QCM-based rapid detection of PCR amplification products of Ehrlichia canis.
Abstract
Ehrlichia canis is an intracellular parasitic bacterium and arthropod-borne pathogen that receives growing attention, because it leads to increasing morbidity and mortality in animals. It does so by causing canine monocytotropic ehrlichiosis (CME). Infected canines may lack obvious clinical signs and stay in chronic stage. Herein we report a rapid screening method based on PCR assay combined with quartz crystal microbalance (QCM) to design a DNA sensor for detecting E. canis in early stages of infection. The test relies on DNA amplification of target nucleotide sequences via PCR followed by detec...
Source: Analytica Chimica Acta - January 3, 2018 Category: Chemistry Authors: Bunroddith K, Viseshakul N, Chansiri K, Lieberzeit P Tags: Anal Chim Acta Source Type: research
Solution NMR structures of oxidized and reduced Ehrlichia chaffeensis thioredoxin: NMR-invisible structure owing to backbone dynamics
Thioredoxins are small ubiquitous proteins that participate in a diverse variety of redox reactions via the reversible oxidation of two cysteine thiol groups in a structurally conserved active site. Here, the NMR solution structures of a reduced and oxidized thioredoxin from Ehrlichia chaffeensis (Ec-Trx, ECH_0218), the etiological agent responsible for human monocytic ehrlichiosis, are described. The overall topology of the calculated structures is similar in both redox states and is similar to those of other thioredoxins: a five-stranded, mixed β -sheet ( β 1 – β 3 – β 2 – β 4 – β 5) surrounded by four α -...
Source: Acta Crystallographica Section F - December 23, 2017 Category: Biochemistry Authors: Buchko, G.W. Hewitt, S.N. Van Voorhis, W.C. Myler, P.J. Tags: human monocytic ehrlichiosis infectious diseases thioredoxin reactive oxygen species structural biology molecular dynamics Ehrlichia chaffeensis NMR research communications Source Type: research
Tick-borne infections.
Authors: Middleton DB
Abstract
Preview Is it because people are traveling more often or to more remote locations or because exposure to ticks is becoming more common? Whatever the reason, diseases spread by tick bites are being reported more often than in the past. Some of these, such as Rocky Mountain spotted fever and ehrlichiosis, can have very severe, even fatal, consequences. Dr Middleton describes findings that suggest tick-borne disease and summarizes effective treatment and prevention methods.
PMID: 29219644 [PubMed] (Source: Postgraduate Medicine)
Source: Postgraduate Medicine - December 10, 2017 Category: Internal Medicine Tags: Postgrad Med Source Type: research
Neospora caninum and Ehrlichia canis co ‐infection in a dog with meningoencephalitis
Abstract
An 8‐year‐old mixed‐breed dog was presented for acute, progressive weakness and ataxia, inappetence, and weight loss. The patient was mentally normal, but nonambulatory, with a right head tilt, right positional ventral strabismus, and slight head tremors. A neurologic lesion was localized to the cerebellum and right brainstem. Cerebrospinal fluid (CSF) analysis showed a markedly increased protein concentration and mixed pleocytosis, with eosinophil predominance (44%), intracytoplasmic inclusions within eosinophils, consistent with Ehrlichia canis (E canis) morulae, and Toxoplasma gondii (T gondii) or Neosp...
Source: Veterinary Clinical Pathology - December 1, 2017 Category: Veterinary Research Authors: Itamar Aroch, Gad Baneth, Harold Salant, Yaarit Nachum ‐Biala, Asaf Berkowitz, Merav Shamir, Orit Chai Tags: CASE REPORT Source Type: research
Changes in serum proteins in dogs with Ehrlichia canis infection
Publication date: December 2017 Source:Microbial Pathogenesis, Volume 113 Author(s): Damián Escribano, Hüseyin Cihan, Silvia Martínez-Subiela, Pinar Levent, Meric Kocaturk, Nilüfer Aytug, Jose Joaquin Cerón, Asta Tvarijonaviciute, Zeki Yilmaz The aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to...
Source: Microbial Pathogenesis - November 19, 2017 Category: Infectious Diseases Source Type: research
Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach
Publication date: 1 November 2017 Source:Preventive Veterinary Medicine, Volume 147 Author(s): Mitesh Mittal, Krishnendu Kundu, Soumendu Chakravarti, Jajati K. Mohapatra, Kapil Nehra, V.K. Sinha, B.S. Sanjeeth, C.P. Churamani, Anil Kumar Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking. ...
Source: Preventive Veterinary Medicine - October 28, 2017 Category: Veterinary Research Source Type: research
Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog
In this study, the 16S rRNA gene and the gp200 gene of E. canis were detected by polymerase chain reaction from an infected dog in the Philippines, and the deduced amino acid sequence of the gp200 gene was subjected to a phylogenetic analysis. The Philippine genotype formed a cluster with the Taiwan genotype, and was somewhat divergent from the USA and Brazil strains. This suggested that E. canis underwent evolution in East and Southeast Asia, confirming the utility of the gp200 gene for the assessment of genetic relationships among strains. (Source: Ticks and Tick borne Diseases)
Source: Ticks and Tick borne Diseases - September 21, 2017 Category: Zoology Source Type: research
Sudden sensorineural hearing loss in human monocytic ehrlichiosis.
