Single-channel permeability and glycerol affinity of human aquaglyceroporin AQP3
Publication date: Available online 17 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Roberto A. Rodriguez, Huiyun Liang, Liao Y. Chen, Germán Plascencia-Villa, George PerryAbstractFor its fundamental relevance, transport of water and glycerol across the erythrocyte membrane has long been investigated before and after the discovery of aquaporins (AQPs), the membrane proteins responsible for water and glycerol transport. AQP1 is abundantly expressed in the human erythrocyte for maintaining its hydrohomeostasis where AQP3 is also expressed (at a level ~30-folds lower than AQP1) facilit...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 17, 2019 Category: Biochemistry Source Type: research

Systematic mutational analysis of human neutrophil α-defensin HNP4
Publication date: Available online 15 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Han Hu, Bin Di, W. David Tolbert, Neelakshi Gohain, Weirong Yuan, Pan Gao, Bohan Ma, Qigai He, Marzena Pazgier, Le Zhao, Wuyuan LuAbstractDefensins are a family of cationic antimicrobial peptides of innate immunity with immunomodulatory properties. The prototypic human α-defensins, also known as human neutrophil peptides 1-3 or HNP1-3, are extensively studied for their structure, function and mechanisms of action, yet little is known about HNP4 – the much less abundant “distant cousin&rdq...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 16, 2019 Category: Biochemistry Source Type: research

Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin
In this study, we report that HBx specifically targets cardiolipin (CL) and induces membrane permeabilization depending on CL concentration in mitochondrial outer membrane–mimic artificial liposomes. Interestingly, HBx-induced membrane permeabilization was enhanced by liposomes containing phosphatidylethanolamine, which plays a crucial role in forming a negative curvature on the membrane. We also show that the 68-117 region of HBx, which interacts with mitochondria, is necessary for membrane permeabilization. We examined the size of the pores formed by HBx and found that HBx permeates fluorescent dyes depending on th...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 16, 2019 Category: Biochemistry Source Type: research

The role of membrane trafficking in the processing of amyloid precursor protein and production of amyloid peptides in Alzheimer's disease
Publication date: Available online 11 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Jing Zhi A. Tan, Paul A. GleesonAbstractAlzheimer's disease (AD) is characterized by progressive accumulation of misfolded proteins, which form senile plaques and neurofibrillary tangles, and the release of inflammatory mediators by innate immune responses. β-Amyloid peptide (Aβ) is derived from sequential processing of the amyloid precursor protein (APP) by membrane-bound proteases, namely the β-secretase, BACE1, and γ-secretase. Membrane trafficking plays a key role in the regulation...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 12, 2019 Category: Biochemistry Source Type: research

Domain redistribution within ergosterol-containing model membranes in the presence of the antimicrobial compound fengycin
Publication date: Available online 10 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Elisabeth Mantil, Trinda Crippin, Tyler J. AvisAbstractThe cyclic lipopeptide fengycin, produced by Bacillus subtilis, exhibits its antimicrobial capabilities by altering the integrity of the cell membrane of plant pathogens. Previous work has correlated fengycin activity with membrane characteristics, such as sterol content. This work focused on the influence of fengycin on supported lipid bilayers containing varying levels of ergosterol. Total internal reflection fluorescence (TIRF) microscopy was used t...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 11, 2019 Category: Biochemistry Source Type: research

Psd2 pea defensin shows a preference for mimetic membrane rafts enriched with glucosylceramide and ergosterol
Publication date: Available online 10 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Virginia Sara Grancieri do Amaral, Caroline Mota Fernandes, Mário R. Felício, Aline Sol Valle, Paula G. Quintana, Caroline Correa de Almeida, Eliana Barreto-Bergter, Sónia Gonçalves, Nuno C. Santos, Eleonora KurtenbachAbstractPsd2 is a pea defensin with 47 amino acid residues that inhibits the growth of fungal species by an uncharacterized mechanism. In this work, Psd2 interactions with model membranes mimicking the lipid compositions of different organisms were evaluated. Prote...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 11, 2019 Category: Biochemistry Source Type: research

