A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research
A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research
Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?
CONCLUSION: Interactions of EV-E with bovine immune cells may indicate potential evasion mechanisms of the virus. There is also a risk that an infection with this virus can predispose the organism to secondary infections, especially bacterial ones.PMID:38130447 | PMC:PMC10730555 | DOI:10.2478/jvetres-2023-0061 (Source: Cell Research)
Source: Cell Research - December 22, 2023 Category: Cytology Authors: Joanna Ma łaczewska Ma łgorzata Wróbel Edyta Kaczorek- Łukowska Wojciech R ękawek Source Type: research
Asymptomatic viruses detectable in saliva in the first year of life: a narrative review
Pediatr Res. 2023 Dec 22. doi: 10.1038/s41390-023-02952-0. Online ahead of print.ABSTRACTViral infections are common in children. Many can be asymptomatic or have delayed health consequences. In view of increasing availability of point-of-care viral detection technologies, with possible application in newborn screening, this review aimed to (1) identify potentially asymptomatic viruses detectable in infants under one year old, via saliva/nasopharyngeal swab, and (2) describe associations between viruses and long-term health conditions. We systematically searched Embase(Ovid), Medline(Ovid) and PubMed, then further searched...
Source: Pediatric Research - December 22, 2023 Category: Pediatrics Authors: Melody Goh Charissa Joy Alanna N Gillespie Qi Rui Soh Fan He Valerie Sung Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?
CONCLUSION: Interactions of EV-E with bovine immune cells may indicate potential evasion mechanisms of the virus. There is also a risk that an infection with this virus can predispose the organism to secondary infections, especially bacterial ones.PMID:38130447 | PMC:PMC10730555 | DOI:10.2478/jvetres-2023-0061 (Source: Cell Research)
Source: Cell Research - December 22, 2023 Category: Cytology Authors: Joanna Ma łaczewska Ma łgorzata Wróbel Edyta Kaczorek- Łukowska Wojciech R ękawek Source Type: research
Asymptomatic viruses detectable in saliva in the first year of life: a narrative review
Pediatr Res. 2023 Dec 22. doi: 10.1038/s41390-023-02952-0. Online ahead of print.ABSTRACTViral infections are common in children. Many can be asymptomatic or have delayed health consequences. In view of increasing availability of point-of-care viral detection technologies, with possible application in newborn screening, this review aimed to (1) identify potentially asymptomatic viruses detectable in infants under one year old, via saliva/nasopharyngeal swab, and (2) describe associations between viruses and long-term health conditions. We systematically searched Embase(Ovid), Medline(Ovid) and PubMed, then further searched...
Source: Pediatric Research - December 22, 2023 Category: Pediatrics Authors: Melody Goh Charissa Joy Alanna N Gillespie Qi Rui Soh Fan He Valerie Sung Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
Asymptomatic viruses detectable in saliva in the first year of life: a narrative review
Pediatr Res. 2023 Dec 22. doi: 10.1038/s41390-023-02952-0. Online ahead of print.ABSTRACTViral infections are common in children. Many can be asymptomatic or have delayed health consequences. In view of increasing availability of point-of-care viral detection technologies, with possible application in newborn screening, this review aimed to (1) identify potentially asymptomatic viruses detectable in infants under one year old, via saliva/nasopharyngeal swab, and (2) describe associations between viruses and long-term health conditions. We systematically searched Embase(Ovid), Medline(Ovid) and PubMed, then further searched...
Source: Pediatric Research - December 22, 2023 Category: Pediatrics Authors: Melody Goh Charissa Joy Alanna N Gillespie Qi Rui Soh Fan He Valerie Sung Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research
