Antibody response of sheep to simultaneous vaccination of foot-and-mouth disease, peste des petits ruminants, sheep pox and goat pox, and bluetongue vaccines
Trop Biomed. 2022 Mar 1;39(1):47-54. doi: 10.47665/tb.39.1.002.ABSTRACTThere are many infectious animal diseases in T urkey and generally, vaccination is the primarly control strategy to combat them. However, it is difficult to apply all vaccines in a definite period in the field due to limitations of the labor and finance. Rapid vaccination and effective use of labor can be possible with the help of simultaneous vaccine administrations. The study aims to show the effects of simultaneous foot-and-mouth disease (FMD), peste des petits ruminants (PPR), sheep pox and goat pox (SGP), and bluetongue (BT) vaccine administration ...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: B Sareyy üpoğlu G G ülyaz F Sara ç S Uzar Ö Kabaklı C Çokçalışkan C G ündüzalp E A Uzun O Camli Source Type: research

A new record of Rickettsia japonica in ticks infesting a Burmese ferret-badger in Thailand
In this study, we report the first case of Rickettsia japonica in Haemaphysalis hystricis tick collected from a roadkill Burmese ferret-badger ( Melogale personata ) in Loei province, northeastern Thailand. According to the DNA sequences and phylogenetic analyses of the outer membrane protein A and B genes ( ompA and ompB), the detected R. japonica was identical to those found in JSF patients in Korea, Japan, and China, and closely related to Rickettsia detected by ompA in a tick from Thailand. Further study on the prevalence of R. japonica and diversity of mammalian reservoir hosts will be useful to gain a better understa...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: S Hirunkanokpun A Ahantarig B Baimai P Pramual W Trinachartvanit Source Type: research

Description of the male of Simulium triglobus Takaoka & amp; Kuvangkadilok (Diptera: Simuliidae) from Thailand
In this study, the male of S. triglobus is described for the first time based on adult males reared from pupae collected from the type locality. The most distinctive characteristic of the male of S. triglobus is the shape of the ventral plate, which is hexagonal when viewed ventrally. No other members of S. multistriatum species group known thus far have such a unique ventral plate. In addition, the number of upper-eye (large) facets and color patterns of the legs can be used to differentiate this species in the male from other members of the S. multistriatum species-group. Mitochondrial cytochrome c oxidase I sequences en...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: P Pramual P Jomkumsing U Tangkawanit K Wongpakam N Bunchom H Takaoka Source Type: research

Genetic diversity of Duffy binding protein 2 region II ofPlasmodium cynomolgi from wild macaques in Peninsular Malaysia
This study investigates the genetic diversity, haplotypes, and natural selection of PcDBP2 region II from isolates collected from wild macaques in Peninsular Malaysia. Blood samples from 50 P. cynomolgi -infected wild macaques were used in the study. Genomic DNA extracted from the blood samples was used as template for PCR amplification of the PcDBP2 region II. The amplicons were cloned into a plasmid vector and sequenced. MEGA X and DnaSP ver.6.12.03 programmes were used to analyse the DNA sequences. A genealogical relationship of PcDBP2 region II were determined using haplotype network tree on NETWORK ver.10.2. Result sh...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: E N M Latif S Shahari A Amir F W Cheong Y L Lau M L Abdullah M Y Fong Source Type: research

Natural infection ofBlastocystis ST6 among commercial quails (Coturnix coturnix) in Penang, Malaysia
This study posits that the quail birds may serve as reservoirs of zoonotic subtypes of Blastocystis. More studies are required to understand the source of Blastocystis infection to poultry under intensive care and the role of poultry animals in the transmission of Blastocystis to humans.PMID:35507928 | DOI:10.47665/tb.39.1.012 (Source: Tropical Biomedicine)
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: A A Rauff-Adedotun S J Douglas A Rajamanikam Y Amira Suriaty M T Farah Haziqah Source Type: research

