“Detection of Ehrlichia spp. and Anaplasma phagocytophilum in whole blood specimens using a duplex real-time PCR assay on the ARIES instrument”

We report here the validation of a duplex real-time PCR assay using whole blood specimens on the Luminex ARIES® instrument, combining DNA extraction, amplification and detection into a one-step process. This method allows for rapid and sensitive detection of acute infections with Ehrlichia spp. and Anaplasma phagocytophilum using whole blood specimens. We included A. phagocytophilum to monitor emergence of this pathogen in Kentucky, since surrounding states have reported many more cases than Kentucky.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research

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IJERPH, Vol. 17, Pages 5105: Spatio-Temporal Dynamics of Tick-Borne Diseases in North-Central Wisconsin from 2000–2016 International Journal of Environmental Research and Public Health doi: 10.3390/ijerph17145105 Authors: Austin Rau Claudia Munoz-Zanzi Anna M. Schotthoefer Jonathan D. Oliver Jesse D. Berman Lyme disease is a well-recognized public health problem in the USA, however, other tick-borne diseases also have major public health impacts. Yet, limited research has evaluated changes in the spatial and temporal patterns of non-Lyme tick-borne diseases within endemic regions. Using laboratory dat...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Abstract Ticks are the most important vectors of human pathogens, leading to increased public health burdens worldwide. Tick-borne pathogens include viruses (e.g. tick-borne encephalitis and Powassan); bacteria, such as the causative agents of Lyme disease, spotted fever rickettsiosis and human anaplasmosis; and malaria-like protozoan parasites causing babesiosis. Tick-borne diseases are emerging due to the geographical expansion of their tick vectors, especially in the northern hemisphere. Two examples of this phenomenon are Ixodes scapularis and Amblyomma americanum, which have expanded their ranges in the USA i...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En français : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse). J Obstet Gynaecol Can. 2020 May;42(5):644-653 Authors: Smith GN, Moore KM, Hatchette TF, Nicholson J, Bowie W, Langley JM Abstract OBJECTIVE: Lyme disease is an emerging infection in Canada caused by the bacterium belonging to the Borrelia burgdorferi sensu lato species complex, which is transmitted via the bite of an infected blacklegged tick. Populations of blacklegged ticks continue to expand and are now es...
Source: Journal of Obstetrics and Gynaecology Canada : JOGC - Category: OBGYN Tags: J Obstet Gynaecol Can Source Type: research
Abstract Tickborne diseases that affect patients in the United States include Lyme disease, Rocky Mountain spotted fever (RMSF), ehrlichiosis, anaplasmosis, babesiosis, tularemia, Colorado tick fever, and tickborne relapsing fever. Tickborne diseases are increasing in incidence and should be suspected in patients presenting with flulike symptoms during the spring and summer months. Prompt diagnosis and treatment can prevent complications and death. Location of exposure, identification of the specific tick vector, and evaluation of rash, if present, help identify the specific disease. Lyme disease presents with an ...
Source: American Family Physician - Category: Primary Care Authors: Tags: Am Fam Physician Source Type: research
We report here the validation of a duplex real-time PCR assay using whole blood specimens on the Luminex ARIES® instrument, combining DNA extraction, amplification and detection into a one-step process. This method allows for rapid and sensitive detection of acute infections with Ehrlichia spp. and Anaplasma phagocytophilum using whole blood specimens. We included A. phagocytophilum to monitor emergence of this pathogen in Kentucky, since surrounding states have reported many more cases than Kentucky.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Abstract Ixodes scapularis is currently known to transmit 7 pathogens responsible for Lyme disease, anaplasmosis, babesiosis, tick-borne relapsing fever, ehrlichiosis, and Powassan encephalitis. Ixodes scapularis can also be colonized by endosymbiotic bacteria including those in the genus of Rickettsia. We screened 459 I. scapularis ticks submitted to the Connecticut Agricultural Experiment Station Tick Testing Laboratory with the objectives to (1) examine differences in infection prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, Babesia microti, and Borrelia miyamotoi, (2) evaluate whether prevalence...
Source: The Journal of Parasitology - Category: Parasitology Authors: Tags: J Parasitol Source Type: research
(CBS News) – New government research shows that tickborne diseases like Lyme disease continue to rise. The report from the Centers for Disease Control and Prevention finds that in 2017, state and local health departments reported a record number of cases of illnesses spread by ticks. Cases of Lyme disease, Powassan virus; spotted fever rickettsiosis, including Rocky Mountain spotted fever, anaplasmosis, ehrlichiosis, babesiosis and tularemia all increased last year. The CDC said there were 59,349 confirmed cases of tickborne diseases in 2017, up from 48,610 in 2016. In past years, h...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Health News CBS News LYME DISEASE ticks Source Type: news
For most of us, springtime marks the return of life to a dreary landscape, bringing birdsong, trees in bud, and daffodils in bloom. But if you work for the Centers for Disease Control and Prevention (CDC), the coming of spring means the return of nasty diseases spread by ticks and mosquitoes. The killjoys at CDC celebrated the end of winter with a bummer of a paper showing that infections spread by ticks doubled in the United States from 2004 to 2016. (Tick populations have exploded in recent decades, perhaps due to climate change and loss of biodiversity.) Lyme disease The most common infection spread by ticks in the US i...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Infectious diseases Source Type: blogs
This article originally appeared on Health.com
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized healthytime onetime public health Source Type: news
The following data are abstracted from Gideon and the Gideon e-book series. [1]  Charts were created using an interactive tool driven by over 30,000 base graphs in the program. [2] Chart 1 contrasts trends for reported incidence of Lyme disease and Rocky Mountain spotted fever (RMSF).  Note that while rates of Lyme disease in 2016 are 15-fold those reported in 1987, those of RMSF increased by a factor of seven.  The number of fatal cases for both diseases have remained similar in recent years (i.e., the relative case-fatality rate of Lyme disease has decreased) Chart 2 summarizes incidence data for a variet...
Source: GIDEON blog - Category: Databases & Libraries Authors: Tags: Ebooks Epidemiology Graphs ProMED Source Type: blogs
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