Characterization of the interaction of the antifungal and cytotoxic cyclic glycolipopeptide hassallidin with sterol-containing lipid membranes
This study demonstrates the mechanism of action of the natural compound hassallidin, and gives further insight into how bioactive lipopeptide metabolites selectively target eukaryotic cell membranes.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 19, 2019 Category: Biochemistry Source Type: research

Membrane fusogenic high-density lipoprotein nanoparticles
Publication date: Available online 15 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Hyungjin Kim, Tomohiro Nobeyama, Shinnosuke Honda, Kaori Yasuda, Nobuhiro Morone, Tatsuya MurakamiAbstractMembrane fusion under mildly acidic pH occurs naturally during viral infection in cells and has been exploited in the field of nanoparticle-mediated drug delivery to circumvent endosomal entrapment of the cargo. Herein, we aimed to confer virus-like fusogenic activity to HDL in the form of a ca. 10-nm disc comprising a discoidal lipid bilayer and two copies of a lipid-binding protein at the edge. A series...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 15, 2019 Category: Biochemistry Source Type: research

On the existence of endocytosis driven by membrane phase separations
We describe here that endocytosis driven by membrane phase transitions is regulated by the same signaling mechanisms that regulate macropinocytosis, and it may play diverse roles in cells from nutrient assimilation to membrane recycling, cell migration, and the initiation of quiescent or hibernating cell states. Membrane ordering and phase separations have been shown to promote endocytosis in diverse cell types, including fibroblasts, myocytes, glial cells, and immune cells. We propose that clathrin/dynamin-independent endocytosis represents a continuum of related mechanisms with variable but universal dependence on membra...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 13, 2019 Category: Biochemistry Source Type: research

Interactions of cholesterol and 7‑ketocholesterol with unsaturated fatty acids of different unsaturation degree – The monolayer study
Publication date: 1 August 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 8Author(s): J.L. Fidalgo Rodríguez, P. Dynarowicz-Latka, J. Minones CondeAbstractUnsaturated fatty acids (UFAs) are known to lower the level of sterols in blood, which accounts for their cardioprotective effect. To understand the molecular basis of this effect, Langmuir monolayer studies have been performed. A series of UFAs differing in the length of the fatty acid chain and the number of double bonds (oleic acid, OA; linoleic acid, LA; stearidonic acid, SDA; eicosanoic acid, EA) were mixed with cholesterol...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 13, 2019 Category: Biochemistry Source Type: research

Evidences of plasma membrane-mediated ROS generation upon ELF exposure in neuroblastoma cells
Publication date: Available online 11 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Caterina Merla, Micaela Liberti, Claudia Consales, Agnese Denzi, Francesca Apollonio, Carmela Marino, Barbara BenassiAbstractBackgroundMolecular mechanisms of interaction between cells and extremely low frequency magnetic fields (ELF-MFs) still represent a matter of scientific debate. In this paper, to identify the possible primary source of oxidative stress induced by ELF-MF in SH-SY5Y human neuroblastoma cells, we estimated the induced electric field and current density at the cell level.MethodsWe followed ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 12, 2019 Category: Biochemistry Source Type: research

A time domain study of surfactin penetrating a phospholipid monolayer at the air-water Interface investigated using sum frequency generation spectroscopy, infrared reflection absorption spectroscopy, and AFM-nano infrared microscopy
Publication date: Available online 12 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): S.A. Goussous, A.P. Fellows, M.T.L. Casford, P.B. DaviesAbstractWe have investigated the interaction of surfactin with a monolayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at the air-water interface as a function of time, following its injection into the sub-phase, using non-linear Sum Frequency Generation (SFG) vibrational spectroscopy and Infrared Reflection Absorption Spectroscopy (IRRAS). SFG resonances from the phospholipid and from the surfactin were distinguished from each other by using s...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 12, 2019 Category: Biochemistry Source Type: research

The structural arrangement and dynamics of the heteromeric GluK2/GluK5 kainate receptor as determined by smFRET
This article is part of a Special Issue entitled: Molecular biophysics of membranes and membrane proteins.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 12, 2019 Category: Biochemistry Source Type: research

