Multi-Locus Sequence Typing of Ehrlichia chaffeensis Reveals Extensive Genotypic Variation across the United States
In this study, a molecular typing system was developed that allows for the genetic differentiation of E. chaffeensis isolates. This multi-locus typing system included sequencing and analyzing intergenic regions ECH0033-ECH0035 and ECH0217-ECH0218, plus, variable genes variable length PCR target, 28-kDa, 120-kDa, and hemE. We examined a total of 31 unique isolates from humans and white-tailed deer, and eight DNA samples extracted from infected A. americanum collected from multiple states. This is the largest evaluation of E. chaffeensis isolates and their genotypes. Our findings show that when sequences of all six loci were...
Source: The American Journal of Tropical Medicine and Hygiene - March 1, 2021 Category: Tropical Medicine Authors: Maria L Zambrano Christopher D Paddock Sandor E Karpathy Source Type: research
Multi-Locus Sequence Typing of Ehrlichia chaffeensis Reveals Extensive Genotypic Variation across the United States
In this study, a molecular typing system was developed that allows for the genetic differentiation of E. chaffeensis isolates. This multi-locus typing system included sequencing and analyzing intergenic regions ECH0033-ECH0035 and ECH0217-ECH0218, plus, variable genes variable length PCR target, 28-kDa, 120-kDa, and hemE. We examined a total of 31 unique isolates from humans and white-tailed deer, and eight DNA samples extracted from infected A. americanum collected from multiple states. This is the largest evaluation of E. chaffeensis isolates and their genotypes. Our findings show that when sequences of all six loci were...
Source: Am J Trop Med Hyg - March 1, 2021 Category: Infectious Diseases Authors: Maria L Zambrano Christopher D Paddock Sandor E Karpathy Source Type: research
Multi-Locus Sequence Typing of Ehrlichia chaffeensis Reveals Extensive Genotypic Variation across the United States
In this study, a molecular typing system was developed that allows for the genetic differentiation of E. chaffeensis isolates. This multi-locus typing system included sequencing and analyzing intergenic regions ECH0033-ECH0035 and ECH0217-ECH0218, plus, variable genes variable length PCR target, 28-kDa, 120-kDa, and hemE. We examined a total of 31 unique isolates from humans and white-tailed deer, and eight DNA samples extracted from infected A. americanum collected from multiple states. This is the largest evaluation of E. chaffeensis isolates and their genotypes. Our findings show that when sequences of all six loci were...
Source: The American Journal of Tropical Medicine and Hygiene - March 1, 2021 Category: Tropical Medicine Authors: Maria L Zambrano Christopher D Paddock Sandor E Karpathy Source Type: research
Anaplasma Infection in Ticks in Southeastern Region of Iran.
Conclusion: This study shows high infection rates to Anaplasma in hard ticks. It is essential for health and veterinary authorities and farmers to use appropriate strategies to control ticks to reduce the infestation.
PMID: 33365340 [PubMed] (Source: Iranian Journal of Arthropod-Borne Diseases)
Source: Iranian Journal of Arthropod-Borne Diseases - December 30, 2020 Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Comparison of Anaplasma and Ehrlichia species-specific peptide ELISAs with whole organism-based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs.
CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that peptide-based ELISAs had enhanced specificity relative to whole organism-based IFAs for detection of antibodies against Anaplasma and Ehrlichia spp, which should facilitate accurate diagnosis and may help detect dogs coinfected with multiple TBPs.
PMID: 33369490 [PubMed - as supplied by publisher] (Source: American Journal of Veterinary Research)
Source: American Journal of Veterinary Research - December 29, 2020 Category: Veterinary Research Authors: Qurollo BA, Stillman BA, Beall MJ, Foster P, Hegarty BC, Breitschwerdt EB, Chandrashekar R Tags: Am J Vet Res Source Type: research
Human Monocytic Ehrlichiosis, Mexico City, Mexico.
We report a fatal case of human monocytic ehrlichiosis in Mexico City in a man who was homeless.
