Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway
BMB Rep. 2023 Nov 14:6039. Online ahead of print.ABSTRACTElevation of blood glucose is associated with increased risk of atherosclerosis development. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although the rate of fatty acid uptake was not significantly changed. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, li...
Source: BMB Reports - November 15, 2023 Category: Biochemistry Authors: Sang-Min Kim Dong Yeol Kim Jiwon Park Young-Ah Moon Inn-Oc Han Source Type: research
GSE241313 Plasmodium falciparum MORC protein modulates gene expression through interaction with heterochromatin
Series Type : Expression profiling by high throughput sequencingOrganism : Plasmodium falciparumThe MORC (microrchidia) family of proteins is highly conserved in all eukaryotic cells and have been shown to play diverse roles by forming protein-protein interactions with immune-responsive proteins, SWI chromatin remodeling complexes, histone deacetylases, and histone tail modifications across metazoans. To dissect the functional roles of MORC in the human malaria parasite, Plasmodium falciparum, we developed a glmS based ribozyme knockdown system to induce PfMORC knockdown upon glucosamine (GlcN) induction. We further conduc...
Source: GEO: Gene Expression Omnibus - November 15, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Plasmodium falciparum Source Type: research
Conjugated linoleic acid and glucosamine supplements may prevent bone loss in aging by regulating the RANKL/RANK/OPG pathway
Mol Biol Rep. 2023 Nov 6. doi: 10.1007/s11033-023-08839-x. Online ahead of print.ABSTRACTThe skeleton is a living organ that undergoes constant changes, including bone formation and resorption. It is affected by various diseases, such as osteoporosis, osteopenia, and osteomalacia. Nowadays, several methods are applied to protect bone health, including the use of hormonal and non-hormonal medications and supplements. However, certain drugs like glucocorticoids, thiazolidinediones, heparin, anticonvulsants, chemotherapy, and proton pump inhibitors can endanger bone health and cause bone loss. New studies are exploring the us...
Source: Molecular Biology Reports - November 6, 2023 Category: Molecular Biology Authors: Shaymaa J Abdulrahman Mohanad Ali Abdulhadi Abduladheem Turki Jalil Dumooa Falah Muna S Merza Abbas F Almulla Ahmed Ali Ronak Taher Ali Source Type: research
A Review of Cartilage Defect Treatments Using Chitosan Hydrogels in Experimental Animal Models
CONCLUSION: In conclusion, CS hydrogels were able to stimulate tissue ingrowth and accelerate the process of cartilage repair in animal studies.PMID:37916637 | DOI:10.2174/0113892010245946230919062908 (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 2, 2023 Category: Biotechnology Authors: Lais Caroline Souza E Silva Cintia Cristina Santi Martignago Homero Garcia Motta Mirian Bonifacio Silva Ingrid Regina Avanzi L ívia Assis Daniel Araki Ribeiro Julia Risso Parisi Ana Claudia Renn ó Source Type: research
A Review of Cartilage Defect Treatments Using Chitosan Hydrogels in Experimental Animal Models
CONCLUSION: In conclusion, CS hydrogels were able to stimulate tissue ingrowth and accelerate the process of cartilage repair in animal studies.PMID:37916637 | DOI:10.2174/0113892010245946230919062908 (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 2, 2023 Category: Biotechnology Authors: Lais Caroline Souza E Silva Cintia Cristina Santi Martignago Homero Garcia Motta Mirian Bonifacio Silva Ingrid Regina Avanzi L ívia Assis Daniel Araki Ribeiro Julia Risso Parisi Ana Claudia Renn ó Source Type: research
Chemo-Enzymatic Synthesis of Isomeric I-branched Polylactosamines using Traceless Blocking Groups
Chemistry. 2023 Nov 1:e202302877. doi: 10.1002/chem.202302877. Online ahead of print.ABSTRACTPoly-N-acetyl lactosamines (polyLacNAc) are common structural motifs of N- and O-linked glycan, glycosphingolipids and human milk oligosaccharides. They can be branched by the addition of β1,6-linked N-acetyl-glucosamine (GlcNAc) moieties to internal galactoside (Gal) residues by the I-branching enzyme beta-1,6-N-acetylglucosaminyltransferase 2 (GCNT2). I-branching has been implicated in many biological processes and is also associated with various diseases such as cancer progression. Currently, there is a lack of methods that can...
Source: Chemistry - November 1, 2023 Category: Chemistry Authors: Ga ёl M Vos Yunfei Wu Roosmarijn van der Woude Robert P de Vries Geert-Jan Boons Source Type: research
Molecules, Vol. 28, Pages 7294: A New and Rapid HPLC Method to Determine the Degree of Deacetylation of Glutaraldehyde-Cross-Linked Chitosan
In this study, we prepare three calibration curves (with an R2 value over 0.92) for three series of reticulated polymers covering a large range of reticulation levels to demonstrate that a correlation can be established between the LR established via HPLC and the DDA obtained via titration. The polymers are derived from three different chitosan starting materials. These standard calibration curves are now used on a routine basis in our lab, and the HPLC method has allowed us to change our DDA analysis time from 20 h to 5 min. (Source: Molecules)
Source: Molecules - October 27, 2023 Category: Chemistry Authors: Ons Amamou Jean-Philippe Denis Élise Heinen Taoufik Boubaker S ébastien Cardinal Tags: Article Source Type: research
Acid hydrolysis conditions for quantification of meningococcal X polysaccharide in a pentavalent vaccine using HPAEC-PAD/ESI-MS
Anal Biochem. 2023 Oct 20:115363. doi: 10.1016/j.ab.2023.115363. Online ahead of print.ABSTRACTA selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from...
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Saurav Ghosh Rakesh Pawar Vivek Kangralkar Asha D Mallya Rajeev M Dhere Barbara Bolgiano Neil Ravenscroft Source Type: research
Acid hydrolysis conditions for quantification of meningococcal X polysaccharide in a pentavalent vaccine using HPAEC-PAD/ESI-MS
Anal Biochem. 2023 Oct 20:115363. doi: 10.1016/j.ab.2023.115363. Online ahead of print.ABSTRACTA selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from...
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Saurav Ghosh Rakesh Pawar Vivek Kangralkar Asha D Mallya Rajeev M Dhere Barbara Bolgiano Neil Ravenscroft Source Type: research
