Harnessing the versatility of PLGA nanoparticles for targeted Cre-mediated recombination
Publication date: Available online 23 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ngoc B. Nguyen, Cheng-Han Chen, Yulong Zhang, Peng Zhao, Benjamin Wu, Reza ArdehaliAbstractLigand-dependent Cre recombinases are pivotal tools for the generation of inducible somatic mutants. This method enables spatial and temporal control of gene activity through tamoxifen administration, providing new avenues for studying gene function and establishing animal models of human diseases. While this paved the way for developmental studies previously deemed impractical, the generation of tissue-specific transg...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 24, 2019 Category: Nanotechnology Source Type: research

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer
Publication date: Available online 22 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Adel Al Fatease, Vidhi Shah, Duc X. Nguyen, Brianna Cote, Nicole LeBlanc, Deepa A. Rao, Adam WG AlaniAbstractThis work looks to improve the efficacy of Adriamycin (ADR) while mitigating its cardiotoxicity using combinations of micellar resveratrol (R): quercetin (Q) (mRQ) or R: curcumin (C) (mRC) in healthy mice and ovarian cancer xenograft models. Ovarian cancer cells, ES2-Luc, or A2780ADR are inoculated in mice (n = 4/group) and sorted into eight cohorts. Mice are treated weekly for 4 weeks ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 24, 2019 Category: Nanotechnology Source Type: research

Tumor-targeted drug delivery and sensitization by MMP2-responsive polymeric micelles
In this study, the micelles assembled by a matrix metalloproteinase 2 (MMP2)-sensitive self-assembling efflux inhibitor (PEG2k-pp-PE) were developed and evaluated in various cancer models. In vitro, the PEG2k-pp-PE micelles enhanced the cellular uptake and tissue penetration and sensitized the cancers to drug treatments in MDR cancer cells and their three-dimensional multicellular spheroids. Their efflux inhibitory capability was comparable to those of the well-known small-molecule P-glycoprotein (P-gp) inhibitor and polymeric P-gp inhibitor. In vivo, the PEG2k-pp-PE micelles could specifically and effectively deliver the ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 18, 2019 Category: Nanotechnology Source Type: research

Nanoemulsion strategy of pioglitazone for the treatment of skin inflammatory diseases
Publication date: Available online 18 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Lupe C. Espinoza, Marcelle Silva-Abreu, Ana C. Calpena, María J. Rodríguez-Lagunas, María-José Fábrega, María L. Garduño-Ramírez, Beatriz ClaresAbstractPioglitazone (PGZ) is a peroxisome proliferator-activated receptor agonist. Its role in the inflammatory response modulation opens the door for additional therapeutic applications. The purpose of this study was to develop a pioglitazone nanoemulsion (PGZ-NE) to investigate its anti-inflammatory efficacy ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 18, 2019 Category: Nanotechnology Source Type: research

3D printed scaffolds with hierarchical biomimetic structure for osteochondral regeneration
This study demonstrated that 3D printed scaffold with biphasic and biomimetic structure are promising for bone and cartilage regeneration. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 18, 2019 Category: Nanotechnology Source Type: research

Multilayer nanoscale functionalisation to treat disorders and enhance regeneration of bone tissue
Publication date: Available online 17 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ana Marina Ferreira, Chiara Tonda-Turo, Elena Mancuso, Piergiorgio GentileAbstractThe coatings application onto medical devices has experienced a continuous growth in the last few years. Medical device coating market is expected to grow at a CAGR of 5.16% to reach USD 10 million by 2023 due to the increasing geriatric population and the growing demand for continuous innovation. Layer-by-Layer (LbL) assembly represents a versatile method to modify the surface properties, in order to control cell interaction a...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 17, 2019 Category: Nanotechnology Source Type: research

Bronchial epithelial cell extracellular vesicles ameliorate epithelial–mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization
Publication date: Available online 11 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Shengyang He, Duanni Chen, Mengyun Hu, Li Zhang, Caihong Liu, Daniela Traini, Georges E. Grau, Zhengpeng Zeng, Junjuan Lu, Guanzhi Zhou, Lihua Xie, Shenghua SunAbstractChronic obstructive pulmonary disease (COPD) is partly characterized as epithelial–mesenchymal transition (EMT)-related airflow limitation. Extracellular vesicles (EVs) play crucial roles in the crosstalk between cells, affecting many diseases including COPD. Up to now, the roles of EVs in COPD are still debated. As we found in this inve...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 15, 2019 Category: Nanotechnology Source Type: research