Molecular insights on the interference of simplified lung surfactant models by gold nanoparticle pollutants
Publication date: Available online 10 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Sheikh I. Hossain, Neha S. Gandhi, Zak E. Hughes, Y.T. Gu, Suvash C. SahaAbstractInhaled nanoparticles (NPs) are experienced by the first biological barrier inside the alveolus known as lung surfactant (LS), a surface tension reducing agent, consisting of phospholipids and proteins in the form of the monolayer at the air-water interface. The monolayer surface tension is continuously regulated by the alveolus compression and expansion and protects the alveoli from collapsing. Inhaled NPs can reach deep into th...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 10, 2019 Category: Biochemistry Source Type: research

Structurally related glucosylated liposomes: Correlation of physicochemical and biological features
Publication date: Available online 10 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Alessandro Mauceri, Luisa Giansanti, Giuseppina Bozzuto, Maria Condello, Agnese Molinari, Luciano Galantini, Antonella Piozzi, Giovanna ManciniAbstractLiposomes functionalized on their surface with carbohydrates (glycoliposomes) represent an optimal approach for targeting of drugs to diseased tissues in vivo, thanks to biocompatibility, low toxicity and easy manufacturing of these lipid nanoparticles. Here we report on the study of liposomes including a novel glycosylated amphiphile and on the comparison of t...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 10, 2019 Category: Biochemistry Source Type: research

Interactons of cholesterol and 7‑ketocholesterol with unsaturated fatty acids of different unsaturation degree – The monolayer study
Publication date: Available online 9 June 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): J.L. Fidalgo Rodríguez, P. Dynarowicz-Latka, J. Miñones CondeAbstractUnsaturated fatty acids (UFAs) are known to lower the level of sterols in blood, which accounts for their cardioprotective effect. To understand the molecular basis of this effect, Langmuir monolayer studies have been performed. A series of UFAs differing in the length of the fatty acid chain and the number of double bonds (oleic acid, OA; linoleic acid, LA; stearidonic acid, SDA; eicosanoic acid, EA) were mixed with cholesterol...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 9, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 July 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 7Author(s): (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - June 9, 2019 Category: Biochemistry Source Type: research

The distal cytoplasmic tail of the influenza A M2 protein dynamically extends from the membrane
Publication date: Available online 30 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Grace Kim, Hayley E. Raymond, Alice L. Herneisen, Abigail Wong-Rolle, Kathleen P. HowardAbstractThe influenza A M2 protein is a multifunctional membrane-associated homotetramer that orchestrates several essential events in the viral infection cycle. The monomeric subunits of the M2 homotetramer consist of an N-terminal ectodomain, a transmembrane domain, and a C-terminal cytoplasmic domain. The transmembrane domain forms a four-helix proton channel that promotes uncoating of virions upon host cell entry. The m...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 31, 2019 Category: Biochemistry Source Type: research

Antimicrobial activity of amphiphilic neamine derivatives: Understanding the mechanism of action on Gram-positive bacteria
In this study, we have assessed its mechanism of action against Gram-positive bacteria, S. aureus and B. subtilis. By time killing curves, we assessed the bactericidal effect induced by 3′,6-dinonyl neamine on S. aureus MSSA and MRSA. By measuring the displacement of BODIPY™-TR cadaverine bound to lipoteichoic acids (LTA), we showed that 3′,6-dinonyl neamine interacts with these bacterial surface components. We also highlighted the ability of 3′,6-dinonyl neamine to enhance membrane depolarization and induce membrane permeability, by using fluorescent probes, DiSC3C(5) and propidium iodide, respecti...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 31, 2019 Category: Biochemistry Source Type: research

