Glycan-Reactive Innate-like B Cells and Developmental Checkpoints
J Immunol. 2024 Apr 22:ji2300587. doi: 10.4049/jimmunol.2300587. Online ahead of print.ABSTRACTUsing an Ig H chain conferring specificity for N-acetyl-d-glucosamine (GlcNAc), we developed transgenic (VHHGAC39 TG) mice to study the role of self-antigens in GlcNAc-reactive B-1 B cell development. In VHHGAC39 TG mice, GlcNAc-reactive B-1 B cell development during ontogeny and in adult bone marrow was normal. However, adult TG mice exhibited a block at transitional-2 immature B cell stages, resulting in impaired allelic exclusion and accumulation of a B cell subset coexpressing endogenous Ig gene rearrangements. Similarly, VHH...
Source: Journal of Immunology - April 22, 2024 Category: Allergy & Immunology Authors: J Stewart New Brian L P Dizon John F Kearney R Glenn King Source Type: research
Increasing outer membrane complexity: the case of the lipopolysaccharide lipid A from marine Cellulophaga pacifica
AbstractGram-negative bacteria living in marine waters have evolved peculiar adaptation strategies to deal with the numerous stress conditions that characterize aquatic environments. Among the multiple mechanisms for efficient adaptation, these bacteria typically exhibit chemical modifications in the structure of the lipopolysaccharide (LPS), which is a fundamental component of their outer membrane. In particular, the glycolipid anchor to the membrane of marine bacteria LPSs, i.e. the lipid A, frequently shows unusual chemical structures, which are reflected in equally singular immunological properties with potential appli...
Source: Glycoconjugate Journal - April 20, 2024 Category: Biochemistry Source Type: research
Functions and Metabolism of Amino Acids in Bones and Joints of Cats and Dogs
Adv Exp Med Biol. 2024;1446:155-175. doi: 10.1007/978-3-031-54192-6_7.ABSTRACTThe bone is a large and complex organ (12-15% of body weight) consisting of specialized connective tissues (bone matrix and bone marrow), whereas joints are composed of cartilage, tendons, ligaments, synovial joint capsules and membranes, and a synovial joint cavity filled with synovial fluid. Maintaining healthy bones and joints is a dynamic and complex process, as bone deposition (formation of new bone materials) and resorption (breakdown of the bone matrix to release calcium and phosphorus) are the continuous processes to determine bone balanc...
Source: Advances in Experimental Medicine and Biology - April 16, 2024 Category: Research Authors: Arianna N Lopez Fuller W Bazer Guoyao Wu Source Type: research
Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan < em > Rosa roxburghii < /em > Tratt
CONCLUSIONS: Rosa roxburghii Tratt polysaccharides, consisting of D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, and L-fucose, possess the ability to hinder DU145 cell proliferation, migration, and invasion while inducing apoptosis through the modulation of relevant protein and gene expressions.PMID:38611854 | DOI:10.3390/molecules29071575 (Source: Cancer Control)
Source: Cancer Control - April 13, 2024 Category: Cancer & Oncology Authors: Ziyan Yang Guiyuan Chen Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: Molecular Medicine - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: Molecular Medicine - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
Identification and characterization of novel genetic variants in the first Chinese family of mucopolysaccharidosis IIIC (Sanfilippo C syndrome)
In this study, we utilized whole-exome sequencing (WES) to identify novel compound heterozygous variants in the HGSNAT gene from a Chinese patient with typical MPS IIIC symptoms: c.743G>A; p.Gly248Glu and c.1030C>T; p.Arg344Cys. We performed in silico analysis and experimental validation, which confirmed the deleterious pathogenic nature of both variants, as evidenced by the loss of HGSNAT activity and failure of lysosomal localization. To the best of our knowledge, the MPS IIIC is first confirmed by clinical, biochemical and molecular genetic findings in China. Our study thus expands the spectrum of MPS IIIC pathoge...
Source: J Cell Mol Med - April 13, 2024 Category: Molecular Biology Authors: Hongjun Zhao Lijing Wang Mengfei Zhang Huakun Wang Sizhe Zhang Junjiao Wu Yu Tang Source Type: research
GSE256152 Transcriptomics and metabonomics study on the effect of combined exercise with curcumin supplementation on breast cancer in mice
This study constructed an anti breast tumor mouse model under the combined effects of curcumin treatment and swimming exercise. Transcriptomics and metabolomics techniques were used to screen differentially expressed genes and metabolites, evaluate the anti-cancer effect, and analyze the molecular regulatory mechanisms related to metabolism. Through the mouse phenotype observation, including the detection of tumor appearance, tumor imaging in vivo, and HE staining results of pathological sections, curcumin administration and exercise intervention combined treatment has more obvious inhibitory effect on breast cancer than a...