C60 FULLERENES SELECTIVELY INHIBIT BKCa BUT NOT Kv CHANNELS IN PULMONARY ARTERY SMOOTH MUSCLE CELLS
Publication date: Available online 11 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mariia I. Melnyk, Irina V. Ivanova, Dariia O. Dryn, Yuriy I. Prylutskyy, Vasyl V. Hurmach, Maxim Platonov, Lina T. Al Kury, Uwe Ritter, Anatoly I. Soloviev, Alexander V. ZholosAbstractPossessing unique physical and chemical properties, C60 fullerenes are arising as a potential nanotechnological tool that can strongly affect various biological processes. Recent molecular modeling studies have shown that C60 fullerenes can interact with ion channels, but there is lack of data about possible effects of C60 mole...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 12, 2019 Category: Nanotechnology Source Type: research

Thrombolysis induced by intravenous Administration of Plasminogen Activator in Magnetoliposomes: Dual targeting by magnetic and thermal manipulation
Publication date: Available online 11 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Chih-Hsin Liu, Hao-Lung Hsu, Jyh-Ping Chen, Tony Wu, Yunn-Hwa MaAbstractIn previously published studies, intra-arterial (i.a.), but not intravenous (i.v.) delivery of recombinant tissue-type plasminogen activator (rtPA) immobilized on the surface of magnetic nanoparticles induces thrombolysis by magnetic targeting. We asked whether i.v. delivery of protected rtPA in a thermosensitive magnetoliposome (TML@rtPA) may achieve target thrombolysis. PEGylated TML@rtPA was optimized and characterized; controlled rel...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 12, 2019 Category: Nanotechnology Source Type: research

Bronchial epithelial cell extracellular vesicles ameliorate epithelial-mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization
Publication date: Available online 11 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Shengyang He, Duanni Chen, Mengyun Hu, Li Zhang, Caihong Liu, Daniela Traini, Georges E. Grau, Zhengpeng Zeng, Junjuan Lu, Guanzhi Zhou, Lihua Xie, Shenghua SunAbstractChronic obstructive pulmonary disease (COPD) is partly characterized as epithelial-mesenchymal transition(EMT)-related airflow limitation. Extracellular vesicles(EVs) play crucial roles in the crosstalk between cells, affecting many diseases including COPD. Up to now, the roles of EVs in COPD are still debated. As we found in this investigatio...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 12, 2019 Category: Nanotechnology Source Type: research

Rhamnolipid nanoparticles for in vivo drug delivery and photodynamic therapy
Publication date: Available online 12 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Gawon Yi, Jihwan Son, Jihye Yoo, Changhee Park, Heebeom KooAbstractHerein, we report the development of self-assembled nanoparticles using rhamnolipid, a biosurfactant. Rhamnolipid is produced by Pseudomonas aeruginosa, and has an amphiphilic structure that is suitable for the formation of a nanoparticle shell. These rhamnolipid nanoparticles were loaded with pheophorbide a (Pba), a hydrophobic photosensitizer. The resulting nanoparticles had about 136.1-nm-diameter spherical shapes and had excellent water s...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 12, 2019 Category: Nanotechnology Source Type: research

Surface protein engineering increases the circulation time of a cell membrane-based nanotherapeutic
Publication date: June 2019Source: Nanomedicine: Nanotechnology, Biology and Medicine, Volume 18Author(s): Sangeetha Krishnamurthy, Padmalosini Muthukumaran, Muthu Kumara Gnanasammandhan Jayakumar, Domenik Lisse, Nihar D. Masurkar, Chenjie Xu, Juliana M. Chan, Chester L. DrumAbstractMammalian cell membranes are often incompatible with chemical modifications typically used to increase circulation half-life. Using cellular nanoghosts as a model, we show that proline-alanine-serine (PAS) peptide sequences expressed on the membrane surface can extend the circulation time of a cell membrane derived nanotherapeutic. Membrane exp...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 7, 2019 Category: Nanotechnology Source Type: research