NMR analysis of free and lipid nanodisc anchored CEACAM1 membrane proximal peptides with Ca2+/CaM
Publication date: Available online 10 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Haike Ghazarian, Weidong Hu, Allen Mao, Tung Nguyen, Nagarajan Vaidehi, Stephen Sligar, John E. ShivelyAbstractCEACAM1, a homotypic transmembrane receptor with 12 or 72 amino acid cytosolic domain isoforms, is converted from inactive cis-dimers to active trans-dimers by calcium-calmodulin (Ca2+/CaM). Previously, the weak binding of Ca2+/CaM to the human 12 AA cytosolic domain was studied using C-terminal anchored peptides. We now show the binding of 15N labeled Phe-454 cytosolic domain peptides in solution...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 11, 2019 Category: Biochemistry Source Type: research

New ionic targets of 3,3′,5′-triiodothyronine at the plasma membrane of rat Sertoli cells
Publication date: Available online 9 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ana Paula Zanatta, Renata Gonçalves, Leila Zanatta, Geisel Túlio de Oliveria, Ana Luiza Ludwig Moraes, Ariane Zamoner, Víctor Fernández-Dueñas, Débora Lanznaster, Francisco Ciruela, Carla Inês Tasca, Christelle Delalande, Danusa Menegaz, Fátima Regina Mena Barreto SilvaAbstractThe functions of Sertoli cells, which structurally and functionally support ongoing spermatogenesis, are effectively modulated by thyroid hormones, amongst other molecules. We inv...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 10, 2019 Category: Biochemistry Source Type: research

Membrane cholesterol depletion reduces downstream signaling activity of the adenosine A2a receptor
Publication date: Available online 8 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Claire McGraw, Lewen Yang, Ilya Levental, Edward Lyman, Anne Skaja-RobinsonAbstractCholesterol has been shown to modulate the activity of multiple G Protein-coupled receptors (GPCRs), yet whether cholesterol acts through specific interactions, indirectly via modifications to the membrane, or via both mechanisms is not well understood. High-resolution crystal structures of GPCRs have identified bound cholesterols; based on a β2-adrenergic receptor (β2AR) structure bound to cholesterol and the prese...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 8, 2019 Category: Biochemistry Source Type: research

Structural characterization of cationic DODAB bilayers containing C24:1 β-glucosylceramide
Publication date: Available online 3 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Letícia S. Martins, Daniela A. Nomura, Evandro L. Duarte, Karin A. Riske, M. Teresa Lamy, Julio H.K. RozenfeldAbstractThe effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 β-glucosylceramide (βGlcCer) on the structure of cationic DODAB bilayers was investigated by means of differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy and fluorescence microscopy. βGlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed in...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 4, 2019 Category: Biochemistry Source Type: research

Insights into the structure and molecular topography of the fatty acylated domain of synaptotagmin-1
Publication date: Available online 4 January 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Piotr Ruchala, Alan J. Waring, Marianne Cilluffo, Julian P. Whitelegge, Cameron B. GundersenAbstractAbundant attention has focused on synaptotagmin's C2 domains, but less is known about the structure and function of its other regions. Here, we synthesized the N-acetylated, C-end amidated and Cys-palmitated peptide (VLTCCFCICK KCLFKKKNKK K) which includes the fatty acylated cysteine residues in the membrane-affiliated domain of synaptotagmin-1. Fourier-transform infrared spectrometry indicated that this pept...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - January 4, 2019 Category: Biochemistry Source Type: research

Contrasting roles of oxidized lipids in modulating membrane microdomains
Publication date: Available online 31 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Tayana Mazin Tsubone, Helena Couto Junqueira, Maurício S. Baptista, Rosangela ItriAbstractLipid rafts display a lateral heterogeneity forming membrane microdomains that hold a fundamental role on biological membranes and are indispensable to physiological functions of cells. Oxidative stress in cellular environments may cause lipid oxidation, changing membrane composition and organization, thus implying in effects in cell signaling and even loss of homeostasis. The individual contribution of oxidiz...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 31, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: 1 February 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 2Author(s): (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 25, 2018 Category: Biochemistry Source Type: research

