Ehrlichiosis Presenting as Hemophagocytic Lymphohistiocytosis in an Immunocompetent Adult
Publication date: Available online 12 May 2020Source: IDCasesAuthor(s): Tarang Pankaj Patel, Phillip Beck, Dennis Chairman, Hariharan Regunath (Source: IDCases)
Source: IDCases - May 13, 2020 Category: Infectious Diseases Source Type: research
Tickborne Diseases: Diagnosis and Management.
Abstract
Tickborne diseases that affect patients in the United States include Lyme disease, Rocky Mountain spotted fever (RMSF), ehrlichiosis, anaplasmosis, babesiosis, tularemia, Colorado tick fever, and tickborne relapsing fever. Tickborne diseases are increasing in incidence and should be suspected in patients presenting with flulike symptoms during the spring and summer months. Prompt diagnosis and treatment can prevent complications and death. Location of exposure, identification of the specific tick vector, and evaluation of rash, if present, help identify the specific disease. Lyme disease presents ...
Source: American Family Physician - April 30, 2020 Category: Primary Care Authors: Pace EJ, O'Reilly M Tags: Am Fam Physician Source Type: research
Detection of Ehrlichia spp. and Anaplasma phagocytophilum in whole blood specimens using a duplex real-time PCR assay on the ARIES instrument
We report here the validation of a duplex real-time PCR assay using whole blood specimens on the Luminex ARIES® instrument, combining DNA extraction, amplification and detection into a one-step process. This method allows for rapid and sensitive detection of acute infections with Ehrlichia spp. and Anaplasma phagocytophilum using whole blood specimens. We included A. phagocytophilum to monitor emergence of this pathogen in Kentucky, since surrounding states have reported many more cases than Kentucky. (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - February 7, 2020 Category: Zoology Source Type: research
Discovery of in vivo Virulence Genes of Obligatory Intracellular Bacteria by Random Mutagenesis
Ehrlichia spp. are emerging tick-borne obligatory intracellular bacteria that cause febrile and sometimes fatal diseases with abnormal blood cell counts and signs of hepatitis. Ehrlichia HF strain provides an excellent mouse disease model of fatal human ehrlichiosis. We recently obtained and established stable culture of Ehrlichia HF strain in DH82 canine macrophage cell line, and obtained its whole genome sequence and annotation. To identify genes required for in vivo virulence of Ehrlichia, we constructed random insertional HF strain mutants by using Himar1 transposon-based mutagenesis procedure. Of total 158 insertional...
Source: Frontiers in cellular and infection microbiology - February 3, 2020 Category: Microbiology Source Type: research
“Detection of Ehrlichia spp. and Anaplasma phagocytophilum in whole blood specimens using a duplex real-time PCR assay on the ARIES instrument”
We report here the validation of a duplex real-time PCR assay using whole blood specimens on the Luminex ARIES® instrument, combining DNA extraction, amplification and detection into a one-step process. This method allows for rapid and sensitive detection of acute infections with Ehrlichia spp. and Anaplasma phagocytophilum using whole blood specimens. We included A. phagocytophilum to monitor emergence of this pathogen in Kentucky, since surrounding states have reported many more cases than Kentucky. (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - February 2, 2020 Category: Zoology Source Type: research
Ehrlichia canis TRP36 diversity in naturally infected-dogs from an urban area of Colombia
Publication date: Available online 7 January 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Esteban Arroyave, Juan D. Rodas-González, Xiaofeng Zhang, Marcelo B. Labruna, María S. González, Jorge A. Fernández-Silva, Jere W. McBrideAbstractEhrlichia canis is the etiologic agent of a highly prevalent tick-borne disease, canine monocytic ehrlichiosis (CME). Four defined E. canis genotypes based on the trp36 gene sequences have been reported, three of them identified in North or South America. The diversity of E. canis has been investigated using genetic and serologic approaches based on distinct 36 kDa tandem repeat...
Source: Ticks and Tick borne Diseases - January 25, 2020 Category: Zoology Source Type: research
Prevalence of Infection and Co-Infection and Presence of Rickettsial Endosymbionts in Ixodes Scapularis (Acari: Ixodidae) in Connecticut, USA.
Abstract
Ixodes scapularis is currently known to transmit 7 pathogens responsible for Lyme disease, anaplasmosis, babesiosis, tick-borne relapsing fever, ehrlichiosis, and Powassan encephalitis. Ixodes scapularis can also be colonized by endosymbiotic bacteria including those in the genus of Rickettsia. We screened 459 I. scapularis ticks submitted to the Connecticut Agricultural Experiment Station Tick Testing Laboratory with the objectives to (1) examine differences in infection prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, Babesia microti, and Borrelia miyamotoi, (2) evaluate whether pr...
Source: The Journal of Parasitology - January 25, 2020 Category: Parasitology Authors: Pokutnaya D, Molaei G, Weinberger DM, Vossbrinck CR, Diaz AJ Tags: J Parasitol Source Type: research
Ehrlichia canis TRP36 diversity in naturally infected-dogs from an urban area of Colombia
Publication date: Available online 7 January 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Esteban Arroyave, Juan D. Rodas-González, Xiaofeng Zhang, Marcelo B. Labruna, María S. González, Jorge A. Fernández-Silva, Jere W. McBrideAbstractEhrlichia canis is the etiologic agent of a highly prevalent tick-borne disease, canine monocytic ehrlichiosis (CME). Four defined E. canis genotypes based on the trp36 gene sequences have been reported, three of them identified in North or South America. The diversity of E. canis has been investigated using genetic and serologic approaches based on distinct 36 kDa tandem repeat...