The phenotype of target pancreatic cancer cells influences cell death by magnetic hyperthermia with nanoparticles carrying gemicitabine and the pseudo-peptide NucAnt
Publication date: Available online 30 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mourad Sanhaji, Julia Göring, Pierre Couleaud, Antonio Aires, Aitziber L. Cortajarena, José Courty, Adriele Prina-Mello, Marcus Stapf, Robert Ludwig, Yuri Volkov, Alfonso Latorre, Álvaro Somoza, Rodolfo Miranda, Ingrid HilgerAbstractIn this paper we show that conjugation of magnetic nanoparticles (MNPs) with Gemcitabine and/or NucAnt (N6L) fostered their internalization into pancreatic tumor cells and that the coupling procedure did not alter the cytotoxic potential of the drugs. By treati...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 31, 2019 Category: Nanotechnology Source Type: research

CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic
Publication date: Available online 30 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Guillermo Aquino-JarquinAbstractRecently Jennifer Doudna's group discovered the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated protein 14 (Cas14), identified almost exclusively in a superphylum of extremophile archaea. The newly discovered Cas14 possesses a single-stranded (ss)DNA targeting activity —despite being two times smaller than Cas9—, a capability that might confer a defense against viruses with ssDNA genomes. Furthermore, by combining the non-specif...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 30, 2019 Category: Nanotechnology Source Type: research

Liposomes produced by microfluidics and extrusion: A comparison for scale-up purposes
Publication date: Available online 13 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Vidhi M. Shah, Duc X. Nguyen, Pragnesh Patel, Brianna Cote, Adel Al-Fatease, Yvonne Pham, Man Gia Huynh, Yeonhee Woo, Adam WG AlaniAbstractSuccessful liposomal formulations in the clinic are severely limited due to poor translational capability of the traditional bench techniques to clinical production settings. The gold standard for liposome bench manufacturing is a multi-step and parameter dependent extrusion method. Moreover, these parameters need re-optimization for clinical production. The microfluidics...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 29, 2019 Category: Nanotechnology Source Type: research

Sirtuin 6 mediated stem cell Cardiomyogenesis on protein coated Nanofibrous scaffolds
This study, thus, demonstrates that protein coated scaffolds can augment cardiomyogenic differentiation of stem cells through a combination of topographical and biomolecular signals.Graphical AbstractCollagen coated polymer nanofibers promote stem cell cardiomyogenesis through Sirtuin6-mediated Wnt signaling pathway. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 27, 2019 Category: Nanotechnology Source Type: research

Surface engineering of LENS-Ti-6Al-4V to obtain nano- and micro-surface topography for orthopedic application
Publication date: Available online 4 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): A Revathi, Mitun Das, Vamsi K Balla, D Devika, Dwaipayan Sen, Geetha ManivasagamAbstractTwo distinct surface topographies consisting of micro- and nano-surface were developed using laser texturing (LT) and anodization process respectively and their effect on the surface-related properties of Ti-6Al-4V fabricated using Laser Engineered Net Shaping (LENS) were determined. The topographies developed using laser texturing (25, 50 and 75% overlap) were examined using 3D profilometer, whereas, Field Emission Scanni...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 23, 2019 Category: Nanotechnology Source Type: research

Inactivation of common hospital acquired pathogens on surfaces and in air utilizing engineered water nanostructures (EWNS) based nano-sanitizers
In this study, aqueous suspensions of various active ingredients (A.I.s) were utilized to generate Engineered Water Nanostructures (EWNS) nano-sanitizers, containing the A.I. utilized to produced them (termed iEWNS). These iEWNS had nanoscale size, were loaded with ROS and contained the A.I. utilized to produce them. They were challenged with Influenza H1N1/PR/8 and Acinetobacter baumannii on surfaces and in air. The results indicate that a nanogram dose of A.I. is effective in producing significant inactivation in Influenza H1N1/PR/8 and Acinetobacter baumannii. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 21, 2019 Category: Nanotechnology Source Type: research

