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Therapy: Gene Therapy

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Total 490 results found since Jan 2013.

Contactless delivery of plasmid encoding EGFP in vivo by high-intensity pulsed electromagnetic field
In conclusion, feasibility of HI-PEMF was demonstrated for transfection of different tissues (muscle, skin, tumor) and could have great potential in gene therapy and in DNA vaccination.PMID:34058542 | DOI:10.1016/j.bioelechem.2021.107847
Source: Bioelectrochemistry - May 31, 2021 Category: Biochemistry Authors: Matej Kranjc Simona Kranjc Brezar Gregor Ser ša Damijan Miklav čič Source Type: research

MRI-Visible Nanovehicle for Efficient siRNA Delivery
Methods Mol Biol. 2021;2282:195-208. doi: 10.1007/978-1-0716-1298-9_13.ABSTRACTVisualizing siRNA delivery through medical imaging methods has drawn much attentions in recent gene therapy studies. Among them, iron oxide-based magnetic resonance imaging (MRI) is regarded as one of the most promising imaging modalities for its high spatial resolution as well as deep penetration and real-time properties. In this chapter, a detailed protocol of an amphiphilic superparamagnetic iron oxide (SPIO) nanovehicle-based siRNA delivery is described, mainly focusing on SPIO/siRNA complexes formation and characterization, in vitro and in ...
Source: Mol Biol Cell - April 30, 2021 Category: Molecular Biology Authors: Rongrong Jin Wencheng Zhu Gan Lin Gang Liu Hua Ai Source Type: research

Gene therapy in the anterior eye segment
Curr Gene Ther. 2021 Apr 22. doi: 10.2174/1566523221666210423084233. Online ahead of print.ABSTRACTThis review provides comprehensive information about the advances in gene therapy in the anterior segment of the eye including cornea, conjunctiva, lacrimal gland, and trabecular meshwork. We discuss gene delivery systems including viral and non-viral vectors as well as gene editing techniques, mainly CRISPR-Cas9, and epigenetic treatments including antisense and siRNA therapeutics. We also provide a detailed analysis of various anterior segment diseases where gene therapy has been tested with corresponding outcomes. Disease ...
Source: Current Gene Therapy - April 27, 2021 Category: Genetics & Stem Cells Authors: Cynthia Amador Ruchi Shah Sean Ghiam Andrei A Kramerov Alexander V Ljubimov Source Type: research

Metformin-conjugated micellar system with intratumoral pH responsive de-shielding for co-delivery of doxorubicin and nucleic acid.
Abstract A novel PMet-P(cdmPEG2K) polymeric micellar carrier was developed for tumor-targeted co-delivery of DOX and nucleic acids (NA), based on polymetformin and a structure designed to lose the PEG shell in response to the acidic extracellular tumor environment. NA/DOX co-loaded micelleplexes exhibited enhanced inhibition of cell proliferation compared to DOX-loaded micelles, and displayed a higher level of cytotoxicity at an acidic pH (6.8) which mimicks the tumor microenvironment. The PMet-P(cdmPEG2K) micelles achieved significantly improved transfection with either a reporter plasmid or Cy3-siRNA, and enhanc...
Source: Biochemical Pharmacology - February 2, 2021 Category: Drugs & Pharmacology Authors: Liu Y, Sun J, Huang Y, Chen Y, Li J, Liang L, Xu J, Wan Z, Zhang B, Li Z, Li S Tags: Biochem Pharmacol Source Type: research

ZEB2 knock-down induces apoptosis in human myeloid leukemia HL-60 cells.
CONCLUSION: Our study results suggest that ZEB2 suppression in myeloid leukemia cells through apoptosis induction could be a proper therapeutic method. PMID: 33475058 [PubMed - as supplied by publisher]
Source: Current Gene Therapy - January 20, 2021 Category: Genetics & Stem Cells Authors: Fardi M, Mohammadi A, Baradaran B, Safaee S, Solali S Tags: Curr Gene Ther Source Type: research

Suppressing Sart1 to modulate macrophage polarization by siRNA-loaded liposomes: a promising therapeutic strategy for pulmonary fibrosis
Conclusion: Our study provides a valuable reference for modulating macrophage polarization and a promising strategy for the treatment of pulmonary fibrosis in clinical settings.
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Ting Pan, Qing Zhou, Kang Miao, Lei Zhang, Guorao Wu, Jun Yu, Yongjian Xu, Weining Xiong, Yong Li, Yi Wang Tags: Research Paper Source Type: research

Downregulation of histone-lysine N-methyltransferase EZH2 inhibits cell viability and enhances chemosensitivity in lung cancer cells.
Authors: Cao Z, Wu W, Wei H, Zhang W, Huang Y, Dong Z Abstract Histone-lysine N-methyltransferase EZH2 (EZH2) is the principle component of the polycomb repressive complex 2 (PRC2)/embryonic ectoderm development protein-EZH2 complex, which promotes tumorigenesis by repressing transcription of tumor suppressor genes. EZH2 is considered a key marker in several types of cancer, such as colorectal and prostate cancer. However, the molecular mechanisms and clinical value of EZH2 in lung cancer have not yet been fully investigated. The aim of the present study was to investigate the functions of EZH2 in lung cancer progr...
Source: Oncology Letters - November 27, 2020 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research