PMID: 33219798 [PubMed - as supplied by publisher] (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - November 23, 2020 Category: Infectious Diseases Authors: Alcántara-Rodríguez VE, Sánchez-Montes S, Contreras H, Colunga-Salas P, Fierro-Flores L, Avalos S, Rodríguez-Rangel F, Becker I, Walker DH Tags: Emerg Infect Dis Source Type: research
Human Monocytic Ehrlichiosis, Mexico City, Mexico
V. E. Alc ántara-Rodríguez et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - November 8, 2020 Category: Infectious Diseases Source Type: research
Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis
Nowadays, Ehrlichia canis receives increasing attention because of its great morbidity and mortality in animals. Dogs in the subclinical and chronic phases can be asymptomatic, and serological tests show cross-re... (Source: Parasites and Vectors)
Source: Parasites and Vectors - October 15, 2020 Category: Microbiology Authors: Carlos A. Rodr íguez-Alarcón, Diana M. Beristain-Ruiz, Angélica Olivares-Muñoz, Andrés Quezada-Casasola, Federico Pérez-Casio, Jesús A. Álvarez-Martínez, Jane Tapia-Alanís, José J. Lira-Amaya, Ramón Rivera-Barreno, Orlando S. Cera-Hurtado, Jos Tags: Research Source Type: research
Ehrlichia chaffeensis EplA Interaction With Host Cell Protein Disulfide Isomerase Promotes Infection
In this study, we identified ECH_0377, henceforth designated as EplA (E. chaffeensis PDI ligand A) as an E. chaffeensis adhesin that interacts with host cell protein disulfide isomerase (PDI) to mediate bacterial entry into host cells. EplA is an outer membrane protein that E. chaffeensis expresses during growth in THP-1 monocytic cells. Canine sera confirmed to be positive for exposure to Ehrlichia spp. recognized recombinant EplA, indicating that it is expressed during infection in vivo. EplA antiserum inhibited the bacterium's ability to infect monocytic cells. The EplA-PDI interaction was confirmed via co-immunoprecipi...
Source: Frontiers in cellular and infection microbiology - September 22, 2020 Category: Microbiology Source Type: research
Multiple Ehrlichia chaffeensis Genes Critical for Its Persistent Infection in a Vertebrate Host Are Identified by Random Mutagenesis Coupled with In Vivo Infection Assessment [Cellular Microbiology: Pathogen-Host Cell Molecular Interactions]
We reported earlier that the Himar1 transposon-based random mutagenesis is a valuable tool in defining E. chaffeensis genes critical for its persistent growth in vivo in reservoir and incidental hosts. The method also aided in extending studies focused on vaccine development and immunity. Here, we describe the generation and mapping of 55 new mutations. To define the critical nature of the bacterial genes, infection experiments were carried out in the canine host with pools of mutant organisms. Infection evaluation in the physiologically relevant host by molecular assays and by xenodiagnoses allowed the identification of m...
Source: Infection and Immunity - September 17, 2020 Category: Infectious Diseases Authors: Wang, Y., Nair, A. D. S., Alhassan, A., Jaworski, D. C., Liu, H., Trinkl, K., Hove, P., Ganta, C. K., Burkhardt, N., Munderloh, U. G., Ganta, R. R. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
Optimization and Evaluation of a Multiplex Quantitative PCR Assay for Detection of Nucleic Acids in Human Blood Samples from Patients with Spotted Fever Rickettsiosis, Typhus Rickettsiosis, Scrub Typhus, Monocytic Ehrlichiosis, and Granulocytic Anaplasmosis [Chlamydiology and Rickettsiology]
Spotted fever group rickettsioses (SFGR), typhus group rickettsioses (TGR), scrub typhus (caused by Orientia tsutsugamushi), ehrlichiosis, and anaplasmosis often present as undifferentiated fever but are not treated by agents (penicillins and cephalosporins) typically used for acute febrile illness. Inability to diagnose these infections when the patient is acutely ill leads to excess morbidity and mortality. Failure to confirm these infections retrospectively if a convalescent blood sample is not obtained also impairs epidemiologic and clinical research. We designed a multiplex real-time quantitative PCR (qPCR) assay to d...
