Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity
Mar Biotechnol (NY). 2024 Apr 26. doi: 10.1007/s10126-024-10311-1. Online ahead of print.ABSTRACTPolysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from Sargassum sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as Microbulbifer thermotolerans. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 ...
Source: Marine Biotechnology - April 26, 2024 Category: Biotechnology Authors: Xue Li Miao Yang Kunlian Mo Yonghua Hu Hanjie Gu Dongmei Sun Shixiang Bao Huiqin Huang Source Type: research
Biomass to green surfactants: Microwave-assisted transglycosylation of wheat bran for alkyl glycosides production
This study introduces microwave-assisted transglycosylation in transforming wheat bran, a substantial agricultural side stream, into these valuable compounds. Compared to traditional heating, microwave-assisted processing significantly enhances the product yield by 53 % while reducing the reaction time by 72 %, achieving a yield of 29 % within 5 h. This enhancement results from the microwave's capacity to activate intermolecular hydrogen and glycosidic bonds, thereby facilitating transglycosylation. Life-cycle assessment (LCA) and techno-economic analysis (TEA) demonstrate the benefits of microwave heating in reducing ener...
Source: Bioresource Technology - April 26, 2024 Category: Biotechnology Authors: Junyu Mi Jiong Cheng Kian Hong Ng Ning Yan Source Type: research
Sugar-coated survival: N-glycosylation as a unique bearded dragon venom resistance trait within Australian agamid lizards
This study zeroes in on the particular motifs within the orthosteric sites of post-synaptic nicotinic acetylcholine receptors (nAChR) that confer resistance to α-neurotoxins, often through structural alterations of nAChR. This research examined Australian agamid lizards, a primary prey group for Australian elapid snakes, which are subject to predatory selection pressures. We previously showed that Pogona vitticeps (Central bearded dragon) was resistant to α-neurotoxic snake venoms through a steric hindrance form resistance evolving within the nAChR orthosteric, specifically through the 187-189NVT motif resulting in the p...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - April 26, 2024 Category: Biochemistry Authors: Uthpala Chandrasekara Marco Mancuso Joanna Sumner Dan Edwards Christina N Zdenek Bryan G Fry Source Type: research
Diet-induced metabolic and immune impairments are sex-specifically modulated by soluble TNF signaling in the 5xFAD mouse model of Alzheimer's disease
Neurobiol Dis. 2024 Apr 24:106511. doi: 10.1016/j.nbd.2024.106511. Online ahead of print.ABSTRACTEmerging evidence indicates that high-fat, high carbohydrate diet (HFHC) impacts central pathological features of Alzheimer's disease (AD) across both human incidences and animal models. However, the mechanisms underlying this association are poorly understood. Here, we identify compartment-specific metabolic and inflammatory dysregulations that are induced by HFHC diet in the 5xFAD mouse model of AD pathology. We observe that both male and female 5xFAD mice display exacerbated adiposity, cholesterolemia, and dysregulated insul...
Source: Neurobiology of Disease - April 26, 2024 Category: Neurology Authors: Maria Elizabeth De Sousa Rodrigues MacKenzie L Bolen Lisa Blackmer-Raynolds Noah Schwartz Jianjun Chang Mal ú Gámez Tansey Timothy Robert Sampson Source Type: research
Regulation of sialic acid metabolism in cancer
Carbohydr Res. 2024 Apr 22;539:109123. doi: 10.1016/j.carres.2024.109123. Online ahead of print.ABSTRACTSialic acid, the terminal structure of cell surface glycans, has essential functions in regulating immune response, cell-to-cell communication, and cell adhesion. More importantly, an increased level of sialic acid, termed hypersialylation, has emerged as a commonly observed phenotype in cancer. Therefore, targeting sialic acid ligands (sialoglycans) and their receptors (Siglecs) may provide a new therapeutic approach for cancer immunotherapy. We highlight the complexity of the sialic acid metabolism and its involvement ...
