Bovine anaplasmosis: A systematic review with meta-analysis on the global prevalence
This study aims to provide a comprehensive understanding of the global prevalence of bovine anaplasmosis and synthesized data from diverse geographic regions. A literature search was conducted to identify all relevant published articles reporting the prevalence of bovine anaplasmosis and a total of 164 studies were found eligible for final systematic review and meta-analysis. Meta-analysis was conducted using meta package of R software and summary estimates of the prevalence were calculated. Meta-analysis of 129,851 samples from 42 countries was conducted and the overall estimated prevalence of bovine anaplasmosis was foun...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Source Type: research
Molecular identification of Opisthorchis viverrini among the northeastern Cambodian population by internal transcribed spacer 2 based polymerase chain reaction
This study demonstrates that O. viverrini infection is distributed in rural areas located near freshwater reservoirs. Therefore, active surveillance, clinical examination of association with hepatobiliary, cholangiocarcinoma, and health education are needed.PMID:38308824 | DOI:10.47665/tb.40.4.002 (Source: Tropical Biomedicine)
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: N La M Leng P Arunsan P Pechdee A Boonsuya C Thanchonnang N K Rattanapitoon S K Rattanapitoon Source Type: research
Wild hosts and microscopic worlds: Investigating the morphology and surface ultrastructure of Blastocystis sp. in avian and non-human primate species
This study aimed to examine the morphology and the cell surface of Blastocystis in avian and non-human primates, including peafowl, pheasant, and lion-headed tamarin. By employing light microscopy and scanning electron microscopy (SEM), this study provides the first evidence of the cellular and surface features of Blastocystis in these animal species. Our findings revealed distinct variations in cell size, shape, and surface morphology among the different host species. Notably, the isolates from peafowl exhibited larger cell sizes compared to the isolates from the pheasant. However, interestingly, both animal species were ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: A Sanggari A O Attah Z S Yahaya M T Farah Haziqah Source Type: research
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge....
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Y Goa J G Du C Jirapattharasate E Galon S W Ji Z G Ran Y Q Xia Source Type: research
Cerebrospinal fluid inflammatory cytokine profiles of patients with neurotropic parasitic infections
In this study, cerebral spinal fluid (CSF) samples from suspected patients with parasitic infections were screened for pathogenic free-living amoebae by culture (n=2506) and PCR (n=275). Six proinflammatory cytokines in smear and culture-negative CSF samples from patients with GAE (n = 2), NCC (n = 7), and CT (n = 23) as well as control (n = 7) patients were measured using the Multiplex Suspension assay. None of the CSF samples tested was positive for neurotropic free-living amoebae by culture and only two samples showed Acanthamoeba 18S rRNA by PCR. Of the six cytokines measured, only IL-6 and IL-8 were significantly incr...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: D V John N Sreenivas H Deora M Purushottam M Debnath A Mahadevan S A Patil Source Type: research
