Microwave-Assisted Synthesis of Highly Sulfated Mannuronate Glycans as Potential Inhibitors against SARS-CoV-2
Org. Biomol. Chem., 2024, Accepted Manuscript DOI: 10.1039/D4OB00466C, PaperYirong Zhu, Xiaotong Wang, Siqian Lu, Jibin Zheng, Youling Liang, Liangliang Zhang, Pengfei Fang, Peng Xu, Biao Yu, You Yang Algae-based marine carbohydrate drugs are typically decorated with negative ion groups such as carboxylation and sulfation. However, precise synthesis of highly sulfated alginates is challenging, thus impeding their structure –activity relationship... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic and Biomolecular Chemistry)
Source: RSC - Organic and Biomolecular Chemistry - April 25, 2024 Category: Molecular Biology Authors: Yirong Zhu Source Type: research
Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Endogenous Antimicrobial-Immunomodulatory Molecules: Networking Biomolecules of Innate Immunity
Chembiochem. 2024 Apr 24:e202400089. doi: 10.1002/cbic.202400089. Online ahead of print.ABSTRACTEndogenous antimicrobial-immunomodulatory molecules (EAIMs) are essential to immune-mediated human health and evolution. Conventionally, antimicrobial peptides (AMPs) have been regarded as the dominant endogenous antimicrobial molecule; however, AMPs are not sufficient to account for the full spectrum of antimicrobial-immunomodulatory duality occurring within the human body. The threat posed by pathogenic microbes is pervasive with the capacity for widespread impact across many organ systems and multiple biochemical pathways; ac...
Source: Chembiochem - April 24, 2024 Category: Biochemistry Authors: Donald F Weaver Source Type: research
Boosting one-step degradation of shrimp shell waste to produce chitin oligosaccharides at smart nanoscale enzyme reactor with liquid-solid system
Int J Biol Macromol. 2024 Apr 22:131787. doi: 10.1016/j.ijbiomac.2024.131787. Online ahead of print.ABSTRACTChitin oligosaccharides (CTOS) possess potential applications in food, medicine, and agriculture. However, lower mass transfer and catalytic efficiency are the main kinetic limitations for the production of CTOS from shrimp shell waste (SSW) and crystalline chitin. Chemical or physical methods are usually used for pretreatment to improve chitinase hydrolysis efficiency, but this is not eco-friendly and cost-effective. To address this challenge, a chitinase nanoreactor with the liquid-solid system (BcChiA1@ZIF-8) was ...
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Ziteng Wang Yijin Cai Mingxuan Li Xiaoru Wan Li Mi Wenge Yang Yonghong Hu Source Type: research
Sublethal acetamiprid affects reproduction, development and disrupts gene expression in Binodoxys communis
Environ Sci Pollut Res Int. 2024 Apr 24. doi: 10.1007/s11356-024-33415-6. Online ahead of print.ABSTRACTAt present, understanding of neonicotinoid toxicity in arthropods remains limited. We here evaluated the lethal and sublethal effects of acetamiprid in F0 and F1 generations of Binodoxys communis using a range of sublethal concentrations. The 10% lethal concentration (LC10) and half lethal concentration (LC25) of ACE had negative effects on the B. communis survival rate, adult longevity, parasitism rate, and emergence rate, and significantly prolonged the duration of the developmental cycle. ACE also had intergenerationa...
Source: Environmental Science and Pollution Research International - April 24, 2024 Category: Environmental Health Authors: Likang Zhao Hui Xue Punniyakotti Elumalai Xiangzhen Zhu Li Wang Kaixin Zhang Dongyang Li Jichao Ji Junyu Luo Jinjie Cui Xueke Gao Source Type: research
Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in haematococcus pluvialis
Bioresour Technol. 2024 Apr 22:130729. doi: 10.1016/j.biortech.2024.130729. Online ahead of print.ABSTRACTLow efficiency of the cultivation process is a major obstacle in the commercial production of Haematococcus pluvialis. Germination of red, non-motile cells is an efficient strategy for rapid acquisition of zoospores. However, the regulatory mechanisms associated with germination remain unexplored. In the present study, it was confirmed that the mitochondrial alternative oxidase (AOX) pathway accelerates H. pluvialis cell germination, and the regulatory mechanisms were clarified. When the AOX pathway was inhibited, the ...