Rosmarinic acid and its esters inhibit membrane cholesterol domain formation through an antioxidant mechanism based, in nonlinear fashion, on alkyl chain length
ConclusionThese data indicate that the membrane antioxidant and cholesterol domain inhibition activities of rosmarinic acid esters are dependent, nonlinearly, on alkyl chain length. The mechanism for this effect is attributed to the influence of alkyl chain length on the optimal depth of the polyphenols into the lipid bilayer for trapping free radicals.General significanceThese findings provide insight into novel atheroprotective benefits of polyphenol esters that are dependent on their membrane location.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 22, 2018 Category: Biochemistry Source Type: research

Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
This study aims to investigate bacteriorhodopsin (bR) molecules reconstituted in lipid bilayers composed of di(nonafluorotetradecanoyl)-phosphatidylcholine (F4-DMPC), a partially fluorinated analogue of dimyristoyl-phosphatidylcholine (DMPC) to clarify the effects of partially fluorinated hydrophobic chains of lipids on protein's stability. Calorimetry measurements showed that the chain-melting transition of F4-DMPC/bR systems occurs at 3.5 °C, whereas visible circular dichroism (CD) and X-ray diffraction measurements showed that a two-dimensional (2D) hexagonal lattice formed by bR trimers in F4-DMPC bilayers remain...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 22, 2018 Category: Biochemistry Source Type: research

Effect of synthetic surfactants and soapwort (Saponaria officinalis L.) extract on skin-mimetic model lipid monolayers
Publication date: Available online 21 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ilona Jurek, Ilona Góral, Zofia Mierzyńska, Barbara Moniuszko-Szajwaj, Kamil WojciechowskiAbstractThe effect of a saponin-rich extract from rhizomes of Soapwort (Saponaria officinalis L) and four synthetic surfactants: sodium lauryl sulphate (SLS), sodium laureth sulphate (SLES), ammonium lauryl sulphate (ALS) and cocamidopropyl betaine (CAPB) on two model lipid monolayers is analyzed using surface pressure, surface dilatational rheology and fluorescence microscopy. The following monolayers were e...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 21, 2018 Category: Biochemistry Source Type: research

Interaction of statins with phospholipid bilayers studied by solid-state NMR spectroscopy
Publication date: Available online 20 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Leisan F. Galiullina, Holger A. Scheidt, Daniel Huster, Albert Aganov, Vladimir KlochkovAbstractStatins are drugs that specifically inhibit the enzyme HMG-CoA reductase and thereby reduce the concentration of low-density lipoprotein cholesterol, which represents a well-established risk factor for the development of atherosclerosis. The results of several clinical trials have shown that there are important intermolecular differences responsible for the broader pharmacologic actions of statins, even beyond ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 20, 2018 Category: Biochemistry Source Type: research

Membrane-active antimicrobial peptide identified in Rana arvalis by targeted DNA sequencing
Publication date: Available online 20 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Tomislav Rončević, Lucija Krce, Marco Gerdol, Sabrina Pacor, Monica Benincasa, Filomena Guida, Ivica Aviani, Vedrana Čikeš-Čulić, Alberto Pallavicini, Ana Maravić, Alessandro TossiAbstractAntimicrobial peptides (AMPs) are naturally produced, gene encoded molecules with a direct antimicrobial activity against pathogens, often also showing other immune-related properties. Anuran skin secretions are rich in bioactive peptides, including AMPs, and we have reported a novel targeted sequencing appr...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 20, 2018 Category: Biochemistry Source Type: research

