Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05197-w. Online ahead of print.ABSTRACTHand, foot, and mouth disease (HFMD) caused by various enteroviruses is a major public health concern globally. Human enterovirus 71(EVA71), coxsackievirus A16 (CVA16), coxsackievirus A6 (CVA6), and coxsackievirus A10 (CVA10) are four major enteroviruses responsible for HFMD. Rapid, accurate, and specific point-of-care (POC) detection of the four enteroviruses is crucial for the prevention and control of HFMD. Here, we developed two multiplex high-fidelity DNA polymerase loop-mediated isothermal amplification (mHiFi-LAMP) assays ...
Source: Rural Remote Health - February 15, 2024 Category: Rural Health Authors: Xiaoling Zhang Yongjuan Zhao Xiaoyi Zhu Weimin Tian Chiyu Zhang Source Type: research
Porcine interferon- α linked to the porcine IgG-Fc induces prolonged and broad-spectrum antiviral effects against the foot-and-mouth disease virus
Antiviral Res. 2024 Feb 13:105836. doi: 10.1016/j.antiviral.2024.105836. Online ahead of print.ABSTRACTFoot-and-mouth disease (FMD) is an economically important disease, and the FMD virus (FMDV) can spread rapidly in susceptible animals. FMD is usually controlled through vaccination. However, commercial FMD vaccines are only effective 4-7 days after vaccination. Furthermore, FMDV comprises seven serotypes and various topotypes, and these aspects should be considered when selecting a vaccine. Antiviral agents could provide rapid and broad protection against FMDV. Therefore, this study aimed to develop a consensus porcine in...
Source: Antiviral Research - February 15, 2024 Category: Virology Authors: Gyeongmin Lee Aro Kim Hyo Rin Kang Ji-Hyeon Hwang Jong-Hyeon Park Min Ja Lee Byounghan Kim Su-Mi Kim Source Type: research
Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05197-w. Online ahead of print.ABSTRACTHand, foot, and mouth disease (HFMD) caused by various enteroviruses is a major public health concern globally. Human enterovirus 71(EVA71), coxsackievirus A16 (CVA16), coxsackievirus A6 (CVA6), and coxsackievirus A10 (CVA10) are four major enteroviruses responsible for HFMD. Rapid, accurate, and specific point-of-care (POC) detection of the four enteroviruses is crucial for the prevention and control of HFMD. Here, we developed two multiplex high-fidelity DNA polymerase loop-mediated isothermal amplification (mHiFi-LAMP) assays ...
Source: Analytical and Bioanalytical Chemistry - February 15, 2024 Category: Chemistry Authors: Xiaoling Zhang Yongjuan Zhao Xiaoyi Zhu Weimin Tian Chiyu Zhang Source Type: research
Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays
Anal Bioanal Chem. 2024 Feb 15. doi: 10.1007/s00216-024-05197-w. Online ahead of print.ABSTRACTHand, foot, and mouth disease (HFMD) caused by various enteroviruses is a major public health concern globally. Human enterovirus 71(EVA71), coxsackievirus A16 (CVA16), coxsackievirus A6 (CVA6), and coxsackievirus A10 (CVA10) are four major enteroviruses responsible for HFMD. Rapid, accurate, and specific point-of-care (POC) detection of the four enteroviruses is crucial for the prevention and control of HFMD. Here, we developed two multiplex high-fidelity DNA polymerase loop-mediated isothermal amplification (mHiFi-LAMP) assays ...
Source: Rural Remote Health - February 15, 2024 Category: Rural Health Authors: Xiaoling Zhang Yongjuan Zhao Xiaoyi Zhu Weimin Tian Chiyu Zhang Source Type: research
Porcine interferon- α linked to the porcine IgG-Fc induces prolonged and broad-spectrum antiviral effects against the foot-and-mouth disease virus
Antiviral Res. 2024 Feb 13:105836. doi: 10.1016/j.antiviral.2024.105836. Online ahead of print.ABSTRACTFoot-and-mouth disease (FMD) is an economically important disease, and the FMD virus (FMDV) can spread rapidly in susceptible animals. FMD is usually controlled through vaccination. However, commercial FMD vaccines are only effective 4-7 days after vaccination. Furthermore, FMDV comprises seven serotypes and various topotypes, and these aspects should be considered when selecting a vaccine. Antiviral agents could provide rapid and broad protection against FMDV. Therefore, this study aimed to develop a consensus porcine in...
