Structural homology of mite profilins to plant profilins is not indicative of allergic cross-reactivity
Biol Chem. 2024 Apr 26. doi: 10.1515/hsz-2023-0366. Online ahead of print.ABSTRACTStructural and allergenic characterization of mite profilins has not been previously pursued to a similar extent as plant profilins. Here, we describe structures of profilins originating from Tyrophagus putrescentiae (registered allergen Tyr p 36.0101) and Dermatophagoides pteronyssinus (here termed Der p profilin), which are the first structures of profilins from Arachnida. Additionally, the thermal stabilities of mite and plant profilins are compared, suggesting that the high number of cysteine residues in mite profilins may play a role in ...
Source: Biological Chemistry - April 25, 2024 Category: Chemistry Authors: Andrea O'Malley Sahana Sankaran Avery Carriuolo Kriti Khatri Krzysztof Kowal Maksymilian Chruszcz Source Type: research
Exploring the role of coagulation-related genes in renal cell carcinoma: Implications for tumor microenvironment and prognostic biomarkers
CONCLUSION: There is an obvious correlation between the coagulation and the tumor microenvironment in ccRCC. As a key coagulation-related gene, MMP9 may promote the progression of renal cell carcinoma by influencing immune infiltration of CD8+T cells and Treg cells. Additionally, the risk score could be used as a durable prognostic biomarker, which could assist in clinical decision making for ccRCC patients.PMID:38663187 | DOI:10.1016/j.compbiolchem.2024.108082 (Source: Computational Biology and Chemistry)
Source: Computational Biology and Chemistry - April 25, 2024 Category: Bioinformatics Authors: Yang Qiu Yuanpeng Liao Jianqiang Zhang Yuedian Ye Zhongshu Zhang Zheng Jiang Jiaai Zhang Jinghan Xin Shidong Lv Hongmei Peng Source Type: research
Synthesis and evaluation of pentacyclic triterpenoids conjugates as novel HBV entry inhibitors targeting NTCP receptor
In this study, pentacyclic triterpenoids (PTs) including glycyrrhetinic acid (GA), oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA) were modified via molecular hybridization with podophyllotoxin respectively, and resulted in thirty-two novel conjugates. The anti-HBV activities of conjugates were evaluated in HepG2.2.15 cells. The results showed that 66% of the conjugates exhibited lower toxicity to the host cells and had significant inhibitory effects on the two HBV antigens, especially HBsAg. Notably, the compounds BA-PPT1, BA-PPT3, BA-PPT4, and UA-PPT3 not only inhibited the secretion of HBsAg but also supp...
Source: Bioorganic Chemistry - April 25, 2024 Category: Chemistry Authors: Yixin Chen Meitao Duan Xiangwan Wang Jianling Xu Shuo Tian Xiaotian Xu Ao Duan Ahmed Mahal Yongyan Zhu Quanhong Zhu Source Type: research
Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research
Protein engineering for the synthetic cell factory: Recent advance and perspective
Biotechnol Adv. 2024 Apr 23:108366. doi: 10.1016/j.biotechadv.2024.108366. Online ahead of print.ABSTRACTSynthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles. Protein engineering is a technology for creating novel protein variants with desired properties and functions by modify...
Source: Biotechnology Advances - April 25, 2024 Category: Biotechnology Authors: Ailin Guan Zixi He Xin Wang Zhi-Jun Jia Jiufu Qin Source Type: research
Proline Peptide Bond Isomerization in Ubiquitin under Folding and Denaturing Conditions by Pressure-Jump NMR
J Mol Biol. 2024 Apr 23:168587. doi: 10.1016/j.jmb.2024.168587. Online ahead of print.ABSTRACTProline isomerization is widely recognized as a kinetic bottleneck in protein folding, amplified for proteins rich in Pro residues. We introduced repeated hydrostatic pressure jumps between native and pressure-denaturing conditions inside an NMR sample cell to study proline isomerization in the pressure-sensitized L50A ubiquitin mutant. Whereas in two unfolded heptapeptides, X-Pro peptide bonds isomerized ca 1.6-fold faster at 1 bar than at 2.5 kbar, for ubiquitin ca eight-fold faster isomerization was observed for Pro-38 and ca t...