Small-angle neutron and X-ray scattering analysis of the supramolecular organization of rhodopsin in photoreceptor membranes
Publication date: Available online 30 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Tatiana B. Feldman, Oleksandr I. Ivankov, Alexander I. Kuklin, Tatiana N. Murugova, Marina A. Yakovleva, Olga A. Smitienko, Irina B. Kolchugina, Adam Round, Valentin I. Gordeliy, Alexander V. Belushkin, Mikhail A. OstrovskyAbstractThe supramolecular organization of the visual pigment rhodopsin in the photoreceptor membrane remains contentious. Specifically, whether this G protein-coupled receptor functions as a monomer or dimer remains unknown, as does the presence or absence of ordered packing of rhodopsin mo...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 30, 2019 Category: Biochemistry Source Type: research

Alterations to plasma membrane lipid contents affect the biophysical properties of erythrocytes from individuals with hypertension
This study reveals that alterations to the lipid contents of erythrocyte plasma membranes occur in hypertension, and these alterations in lipid composition result in morphological and physiological abnormalities that modify the dynamic properties of erythrocytes and contribute to the pathophysiology of hypertension.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 30, 2019 Category: Biochemistry Source Type: research

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis
Publication date: Available online 28 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Naomi L. Pollock, Megha Rai, Kailene S. Simon, Sophie J. Hesketh, Alvin C.K. Teo, Mayuriben Parmar, Pooja Sridhar, Richard Collins, Sarah C. Lee, Zoe N. Stroud, Saskia E. Bakker, Stephen P. Muench, C. Howard Barton, Gregory Hurlbut, David I. Roper, Corinne J.I. Smith, Timothy J. Knowles, Corinne M. Spickett, J. Malcolm East, Vincent PostisAbstractMost membrane proteins function through interactions with other proteins in the phospholipid bilayer, the cytosol or the extracellular milieu. Understanding the molec...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 30, 2019 Category: Biochemistry Source Type: research

The direct permeation of nanoparticles through the plasma membrane transiently modifies its properties
Publication date: Available online 28 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Daniele Zanella, Elena Bossi, Rosalba Gornati, Nuno Faria, Jonathan Powell, Giovanni BernardiniAbstractThe exposure to metal nanoparticles (NPs) has increased with their widespread use in industry, research and medicine. It is well known that NPs may enter cells and that this mechanism is crucial to exert both the therapeutic and toxicity effects. The main cellular entrance route is endocytosis-based, however, recent experimental studies, have reported that NPs can also enter the cell crossing directly the pla...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 30, 2019 Category: Biochemistry Source Type: research

Influence of levofloxacin and clarithromycin on the structure of DPPC monolayers
Publication date: Available online 28 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Stephanie Ortiz-Collazos, Paulo H.S. Picciani, Osvaldo N. Oliveira, Andre S. Pimentel, Karen J. EdlerAbstractResearch on lipid/drug interactions at the nanoscale underpins the emergence of synergistic mechanisms for topical drug administration. The structural understanding of bio-mimetic systems employing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as a lung surfactant model mixed with antibiotics, as well as their biophysical properties, is of critical importance to modulate the effectiveness of therap...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 28, 2019 Category: Biochemistry Source Type: research

Organization and dynamics of NBD-labeled lipids in lipid bilayer analyzed by FRET using the small membrane fluorescent probe AHBA as donor
Publication date: Available online 25 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Cássia Alessandra Marquezin, Amando Siuiti Ito, Eduardo Sérgio de SouzaAbstractFluorescent probes are employed to investigate natural and model membranes. It is important to know probe location and extent of perturbations they cause into the lipid bilayer. Förster Resonance Energy Transfer (FRET) is a useful tool to investigate phenomena involving plasma membranes, and reports in literature used relatively large fluorophores like 1,6-diphenylhexatriene, located at the center of the hydrophob...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 27, 2019 Category: Biochemistry Source Type: research