We describe the onset of sudden sensorineural hearing loss in 2 patients with HME-a 31-year-old woman and an 82-year-old woman. The older patient experienced objective and subjective improvement in her hearing after treatment with an antibiotic and steroid taper; the younger patient was lost to audiologic follow-up. Additionally, we discuss the possible mechanisms of the hearing loss in these patients.
PMID: 28846788 [PubMed - in process] (Source: Ear, Nose and Throat Journal)
Source: Ear, Nose and Throat Journal - August 1, 2017 Category: ENT & OMF Authors: Bhalla V, Rodgers B, Lin J Tags: Ear Nose Throat J Source Type: research
First report of Ehrlichia infection in goats, China
Publication date: September 2017 Source:Microbial Pathogenesis, Volume 110 Author(s): Hui Zhang, Zhenyu Chang, Khalid Mehmood, Yajing Wang, Mujeeb Ur Rehman, Fazul Nabi, Ahamad Jawad Sabir, Xueting Liu, Xiaoxing Wu, Xinxin Tian, Donghai Zhou Ehrlichiosis is an emerging infectious disease of domestic animals which is transmitted by ticks. This disease has been reported earlier in most parts of China in dogs, cattle and humans, but there is no published data regarding this disease in goats. The present study provided the evidence of Ehrlichia infection in goats in Wuhan, China on the basis of clinical signs, gro...
Source: Microbial Pathogenesis - July 13, 2017 Category: Infectious Diseases Source Type: research
Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans.
Abstract
We have previously described a novel taxon of the genus Ehrlichia (type strain WisconsinT), closely related to Ehrlichia muris, that causes human ehrlichiosis among patients with exposures to ticks in the upper midwestern USA. DNA from this bacterium was also detected in Ixodes scapularis and Peromyscus leucopus collected in Minnesota and Wisconsin. To determine the relationship between the E. muris-like agent (EMLA) and other species of the genus Ehrlichia phenotypic, genotypic and epidemiologic comparisons were undertaken, including sequence analysis of eight gene loci (3906 nucleotides) for 39 ...
Source: International Journal of Systematic and Evolutionary Microbiology - July 12, 2017 Category: Microbiology Authors: Pritt BS, Allerdice MEJ, Sloan LM, Paddock CD, Munderloh UG, Rikihisa Y, Tajima T, Paskewitz SM, Neitzel DF, Hoang Johnson DK, Schiffman E, Davis JP, Goldsmith CS, Nelson CM, Karpathy SE Tags: Int J Syst Evol Microbiol Source Type: research
Serum antioxidant capacity and oxidative damage in clinical and subclinical canine ehrlichiosis
The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEACA), and the TEAC using the horseradish peroxidase (TEACH) were used. In addition, the serum thiol concentrations were analysed. Reactive oxygen species (ROS), thio...
Source: Research in Veterinary Science - June 17, 2017 Category: Veterinary Research Source Type: research
An ELISA immunoassay employing a conserved Leishmania hypothetical protein for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans.
Abstract
In the present study, a conserved Leishmania hypothetical protein, namely LiHypA, was evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans. This protein showed a high amino acid sequence homology between viscerotropic and cutaneotropic Leishmania species. An enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant antigen (rLiHypA), in addition to the A2 protein and two parasite antigenic preparations, which were used as controls. Regarding human diagnosis, results showed that rLiHypA was more sensitive and specific than ELISA-L. brazili...
Source: Cellular Immunology - June 8, 2017 Category: Allergy & Immunology Authors: Carvalho AMRS, Costa LE, Salles BCS, Santos TTO, Ramos FF, Lima MP, Chávez-Fumagalli MA, Silvestre BT, Portela ÁSB, Roatt BM, Silveira JAG, Gonçalves DU, Magalhães-Soares DF, Duarte MC, Menezes-Souza D, Coelho EAF Tags: Cell Immunol Source Type: research
High-dose filgrastim treatment of nonregenerative pancytopenia associated with chronic canine ehrlichiosis
To report the management and outcome of a dog with canine monocytic ehrlichiosis and nonregenerative pancytopenia with high doses of filgrastim. (Source: Topics in Companion Animal Medicine)
Source: Topics in Companion Animal Medicine - May 26, 2017 Category: Veterinary Research Authors: M. Palacios, R. Arteaga, G. Calvo Source Type: research
Relative Risk for Ehrlichiosis and Lyme Disease in an Area Where Vectors for Both Are Sympatric, New Jersey, USA
A. Egizi et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - May 11, 2017 Category: Infectious Diseases Source Type: research
Prevalence of Amblyomma gervaisi ticks on captive snakes in Tamil Nadu
AbstractTicks are the important ectoparasites that occur on snakes and transmit rickettsiosis, anaplasmosis and ehrlichiosis. A total of 62 snakes (Reticulated python, Indian Rock Python, Rat snakes and Spectacled cobra) were examined for tick infestation at Chennai Snake Park Trust (Guindy), Arignar Anna Zoological Park (Vandalur) and Rescue centre (Velachery) in Tamil Nadu from September, 2015 to June, 2016. Ticks from infested snakes were collected and were identified asAmblyomma gervaisi (previously known asAponomma gervaisi). Overall occurrence of tick infestation on snakes was 66.13%. Highest prevalence of tick infes...
Source: Journal of Parasitic Diseases - May 2, 2017 Category: Parasitology Source Type: research