Source: Ticks and Tick borne Diseases - January 8, 2020 Category: Zoology Source Type: research
My Mother's Story: Tick Borne Ehrlichiosis and a Life Well-Lived
Vector-Borne and Zoonotic Diseases, Ahead of Print. (Source: Vector-Borne and Zoonotic Diseases)
Source: Vector-Borne and Zoonotic Diseases - December 17, 2019 Category: Infectious Diseases Authors: Edward B. Breitschwerdt Ricardo G. Maggi Source Type: research
Genetic diversity of Anaplasma and Ehrlichia bacteria found in Dermacentor and Ixodes ticks in Mongolia
In this study, ticks were collected, identified, and pooled (n = 299) from three distinct environments across central Mongolia. Each pool was initially tested for Anaplasma/Ehrlichia using a 16S rRNA PCR assay that detects both genera, and specific PCR testing was done to identify those positive samples. Maximum likelihood estimation (MLE) of infection rates of ticks collected from the environment in Selenge aimag (province) found infection rates of Ixodes persulcatus ticks to be 2.0% (95% CI: 0.7, 4.3%) for A. phagocytophilum and 0.8% (95% CI: 0.1, 2.5%) for both nonspecific Ehrlichia and Anaplasma. Ehrlichia muris wa...
Source: Ticks and Tick borne Diseases - October 22, 2019 Category: Zoology Source Type: research
Chemiluminescent ELISA with multi-epitope proteins to improve the diagnosis of canine visceral leishmaniasis
In this study, we adapted a chemiluminescent enzyme-linked immunosorbent assay (CL ELISA), using two multi-epitope recombinant proteins (PQ10 and PQ20) and a crude Leishmania antigen produced using promastigotes of L. infantum, as antigens to detect CVL infection in animals from Belo Horizonte. To investigate cross-reactions, samples from dogs with other infections (babesiosis, ehrlichiosis and Trypanosoma cruzi) were tested. Assay performance validations were conducted to analyse parameters such as variability, reproducibility, and stability.CL ELISA sensitivity/specificity with PQ10 antigen was 93.1%/80.0%; with the PQ20...
Source: The Veterinary Journal - September 23, 2019 Category: Veterinary Research Source Type: research
Sequence Determinants Spanning −10 Motif and Spacer Region Implicated in Unique Ehrlichia chaffeensis Sigma 32-Dependent Promoter Activity of dnaK Gene
We reported that RNAP binding motifs of E. chaffeensis genes recognized by σ32 or σ70 share extensive homology and that transcription may be initiated by either one of the sigma factors, although transcriptional efficiencies differ. In the current study, we investigated mapping the E. chaffeensis dnaK gene promoter using the pathogen σ32 expressed in E. coli lacking its native σ32. The E. coli surrogate system and our previously described in vitro transcription system aided in defining the unique -10 motif and spacer sequence of the dnaK promoter. We also mapped σ32 amino acids/domains engaged in its promoter regulati...
Source: Frontiers in Microbiology - August 1, 2019 Category: Microbiology Source Type: research
Performance of recombinant chimeric proteins in the serological diagnosis of < i > Trypanosoma cruzi < /i > infection in dogs
Conclusions/SignificanceThe use of chimeric antigenic matrices in immunoassays for anti-T.cruzi IgG antibody detection in sera of infected dogs was shown to be a promising tool for veterinary diagnosis and epidemiological studies. The chimeric antigens used in this work allowed also to overcome the common hurdles related to serodiagnosis ofT.cruzi infection, especially regarding variation of efficiency parameters according to different strains and cross-reactivity with other infectious diseases. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - June 25, 2019 Category: Tropical Medicine Authors: Leonardo M. Leony Source Type: research
Tick-Borne Illness for Emergency Medicine Providers
AbstractPurpose of ReviewTicks are the most important vectors of human diseases after mosquitoes. Emergency physicians throughout the USA can be expected to encounter Lyme disease, Rocky Mountain spotted fever, ehrlichiosis, and other disorders based upon geographic location.Recent FindingsThe number of identifiable disorders transmitted by these arthropods has increased in recent years. Severe fever with thrombocytopenia syndrome has only been described within the past decade, and the new millennium has seen over 10 new species of rickettsial disease discovered.SummaryTicks transmit more pathogenic species than any other ...
Source: Current Emergency and Hospital Medicine Reports - June 12, 2019 Category: Emergency Medicine Source Type: research
Genetic diversity of Ehrlichia canis in dogs from Turkey inferred by TRP36 sequence analysis and phylogeny.
Abstract
Canine monocytic ehrlichiosis, an important tick-borne disease caused by Ehrlichia canis, is cosmopolitan but particularly prevalent in tropical and subtropical regions. In Turkey, the genetic diversity of E. canis remains undefined. The aim of this study was to characterize E. canis in naturally infected dogs from Turkey by sequencing and phylogenetic analysis of the Tandem Repeat Protein 36 (TRP36) encoded by the trp36 gene. A total of 167 archived blood samples randomly collected from municipal shelter dogs in three distinct geographic regions were analyzed for E. canis. Only ten samples (5.98%...
Source: Comparative immunology, microbiology and infectious diseases. - May 31, 2019 Category: Allergy & Immunology Authors: Aktas M, Özübek S Tags: Comp Immunol Microbiol Infect Dis Source Type: research