Source: Journal of Clinical Microbiology - August 23, 2020 Category: Microbiology Authors: Reller, M. E., Dumler, J. S. Tags: Chlamydiology and Rickettsiology Source Type: research
IJERPH, Vol. 17, Pages 5105: Spatio-Temporal Dynamics of Tick-Borne Diseases in North-Central Wisconsin from 2000 –2016
IJERPH, Vol. 17, Pages 5105: Spatio-Temporal Dynamics of Tick-Borne Diseases in North-Central Wisconsin from 2000–2016
International Journal of Environmental Research and Public Health doi: 10.3390/ijerph17145105
Authors:
Austin Rau
Claudia Munoz-Zanzi
Anna M. Schotthoefer
Jonathan D. Oliver
Jesse D. Berman
Lyme disease is a well-recognized public health problem in the USA, however, other tick-borne diseases also have major public health impacts. Yet, limited research has evaluated changes in the spatial and temporal patterns of non-Lyme tick-borne diseases within endemic regions. Using laboratory data fr...
Source: International Journal of Environmental Research and Public Health - July 14, 2020 Category: Environmental Health Authors: Austin Rau Claudia Munoz-Zanzi Anna M. Schotthoefer Jonathan D. Oliver Jesse D. Berman Tags: Article Source Type: research
Emerging tick-borne pathogens of public health importance: a mini-review.
Abstract
Ticks are the most important vectors of human pathogens, leading to increased public health burdens worldwide. Tick-borne pathogens include viruses (e.g. tick-borne encephalitis and Powassan); bacteria, such as the causative agents of Lyme disease, spotted fever rickettsiosis and human anaplasmosis; and malaria-like protozoan parasites causing babesiosis. Tick-borne diseases are emerging due to the geographical expansion of their tick vectors, especially in the northern hemisphere. Two examples of this phenomenon are Ixodes scapularis and Amblyomma americanum, which have expanded their ranges in t...
Source: Journal of Medical Microbiology - May 31, 2020 Category: Microbiology Authors: Rochlin I, Toledo A Tags: J Med Microbiol Source Type: research
High Performance of ELISA test using recombinant rLiNTPDase2 from Leishmania infantum: a Phase II diagnosis of Canine Visceral Leishmaniasis.
In this study, we aimed to evaluate rLiNTPDase2-ELISA in a Phase II study with 651 dog sera samples, also comparing it with methodologies previously established and used in epidemiology surveillance in Brazil, an endemic country of CVL and VL. The rLiNTPDase2-ELISA using standard control sera showed high capability to distinguish between positive and negative sera, sensitivity of 92.6% and specificity of 88.5%. The test was reproductive and the kappa statistics judgement "substantial agreement". rLiNTPDase2-ELISA does not show cross-reactivity with ehrlichiosis-reagent sera. However, we verified 15.3% of cross-reactivity w...
Source: Acta Tropica - May 21, 2020 Category: Infectious Diseases Authors: de Souza ACA, de Castro RB, Luciolo Dos Santos Y, Pavione NDRT, Agripino JM, Bahia MT, Machado-Coelho GLL, de Souza RF, de Oliveira LL, de Souza CDSF, Bressan GC, Vasconcellos RS, de Almeida MR, Fietto JLR Tags: Acta Trop Source Type: research
Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En fran çais : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse).
Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En français : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse).
J Obstet Gynaecol Can. 2020 May;42(5):644-653
Authors: Smith GN, Moore KM, Hatchette TF, Nicholson J, Bowie W, Langley JM
Abstract
OBJECTIVE: Lyme disease is an emerging infection in Canada caused by the bacterium belonging to the Borrelia burgdorferi sensu lato species complex, which is transmitted via the bite of an infected blacklegged tick. Populations of blacklegged ticks continue to expan...
Source: Journal of Obstetrics and Gynaecology Canada : JOGC - May 18, 2020 Category: OBGYN Tags: J Obstet Gynaecol Can Source Type: research