Source: Carbohydrate Research - April 26, 2024 Category: Genetics & Stem Cells Authors: Filip Filipsky Heinz L äubli Source Type: research
A review on triterpenoid and triterpenoid saponins from Xanthoceras sorbifolium Bung
Carbohydr Res. 2024 Apr 23;539:109120. doi: 10.1016/j.carres.2024.109120. Online ahead of print.ABSTRACTXanthoceras sorbifolium Bunge, also known as Tu-Mu-Gua and Wen-Dan-Ge-Zi, has several applications. Clinical data and experimental studies have shown anti-tumor, anti-inflammatory, anti-bacterial, and anti-oxidant properties of Xanthoceras sorbifolium Bunge that inhibits prostate hyperplasia, lowers blood pressure and lipid level, and treats enuresis and urinary incontinence. It also has neuroprotective effects and can treat Alzheimer's disease and Parkinson's syndrome. The research on the chemical composition and pharma...
Source: Carbohydrate Research - April 26, 2024 Category: Genetics & Stem Cells Authors: Wen-Jin Cui Rui-Hong Li Xiao-Lan Chen Zi-Ming Xia Si-Fan Liu Min Li Li Chen Ying Tian Bin Li Guang-Jie Zhang Shu-Chen Liu Lin Wang Source Type: research
Reading and Writing the Human Glycocode
Annu Rev Biochem. 2024 Apr 26. doi: 10.1146/annurev-biochem-030122-044347. Online ahead of print.ABSTRACTThe complex carbohydrate structures decorating human proteins and lipids, also called glycans, are abundantly present at cell surfaces and in the secretome. Glycosylation is vital for biological processes including cell-cell recognition, immune responses, and signaling pathways. Therefore, the structural and functional characterization of the human glycome is gaining more and more interest in basic biochemistry research and in the context of developing new therapies, diagnostic tools, and biotechnology applications. For...
Source: Annual Review of Biochemistry - April 26, 2024 Category: Biochemistry Authors: Noortje de Haan Mathias I Nielsen Hans H Wandall Source Type: research
Synthesis of C3- < em > epi < /em > -virenose and anomerically activated derivatives
Tetrahedron Lett. 2024 Apr;140:155041. doi: 10.1016/j.tetlet.2024.155041.ABSTRACTA 9-step synthetic route to a protected form of the C3-epimer of virenose from D-fucose is described. C3-epi-virenose is the carbohydrate unit of the bioactive polyketide elsamicin B and part of the carbohydrate unit of elsamicin A. The developed route enabled preparation of anomerically activated forms of this unique C6-deoxy sugar, including derivatives with 1-acetyl, 1-acetylthio, 1-trichloroacetimidate, 1-bromo, and 1-fluoro substituents.PMID:38665383 | PMC:PMC7615872 | DOI:10.1016/j.tetlet.2024.155041 (Source: Tetrahedron Letters)
Source: Tetrahedron Letters - April 26, 2024 Category: Chemistry Authors: Liesa R öder Sofia Torres Venegas Klaus Wurst Thomas Magauer Source Type: research
Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity
Mar Biotechnol (NY). 2024 Apr 26. doi: 10.1007/s10126-024-10311-1. Online ahead of print.ABSTRACTPolysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from Sargassum sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as Microbulbifer thermotolerans. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 ...
Source: Marine Biotechnology - April 26, 2024 Category: Biotechnology Authors: Xue Li Miao Yang Kunlian Mo Yonghua Hu Hanjie Gu Dongmei Sun Shixiang Bao Huiqin Huang Source Type: research
Biomass to green surfactants: Microwave-assisted transglycosylation of wheat bran for alkyl glycosides production
This study introduces microwave-assisted transglycosylation in transforming wheat bran, a substantial agricultural side stream, into these valuable compounds. Compared to traditional heating, microwave-assisted processing significantly enhances the product yield by 53 % while reducing the reaction time by 72 %, achieving a yield of 29 % within 5 h. This enhancement results from the microwave's capacity to activate intermolecular hydrogen and glycosidic bonds, thereby facilitating transglycosylation. Life-cycle assessment (LCA) and techno-economic analysis (TEA) demonstrate the benefits of microwave heating in reducing ener...