The transmembrane domain of the SNARE protein VAMP2 is highly sensitive to its lipid environment
Publication date: Available online 20 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Zahia Fezoua-Boubegtiten, Benoit Hastoy, Pier Scotti, Alexandra Milochau, Katell Bathany, Bernard Desbat, Sabine Castano, Jochen Lang, Reiko OdaAbstractNeurotransmitter and hormone exocytosis depends on SNARE protein transmembrane domains and membrane lipids but their interplay is poorly understood. We investigated the interaction of the structure of VAMP2, a vesicular transmembrane SNARE protein, and membrane lipid composition by infrared spectroscopy using either the wild-type transmembrane domain (TMD)...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 20, 2018 Category: Biochemistry Source Type: research

Effects of H-bonds on sugar binding to chitoporin from Vibrio harveyi
ConclusionsHydrogen-bonds contribute to sugar permeation.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 19, 2018 Category: Biochemistry Source Type: research

Effect of general anesthetics on the properties of lipid membranes of various compositions
Publication date: Available online 18 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): György Hantal, Balázs Fábián, Marcello Sega, Balázs Jójárt, Pál JedlovszkyAbstractComputer simulations of four lipid membranes of different compositions, namely neat DPPC and PSM, and equimolar DPPC-cholesterol and PSM-cholesterol mixtures, are performed in the presence and absence of the general anesthetics diethylether and sevoflurane both at 1 and 600 bar. The results are analyzed in order to identify membrane properties that are potentially relat...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 18, 2018 Category: Biochemistry Source Type: research

Carborane derivatives of 1,2,3-triazole depolarize mitochondria by transferring protons through the lipid part of membranes
Publication date: Available online 15 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Tatyana I. Rokitskaya, Ljudmila S. Khailova, Anton V. Makarenkov, Alexei V. Shunaev, Victor V. Tatarskiy, Alexander A. Shtil, Valentina A. Ol'shevskaya, Yuri N. AntonenkoAbstractBoron containing polyhedra (carboranes) are three-dimensional delocalized aromatic systems. These structures have been shown to transport protons through lipid membranes and mitochondria. Conjugation of carboranes to various organic moieties is aimed at obtaining biologically active compounds with novel properties. Taking advantag...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 16, 2018 Category: Biochemistry Source Type: research

A novel amphipathic cell-penetrating peptide based on the N-terminal glycosaminoglycan binding region of human apolipoprotein E
Publication date: Available online 15 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Takashi Ohgita, Yuki Takechi-Haraya, Ryo Nadai, Mana Kotani, Yuki Tamura, Karin Nishikiori, Kazuchika Nishitsuji, Kenji Uchimura, Koki Hasegawa, Kumiko Sakai-Kato, Kenichi Akaji, Hiroyuki SaitoAbstractIn the direct cell membrane penetration, arginine-rich cell-penetrating peptides are thought to penetrate into cells across the hydrophobic lipid membranes. To investigate the effect of the amphipathic property of arginine-rich peptide on the cell-penetrating ability, we designed a novel amphipathic cell-pen...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 16, 2018 Category: Biochemistry Source Type: research

Receptor- and store-operated mechanisms of calcium entry during the nanosecond electric pulse-induced cellular response
Publication date: Available online 12 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Gleb P. Tolstykh, Jody C. Cantu, Melissa Tarango, Bennett L. IbeyAbstractNanosecond electric pulses have been shown to open nanopores in the cell plasma membrane by fluorescent imaging of calcium uptake and fluorescent dyes, including propidium (Pr) iodide and YO-PRO-1 (YP1). Recently, we demonstrated that nsEPs also induce the phosphoinositide intracellular signaling cascade by phosphatidylinositol‑4,5‑bisphosphate (PIP2) depletion resulting in physiological responses similar to those observed follow...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 13, 2018 Category: Biochemistry Source Type: research