Resistance of early stationary phase E. coli to membrane permeabilization by the antimicrobial peptide Cecropin A
Publication date: Available online 23 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Anurag Agrawal, Nambirajan Rangarajan, James C. WeisshaarAbstractAntimicrobial peptides (AMPs) cause bacterial membrane permeabilization and ultimately cell death at low μM concentrations. The membrane permeabilization action of a moth derived AMP Cecropin A on E. coli cells in exponential growth (mid-log phase) is well studied. At 1× MIC concentration, Cecropin A penetrates the lipopolysaccharide (LPS) barrier and causes outer membrane (OM) and cytoplasmic membrane (CM) permeabilization. For non-sept...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 25, 2019 Category: Biochemistry Source Type: research

Interaction of Trastuzumab with biomembrane models at air-water interfaces mimicking cancer cell surfaces
Publication date: Available online 23 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Andrei Sakai, Ana Paula de Sousa Mesquista, Erica Valadares de Castro Levatti, Anita Hilda Straus, Helena B. Nader, Carla Cristina Lopes, Luciano CaseliAbstractTrastuzumab (Tmab) is a monoclonal antibody administered as targeted therapy for HER2-positive breast cancer whose molecular interactions at the HER2 receptor microenvironment are not completely clarified yet. This paper describes the influence of Tmab in the molecular organization of films of biological-relevant molecules at the air water interface. Fo...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 25, 2019 Category: Biochemistry Source Type: research

The Parkinson-associated human P5B-ATPase ATP13A2 modifies lipid homeostasis
Publication date: Available online 24 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Alejandra Lucía Marcos, Gerardo Raul Corradi, Luciana Romina Mazzitelli, Cecilia Irene Casali, María del Carmen Fernández Tome, Hugo Pedro Adamo, Felicitas de Tezanos PintoAbstractMutations in the ATP13A2 gene (PARK9, CLN12, OMIM 610513) were initially associated with a form of Parkinson's Disease (PD) known as Kufor Rakeb Syndrome (KRS). However, the genetic spectrum of ATP13A2-associated disorders was expanded in the last years, because it has been found to underlay variants of neuronal ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 25, 2019 Category: Biochemistry Source Type: research

Deciphering the potential involvement of PXMP2 and PEX11B in hydrogen peroxide permeation across the peroxisomal membrane reveals a role for PEX11B in protein sorting
Publication date: Available online 24 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Celien Lismont, Janet Koster, Sarah Provost, Myriam Baes, Paul P. Van Veldhoven, Hans R. Waterham, Marc FransenAbstractPeroxisomes have the intrinsic ability to produce and scavenge hydrogen peroxide (H2O2), a diffusible second messenger that controls diverse cellular processes by modulating protein activity through cysteine oxidation. Current evidence indicates that H2O2, a molecule whose physicochemical properties are similar to those of water, traverses cellular membranes through specific aquaporin channels...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 25, 2019 Category: Biochemistry Source Type: research

BamA is a pivotal protein in cell envelope synthesis and cell division in Deinococcus radiodurans
Publication date: Available online 16 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Jiangliu Yu, Luchun LuAbstractThe beta-barrel assembly machinery (BAM) is an indispensable complex for protein transportation located at the outer membrane of bacteria. BAM is composed of five subunits (BamA-E) in the model bacterium Escherichia coli. DR_0379 is a BamA homolog in Deinococcus radiodurans, but the other subunits have not been detected in this species. In the present study, deletion of bamA resulted in decreased growth rate and altered morphology of D. radiodurans. ΔbamA cells underwent abn...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 18, 2019 Category: Biochemistry Source Type: research

Synergistic and antibiofilm activity of the antimicrobial peptide P5 against carbapenem-resistant Pseudomonas aeruginosa
Publication date: Available online 13 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Melina Martinez, Sónia Gonçalves, Mário R. Felício, Patricia Maturana, Nuno C. Santos, Liliana Semorile, Axel Hollmann, Paulo C. MaffíaAbstractIn the search for new antimicrobial molecules, antimicrobial peptides (AMPs) offer a viable alternative to conventional antibiotics, as they physically disrupt the bacterial membranes, leading to membrane disruption and eventually cell death. In particular, the group of linear α-helical cationic peptides has attracted increasing ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 15, 2019 Category: Biochemistry Source Type: research