Source: GEO: Gene Expression Omnibus - April 10, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Evaluation of efficacy and safety of glucosamine sulfate, chondroitin sulfate, and their combination regimen in the management of knee osteoarthritis: a systematic review and meta-analysis
ConclusionThis meta-analysis revealed that the CS (with decreased pain intensity and improvement in the physical function), and GS (with significant reduction in the joint space narrowing) have significant therapeutic benefits. However, their combination did not significantly improve the symptoms or modify the disease. This may be due to the limited trials that are available on the combination of the sulfate forms of the intervention. Hence, there is a scope for conducting multicentric randomised controlled trials to evaluate and conclude the therapeutic role of CS and GS combination in the management of KOA. (Source: Inflammopharmacology)
Source: Inflammopharmacology - April 6, 2024 Category: Drugs & Pharmacology Source Type: research
< em > Isoptericola luteus < /em > sp. nov., a novel yellow bacterium isolated from soil
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006324.ABSTRACTAn aerobic, non-motile, Gram-stain-positive bacterium, designated strain NEAU-Y5T, was isolated from a soil sample collected from Northeast Agricultural University, Heilongjiang province. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Y5T belonged to the genus and showed high 16S rRNA sequence similarity to Isoptericola variabilis (98.9 %), Isoptericola nanjingensis (98.9 %), Isoptericola cucumis (98.5 %), Isoptericola hypogeus (98.5 %), Isoptericola dokdonensis (98.5 %), Isoptericola jiangsuensis (98.3 %), and...
Source: International Journal of Systematic and Evolutionary Microbiology - April 4, 2024 Category: Microbiology Authors: Hui Bing Ling Ling Jinzhao Gu Yang Cai Yance Zhang Lijie Cui Ailin Li Xinyu Kong Yanfang Luo Wensheng Xiang Junwei Zhao Source Type: research
< em > Isoptericola luteus < /em > sp. nov., a novel yellow bacterium isolated from soil
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006324.ABSTRACTAn aerobic, non-motile, Gram-stain-positive bacterium, designated strain NEAU-Y5T, was isolated from a soil sample collected from Northeast Agricultural University, Heilongjiang province. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Y5T belonged to the genus and showed high 16S rRNA sequence similarity to Isoptericola variabilis (98.9 %), Isoptericola nanjingensis (98.9 %), Isoptericola cucumis (98.5 %), Isoptericola hypogeus (98.5 %), Isoptericola dokdonensis (98.5 %), Isoptericola jiangsuensis (98.3 %), and...
Source: International Journal of Systematic and Evolutionary Microbiology - April 4, 2024 Category: Microbiology Authors: Hui Bing Ling Ling Jinzhao Gu Yang Cai Yance Zhang Lijie Cui Ailin Li Xinyu Kong Yanfang Luo Wensheng Xiang Junwei Zhao Source Type: research
Molecules, Vol. 29, Pages 1575: Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt
Conclusions: Rosa roxburghii Tratt polysaccharides, consisting of D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, and L-fucose, possess the ability to hinder DU145 cell proliferation, migration, and invasion while inducing apoptosis through the modulation of relevant protein and gene expressions. (Source: Molecules)
Source: Molecules - April 1, 2024 Category: Chemistry Authors: Ziyan Yang Guiyuan Chen Tags: Article Source Type: research
Analysis of molecular structure and topological properties of chitosan isolated from crab shell and mushroom
Int J Biol Macromol. 2024 Mar 21:131047. doi: 10.1016/j.ijbiomac.2024.131047. Online ahead of print.ABSTRACTThis investigation aimed to scrutinize the chemical and structural analogies between chitosan extracted from crab exoskeleton (High Molecular Weight Chitosan, HMWC) and chitosan obtained from mushrooms (Mushroom-derived Chitosan, MRC), and to assess their biological functionalities. The resulting hydrolysates from the hydrolysis of HMWC by chitosanase were categorized as chitosan oligosaccharides (csCOS), while those from MRC were denoted as mrCOS. The molecular weights (MW) of csCOS and mrCOS were determined using M...
Source: International Journal of Biological Macromolecules - March 23, 2024 Category: Biochemistry Authors: Eung Take Lee Jio Song Ji Hyun Lee Bon Guen Goo Jae Kweon Park Source Type: research