The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes
Publication date: Available online 12 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): William T. Heller, Piotr A. ZolnierczukAbstractA short sequence on the gp41 envelope protein of HIV-1 is integral to infection by the virus. Without this sequence, termed the fusion peptide (FP), the virus is far less effective at fusing with the cellular membrane. One of the interesting features of the isolated FP is that it transitions between an α-helical conformation and a β-sheet conformation in lipid bilayer membranes as a function of lipid composition and concentration, and the transitio...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 12, 2018 Category: Biochemistry Source Type: research

Peptide antibiotic trichogin in model membranes: Self-association and capture of fatty acids
Publication date: Available online 12 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Ekaterina F. Afanasyeva, Victoria N. Syryamina, Marta De Zotti, Fernando Formaggio, Claudio Toniolo, Sergei A. DzubaAbstractThe antimicrobial action of peptides in bacterial membranes is commonly related to their mode of self-assembling which results in pore formation. To optimize peptide antibiotic use for therapeutic purposes, a study on the concentration dependence of self-assembling process is thus desirable. In this work, we investigate this dependence for peptaibol trichogin GA IV (Tric) in the 1-pa...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 12, 2018 Category: Biochemistry Source Type: research

In vitro lipid transfer assays of phosphatidylinositol transfer proteins provide insight into the in vivo mechanism of ligand transfer
Publication date: Available online 10 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Candace Panagabko, Matilda Baptist, Jeffrey AtkinsonAbstractFluorescence resonance energy transfer (FRET) assays and membrane binding determinations were performed using three phosphatidylinositol transfer proteins, including the yeast Sec14 and two mammalian proteins PITPα and PITPβ. These proteins were able to specifically bind the fluorescent phosphatidylcholine analogue NBD-PC ((2-(12-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine)) and to tra...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 10, 2018 Category: Biochemistry Source Type: research

A homologous series of apoptosis-inducing N‑acylserinols: Thermotropic phase behavior, interaction with cholesterol and characterization of cationic N‑myristoylserinol-cholesterol-CTAB niosomes
Publication date: Available online 6 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): D. Sivaramakrishna, Muvva D. Prasad, Musti J. SwamyAbstractN‑Acylserinols (NASOHs) exhibit anti-cancer activity by elevating ceramide levels, and/or by activating proapoptotic effectors. In the present work we investigated the thermotropic phase behavior and supramolecular organization of a homologous series of NASOHs (number of C-atoms in the acyl chain, n = 8–18), and the interaction of N-myristoylserinol (NMSOH) with cholesterol, and characterized cationic niosomes made up of NMSOH, choleste...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 7, 2018 Category: Biochemistry Source Type: research

Membrane interaction of off-pathway prion oligomers and lipid-induced on-pathway intermediates during prion conversion: A clue for neurotoxicity
Publication date: Available online 5 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Sophie Combet, Fabrice Cousin, Human Rezaei, Sylvie NoinvilleAbstractSoluble oligomers of prion proteins (PrP), produced during amyloid aggregation, have emerged as the primary neurotoxic species, instead of the fibrillar end-products, in transmissible spongiform encephalopathies. However, whether the membrane is among their direct targets, that mediate the downstream adverse effects, remains a question of debate. Recently, questions arise from the formation of membrane-active oligomeric species generated ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 7, 2018 Category: Biochemistry Source Type: research

Different roles for aspartates and glutamates for cation permeation in bacterial sodium channels
Publication date: Available online 6 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Carlo Guardiani, Olena A. Fedorenko, Igor A. Khovanov, Stephen K. RobertsAbstractA key driving force for ion channel selectivity is represented by the negative charge of the Selectivity Filter carried by aspartate (D) and glutamate (E) residues. However, the structural effects and specific properties of D and E residues have not been extensively studied. In order to investigate this issue we studied the mutants of NaChBac channel with all possible combinations of D and E in the charged rings in position 19...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 7, 2018 Category: Biochemistry Source Type: research