Source: Mol Biol Cell - April 25, 2024 Category: Molecular Biology Authors: Elahe Masoumzadeh Jinfa Ying James L Baber Philip Anfinrud Ad Bax Source Type: research
Cationic nanocarriers: A potential approach for targeting negatively charged cancer cell
Adv Colloid Interface Sci. 2024 Apr 22;327:103160. doi: 10.1016/j.cis.2024.103160. Online ahead of print.ABSTRACTCancer, a widespread and lethal disease, necessitates precise therapeutic interventions to mitigate its devastating impact. While conventional chemotherapy remains a cornerstone of cancer treatment, its lack of specificity towards cancer cells results in collateral damage to healthy tissues, leading to adverse effects. Thus, the quest for targeted strategies has emerged as a critical focus in cancer research. This review explores the development of innovative targeting methods utilizing novel drug delivery syste...
Source: Advances in Colloid and Interface Science - April 25, 2024 Category: Chemistry Authors: Monika Vishwakarma Poornima Agrawal Sakshi Soni Surbhi Tomar Tanweer Haider Sushil K Kashaw Vandana Soni Source Type: research
Exposure to hexafluoropropylene oxide trimer acid (HFPO-TA) impairs 5-HT metabolism by impacting the brain-gut axis in mice
Chemosphere. 2024 Apr 23:142035. doi: 10.1016/j.chemosphere.2024.142035. Online ahead of print.ABSTRACTHexafluoropropylene oxide trimer acid (HFPO-TA) has been found to cause hepatotoxicity, lipotoxicity, and cytotoxicity. However, the effects of HFPO-TA exposure on nervous system toxicity are still unclear. Here, six-week-old male C57BL/6J mice were treated with 2, 20, and 200 μg/L HFPO-TA for six weeks. The untargeted transcriptome analysis was employed to identify differentially expressed mRNAs in the tissue of mouse hippocampi. Then, the levels of neurotransmitters were detected by ELISA analysis in hippocampal and co...
Source: Chemosphere - April 25, 2024 Category: Chemistry Authors: Xiaoxian Xie Lei Sun Haoshen Xu Mengya Zhang Jiafeng Zhou Shulin Du Zhengwei Fu Zezhi Li Source Type: research
Construction of BiOCl/bismuth-based halide perovskite heterojunctions derived from the metal-organic framework CAU-17 for effective photocatalytic degradation
Chemosphere. 2024 Apr 23:142114. doi: 10.1016/j.chemosphere.2024.142114. Online ahead of print.ABSTRACTThe designed synthesis of an S-scheme heterojunction has possessed a great potential for improving photocatalytic wastewater treatment by demostrating increased the photoredox capacity and improved the charge separation efficiency. Here, we introduce the fabrication of a heterojunction-based photocatalyst comprising bismuth oxychloride (BiOCl) and bismuth-based halide perovskite (BHP) nanosheets, derived from metal-organic frameworks (MOFs). Our composite photocatalyst is synthesized through a one-pot solvothermal strateg...
Source: Chemosphere - April 25, 2024 Category: Chemistry Authors: Ai Le Hoang Pham Vinh Huu Nguyen Taeyoon Lee Van Cuong Nguyen Trinh Duy Nguyen Source Type: research
Attuning doped ZnO-based composites for an effective light-driven mineralization of pharmaceuticals via PMS activation
In this study, we investigated the effect of doping ZnO nanoparticles with either copper, gallium or indium on the structure, morphology, photophysical properties and photocatalytic mineralization of pharmaceuticals. Their architecture was further improved through the fabrication of composites, pairing the best performing doped ZnO with either BaFe12O19 or nickel nanoparticles. Their suitability was tested on a complex 60-ppm multi-pollutant solution (tetracycline, levofloxacin and lansoprazole). The activation strategy combined photocatalysis with peroxymonosulfate (PMS) as an environmentally friendly source of highly oxi...
Source: Chemosphere - April 25, 2024 Category: Chemistry Authors: Roger Bujald ón Majdi Benamara Ramzi Dhahri Elvira G ómez Albert Serr à Source Type: research
Alleviating binary toxicity of polystyrene nanoplastics and atrazine to Chlorella vulgaris through humic acid interaction: Long-term toxicity using environmentally relevant concentrations
In this study, microalgae Chlorella vulgaris (C. vulgaris) were simultaneously exposed to environmental concentrations of amino-functionalized polystyrene nanoplastics (PS-NH2; 0.05, 0.1, 0.2, 0.3 and 0.4 mg/L) and the world's second most used pesticide, the herbicide atrazine (ATZ; 10 μg/L), in the absence and presence of humic acid (HA; 1 mg/L) for 21 days. Due to the low concentrations of PS-NH2, the majority of them could not cause a significant difference in the end-points of biomass, chlorophylls a and b, total antioxidant, total protein, and superoxide dismutase and malondialdehyde compared to the control group (p&...