Loss of carotenoids from membranes of Pantoea sp. YR343 results in altered lipid composition and changes in membrane biophysical properties
Publication date: Available online 14 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Sushmitha Vijaya Kumar, Graham Taylor, Sahar Hasim, C. Patrick Collier, Abigail T. Farmer, Shawn R. Campagna, Amber N. Bible, Mitchel J. Doktycz, Jennifer Morrell-FalveyAbstractBacterial membranes are complex mixtures of lipids and proteins, the combination of which confers biophysical properties that allows cells to respond to environmental conditions. Carotenoids are sterol analogs that are important for regulating membrane dynamics. The membrane of Pantoea sp. YR343 is characterized by the presence of the c...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 15, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 June 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 6Author(s): (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 15, 2019 Category: Biochemistry Source Type: research

Phospholipid effects on SGLT1-mediated glucose transport in rabbit ileum brush border membrane vesicles
In conclusion, sodium-dependent glucose transport of rabbit ileal BBMV is modulated by (i) altered membrane surface charge, (ii) length of acyl chains via membrane thickness, and (iii) saturation of PL acyl chains altering membrane fluidity. Transport was attenuated by charged PL with longer and unsaturated acyl residues. Alterations of PL may provide a principle for attenuating dietary glucose uptake.Graphical abstractNa+-dependent glucose transport into rabbit ileal brush border membrane vesicles is inhibited by phospholipids (i) carrying an acidic head group, (ii) by longer acyl chains which increase membrane thickness ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 11, 2019 Category: Biochemistry Source Type: research

Surface interactions determined by stereostructure on the example of 7-hydroxycholesterol epimers – The Langmuir monolayer study
Publication date: Available online 8 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Anna Chachaj-Brekiesz, Anita Wnętrzak, Ewelina Lipiec, Patrycja Dynarowicz-LatkaAbstractStereoselective interactions are pivotal for molecular recognition between biomolecules and lipid surfaces. The aim of this study was to determine factors differencing molecular interactions between 7-hydroxycholesterol epimers (oxysterols, which excessively appear in pathological processes in human body) and natural membrane phospholipids. Two-component systems of different mutual proportions of 7-hydroxycholesterol (7&alp...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 8, 2019 Category: Biochemistry Source Type: research

Molecular basis for the different interactions of congeneric substrates with the polyspecific transporter AcrB
Publication date: Available online 8 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ivana Malvacio, Rosa Buonfiglio, Noemi D'Atanasio, Giovanni Serra, Andrea Bosin, Francesco Paolo Di Giorgio, Paolo Ruggerone, Rosella Ombrato, Attilio Vittorio VargiuAbstractThe drug/proton antiporter AcrB, which is part of the major efflux pump AcrABZ-TolC in Escherichia coli, is the most well-studied, paradigm transporter of the resistance nodulation-cell division (RND) superfamily. Despite the impressive ability of AcrB to transport many chemically unrelated compounds, only a few of these compounds have been...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 8, 2019 Category: Biochemistry Source Type: research

Activity and characterization of a pH-sensitive antimicrobial peptide
Publication date: Available online 8 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Morgan A. Hitchner, Luis E. Santiago-Ortiz, Matthew R. Necelis, David J. Shirley, Thaddeus J. Palmer, Katharine Tarnawsky, Timothy D. Vaden, Gregory A. CaputoAbstractAntimicrobial peptides (AMPs) have been an area of great interest, due to the high selectivity of these molecules toward bacterial targets over host cells and the limited development of bacterial resistance to these molecules throughout evolution. Previous work showed that when Histidine was incorporated into the peptide C18G it lost antimicrobial ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 8, 2019 Category: Biochemistry Source Type: research

Beyond electrostatics: Antimicrobial peptide selectivity and the influence of cholesterol-mediated fluidity and lipid chain length on protegrin-1 activity
Publication date: Available online 8 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): J. Michael Henderson, Nishanth S. Iyengar, Kin Lok H. Lam, Eddie Maldonado, Tiffany Suwatthee, Indroneil Roy, Alan J. Waring, Ka Yee C. LeeAbstractAntimicrobial peptides (AMPs) are a promising class of innate host defense molecules for next-generation antibiotics, as they uniquely target and permeabilize membranes of pathogens. This selectivity has been explained by the electrostatic attraction between these predominantly cationic peptides and the bacterial membrane, which is heavily populated with anionic lipi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 8, 2019 Category: Biochemistry Source Type: research

