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Specialty: General Medicine
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Total 380 results found since Jan 2013.
Inhibition of prostate cancer DU145 cell growth with small interfering RNA targeting the SATB1 gene.
Authors: Wang Q, Yang CS, Ma ZX, Chen JC, Zheng JN, Sun XQ, Wang JQ
Abstract
Prostate cancer is a common visceral cancer of men worldwide. It is important to develop a more effective treatment for prostate cancer to overcome the treatment resistance that occurs with recurrence. RNA interference has been demonstrated to be a powerful tool for gene knockdown and has potential as a cancer treatment. It has been previously demonstrated that staining of special AT-rich sequence-binding protein 1 (SATB1) was stronger in prostatic carcinoma with metastasis compared with prostatic carcinoma without metastasis. In the prese...
Source: Experimental and Therapeutic Medicine - March 31, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
Roles of microRNA-539 and osteopontin in rheumatoid arthritis.
Authors: Liu Y, Qian K, Li C, Ma Y, Chen X
Abstract
The present study aimed to investigate the role and mechanism of microRNA-539 (miR-539) in rheumatoid arthritis (RA). A total of 68 RA patients and 46 osteoarthritis patients were enrolled into the current study. Peripheral blood and joint fluid were collected prior to treatment. Reverse transcription-quantitative polymerase chain reaction was performed to detect osteopontin (OPN) mRNA and miR-539 expression levels, while ELISA and western blot analysis were applied to detect OPN protein expression. In addition, bioinformatics analysis predicted that miR-539 direc...
Source: Experimental and Therapeutic Medicine - February 24, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
Association between DSCR4 gene methylation in plasma in early pregnancy and Down's syndrome.
Authors: Hu L, Zhou C
Abstract
Down's syndrome (DS), a chromosomal abnormal genetic disease caused by a local or total copy of chromosome 21, leads to patients suffering from delayed body growth, special facies, mild to moderate mental retardation and other symptoms, seriously affecting the life of patients. The aim of the present study was to examine the association between Down's syndrome critical region 4 (DSCR4) gene methylation in plasma in high-risk pregnant women with DS in early pregnancy (hereinafter referred to as pregnant women in early pregnancy) and DS, in order to screen new epigenetic markers for the...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor- κB signaling pathway.
In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-κB signaling pathway.
Exp Ther Med. 2018 Mar;15(3):2643-2648
Authors: Zhu H, Gao J, Wang L, Qian KJ, Cai LP
Abstract
The aim of the present study was to investigate the mechanism of action by which naringin reverses the resistance of ovarian cancer cells to cisplatin. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting assays were used to detect the effects of different concentrations of naringin on the expressions of nuclear factor (NF)-κB and P-gly...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3.
Authors: Meng X, Zhu Y, Tao L, Zhao S, Qiu S
Abstract
The present study aimed to investigate the association between Teashirt zinc finger homeobox 3 (TSHZ3) and the nucleus pulposus (NP) of intervertebral discs in rats. TSHZ3 was identified from the differentially expressed micro (mi)RNAs in the expression profile of GSE63492 by identifying the overlapped target genes of microRNA (miR)-125b-1-3p across different databases. TSHZ3 small interfering RNA (siRNA) and an miR-125b-1-3p inhibitor were used for gene silencing and gene silencing efficiency was assessed by reverse transcription-polymerase chain reaction. West...
Source: Experimental and Therapeutic Medicine - February 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
MicroRNA-185 suppresses pancreatic cell proliferation by targeting transcriptional coactivator with PDZ-binding motif in pancreatic cancer.
Authors: Xia D, Li X, Niu Q, Liu X, Xu W, Ma C, Gu H, Liu Z, Shi L, Tian X, Chen X, Zhang Y
Abstract
The aim of the present study was to compare the expression of transcriptional coactivator with the PDZ-binding motif (TAZ) in pancreatic cancer (PC) patients, and to investigate the regulation mechanisms of TAZ in the proliferation of PC. PC tissues and matched peritumoral tissues, pancreatic juice and serum were collected from PC patients who underwent pancreatectomy between June 2012 and December 2015 at the Affiliated Hospital of Qingdao University (Qingdao, China). Pancreatic juice and serum were collected from ...
Source: Experimental and Therapeutic Medicine - February 6, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
TRAP1 Regulates Autophagy in Lung Cancer Cells
Conclusions: Our results show that even though TRAP1 depletion affects both normal MRC‐5 and tumor A549 cell proliferation, inhibition of autophagy per se led to a decrease in tumor cell mass, while having a reduced effect on the normal cell line. The strategy of targeting TRAP1 in NSCLC shows future potential therapeutic applications.
This article is protected by copyright. All rights reserved.
Source: European Journal of Clinical Investigation - January 31, 2018 Category: General Medicine Authors: Ines A. Barbosa, Ignacio Vega ‐Naredo, Rute Loureiro, Ana F. Branco, Rita Garcia, Patricia M. Scott, Paulo J. Oliveira Tags: Original Article Source Type: research
Fibroblast growth factor 21 inhibition aggravates cardiac dysfunction in diabetic cardiomyopathy by improving lipid accumulation.
