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Total 675 results found since Jan 2013.

Synthesis and characterization of amino acid-functionalized calcium phosphate nanoparticles for siRNA delivery.
Abstract Small interfering RNAs (siRNA) are short nucleic acid fragments of about 20-27 nucleotides, which can inhibit the expression of specific genes. siRNA based RNAi technology has emerged as a promising method for the treatment of a variety of diseases. However, a major limitation in the therapeutic use of siRNA is its rapid degradation in plasma and cellular cytoplasm, resulting in short half-life. In addition, as siRNA molecules cannot penetrate into the cell efficiently, it is required to use a carrier system for its delivery. In this work, chemically and morphologically different calcium phosphate (CaP) n...
Source: Colloids and Surfaces - June 27, 2017 Category: Biotechnology Authors: Bakan F, Kara G, Cokol Cakmak M, Cokol M, Denkbas EB Tags: Colloids Surf B Biointerfaces Source Type: research

Synthesis and characterization of amino acid-functionalized calcium phosphate nanoparticles for siRNA delivery
Publication date: 1 October 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 158 Author(s): Feray Bakan, Goknur Kara, Melike Cokol Cakmak, Murat Cokol, Emir Baki Denkbas Small interfering RNAs (siRNA) are short nucleic acid fragments of about 20–27 nucleotides, which can inhibit the expression of specific genes. siRNA based RNAi technology has emerged as a promising method for the treatment of a variety of diseases. However, a major limitation in the therapeutic use of siRNA is its rapid degradation in plasma and cellular cytoplasm, resulting in short half-life. In addition, as siRNA molecules cannot penetrate...
Source: Colloids and Surfaces B: Biointerfaces - July 8, 2017 Category: Biochemistry Source Type: research

Galactose Derivative-Modified Nanoparticles for Efficient siRNA Delivery to Hepatocellular Carcinoma.
In this study, we assembled lipid/calcium/phosphate nanoparticles (LCP NPs) conjugated with eight types of galactoside derivatives and demonstrated that phenyl β-d-galactoside-decorated LCP NPs (L4-LCP NPs) exhibited a superior siRNA delivery into HCC cells compared to normal hepatocytes. VEGF siRNAs delivered by L4-LCP NPs downregulated VEGF expression in HCC in vitro and in vivo and led to a potent antiangiogenic effect in the tumor microenvironment of a murine orthotopic HCC model. The efficient delivery of VEGF siRNA by L4-LCP NPs that resulted in significant tumor regression indicates that phenyl galactoside could be...
Source: Biomacromolecules - May 29, 2018 Category: Biochemistry Authors: Huang KW, Lai YT, Chern GJ, Huang SF, Tsai CL, Sung YC, Chiang CC, Hwang PB, Ho TL, Huang RL, Shiue TY, Chen Y, Wang SK Tags: Biomacromolecules Source Type: research

Knockdown siRNA Targeting the Mitochondrial Sodium-Calcium Exchanger-1 Inhibits the Protective Effects of Two Cannabinoids Against Acute Paclitaxel Toxicity
AbstractTreatment with cannabidiol (CBD) or KLS-13019 (novel CBD analog), has previously been shown to prevent paclitaxel-induced mechanical allodynia in a mouse model of chemotherapy-induced peripheral neuropathy (CIPN). The mechanism of action for CBD- and KLS-13019-mediated protection now has been explored with dissociated dorsal root ganglion (DRG) cultures using small interfering RNA (siRNA) to the mitochondrial Na+ Ca2+ exchanger-1 (mNCX-1). Treatment with this siRNA produced a 50 –55% decrease in the immunoreactive (IR) area for mNCX-1 in neuronal cell bodies and a 72–80% decrease in neuritic IR area as determin...
Source: Journal of Molecular Neuroscience - May 9, 2019 Category: Neuroscience Source Type: research

Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research

Magnetically responsive hybrid nanoparticles for in vitro siRNA delivery to breast cancer cells
Publication date: June 2019Source: Materials Science and Engineering: C, Volume 99Author(s): Milene Dalmina, Frederico Pittella, Jelver Alexander Sierra, Gabriela Regina Rosa Souza, Adny Henrique Silva, André Avelino Pasa, Tânia Beatriz Creczynski-PasaAbstractShort interfering RNA (siRNA) showed to be a viable alternative to a better prognosis in cancer therapy. Nevertheless, the successful application of this strategy still depends on the development of nanocarriers for the safe delivery of siRNA into the diseased tissue, which mostly occurs by passive accumulation. When an external magnetic field is applied, magnetic n...
Source: Materials Science and Engineering: C - February 23, 2019 Category: Materials Science Source Type: research

Calcium-doped mesoporous silica nanoparticles as a lysosomolytic nanocarrier for amine-free loading and cytosolic delivery of siRNA
Publication date: 25 January 2020Source: Journal of Industrial and Engineering Chemistry, Volume 81Author(s): Eunshil Choi, Dong-Kwon Lim, Sehoon KimAbstractFor efficacious gene therapeutics, cytosolic transport of the endocytosed siRNA is crucial, not to mention a non-toxic delivery carrier composition. In this paper, we report facile achievement of amine-free loading and lysosomolytic delivery of siRNA in an unconventional way by using calcium (Ca2+)-doped mesoporous silica nanoparticles (CMSNs) as a host material along with a pore-loaded endosomal disruptor, chloroquine (CQ). It is demonstrated that CMSNs are capable of...
Source: Journal of Industrial and Engineering Chemistry - November 16, 2019 Category: Chemistry Source Type: research

Biocompatible co-loading vehicles for delivering both nanoplatin cores and siRNA to treat hepatocellular carcinoma
Publication date: Available online 26 October 2019Source: International Journal of PharmaceuticsAuthor(s): Minsi Li, Pengxuan Zhao, Ting Fan, Yan Chen, Xiaojuan Zhang, Chuanchuan He, Tan Yang, Robert J. Lee, Muhammad Waseem Khan, Shahid Masood Raza, Xiang Ma, Yao Lu, Guangya XiangAbstractBmi-1 is a gene related to malignant transformation in hepatocellular carcinoma (HCC). The liver cancer cells developed the ability to tolerate CDDP treatment with the elevation of Bmi-1. Bmi-1 is also an oncogene promoting malignance of tumor and an anti-cancer target in many studies. Herein, a biocompatible nanocarrier was designed in th...
Source: International Journal of Pharmaceutics - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Calcium based siRNA coating: a novel approach for knockdown of HER2 gene in MCF-7 cells using gold nanoparticles.
Authors: Sami A, Naqvi SMS, Qayyum M, Raza Rao A, Sabitaliyevich UY, Ahmad MS Abstract Surface functionalization of nanoparticles (NPs) for therapeutic siRNA delivery into cancer cells has gained interest. The present study was designed for surface functionalization of gold nanoparticles (AuNPs) for efficient siRNA delivery and knockdown in cancer cells. In order to achieve this objective, AuNPs were coated with HER2-siRNA in the presence of 11-mercaptoundecanoic acid (11-MUA), calcium chloride (CaCl2) and polyethyleneimine (PEI) in alternate charge bearing successive layers. MCF-7 cells were cultured and transfect...
Source: Cellular and Molecular Biology - October 14, 2020 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Silica-coated calcium phosphate nanoparticles for gene silencing of NF- κB p65 by siRNA and their impact on cellular players of inflammation
Biomaterials. 2021 Jul 7;276:121013. doi: 10.1016/j.biomaterials.2021.121013. Online ahead of print.ABSTRACTThe transcription factor NF-κB and its signaling cascade both play key roles in all inflammatory processes. The most critical member of the NF-κB transcription factor family is p65. We investigated the role of cationic silica-coated calcium phosphate nanoparticles (spherical, diameter by SEM 50-60 nm; zeta potential about +26 mV; stabilized by polyethyleneimine) carrying encapsulated siRNA against NF-κB p65 and their influence on inflamed cells. The nanoparticles were taken up by cells of the blood compartment inv...
Source: Biomaterials - July 12, 2021 Category: Materials Science Authors: Nataniel Bia łas Elena K M üller Matthias Epple Ingrid Hilger Source Type: research