Source: Bioresource Technology - April 26, 2024 Category: Biotechnology Authors: Junyu Mi Jiong Cheng Kian Hong Ng Ning Yan Source Type: research
Sugar-coated survival: N-glycosylation as a unique bearded dragon venom resistance trait within Australian agamid lizards
This study zeroes in on the particular motifs within the orthosteric sites of post-synaptic nicotinic acetylcholine receptors (nAChR) that confer resistance to α-neurotoxins, often through structural alterations of nAChR. This research examined Australian agamid lizards, a primary prey group for Australian elapid snakes, which are subject to predatory selection pressures. We previously showed that Pogona vitticeps (Central bearded dragon) was resistant to α-neurotoxic snake venoms through a steric hindrance form resistance evolving within the nAChR orthosteric, specifically through the 187-189NVT motif resulting in the p...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - April 26, 2024 Category: Biochemistry Authors: Uthpala Chandrasekara Marco Mancuso Joanna Sumner Dan Edwards Christina N Zdenek Bryan G Fry Source Type: research
Regulation of sialic acid metabolism in cancer
Carbohydr Res. 2024 Apr 22;539:109123. doi: 10.1016/j.carres.2024.109123. Online ahead of print.ABSTRACTSialic acid, the terminal structure of cell surface glycans, has essential functions in regulating immune response, cell-to-cell communication, and cell adhesion. More importantly, an increased level of sialic acid, termed hypersialylation, has emerged as a commonly observed phenotype in cancer. Therefore, targeting sialic acid ligands (sialoglycans) and their receptors (Siglecs) may provide a new therapeutic approach for cancer immunotherapy. We highlight the complexity of the sialic acid metabolism and its involvement ...
Source: Carbohydrate Research - April 26, 2024 Category: Genetics & Stem Cells Authors: Filip Filipsky Heinz L äubli Source Type: research
A review on triterpenoid and triterpenoid saponins from Xanthoceras sorbifolium Bung
Carbohydr Res. 2024 Apr 23;539:109120. doi: 10.1016/j.carres.2024.109120. Online ahead of print.ABSTRACTXanthoceras sorbifolium Bunge, also known as Tu-Mu-Gua and Wen-Dan-Ge-Zi, has several applications. Clinical data and experimental studies have shown anti-tumor, anti-inflammatory, anti-bacterial, and anti-oxidant properties of Xanthoceras sorbifolium Bunge that inhibits prostate hyperplasia, lowers blood pressure and lipid level, and treats enuresis and urinary incontinence. It also has neuroprotective effects and can treat Alzheimer's disease and Parkinson's syndrome. The research on the chemical composition and pharma...
Source: Carbohydrate Research - April 26, 2024 Category: Genetics & Stem Cells Authors: Wen-Jin Cui Rui-Hong Li Xiao-Lan Chen Zi-Ming Xia Si-Fan Liu Min Li Li Chen Ying Tian Bin Li Guang-Jie Zhang Shu-Chen Liu Lin Wang Source Type: research
Reading and Writing the Human Glycocode
Annu Rev Biochem. 2024 Apr 26. doi: 10.1146/annurev-biochem-030122-044347. Online ahead of print.ABSTRACTThe complex carbohydrate structures decorating human proteins and lipids, also called glycans, are abundantly present at cell surfaces and in the secretome. Glycosylation is vital for biological processes including cell-cell recognition, immune responses, and signaling pathways. Therefore, the structural and functional characterization of the human glycome is gaining more and more interest in basic biochemistry research and in the context of developing new therapies, diagnostic tools, and biotechnology applications. For...
Source: Annual Review of Biochemistry - April 26, 2024 Category: Biochemistry Authors: Noortje de Haan Mathias I Nielsen Hans H Wandall Source Type: research
Synthesis of C3- < em > epi < /em > -virenose and anomerically activated derivatives
Tetrahedron Lett. 2024 Apr;140:155041. doi: 10.1016/j.tetlet.2024.155041.ABSTRACTA 9-step synthetic route to a protected form of the C3-epimer of virenose from D-fucose is described. C3-epi-virenose is the carbohydrate unit of the bioactive polyketide elsamicin B and part of the carbohydrate unit of elsamicin A. The developed route enabled preparation of anomerically activated forms of this unique C6-deoxy sugar, including derivatives with 1-acetyl, 1-acetylthio, 1-trichloroacetimidate, 1-bromo, and 1-fluoro substituents.PMID:38665383 | PMC:PMC7615872 | DOI:10.1016/j.tetlet.2024.155041 (Source: Tetrahedron Letters)
Source: Tetrahedron Letters - April 26, 2024 Category: Chemistry Authors: Liesa R öder Sofia Torres Venegas Klaus Wurst Thomas Magauer Source Type: research