In conclusion, it is suggested that FGF21 may be a potentially useful agent in the treatment of DCM.
PMID: 29375676 [PubMed]
Source: Experimental and Therapeutic Medicine - January 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
Role of the Ca2+-Calcineurin-Nuclear Factor of Activated T cell Pathway in Mitofusin-2-Mediated Immune Function of Jurkat Cells.
CONCLUSIONS: Our findings suggest that MFN2 may regulate T cell immune functions primarily through the Ca2+-calcineurin-NFAT pathway. MFN2 may represent a potential therapeutic target for T cell immune dysfunction-related diseases.
PMID: 29363649 [PubMed - in process]
Source: Chinese Medical Journal - January 26, 2018 Category: General Medicine Authors: Xu XP, Yao YM, Zhao GJ, Wu ZS, Li JC, Jiang YL, Lu ZQ, Hong GL Tags: Chin Med J (Engl) Source Type: research
Anti-apoptosis Effect of Decoy Receptor 3 in Cholangiocarcinoma Cell Line TFK-1.
CONCLUSIONS: The effect of DcR3 on the growth and apoptosis of cholangiocarcinoma has been demonstrated. DcR3 is not only a predictive marker for malignant tumor but it is also likely to be a potential target for cancer gene therapy. Further studies should focus on exploring the binding ligand of DcR3, the signaling pathway involved, and the molecular mechanism for the regulation of DcR3 expression in cholangiocarcinoma.
PMID: 29271385 [PubMed - in process]
Source: Chinese Medical Journal - December 24, 2017 Category: General Medicine Authors: Xu YC, Cui J, Zhang LJ, Zhang DX, Xing BC, Huang XW, Wu JX, Liang CJ, Li GM Tags: Chin Med J (Engl) Source Type: research
Small interfering RNA against ERK1/2 attenuates cigarette smoke-induced pulmonary vascular remodeling.
This study investigated the potential role of ERK1/2 in the proliferation of rat PASMCs (rPASMCs) and cigarette smoke-induced PVR in rats. A small interfering RNA (siRNA) against ERK1/2 (ERK1/2-siRNA) was synthesized, and it significantly reduced the expression of ERK1/2 and cyclin E1, significantly increased the proportion of cells arrested at G0/G1 phase and significantly suppressed the proliferation of rPASMCs treated with cigarette smoke extract compared with controls (all P<0.05). In rats, ERK1/2-siRNA, which was administered intranasally, also inhibited the activation of ERK1/2 and the upregulation of cyclin E1, b...
Source: Experimental and Therapeutic Medicine - December 6, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research
Association between connexin 40 and potassium voltage-gated channel subfamily A member 5 expression in the atrial myocytes of patients with atrial fibrillation.
Authors: Zhang F, Bian Y, Huang L, Fan W
Abstract
Structural and electrical remodeling within the atrium mediate the pathogenesis of atrial fibrillation (AF). Two key genes that sever a role in this remodeling are connexin 40 (Cx40) and potassium voltage-gated channel subfamily A member 5 (KCNA5), respectively. Electrical remodeling is considered to induce structural remodeling during AF. In the present study, the left atrial appendage section and atrial myocytes of patients with AF were evaluated. It was observed that Cx40 and KCNA5 mRNA (P<0.05) and protein (P<0.01) expression was significantly downregulate...
Source: Experimental and Therapeutic Medicine - December 6, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research
MUC1 downregulation inhibits non-small cell lung cancer progression in human cell lines.
In conclusion, the findings indicated that silencing of MUC1 gene may inhibit the development of NSCLC cells.
PMID: 29104655 [PubMed]
Source: Experimental and Therapeutic Medicine - November 7, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research
Small interfering RNA directed against microRNA-155 delivered by a lentiviral vector attenuates asthmatic features in a mouse model of allergic asthma.
Authors: Chen H, Xu X, Cheng S, Xu Y, Xuefei Q, Cao Y, Xie J, Wang CY, Xu Y, Xiong W
Abstract
Asthma is a chronic T helper type 2 (Th2) cell-mediated inflammatory disease characterized by airway hyperresponsiveness (AHR) and airway inflammation. Although the majority of patients with asthma can achieve a good level of control with existing treatments, asthma runs a chronic course and the effectiveness of current treatment is not satisfactory for certain patients. MicroRNAs (miRNAs) are short noncoding RNAs that suppress gene expression at the post-transcriptional level; their role in regulating allergic inflammatio...
Source: Experimental and Therapeutic Medicine - November 7, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research
Novel Survivin-Targeted Small Interfering RNA Delivered by Nanoparticles.
CONCLUSIONS: Our findings revealed that survivin suppression by siRNA may contribute to tumor inhibition through both proliferation inhibition and apoptosis promotion effect, and the pharmacokinetic characteristics serve as a fundamental role for further studies on its applicability for cancer therapy.
PMID: 29173363 [PubMed - in process]
Source: The American Journal of the Medical Sciences - November 1, 2017 Category: General Medicine Authors: Feng C, Wang T, Zhang Y, Qu K, Tang S Tags: Am J Med Sci Source Type: research