Source: Bioresource Technology - April 24, 2024 Category: Biotechnology Authors: Jing Li Litao Zhang Wenjie Yu Mengjie Zhang Feng Chen Jianguo Liu Source Type: research
Advances in the understanding and exploitation of carbohydrate-active enzymes
Curr Opin Chem Biol. 2024 Apr 23;80:102457. doi: 10.1016/j.cbpa.2024.102457. Online ahead of print.ABSTRACTCarbohydrate-active enzymes (CAZymes) are responsible for the biosynthesis, modification and degradation of all glycans in Nature. Advances in genomic and metagenomic methodologies, in conjunction with lower cost gene synthesis, have provided access to a steady stream of new CAZymes with both well-established and novel mechanisms. At the same time, increasing access to cryo-EM has resulted in exciting new structures, particularly of transmembrane glycosyltransferases of various sorts. This improved understanding has r...
Source: Current Opinion in Chemical Biology - April 24, 2024 Category: Biochemistry Authors: Rajneesh K Bains Seyed Amirhossein Nasseri Jacob F Wardman Stephen G Withers Source Type: research
Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Advances in the understanding and exploitation of carbohydrate-active enzymes
Curr Opin Chem Biol. 2024 Apr 23;80:102457. doi: 10.1016/j.cbpa.2024.102457. Online ahead of print.ABSTRACTCarbohydrate-active enzymes (CAZymes) are responsible for the biosynthesis, modification and degradation of all glycans in Nature. Advances in genomic and metagenomic methodologies, in conjunction with lower cost gene synthesis, have provided access to a steady stream of new CAZymes with both well-established and novel mechanisms. At the same time, increasing access to cryo-EM has resulted in exciting new structures, particularly of transmembrane glycosyltransferases of various sorts. This improved understanding has r...
Source: Current Opinion in Chemical Biology - April 24, 2024 Category: Biochemistry Authors: Rajneesh K Bains Seyed Amirhossein Nasseri Jacob F Wardman Stephen G Withers Source Type: research
Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Poly(I:C)/c-GAMP/Alum ternary adjuvant system synergistically enhances the immunogenicity of glycopeptide antigen
(Source: Journal of Carbohydrate Chemistry)
Source: Journal of Carbohydrate Chemistry - April 24, 2024 Category: Chemistry Authors: Zhou, Shi-Hao Zou, Yong-Ke Liu, Yan-Ling Zhang, Ru-Yan Wen, Yu Ding, Dong Guo, Jun Source Type: research
Syntheses of propargyl glycosides of disaccharides of the capsular polysaccharides from serotypes 4, 7F, and 9V Streptococcus pneumoniae for glycoconjugate vaccines
(Source: Journal of Carbohydrate Chemistry)
Source: Journal of Carbohydrate Chemistry - April 24, 2024 Category: Chemistry Authors: Taylor, Seth A. White, Kendell Patton, Regan Linder, Anna Shin, Spencer Nelson, Sophie Haymore, Jared Zambrano, Joseph Hayden, Marcus Gubler, Shawn Meng, Tianyao Finn, M.G. Teyton, Luc Deng, Shenglou Savage, Paul B. Source Type: research
Cyclopropylmethylidene as a versatile protecting group for carbohydrate synthesis
(Source: Journal of Carbohydrate Chemistry)
Source: Journal of Carbohydrate Chemistry - April 24, 2024 Category: Chemistry Authors: Li, Xiaohua Ibrahim, Nasma Jaber, Zahraa Ali, Sara Masood, Isha Source Type: research
Approaches related to the synthesis of Fmoc-Ser/Thr[GalNAc(Ac)3- & #945;-D]-OH
(Source: Journal of Carbohydrate Chemistry)
Source: Journal of Carbohydrate Chemistry - April 24, 2024 Category: Chemistry Authors: Liu, Wenqiang He, Pan Shang, Shiying Tan, Zhongping Source Type: research
Monitoring carbohydrate 3D structure quality with the Privateer database
Abstract
The remediation of the carbohydrate data of the Protein Data Bank (PDB) has brought numerous enhancements to the findability and interpretability of deposited glycan structures, yet crucial quality indicators are either missing or hard to find on the PDB pages. Without a way to access wider glycochemical context, problematic structures may be taken as fact by keen but inexperienced scientists. The Privateer software is a validation and analysis tool that provides access to a number of metrics and links to external experimental resources, allowing users to evaluate structures using carbohydrate-specific methods. He...
Source: Beilstein Journal of Organic Chemistry - April 24, 2024 Category: Chemistry Authors: Beilstein Journal of Organic Chemistry - Syndicated Feeds Tags: carbohydrates database N-glycans N-glycosylation polysaccharides validation website Full Research Paper Source Type: research