Lowering line tension with high cholesterol content induces a transition from macroscopic to nanoscopic phase domains in model biomembranes
Publication date: Available online 5 December 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Wen-Chyan Tsai, Gerald W. FeigensonAbstractChemically simplified lipid mixtures are used here as models of the cell plasma membrane exoplasmic leaflet. In such models, phase separation and morphology transitions controlled by line tension in the liquid-disordered (Ld) + liquid-ordered (Lo) coexistence regime have been described [1]. Here, we study two four-component lipid mixtures at different cholesterol fractions: brain sphingomyelin (BSM) or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/1,2-diol...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 5, 2018 Category: Biochemistry Source Type: research

Molecular optimization of autotransporter-based tyrosinase surface display
This study provides a basis for broadening of the range of proteins that can be surface displayed and the development of new autotransporter-based processes in industrial-scale whole-cell biocatalysis.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 4, 2018 Category: Biochemistry Source Type: research

Interaction of Hsp90AA1 with phospholipids stabilizes membranes under stress conditions
In this study, we showed Hsp90AA1 interacts with phospholipid membrane with high affinity. Using the depth-dependent fluorescence-quenching with brominated lipids, we found Hsp90AA1 penetrated 10.7 Å into the hydrocarbon core of the lipid bilayer. Circular dichroism spectra studies showed Hsp90AA1 lost part of its α-helical structures upon interaction with phospholipid membrane. By assessing binding properties of the three Hsp90AA1 domains, we found Hsp90AA1 interacted into the lipid bilayer mainly toward its C-terminus domain (CTD). Using scanning electron microscopy, we examined the protection on host cell ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - December 2, 2018 Category: Biochemistry Source Type: research

Interaction of Artepillin C with model membranes: Effects of pH and ionic strength
Publication date: Available online 20 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Wallance Moreira Pazin, Gilia Cristine Marques Ruiz, Osvaldo N. Oliveira, Carlos José Leopoldo ConstantinoAbstractArtepillin C is the major constituent of green propolis, one of the most consumed products in popular medicine owing to its therapeutic effects, including antitumor activity. Artepillin C differs from other cinnamic acid derivatives due to the presence of two prenylated groups in its structure, believed to enhance access to the cell membrane and resulting in pharmacological activity. Th...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 21, 2018 Category: Biochemistry Source Type: research

Translating chemometric analysis into physiological insights from in vivo confocal Raman spectroscopy of the human stratum corneum
Publication date: Available online 19 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Stefania Perticaroli, Dawn J. Yeomans, Fred C. Wireko, John T. Webber, Kathy M. Werchowski, R. Thomas Cambron, Paula J. RayAbstractThe superficial layer of the skin, the stratum corneum (SC), consists of corneocytes surrounded by lipid regions and acts as a protective barrier for the body against water loss, toxic agents and microorganisms. As most substances permeate the stratum corneum through the lipid regions, lipid organization is considered crucial for the skin barrier function. Here, we investigate...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 19, 2018 Category: Biochemistry Source Type: research

Editorial Board
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 18, 2018 Category: Biochemistry Source Type: research

Reviewer Acknowledgement
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1861, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Biomembranes)
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 18, 2018 Category: Biochemistry Source Type: research

The pore-forming action of polyenes: From model membranes to living organisms
Publication date: Available online 17 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Luka Kristanc, Bojan Božič, Špela Zemljič Jokhadar, Marija Sollner Dolenc, Gregor GomiščekAbstractThe incidence of resistant fungal pathogens has been increasing, especially in immuno-compromised people. As such, considerable research has been focused on discovering anti-fungal agents with new mechanisms of action and on optimizing the use of existing agents. In this context, interest in the polyene group of anti-fungals has recently been renewed, since they are known to be effective aga...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 17, 2018 Category: Biochemistry Source Type: research

BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy
Publication date: Available online 13 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Thomas Noe Perry, Hager Souabni, Chiara Rapisarda, Rémi Fronzes, Fabrice Giusti, Jean-Luc Popot, Manuela Zoonens, Francesca GubelliniAbstractMembrane protein (MP) complexes play key roles in all living cells. Their structural characterisation is hampered by difficulties in purifying and crystallising them. Recent progress in electron microscopy (EM) have revolutionised the field, not only by providing higher-resolution structures for previously characterised MPs but also by yielding first glimpses ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 14, 2018 Category: Biochemistry Source Type: research