Source: Chemosphere - April 25, 2024 Category: Chemistry Authors: Mehdi Khoshnamvand Dongmei You Yafang Xie Yixiao Feng Marriya Sultan De-Sheng Pei Ailing Fu Source Type: research
Enhanced efficiency of AgAlO < sub > 2 < /sub > /g-C < sub > 3 < /sub > N < sub > 4 < /sub > binary composite to degrade organic pollutants for environmental remediation under visible light irradiation
This study explores the utilization of semiconductor-based photocatalysts for environmental remediation through photocatalytic degradation, harnessing solar energy for effective treatment. The primary focus is on the application of photocatalytic technology for the degradation of 2-chlorophenol and methylene blue, critical pollutants requiring remediation. The research involves the synthesis of binary AgAlO2/g-C3N4 nanocomposites through an exchange ion method, subsequent calcination, and sonication. This process enhances the transfer of photogenerated electrons from AgAlO2 to g-C3N4, resulting in a significantly increased...
Source: Chemosphere - April 25, 2024 Category: Chemistry Authors: Bobby Refokry Oeza Naveed Ahmad Kim Hoong Ng None Widyastuti Cheru Talbachew Haile Chung Feng Jeffrey Kuo Source Type: research
Sandwich-like voltametric immunosensing of interleukin-8 based on β-cyclodextrin/carbon nanotubes and methylthionine chloride@UIO-66 framework
Anal Sci. 2024 Apr 25. doi: 10.1007/s44211-024-00574-y. Online ahead of print.ABSTRACTThe level of interleukin-8 (IL-8) in the body is an effective factor for the early diagnosis of acute tubular necrosis and oral tumor. In this work, a novel sandwich-like voltametric immunosensor (SVS) of IL-8 was constructed by preparing β-cyclodextrin/carbon nanotube (CD/CNT) to immobilize primary antibody (PAb) of IL-8 and UIO-66-NH2 MOFs structure to immobilize second antibody (SAb) and methylene blue (Mb) probe. In this designed SVS, the prepared CD/CNT nanohybrid with large surface area and conductivity can immobilize PAb via simpl...
Source: Anal Sci - April 25, 2024 Category: Chemistry Authors: Zhihua Zhou Jiajia Lin Daoping Yue Huaiyu Chen Sheng Chen Source Type: research
High sensitivity atomic fluorescence spectroscopy for the detection of As < sup > III < /sup > by selective electrolysis of arsenic on nanoflowers-like Fe/NFE
This article attempts to achieve selective electroreduction of AsIII through the construction of electrode surfaces with different structures and materials from the perspective of interface reactions. Among the four transition metal modifiers, the iron modified nickel foam electrode with nano-flower structure documented higher efficiency in inducing arsenic reduction and better species selectivity. Systematic electrochemical and spectroscopic tests suggest that strong adsorption effect between Fe and AsIII, appropriate hydrogen evolution potential, and catalytic activity jointly promote efficient electroreduction of AsIII....
Source: Talanta - April 25, 2024 Category: Chemistry Authors: Ying-Ying Gao Xin-An Yang Wang-Bing Zhang Source Type: research
Rapid analysis and classification of blue ballpoint pen inks using high-performance thin-layer chromatography and digital color analysis
Talanta. 2024 Apr 16;275:126117. doi: 10.1016/j.talanta.2024.126117. Online ahead of print.ABSTRACTDespite the rise of digitalization, the scrutiny of paper documents with stamps and signatures remains crucial, constituting 55-60 % of document examination court cases. Developing accessible and robust ink analysis techniques is an ongoing challenge for forensic scientists. To tackle issues related to the identification and classification of dye components in blue ballpoint pens, we have proposed an advanced approach that integrates high-performance thin-layer chromatography (HPTLC), digital color analysis (DCA), and hierarc...
Source: Talanta - April 25, 2024 Category: Chemistry Authors: Anastasia V Kravchenko Daria V Makeeva Ilya Tumkin Andrey V Kalinichev Source Type: research