Molecular basis for the protective effects of low-density lipoprotein receptor-related protein 1 (LRP1)-derived peptides against LDL aggregation
Publication date: Available online 8 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Aleyda Benitez-Amaro, Chiara Pallara, Laura Nasarre, Andrea Rivas-Urbina, Sonia Benitez, Angela Vea, Olga Bornachea, David de Gonzalo-Calvo, Gabriel Serra-Mir, Sandra Villegas, Roger Prades, José Luís Sanchez-Quesada, Cristina Chiva, Eduard Sabido, Teresa Tarragó, Vicenta Llorente-CortésAbstractAggregated LDL is the first ligand reported to interact with the cluster II CR9 domain of low-density lipoprotein receptor-related protein 1 (LRP1). In particular, the C-terminal half of domai...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 8, 2019 Category: Biochemistry Source Type: research

Determination of δ-opioid receptor molecules mobility in living cells plasma membrane by novel method of FRAP analysis
Publication date: Available online 7 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Jiří Janáček, Jana Brejchová, Petr SvobodaAbstractFluorescence recovery after photobleaching (FRAP) is the preferred method for analyzing the lateral mobility of fluorescently-tagged proteins in the plasma membranes (PMs) of live cells. FRAP experiments are described as being easy to perform; however, the analysis of the acquired data can be difficult. The evaluation procedure must be properly combined with the imaging setup of the confocal microscope to provide unbiased results.With the ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 7, 2019 Category: Biochemistry Source Type: research

Ceramide phosphoethanolamine, an enigmatic cellular membrane sphingolipid
Publication date: Available online 5 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Anastasija Panevska, Matej Skočaj, Igor Križaj, Peter Maček, Kristina SepčićAbstractCeramide phosphoethanolamine (CPE) is the major sphingolipid in invertebrates and in some bacterial species. It has been also detected in mammalian cells, although only in trace amounts. Complete understanding of the biophysical and physiological relevance of CPE is still lacking, and its biological role is still an open question. CPE differs in its biosynthetic mechanisms from sphingomyelin, due to the specific CPE synthas...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 7, 2019 Category: Biochemistry Source Type: research

Hybrids made from antimicrobial peptides with different mechanisms of action show enhanced membrane permeabilization
Publication date: Available online 5 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Heidi M. Wade, Louise E.O. Darling, Donald E. ElmoreAbstractCombining two known antimicrobial peptides (AMPs) into a hybrid peptide is one promising avenue in the design of agents with increased antibacterial activity. However, very few previous studies have considered the effect of creating a hybrid from one AMP that permeabilizes membranes and another AMP that acts intracellularly after translocating across the membrane. Moreover, very few studies have systematically evaluated the order of parent peptides or ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 5, 2019 Category: Biochemistry Source Type: research

The SUMO-specific protease Senp2 regulates SUMOylation, expression and function of human organic anion transporter 3
We report here that overexpression of Senp2 in COS-7 cells led to a reduced OAT3 SUMOylation, which correlated well with a decreased OAT3 expression and transport activity. Such phenomenon was not observed in cells overexpressing an inactive mutant of Senp2. Furthermore, transfection of cells with Senp2-specific siRNA to knockdown the endogenous Senp2 resulted in an increased OAT3 SUMOylation, which correlated well with an enhanced OAT3 expression and transport activity. Coimmunoprecipitation experiments showed that Senp2 directly interacted with OAT3 in the kidneys of rats. Together these results provided first demonstrat...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - May 2, 2019 Category: Biochemistry Source Type: research

