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Therapy: Gene Therapy

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Total 490 results found since Jan 2013.

Potential of siRNA-albumin complex against cancer.
Abstract RNA interference is a highly specific as well as efficient technology for gene therapy application in molecular oncology. The present study was planned to develop an efficient and stable tumor selective delivery mechanism for siRNA gene therapy for the purpose of both diagnosis as well as therapy. We have used 20 Male wistar rats for the formation of colon cancer model and utilized albumin as carrier molecule for the delivery of siRNA against vascular endothelial growth factor receptor 2 (VEGF R2). The study results confirmed efficient delivery of siRNA at tumor site as confirmed by tagging of siRNA-album...
Source: Chemico-Biological Interactions - April 27, 2018 Category: Molecular Biology Authors: Liu N, Qi YH, Cheng CT, Yang WB, Malhotra A, Zhou Q Tags: Chem Biol Interact Source Type: research

AKT2 siRNA delivery with amphiphilic-based polymeric micelles show efficacy against cancer stem cells.
Authors: Rafael D, Gener P, Andrade F, Seras-Franzoso J, Montero S, Fernández Y, Hidalgo M, Arango D, Sayós J, Florindo HF, Abasolo I, Schwartz S, Videira M Abstract Development of RNA interference-based therapies with appropriate therapeutic window remains a challenge for advanced cancers. Because cancer stem cells (CSC) are responsible of sustaining the metastatic spread of the disease to distal organs and the progressive gain of resistance of advanced cancers, new anticancer therapies should be validated specifically for this subpopulation of cells. A new amphihilic-based gene delivery system that combines Plu...
Source: Drug Delivery - April 20, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

812 Type VII collagen and Nesprin 2, LINCing the basement membrane to altered cell cycle and increased DNA damage
Epidermolysis Bullosa is a severe blistering disease caused by mutations in basement membrane genes and is characterised by severe blistering of the skin and increased predisposition to cancer. To investigate the role of the skin basement membrane, siRNA knockdown of type IV, VII and XVII collagens was performed in primary keratinocytes. Global transcriptomic analysis was carried out using RNA-Seq. Dysregulation of genes involved in DNA damage and cell cycle control was seen in cells with Col7 and Col17 knockdown.
Source: Journal of Investigative Dermatology - April 19, 2018 Category: Dermatology Authors: S. Marsh, M.P. Caley, V. Martins, M. Chen, J. McGrath, M. Barnes, E.A. O'Toole Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research

761 Topically-delivered gene suppressing nanocontruct targets IL-17RA for psoriasis
Activated interleukin (IL)-23/Th17 signaling is critical for psoriasis pathogenesis, and systemic IL-17 inhibitors are highly effective therapy. However, most patients have mild to moderate disease and no targeted topical therapeutic option. We developed a topically-delivered gene regulating nanoconstruct, comprised of antisense DNA or siRNA in a dense spherical configuration. These spherical nucleic acids or SNAs penetrate the skin barrier to knock down cutaneous gene targets. We generated anti-human and anti-mouse liposomal DNA SNAs targeting the IL-17A receptor (ILRA) and tested their efficacy in reducing IL17RA and imp...
Source: Journal of Investigative Dermatology - April 19, 2018 Category: Dermatology Authors: H. Liu, R. Kang, K. Bagnowski, J. Yu, X. Cheng, S. Radecki, W. Daniel, D. Giljohann, A.S. Paller Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research

Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery
Publication date: Available online 21 February 2018 Source:Asian Journal of Pharmaceutical Sciences Author(s): Anna Slita, Anna Egorova, Eudald Casals, Anton Kiselev, Jessica M. Rosenholm Gene therapy using siRNA molecules is nowadays considered as a promising approach. For successful therapy, development of a stable and reliable vector for siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles (MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid carrying capacity. To overcome this hurdle, we here modified t...
Source: Asian Journal of Pharmaceutical Sciences - April 14, 2018 Category: Drugs & Pharmacology Source Type: research

A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy
Chem. Commun., 2018, Advance Article DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shu-Meng Bai, Chun-Hua Lu, Huang-Hao Yang A new siRNA delivery platform based on black phosphorus nanosheets is constructed which shows excellent therapy efficiency for tumors. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 12, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research