Source: Antiviral Research - February 15, 2024 Category: Virology Authors: Gyeongmin Lee Aro Kim Hyo Rin Kang Ji-Hyeon Hwang Jong-Hyeon Park Min Ja Lee Byounghan Kim Su-Mi Kim Source Type: research
Porcine interferon- α linked to the porcine IgG-Fc induces prolonged and broad-spectrum antiviral effects against the foot-and-mouth disease virus
Antiviral Res. 2024 Feb 13:105836. doi: 10.1016/j.antiviral.2024.105836. Online ahead of print.ABSTRACTFoot-and-mouth disease (FMD) is an economically important disease, and the FMD virus (FMDV) can spread rapidly in susceptible animals. FMD is usually controlled through vaccination. However, commercial FMD vaccines are only effective 4-7 days after vaccination. Furthermore, FMDV comprises seven serotypes and various topotypes, and these aspects should be considered when selecting a vaccine. Antiviral agents could provide rapid and broad protection against FMDV. Therefore, this study aimed to develop a consensus porcine in...
Source: Antiviral Research - February 15, 2024 Category: Virology Authors: Gyeongmin Lee Aro Kim Hyo Rin Kang Ji-Hyeon Hwang Jong-Hyeon Park Min Ja Lee Byounghan Kim Su-Mi Kim Source Type: research
Feasibility of wearable monitors to detect heart rate variability in children with hand, foot and mouth disease
Hand foot and mouth disease (HFMD) is caused by a variety of enteroviruses, and occurs in large outbreaks in which a small proportion of children deteriorate rapidly with cardiopulmonary failure. Determining w... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - February 15, 2024 Category: Infectious Diseases Authors: Le Nguyen Thanh Nhan, Nguyen Thanh Hung, Truong Huu Khanh, Nguyen Thi Thu Hong, Nguyen Thi Han Ny, Le Nguyen Truc Nhu, Do Duong Kim Han, Tingting Zhu, Tran Tan Thanh, Girmaw Abebe Tadesse, David Clifton, H. Rogier Van Doorn, Le Van Tan and C. Louise Thwai Tags: Research Source Type: research
Incongruence between confirmed and suspected clinical cases of Japanese encephalitis virus infection
CONCLUSION: The results of this study indicate that there is a certain discrepancy between clinically diagnosed and laboratory-confirmed cases of JE. Each reported case should be based on laboratory detection results, which is of great importance in improving the accuracy of case diagnosis and reducing misreporting. Our results are not only important for addressing JE endemic to the Sichuan province, but also provide a valuable reference for the laboratory detection of various notifiable infectious diseases in China and other regions outside China.PMID:38343888 | PMC:PMC10853334 | DOI:10.3389/fcimb.2024.1302314 (Source: Herpes)
Source: Herpes - February 12, 2024 Category: Infectious Diseases Authors: Wei Li Yuliang Feng Hongrong Zhong Mingfeng Jiang Jiake Zhang Shihua Lin Na Chen Shusen He Kai Zhang Shihong Fu Huanyu Wang Guodong Liang Source Type: research
Effect of COVID-19 protective measures on the epidemiology characteristics of rotavirus, adenovirus, and coinfections among pediatric patients with acute gastroenteritis in Hangzhou, China
This study highlights the epidemiological changes of rotavirus (RV), adenovirus (AdV), and coinfections in children with acute gastroenteritis (AGE) before, during, and after coronavirus disease 2019 (COVID-19) periods. There was a highly statistically significant difference in the positive rates of RV-positive, AdV-positive, and coinfection (P < 0.001), indicating that RV remains the main pathogen causing AGE. It emphasizes the importance of continuous surveillance of RV and AdV at both local and global levels. Regular surveillance of prevalent rotavirus strains will facilitate the development of new inactivated rotavi...
Source: Adv Data - February 12, 2024 Category: Epidemiology Authors: Jianming Zhou Yanhong Sun Source Type: research
Inhibition of EV71 replication by an interferon-stimulated gene product L3HYPDH
Virus Res. 2024 Feb 13;342:199336. doi: 10.1016/j.virusres.2024.199336. Online ahead of print.ABSTRACTEnterovirus 71 (EV71) is the common causative agent of hand-foot-mouth disease (HFMD). Despite evidence in mice model suggested that the interferon (IFN) signaling pathways play a role in defending against this virus, knowledge on the IFN-mediated antiviral response is still limited. Here we identified an IFN-stimulated gene (ISG) called L3HYPDH, whose expression inhibits EV71 replication. Mapping assay indicated that amino acids 61-120 and 295-354 are critical for its optimal antiviral activity. Mechanismly, L3HYPDH speci...