The effects of radioprotectant and potential antioxidant agent amifostine on the structure and dynamics of DPPC and DPPG liposomes
In this study, the effect of amifostine on neutral zwitterionic dipalmitoylphosphatidylcholine (DPPC) and anionic dipalmitoylphosphatidylglycerol (DPPG) model membranes' structure and dynamics is aimed to be examined by Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Our results revealed that amifostine at concentrations used (1–24 mol%) does not induce any important alteration in the shape of phase transition curve and phase transition temperature in the DPPC and DPPG membranes. High concentrations of amifostine slightly increased the acyl chain flexibility of DPPC membr...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 25, 2019 Category: Biochemistry Source Type: research

Embedding calix[4]resorcinarenes in liposomes: Experimental and computational investigation of the effect of resorcinarene inclusion on liposome properties and stability
Publication date: Available online 25 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Romina Zappacosta, Massimiliano Aschi, Alessandra Ammazzalorso, Pietro Di Profio, Antonella Fontana, Gabriella SianiAbstractTwo calix[4]resorcinarenes, which differ in the length of the alkyl chain on the methylene bridge between the aromatic rings, have been embedded in unilamellar liposomes prepared from 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine in three host/guest ratios, following two different procedures. The effect of the insertion of the guests has been evaluated through the measurements of th...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 25, 2019 Category: Biochemistry Source Type: research

Osmoprotective effect of ubiquinone in lipid vesicles modelling the E. coli plasma membrane
Publication date: Available online 23 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Emma K. Eriksson, Katarina Edwards, Philipp Grad, Lars Gedda, Víctor Agmo HernándezAbstractBacteria need to be able to adapt to sudden changes in their environment, including drastic changes in the surrounding osmolarity. As part of this adaptation, the cells adjust the composition of their cytoplasmic membrane. Recent studies have shown that ubiquinones, lipid soluble molecules involved in cell respiration, are overproduced by bacteria grown in hyperosmotic conditions and it is thus believed t...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 23, 2019 Category: Biochemistry Source Type: research

Biophysical characterization of mycobacterial model membranes and their interaction with rifabutin: Towards lipid-guided drug screening in tuberculosis
Publication date: Available online 17 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ruchika Dadhich, Abhishek Singh, Anjana P. Menon, Manjari Mishra, C.D. Athul, Shobhna KapoorAbstractLipid structure critically dictates the molecular interactions of drugs with membranes influencing passive diffusion, drug partitioning and accumulation, thereby underpinning a lipid-composition specific interplay. Spurring selective passive drug diffusion and uptake through membranes is an obvious solution to combat growing antibiotic resistance with minimized toxicities. However, the spectrum of complex myco...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 17, 2019 Category: Biochemistry Source Type: research

Ultrashort nanosecond electric pulses evoke heterogeneous patterns of Ca2+ release from the endoplasmic reticulum of adrenal chromaffin cells
Publication date: Available online 12 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Josette Zaklit, Indira Chatterjee, Normand Leblanc, Gale L. CravisoGraphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 13, 2019 Category: Biochemistry Source Type: research

New insight into phase behavior and permeability of skin lipid models based on sphingosine and phytosphingosine ceramides
In this study, we have investigated the influence of non-hydroxy and α-hydroxy sphingosine-based CERs and their phytosphingosine counterparts on the permeability and lipid organization of model membranes, which were adjusted in composition to enhance formation of the LPP. The permeability was compared by diffusion studies using ethyl-p-aminobenzoate as a model drug, and the lipid organization was characterized by X-ray diffraction and infrared spectroscopy. Both the sphingosine- and phytosphingosine-based CER models formed the LPP, while the latter exhibited a longer LPP repeat distance. The ethyl-p-aminobenzoate flu...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 13, 2019 Category: Biochemistry Source Type: research

Modulation of biological responses to 2 ns electrical stimuli by field reversal
Publication date: Available online 11 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Esin B. Sözer, P. Thomas VernierAbstractNanosecond bipolar pulse cancellation, a recently discovered phenomenon, is modulation of the effects of a unipolar electric pulse exposure by a second pulse of opposite polarity. This attenuation of biological response by reversal of the electric field direction has been reported with pulse durations from 60 ns to 900 ns for a wide range of endpoints, and it is not observed with conventional electroporation pulses of much longer duration (>100 μs) whe...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 13, 2019 Category: Biochemistry Source Type: research