Local delivery of siRNA-loaded calcium phosphate nanoparticles abates pulmonary inflammation
Publication date: Available online 8 August 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Annika Frede, Bernhard Neuhaus, Torben Knuschke, Munisch Wadwa, Sebastian Kollenda, Robert Klopfleisch, Wiebke Hansen, Jan Buer, Dunja Bruder, Matthias Epple, Astrid M. Westendorf The local interference of cytokine signaling mediated by siRNA-loaded nanoparticles might be a promising new therapeutic approach to dampen inflammation during pulmonary diseases. For the local therapeutic treatment of pulmonary inflammation, we produced multi-shell nanoparticles consisting of a calcium phosphate core, coated wit...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 9, 2017 Category: Nanotechnology Source Type: research

Multifunctional hybrid nanoparticles as magnetic delivery systems for siRNA targeting the HER2 gene in breast cancer cells
In this study, we prepared a magnetic hybrid nanostructure composed of iron oxide nanoparticles coated with caffeic acid and stabilized by layers of calcium phosphate and PEG-polyanion block copolymer for incorporation of siRNA. Transmission electron microscopy images showed monodisperse, neutrally charged compact spheres sized <100 nm. Dynamic light scattering and nanoparticle tracking analysis revealed that the nanostructure had an average hydrodynamic diameter of 130 nm. Nanoparticle suspensions remained stable over 42 days of storage at 4 and 25 °C. Unloaded caffeic acid–magnetic calcium phosphate (Caf-MCaP)...
Source: Materials Science and Engineering: C - December 26, 2019 Category: Materials Science Source Type: research

Local delivery of siRNA-loaded calcium phosphate nanoparticles abates pulmonary inflammation
The local interference of cytokine signaling mediated by siRNA-loaded nanoparticles might be a promising new therapeutic approach to dampen inflammation during pulmonary diseases. For the local therapeutic treatment of pulmonary inflammation, we produced multi-shell nanoparticles consisting of a calcium phosphate core, coated with siRNAs directed against pro-inflammatory mediators, encapsulated into poly(lactic-co-glycolic acid), and coated with a final outer layer of polyethylenimine. Nasal instillation of nanoparticles loaded with a mixture of siRNAs directed against different cytokines to mice suffering from TH1 cell-me...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 8, 2017 Category: Nanotechnology Authors: Annika Frede, Bernhard Neuhaus, Torben Knuschke, Munisch Wadwa, Sebastian Kollenda, Robert Klopfleisch, Wiebke Hansen, Jan Buer, Dunja Bruder, Matthias Epple, Astrid M. Westendorf Source Type: research

Naringenin Produces Neuroprotection Against LPS-Induced Dopamine Neurotoxicity via the Inhibition of Microglial NLRP3 Inflammasome Activation
Conclusions: This study demonstrated that NAR targeted microglial NLRP3 inflammasome to protect DA neurons against LPS-induced neurotoxicity. These findings suggest NAR might hold a promising therapeutic potential for PD. Background Parkinson's disease (PD) is the second most prevalent central nervous system (CNS) degenerative disease. It is characterized by slow and progressive loss of dopamine (DA) neurons in the midbrain substantia nigra (SN) with the accumulation of α-synuclein in Lewy bodies and neuritis (1). Although the etiology of PD remains unclear, amounts of studies have suggested that ne...
Source: Frontiers in Immunology - April 30, 2019 Category: Allergy & Immunology Source Type: research