Descriptions of adults of Simulium (Gomphostilbia) sachini Takaoka & amp; Henry and S. ( G.) williei Takaoka & amp; Thapa in the S. (G.) gombakense species-group (Diptera: Simuliidae) from India
Trop Biomed. 2022 Mar 1;39(1):78-85. doi: 10.47665/tb.39.1.018.ABSTRACTThe Simulium gombakense species-group, one of the 15 species-groups of the subgenus Gomphostilbia Enderlein, is small, being represented by only 12 species, all of which are distributed in the Oriental Region. It is characterized by the pupal gill composed of an inflated structure and eight or ten slender filaments. Among the 12 species, S. sachini Takaoka & Henry, and S. williei Takaoka & Thapa were originally described from two pupae, and one pupa and two larvae, respectively, from Darjeeling, India. In the present paper, the adult females and...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: H Takaoka S Thapa S Chettri W Henry Source Type: research

Imported case ofLeishmania tropica cutaneous leishmaniasis in a 10-year-old child in Malaysia
Trop Biomed. 2022 Mar 1;39(1):86-88. doi: 10.47665/tb.39.1.017.ABSTRACTThe present paper reported a first imported case of cutaneous leishmaniasis in a 10-year- old child who returned from Saudi Arabia to Malaysia. Six weeks after his travel to Malaysia, two erythematous dermal nodules were developed over his right cheek and chin. Occurrence of intracellular amastigote of Leishmania was observed through examination of skin biopsy with hematoxylin and eosin stain. Furthermore, molecular analysis of ribosomal internal transcribed spacer 1 (ITS1) of Leishmania spp. confirmed the child was infected with Leishmania tropica. The...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: T K Tan N J Yap K F Leong C S Teh S T Tay Y A L Lim Source Type: research

Prevalence of Epstein-Barr virus infection in individuals with nasopharyngeal carcinoma in Malaysia: The first systematic review and meta-analysi
Trop Biomed. 2022 Mar 1;39(1):89-98. doi: 10.47665/tb.39.1.008.ABSTRACTEpstein-Barr virus (EBV) was the first herpesvirus associated to human malignancies. Despite the well-known association between EBV and malignancies, the prevalence of EBV infection in Malaysians with malignancies is unknown. Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) was used to conduct a systematic review and meta- analysis of published data in this study. Studies reporting the occurrence of EBV infection in Malaysian malignancy patients were searched in electronic databases like PubMed, Scopus, ScienceDirect, and Goog...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: E N S Engku Abd Rahman A A Irekeola R H Shueb R Mohamud N Mat Lazim B Abdullah Y Y Chan Source Type: research

Potential effect of methanol leaf extracts from three Podocarpus species on experimental cryptosporidiosis
This study aimed to examine the effect of methanol leaves extracts of the three Podocarpus species; P. macrophyllus (Thunb.), P. gracilior (Pilg.) and P. elongatus (Aiton) L' Hér. ex Pers and their combination on Cryptosporidium parvum (C. parvum) in experimentally infected mice in comparison with the commercially used drug, Nitazoxanide. As well as spectrophotometric estimation of the total phenolic and flavonoid content of these extracts was done. Results revealed that treatment with these three Podocarpus extracts and their combination showed a significant reduction of the number of C. parvum oocyst shed in the stool o...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: S S El-Hawary F N Kirollos K F Taha A A Dahab A A El-Mahis S H El-Sayed Source Type: research

Cryptosporidium species in HIV patients in Alexandria, Egypt: distribution and associated clinical findings
In conclusion, infection with Cryptosporidium sp. is common and frequently symptomatic in HIV patients in Egypt. The predominant species, C. hominis, C. parvum, and C. meleagridis show a distinct distribution in urban and rural residents.PMID:35507933 | DOI:10.47665/tb.39.1.013 (Source: Tropical Biomedicine)
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: M A Mohamed H M Hammam H A El-Taweel N F Abd El-Latif Source Type: research