A biophysical study of the interactions between the antimicrobial peptide indolicidin and lipid model systems
Publication date: Available online 9 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Josefine Eilsø Nielsen, Tania Kjellerup Lind, Abdullah Lone, Yuri Gerelli, Paul Robert Hansen, Håvard Jenssen, Marité Cárdenas, Reidar LundAbstractThe naturally occurring peptide indolicidin from bovine neutrophils exhibits strong biological activity against a broad spectrum of microorganisms. This is believed to arise from selective interactions with the negatively charged cytoplasmic lipid membrane found in bacteria. We have investigated the peptide interaction with supported lipi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 10, 2019 Category: Biochemistry Source Type: research

Sub-inhibitory membrane damage undermines Staphylococcus aureus virulence
Publication date: Available online 8 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ayelet Sarah Hershkovits, Igor Pozdnyakov, Ohad Meir, Amram MorAbstractWe investigated antibacterial properties of a recently described membrane-active lipopeptide, C10OOc12O (decanoyl-ornithyl-ornithyl-dodecanoyl-ornithyl-amide) against Gram-positive bacteria (GPB). Minimal inhibitory concentrations (MICs) and kinetics were compared in culture media and plasma. Chemo-sensitization to antibiotics was determined using the checkerboard assay. Membrane damages were estimated using diverse membrane potential sens...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 10, 2019 Category: Biochemistry Source Type: research

The intracellular NPxY motif is critical in maintaining the function and expression of human organic anion transporting polypeptide 1B1
Publication date: Available online 7 April 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Xuyang Wang, Yushuang Liang, Zihui Fang, Jiujiu Huang, Mei HongAbstractOrganic anion transporting polypeptides (OATPs, gene symbol SLCO) mediate sodium-independent transport of endogenous compounds such as bile salts, hormones and their conjugates as well as toxins and drugs. OATP1B1 is the major OATP specifically expressed at the basolateral membrane of human hepatocytes and many clinically important drugs have been shown to be substrates of the transporter. According to the computer-based hydropathy analysi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 7, 2019 Category: Biochemistry Source Type: research

Use of virus-like particles as a native membrane model to study the interaction of insulin with the insulin receptor
In this study, we used Chinese hamster ovary (CHO T10) cells stably overexpressing the insulin receptor (IR) to generate IR bearing VLPs. The diameter and size uniformity of VLPs were estimated by dynamic light scattering and morphological features examined by scanning electron microscopy. The presence of high affinity IR on VLPs was demonstrated by competitive binding assays (KD: 2.3 ± 0.4 nM, n = 3), which was similar to that on the parental CHO T10 cells (KD: 2.1 ± 0.4 nM, n = 3). We also report that increases or decreases in membrane cholesterol content by treatment with methyl-β-...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 3, 2019 Category: Biochemistry Source Type: research

Structural and mechanistic insights into EchAMP: A antimicrobial protein from the Echidna milk
ConclusionsRecombinant EchAMP exhibits broad-spectrum bacteriolytic activity by compromising the bacterial cell membrane integrity. Hence, we propose that this intrinsically disordered antimicrobial protein interact with the bacterial cell membrane and undergoes conformational changes to form channels in the membrane resulting in cell lysis.General significanceEchAMP, the evolutionarily conserved, lactation specific AMP from an oviparous mammal may find application as a broad-spectrum antimicrobial against pathogens that affect mammary gland or otherwise cause routine infections in humans and livestock.Graphical abstract (...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - April 3, 2019 Category: Biochemistry Source Type: research

Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria
In conclusion, PaDBS1R6 is a candidate for the treatment of nosocomial infections caused by Gram-negative bacteria as template for producing other antimicrobial agents.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - March 28, 2019 Category: Biochemistry Source Type: research