Structural characterization of the L0 cytoplasmic loop of human multidrug resistance protein 6 (MRP6)
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Angela Ostuni, Maria Antonietta Castiglione Morelli, Flavia Cuviello, Alfonso Bavoso, Faustino BisacciaAbstractABCC6 is a member of the C subfamily of ATP-binding cassette transporters whose mutations are correlated to Pseudoxanthoma elasticum, an autosomal recessive, progressive disorder characterized by ectopic mineralization and fragmentation of elastic fibers. Structural studies of the entire protein have been hindered by its large size, membrane association, and domain complexity. Studies previously ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 12, 2018 Category: Biochemistry Source Type: research

Sequence and length optimization of membrane active coiled coils for triggered liposome release
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Camilla Skyttner, Robert Selegård, Jakob Larsson, Christopher Aronsson, Karin Enander, Daniel AiliAbstractDefined and tunable peptide-lipid membrane interactions that trigger the release of liposome encapsulated drugs may offer a route to improve the efficiency and specificity of liposome-based drug delivery systems, but this require means to tailor the performance of the membrane active peptides. In this paper, the membrane activity of a de novo designed coiled coil peptide has been optimized with ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 12, 2018 Category: Biochemistry Source Type: research

Enhanced expression of the Epithelial Sodium Channel in neutrophils from hypertensive patients
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Erika Reus-Chavarría, Ivette Martínez-Vieyra, Cristina Salinas-Nolasco, Araceli Evangelina Chávez-Piña, Juan Vicente Méndez-Méndez, Edgar Oliver López-Villegas, Alejandro Sosa-Peinado, Doris CerecedoAbstractHypertension (HTN), i.e. abnormally high blood pressure, is a major risk factor for heart attack, stroke, and kidney failure. The Epithelial Sodium Channel (ENaC), one of the main transporters regulates blood pressure by tightly controlling the sodium re...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 12, 2018 Category: Biochemistry Source Type: research

Roles of α-Methyl trans-Cyclopropane Groups in Behavior of Mixed Mycolic Acid Monolayers
Publication date: Available online 8 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Masumi Villeneuve, Hiroki NoguchiAbstractMixed Langmuir films of type 1 alpha- (α-) and keto-mycolic acids (MAs) were investigated to understand the roles of α-methyl trans-cyclopropane containing keto-MA in determining the physical and chemical properties of the monolayers. Surface pressure (π) vs. mean molecular area (A) isotherms were measured at constant mole fractions defined as the ratio of the keto-MA molarity to the total molarity of α-MA and keto-MA (Xketo) at 25 °C and ...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 10, 2018 Category: Biochemistry Source Type: research

E2P-like states of plasma membrane Ca2+‑ATPase characterization of vanadate and fluoride-stabilized phosphoenzyme analogues
Publication date: Available online 9 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Nicolás A. Saffioti, Marilina de Sautu, Mariela S. Ferreira-Gomes, Rolando C. Rossi, Joshua Berlin, Juan Pablo F.C. Rossi, Irene C. MangialavoriAbstractThe plasma membrane Ca2+‑ATPase (PMCA) belongs to the family of P-type ATPases, which share the formation of an acid-stable phosphorylated intermediate as part of their reaction cycle. The crystal structure of PMCA is currently lacking. Its abundance is approximately 0.1% of the total protein in the membrane, hampering efforts to produce suitable c...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 10, 2018 Category: Biochemistry Source Type: research