A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy.
Abstract Gene therapy with small interfering RNA (siRNA) has been proved to be a promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery system that has high efficiency in transporting siRNA without obvious side effects remains a challenge. Herein, we designed a new survivin siRNA delivery system based on polyethyleneimine functionalized black phosphorus (BP) nanosheets which could suppress tumor growth by silencing survivin expression. Combined with the photothermal properties of the BP nanosheets, the presented delivery system shows excellent ...
Source: Chemical Communications - March 12, 2018 Category: Chemistry Authors: Wang H, Zhong L, Liu Y, Xu X, Xing C, Wang M, Bai SM, Lu CH, Yang HH Tags: Chem Commun (Camb) Source Type: research

Interfering RNA with multi-targets for efficient gene suppression in HCC cells.
Abstract RNA interference (RNAi) technology has been widely used in therapeutics development, especially multiple targeted RNAi strategy, which is a better method for multiple gene suppression. In the study, interfering RNAs (iRNAs) were designed for carrying two or three different siRNA sequences in different secondary structure formats (loop or cloverleaf). By using these types of iRNAs, co-inhibition of survivin and B-cell lymphoma-2 (Bcl-2) was investigated in hepatocellular carcinoma (HCC) cells, and we obtained promising gene silencing effects without showing undesirable interferon response. Furthermore, su...
Source: International Journal of Molecular Medicine - March 9, 2018 Category: Molecular Biology Authors: Li T, Zhu YY, Ji Y, Zhou S Tags: Int J Mol Med Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specifi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - March 6, 2018 Category: Biochemistry Source Type: research

Black Phosphorus Nanosheets-Based siRNA Delivery System for Synergistic Photothermal and Gene Therapy
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shumeng Bai, Chun-Hua Lu, Huanghao Yang Gene therapy with small interfering RNA (siRNA) has been proved to be the promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 2, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research

Ruthenium dendrimers as carriers for anticancer siRNA.
This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Michlewska S, Ionov M, Maroto-Díaz M, Szwed A, Ihnatsyeu-Kachan A, Loznikova S, Shcharbin D, Maly M, Ramirez RG, de la Mata FJ, Bryszewska M Tags: J Inorg Biochem Source Type: research

Anti-apoptosis Effect of Decoy Receptor 3 in Cholangiocarcinoma Cell Line TFK-1.
CONCLUSIONS: The effect of DcR3 on the growth and apoptosis of cholangiocarcinoma has been demonstrated. DcR3 is not only a predictive marker for malignant tumor but it is also likely to be a potential target for cancer gene therapy. Further studies should focus on exploring the binding ligand of DcR3, the signaling pathway involved, and the molecular mechanism for the regulation of DcR3 expression in cholangiocarcinoma. PMID: 29271385 [PubMed - in process]
Source: Chinese Medical Journal - December 24, 2017 Category: General Medicine Authors: Xu YC, Cui J, Zhang LJ, Zhang DX, Xing BC, Huang XW, Wu JX, Liang CJ, Li GM Tags: Chin Med J (Engl) Source Type: research

Silencing of Phospholipase C gamma 2 Promotes Proliferation of Rat Hepatocytes In Vitro
In conclusion, these findings suggest that interference with PLCγ2 expression could relieve the inhibitory effect of PLCγ2 on hepaocyte apoptosis, thus promote proliferation through inactivating PKCδ‐mediated JNK1/2 signaling pathway. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Xiaoguang Chen, Xuemin Zhu, Yumei Liu, Qiongxia Lv, Jun Ma Tags: Article Source Type: research

Self ‐Assembled Aptamer‐Nanomedicine for Targeted Chemotherapy and Gene Therapy
In this study, a multifunctional aptamer‐nanomedicine (Apt‐NMed) achieving targeted chemotherapy and gene therapy of ALCL is developed. Apt‐NMed is formulated by self‐assembly of synthetic oligonucleotides containing CD30‐specific aptamer and anaplastic lymphoma kinase (ALK)‐specific siRNA followed by self‐loading of the chemotherapeutic drug doxorubicin (DOX). Apt‐NMed exhibits a well‐defined nanostructure (diameter 59 mm) and stability in human serum. Under aptamer guidance, Apt‐NMed specifically binds and internalizes targeted ALCL cells. Intracellular delivery of Apt‐NMed triggers rapid DOX releas...
Source: Small - December 4, 2017 Category: Nanotechnology Authors: Nianxi Zhao, Zihua Zeng, Youli Zu Tags: Full Paper Source Type: research