Source: Virus Research - February 11, 2024 Category: Virology Authors: Jian Liu Logen Liu Shinuan Zeng Xiaobin Meng Nanfeng Lei Hai Yang Runcai Li Xin Mu Xuemin Guo Source Type: research
Diagnostic Accuracy of Cerebrospinal Fluid Multiplex Polymerase Chain Reaction Panel Testing in Patients With Suspected Central Nervous System Infections: A Multi-Center Study in the United Arab Emirates
Conclusion CSF mPCR panel is a highly sensitive and specific diagnostic tool for the diagnosis of CNS infections among all age groups in the UAE. Routine use of CSF mPCR panels can decrease healthcare costs by reducing the length of stay and can also aid antibiotic stewardship efforts by reducing antibiotic overuse in patients with viral CSF infections. CSF culture and mPCR complement each other by identifying CNS pathogens in patients with prior antibiotic exposure who would otherwise be missed if relying on CSF culture alone. However, concomitant CSF culture samples should be sent to avoid missing unusual CNS pathogens.P...
Source: Herpes - February 9, 2024 Category: Infectious Diseases Authors: Yousra Ghoweba Seyed Ali Safizadeh Shabestari Zainab A Malik Source Type: research
Simultaneous detection of dual microRNAs related to EV71 using ICP-MS based on metal nanoparticle labeling with hybridization chain reaction
We described a strategy for the simultaneous detection of miR296 and miR16 by ICP-MS based on metal nanoparticles (NPs) labeling with HCR. Briefly, single-stranded DNA (ssDNA) and magnetic beads (MBs), as well as NPs and signal probes for miRNA (Sp-miR) were firstly conjugated via the streptavidin-biotin recognition system, constituting ssDNA-MBs and NPs-Sp-miR complex, respectively. The latter complex then hybridized with the former through HCR, generating the nanosensors for targets. Then, the targets were added and hybridized with ssDNA, and the HCR complex with NPs was released into the solution. Finally, the correspon...
Source: Analytica Chimica Acta - February 9, 2024 Category: Chemistry Authors: Yuxin Wang Lijun Shao Zhigang Zhao Chao Huang Yanni Jiao Dapeng Sun Rui Liu Dafeng Jiang Xibao Gao Source Type: research
Multimodal Surveillance Model for Enterovirus D68 Respiratory Disease and Acute Flaccid Myelitis among Children in Colorado, USA, 2022
K. Messacar et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - February 5, 2024 Category: Infectious Diseases Source Type: research
Miltefosine reduces coxsackievirus B3 lethality of mice with enhanced STAT3 activation
This study was therefore designated to test the antiviral effects of miltefosine against CVB3 in vitro and especially, in mice, as few studies test miltefosine in vitro, but not in vivo. In vitro results showed that miltefosine inhibited viral replication with enhanced activation of the cellular transcription factor, STAT3, which is reported to reduce CVB3 both in vitro and in mice. Notably, STAT3 knockdown abolished the anti-CVB3 activity of miltefosine in vitro. Mouse studies demonstrated that miltefosine pretreatment reduced CVB3 lethality of mice with decreased virus loads, organ damage, and apoptosis, but enhanced STA...
Source: Antiviral Research - February 3, 2024 Category: Virology Authors: Chun Yu Zhang Cheng-Huei Hung Yi-Ling Hsiao Tung-Miao Chang Yu-Chieh Su Li-Chiu Wang Shih-Min Wang Shun-Hua Chen Source Type: research
Stability of enteroviruses on toys commonly found in kindergarten
Trop Biomed. 2023 Dec 1;40(4):478-485. doi: 10.47665/tb.40.4.014.ABSTRACTHand, foot, and mouth disease (HFMD) is a contagious childhood disease caused by enteroviruses including enterovirus A71 (EV-A71), coxsackievirus A6 (CV-A6) and CV-A16 transmitted via direct and indirect contact. Different types of toy surfaces can affect the stability of viruses. Understanding the stability of enteroviruses on toys provides insightful data for effective disinfection in kindergartens or homes. Porous (ethylene-vinyl acetate mat foam, paper, pinewood, polyester fabric, and squishy polyurethane foam) and non-porous (acrylonitrile butadi...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: S N A N Baharin S L Tan I C Sam Y F Chan Source Type: research
