Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.

In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worms. A distinct motility reduction was also observed in treated worms compared to non-treated counterparts. This is the first report of the amenability of S. digitata to the siRNA induced RNAi. The presence of inter-domain linkers of muscle-specific twitchin kinase and calcium-dependent protein kinase isoform CDPK1 together with what our results revealed suggest that SDNP is most likely a protein involved in muscle movement and growth and development of the nematode. Hence SDNP has the characteristics of a potential drug target. PMID: 29448039 [PubMed - as supplied by publisher]
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research

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Conclusions/SignificanceAlum-adjuvantedOv-103 andOv-RAL-2 vaccines have the potential of reducing infection and thus morbidity associated with onchocerciasis also in humans. The development of cytophilic antibodies, that function in antibody-dependent cellular cytotoxicity, is essential for a successful prophylactic vaccine against this infection.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
by Ferdinand Ngale Njume, Stephen Mbigha Ghogomu, Robert Adamu Shey, Lea Olive Tchouate Gainkam, Philippe Poelvoorde, Perrine Humblet, Joseph Kamgno, Annie Robert, Leon Mutesa, Christophe Lelubre, Evelina Edelweiss, Arnaud Poterszman, Susi Anheuser, Luc Vanhamme, Jacob SouopguiOnchocerca volvulus is the nematode pathogen responsible for human onchocerciasis also known as “River blindness”, a neglected tropical disease that affects up to 18 million people worldwide. Helminths Excretory Secretory Products (ESPs) constitute a rich repertoire of molecules that can be exploited for host-parasite relationship, diagno...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
ConclusionsNational programs need to regularly monitor and evaluate the performance and progress of their interventions, while envisaging the complete elimination of onchocerciasis from their territory. Factors hindering the targeted goal of interruption of parasite transmission need to be identified and remedial actions should be taken. If possible and appropriate, ATSs need to be implemented to accelerate disease elimination by 2025.
Source: Infectious Diseases of Poverty - Category: Infectious Diseases Source Type: research
Publication date: Available online 15 June 2019Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Roger K. Prichard, Timothy G. GearyAbstractMacrocyclic lactone (ML) anthelmintics are the most important class of anthelmintics because of our high dependence on them for the control of nematode parasites and some ectoparasites in livestock, companion animals and in humans. However, resistance to MLs is of increasing concern. Resistance is commonplace throughout the world in nematode parasites of small ruminants and is of increasing concern in horses, cattle, dogs and other animals. It is suspe...
Source: International Journal for Parasitology: Drugs and Drug Resistance - Category: Parasitology Source Type: research
Anti-Wolbachia therapy for onchocerciasis &lymphatic filariasis: Current perspectives. Indian J Med Res. 2019 Jun;149(6):706-714 Authors: Wan Sulaiman WA, Kamtchum-Tatuene J, Mohamed MH, Ramachandran V, Ching SM, Sazlly Lim SM, Hashim HZ, Inche Mat LN, Hoo FK, Basri H Abstract Onchocerciasis and lymphatic filariasis (LF) are human filarial diseases belonging to the group of neglected tropical diseases, leading to permanent and long-term disability in infected individuals in the endemic countries such as Africa and India. Microfilaricidal drugs such as ivermectin and albendazole have been used as t...
Source: Indian J Med Res - Category: Research Authors: Tags: Indian J Med Res Source Type: research
The global burden of onchocerciasis is the heaviest in sub-Saharan Africa. Studies have shown the importance of the role of Community-Directed Distributors (CDDs) and nurses in onchocerciasis control, but litt...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Conclusion Ivermectin might add a chapter on its "wonder" drug properties - with activity against asexual stages of the malaria parasite Plasmodium falciparum. Observed activities might be difficult to reach with current regimens but will be more relevant in future high-dose regimens under investigation. Further studies should be done to confirm these results in vitro as well as in vivo. PMID: 31109978 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Yong Yang1,2†, Xue Bai1†, Chengyao Li1†, Mingwei Tong3, Peihao Zhang2, Wei Cai4, Xiaolei Liu1* and Mingyuan Liu1,5* 1Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, China 2Wu Xi Medical School, Jiangnan University, Wuxi, China 3School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, China 4Affiliated Hospital of Jiangnan University, The Fourth People's Hospital of Wuxi City, Wuxi, China 5Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Di...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Surveys conducted in 1991 –1992 in the Mbam Valley (Cameroon) revealed that onchocerciasis was highly endemic, with community microfilarial loads (CMFL) > 100 microfilariae/snip in some villages. Also in 1991–1...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Abstract Despite the efforts employed for the control of onchocerciasis, the latter has remained a significant public health problem, due mainly to the lack of safe and effective adult worm drugs and/or microfilaricides that do not kill Loa loa microfilariae (mf). Serious adverse events have been encountered after administering ivermectin to some onchocerciasis patients coinfected with Loa loa. There is therefore, an urgent need for a macro and/or microfilaricidal drug which kills Onchocerca but not L. loa microfilariae. A total of 12 crude extracts from Milletia comosa and Annona senegalensis were prepared and sc...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
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