Hematological and histopathological changes of rat's hearts experimentally infected with protoscoleces
This study was investigated the hematological and histopathological changes in the hearts of rats injected with protoscoleces. Rats were injected with protoscoleces collected from either liver of sheep, goats, and cows (from the abattoir of Al-Muthanna province, south of Iraq) or isolated from infected humans from Al-Hussein Teaching Hospital. Sheep protoscoleces showed a significant increase of lymphocytes that refer to the induction of a high response of the immune system in rats. The numbers of WBC, RBCs, and platelets were generally increased in rats injected with protoscoleces isolated from sheep and goats. These chan...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: M A Alsaadawi A H Al-Safar A M Al-Yasari H M Hussein A H Allawi N J Alsalih D A Kalef Source Type: research

The association of ABO and Rhesus blood type with the risks of developing SARS-CoV-2 infection: A meta-analysis
Trop Biomed. 2022 Mar 1;39(1):126-134. doi: 10.47665/tb.39.1.015.ABSTRACTCoronavirus Disease 2019 (COVID-19) has been spreading like a wildfire everywhere in the globe. It has been challenging the global health care system ever since the end of 2019, with its virulence and pathogenicity. Recent studies have shown the association between ABO blood group, Rhesus blood type and susceptibility to COVID-19 infection. Various studies and few meta-analyses have been done and some might be inconsistent; therefore, this meta-analysis was done to assess the relationship between different ABO and Rhesus blood types on the susceptibil...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: K-M Soo K M Chung M A A Mohd Azlan J Y Lam J W X Ren J J Arvind Y P Wong H Y Chee S Amin-Nordin Source Type: research

A conventional multiplex PCR for the detection of four common soil-transmitted nematodes in human feces: development and validation
Trop Biomed. 2022 Mar 1;39(1):135-143. doi: 10.47665/tb.39.1.016.ABSTRACTSoil-transmitted helminth (STH) infections, mainly caused by Ascaris lumbricoides, Trichuris trichiura, and hookworms, are among the most common intestinal parasites that infect humans. The infections are widely distributed throughout tropical and subtropical countries, including Malaysia, particularly in underprivileged communities. Microscopic and culture techniques have been used as a gold standard for diagnostic techniques. However, these methods yield low sensitivity and specificity, laborious and time-consuming. Therefore, simple, rapid, and acc...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: N A Hassan F A Noor Badi N Mohd-Shaharuddin W S Wan Yusoff Y A L Lim K H Chua S F N Sidi Omar L Y Chang H A Majid R Ngui Source Type: research

Efficacy of Plasmodium falciparum histidine-rich protein 2 ( Pfhrp 2) rapid diagnostic test (RDT) and microscopy in the detection of falciparum malaria among symptomatic patients in Akure, Nigeria
This study was designed to evaluate the efficacy of malaria histidine-rich protein 2-based rapid diagnostic test (RDT) and microscopy in the diagnosis of falciparum malaria in Nigeria. This was a cross-sectional and hospital-based study. The standard method of microscopy was used as the gold standard. Giemsa stained thick and thin smears were prepared to count and detect malaria parasite species. Also, a malaria histidine-rich protein 2-based RDT was used to detect malaria parasites and diagnostic efficacy were determined through the measure of sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive ...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: O B Awosolu Z S Yahaya M T Farah Haziqah Source Type: research

Emergence of resistance genes in fecal samples of antibiotic-treated Philippine broilers emphasizes the need to review local farming practices
This study surveyed antibiotic use in 12 commercial poultry farms in the Philippines. All farms reported the use of medically important antibiotics either for prophylaxis (91.67%), metaphylaxis (100%), and growth promotion (50%). Considering the widespread use of medically important antibiotics, we then investigated the potential contribution of local antibiotic treatment protocols to the emergence of antibiotic resistance genes in the fecal samples of broiler chickens under a controlled experimental setup. Significantly, we observed the emergence of antibiotic resistance genes ( ermB) in fecal samples of antibiotic-treate...
Source: Tropical Biomedicine - May 4, 2022 Category: Tropical Medicine Authors: I C Imperial P M Pabustan K A Valencia M A Nicdao J Ibana Source Type: research