Junwei Zhao Source Type: research
< em > Isoptericola luteus < /em > sp. nov., a novel yellow bacterium isolated from soil
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006324.ABSTRACTAn aerobic, non-motile, Gram-stain-positive bacterium, designated strain NEAU-Y5T, was isolated from a soil sample collected from Northeast Agricultural University, Heilongjiang province. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Y5T belonged to the genus and showed high 16S rRNA sequence similarity to Isoptericola variabilis (98.9 %), Isoptericola nanjingensis (98.9 %), Isoptericola cucumis (98.5 %), Isoptericola hypogeus (98.5 %), Isoptericola dokdonensis (98.5 %), Isoptericola jiangsuensis (98.3 %), and...
Source: International Journal of Systematic and Evolutionary Microbiology - April 4, 2024 Category: Microbiology Authors: Hui Bing Ling Ling Jinzhao Gu Yang Cai Yance Zhang Lijie Cui Ailin Li Xinyu Kong Yanfang Luo Wensheng Xiang Junwei Zhao Source Type: research
Molecules, Vol. 29, Pages 1575: Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt
Conclusions: Rosa roxburghii Tratt polysaccharides, consisting of D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, and L-fucose, possess the ability to hinder DU145 cell proliferation, migration, and invasion while inducing apoptosis through the modulation of relevant protein and gene expressions. (Source: Molecules)
Source: Molecules - April 1, 2024 Category: Chemistry Authors: Ziyan Yang Guiyuan Chen Tags: Article Source Type: research
Analysis of molecular structure and topological properties of chitosan isolated from crab shell and mushroom
Int J Biol Macromol. 2024 Mar 21:131047. doi: 10.1016/j.ijbiomac.2024.131047. Online ahead of print.ABSTRACTThis investigation aimed to scrutinize the chemical and structural analogies between chitosan extracted from crab exoskeleton (High Molecular Weight Chitosan, HMWC) and chitosan obtained from mushrooms (Mushroom-derived Chitosan, MRC), and to assess their biological functionalities. The resulting hydrolysates from the hydrolysis of HMWC by chitosanase were categorized as chitosan oligosaccharides (csCOS), while those from MRC were denoted as mrCOS. The molecular weights (MW) of csCOS and mrCOS were determined using M...
Source: International Journal of Biological Macromolecules - March 23, 2024 Category: Biochemistry Authors: Eung Take Lee Jio Song Ji Hyun Lee Bon Guen Goo Jae Kweon Park Source Type: research
Analysis of molecular structure and topological properties of chitosan isolated from crab shell and mushroom
Int J Biol Macromol. 2024 Mar 21:131047. doi: 10.1016/j.ijbiomac.2024.131047. Online ahead of print.ABSTRACTThis investigation aimed to scrutinize the chemical and structural analogies between chitosan extracted from crab exoskeleton (High Molecular Weight Chitosan, HMWC) and chitosan obtained from mushrooms (Mushroom-derived Chitosan, MRC), and to assess their biological functionalities. The resulting hydrolysates from the hydrolysis of HMWC by chitosanase were categorized as chitosan oligosaccharides (csCOS), while those from MRC were denoted as mrCOS. The molecular weights (MW) of csCOS and mrCOS were determined using M...
Source: International Journal of Biological Macromolecules - March 23, 2024 Category: Biochemistry Authors: Eung Take Lee Jio Song Ji Hyun Lee Bon Guen Goo Jae Kweon Park Source Type: research
The Biodistribution and Utility of < sup > 99m < /sup > Tc-Ethylenedicysteine-Deoxyglucose ( < sup > 99m < /sup > Tc-Glucosamine) in the Identification of Active Disease in Patients with Rheumatoid Arthritis-a Single Center Prospective Study
CONCLUSION: 99mTc-ECDG can efficiently assess disease activity in the joints of patients with RA. It accumulates in sites of both clinical and subclinical disease and might be a very useful tool for the rheumatologist in the management of patients with RA.PMID:38510821 | PMC:PMC10948692 | DOI:10.1007/s13139-023-00823-4 (Source: Molecular Medicine)
Source: Molecular Medicine - March 21, 2024 Category: Molecular Biology Authors: Osayande Evbuomwan Barend Jansen Van Rensburg Gerrit Engelbrecht Cathryn H S Driver Mathys Labuschagne Joseph Sempa Je'nine Horn-Lodewyk Source Type: research
The Biodistribution and Utility of < sup > 99m < /sup > Tc-Ethylenedicysteine-Deoxyglucose ( < sup > 99m < /sup > Tc-Glucosamine) in the Identification of Active Disease in Patients with Rheumatoid Arthritis-a Single Center Prospective Study
CONCLUSION: 99mTc-ECDG can efficiently assess disease activity in the joints of patients with RA. It accumulates in sites of both clinical and subclinical disease and might be a very useful tool for the rheumatologist in the management of patients with RA.PMID:38510821 | PMC:PMC10948692 | DOI:10.1007/s13139-023-00823-4 (Source: Molecular Medicine)
Source: Molecular Medicine - March 21, 2024 Category: Molecular Biology Authors: Osayande Evbuomwan Barend Jansen Van Rensburg Gerrit Engelbrecht Cathryn H S Driver Mathys Labuschagne Joseph Sempa Je'nine Horn-Lodewyk Source Type: research
Use of Chitosan as a Precursor for Multiple Applications in Medicinal Chemistry: Recent Significant Contributions
This article reviews the main contributions published in the last ten years regarding the use and application of CS in medical chemistry. The applications exposed here involve regenerative medicine in the design of bioprocesses and tissue engineering, Pharmaceutical sciences to obtain biomaterials, polymers, biomedicine, and the use of nanomaterials and nanotechnology, toxicology, and Clinical Pharmaceuticals, emphasizing the perspectives and the direction that can take research in this area.PMID:38500287 | DOI:10.2174/0113895575275799240306105615 (Source: Mini Reviews in Medicinal Chemistry)
Source: Mini Reviews in Medicinal Chemistry - March 19, 2024 Category: Chemistry Authors: Diego Quiroga Carlos Coy-Barrera Source Type: research