The lipid environment of Escherichia coli Aquaporin Z
In this study, we have investigated the lipids surrounding AqpZ, and the effects of a destabilizing mutation W14A (Schmidt and Sturgis 2017) on lipid protein interactions. In a first approach, we used Styrene Maleic Acid copolymer to prepare AqpZ containing nanodiscs, and these were analyzed for their lipid content, investigating both the lipid head-group and acyl-chain compositions. These results were complemented by native mass spectrometry of purified AqpZ in the presence of lipids, to give insights of variations in lipid binding at the surface of AqpZ. In an effort to gain molecular insights, to aid interpretation of t...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 8, 2018 Category: Biochemistry Source Type: research

A kinetic comparison between E2P and the E2P-like state induced by a beryllium fluoride complex in the Na,K-ATPase. Interactions with Rb+
Publication date: Available online 7 November 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Santiago Enrique Faraj, Mercedes Centeno, Rolando Carlos Rossi, Mónica Raquel MontesAbstractMetal-fluoride complexes have been used to induce E2P-like states with the aim of studying the events that occur during E2P hydrolysis in P-type ATPases. In the present work, we compared the E2P-like state induced by a beryllium fluoride complex (BeFx) with the actual E2P state formed through backdoor phosphorylation of the Na,K-ATPase. Formation of E2P and E2P-like states were investigated employing the styr...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 7, 2018 Category: Biochemistry Source Type: research

Cardiolipin mediates curcumin interactions with mitochondrial membranes
Publication date: Available online 31 October 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Shani Ben Zichri, Sofiya Kolusheva, Michael Danilenko, Saniya Ossikbayeva, William J. Stabbert, Juan L. Poggio, David E. Stein, Zulfiya Orynbayeva, Raz JelinekAbstractCurcumin, the main molecular ingredient of the turmeric spice, has been reported to exhibit therapeutic properties for varied diseases and pathological conditions. While curcumin appears to trigger multiple signaling pathways, the precise mechanisms accounting for its therapeutic activity have not been deciphered. Here we show that curcumin e...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 1, 2018 Category: Biochemistry Source Type: research

Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum
Publication date: Available online 31 October 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Yuzhu Han, Jianjun Zhao, Bao Zhang, Qian Shen, Qingmao Shang, Pinglan LiAbstractP852, a novel cyclic peptide isolated from Bacillus amyloliquefaciens L-H15, showed potent antifungal activity against several major plant fungal pathogens including Fusarium oxysporum. To elucidate the antifungal mechanism, the impact of P852 on the cell morphology and membrane permeabilization of F. oxysporum was studied. By applying electron microscopy and fluorescent techniques, we showed that P852 treatment caused the morp...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - November 1, 2018 Category: Biochemistry Source Type: research

A potent antibacterial activity of new short d-enantiomeric lipopeptide against multi drug resistant bacteria
Publication date: Available online 26 October 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Jaeho Lee, Shanghyeon Kim, Ji-Yeong Sim, Daeun Lee, Ha Hyung Kim, Jae Sam Hwang, Dong Gun Lee, Zee-Yong Park, Jae Il KimAbstractThe emergence of drug-resistant pathogenic bacteria threatens human health. Resistance to existing antibiotics is increasing, while the emergence of new antibiotics is slowing. Cationic antimicrobial peptides (CAMPs) are fascinating alternative antibiotics because they possess a broad spectrum of activity, being active against both Gram-positive and Gram-negative bacteria includin...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - October 27, 2018 Category: Biochemistry Source Type: research

Dynamic single-vesicle tracking of cell-bound membrane vesicles on resting, activated, and cytoskeleton-disrupted cells
Publication date: Available online 26 October 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Wendiao Zhang, Ye Xu, Guo Chen, Kun Wang, Wenzhe Shan, Yong ChenAbstractThe composition, structure, production, motion, fate, and functions of cell-bound membrane vesicles pre-existing in the plasma membrane of cells are poorly understood. Here, single-vesicle tracking of individual cell-bound membrane vesicles in the plasma membrane of endothelial cells treated with or without various reagents was performed to investigate the motion of cell-bound membrane vesicles. The efficacy of each of these reagents w...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - October 26, 2018 Category: Biochemistry Source Type: research