Pharmaceutical feasibility and flow characteristics of polymeric non-spherical particles
Publication date: Available online 21 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Omkar Mhatre, Srushti SodhaAbstractLast decade has seen emergence of particle shape as a critical design parameter to overcome several long standing problems associated with particulate drug delivery- non-specific drug effects, RES uptake, poor bioavailability, achieving controlled release profiles, predictable degradation profiles, longer circulation time and zero order release kinetics to name a few. Non-spherical particles have been synthesized by techniques ranging from classical solvent evaporation to s...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 21, 2019 Category: Nanotechnology Source Type: research

Nano-based approach to combat emerging viral (NIPAH virus) infection
Publication date: Available online 21 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Rout George Kerry, Santosh Malik, Yisehak Tsegaye Redda, Sabuj Sahoo, Jayanta Kumar Patra, Sanatan MajhiAbstractEmergence of new virus and their heterogeneity is growing at an alarming rate. Sudden outburst of Nipah virus (NiV) have raised serious question about their instant management using conventional medication and diagnostic measures. A coherent strategy with versatility and comprehensive perspective to confront the rising distress could perhaps be effectuated by implementation of nanotechnology. But i...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 21, 2019 Category: Nanotechnology Source Type: research

Nanobiotechnology as an emerging approach to combat malaria: A systematic review
This article is the first systematic review of the effects of biosynthesized nanoparticles on both malaria parasites (Plasmodium spp.) and relevant vectors.Graphical Abstract (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 21, 2019 Category: Nanotechnology Source Type: research

Cationic lipoplexes for treatment of cancer stem cell-derived murine lung tumors
This study outlines the fabrication of a lipoplex of SOX2 small interfering RNA (CL-siSOX2) for targeted treatment of SOX2-enriched, CSC-derived orthotopic and xenograft lung tumors in CB-17 SCID mice. CL-siSOX2 induced tumor contraction in cisplatin-naïve and cisplatin-treated groups by 85% and 94% respectively. Reduction in tumor weight and volume following treatment with CL-siSOX2 was associated with reduced protein expression of SOX2 and markers of tumor initiation, inflammation, invasion and metastasis in mice tumor xenografts. In addition, histological staining of lung tumor sections showed reduction in SOX2 exp...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 20, 2019 Category: Nanotechnology Source Type: research

Exploration of TiO2 nanoparticle mediated microdynamic therapy on Cancer treatment
Publication date: Available online 14 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiao Chu, Liang Mao, Omar Johnson, Kang Li, Jonathan Phan, Qingshui Yin, Lihua Li, Junying Zhang, Wei Chen, Yu ZhangAbstractRadical therapy takes advantage of the reactive oxygen species produced in greater quantities within tumor cells than in normal cells. Here, for the first time, we explore a TiO2 nanoparticle mediated microwave induced radical therapy (termed as Microdynamic Therapy) as a new cancer treatment method. The experiments in vitro and in vivo demonstrate that colloidal TiO2 nanoparticles coul...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 15, 2019 Category: Nanotechnology Source Type: research

Liposomes produced by microfluidics and extrusion- a comparison for scale-up purposes
Publication date: Available online 13 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Vidhi M. Shah, Duc X. Nguyen, Pragnesh Patel, Brianna Cote, Adel Al-Fatease, Yvonne Pham, Man Gia Huynh, Yeonhee Woo, Adam WG. AlaniGraphical abstractGraphical abstract shows two methods to prepare liposomes: extrusion, which has challenges with scale-up, and microfluidics, which is scale-up efficient. Both methods produce liposomes that are identical in size and in vitro and in vivo behavior. Thus, substitution of extrusion, the gold standard of making liposomes, with a precise and scalable microfluidics pl...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 14, 2019 Category: Nanotechnology Source Type: research

NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety
Publication date: Available online 13 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Sameh Obeid, Pei-Shan Sung, Benoit Le Roy, Ming-Li Chou, Shie-Lang Shieh, Celine Elie-Caille, Thierry Burnouf, Wilfrid BoireauAbstractPlasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs fo...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 14, 2019 Category: Nanotechnology Source Type: research

Pea-like Nanocabins enable autonomous cruise and step-by-step drug pushing for deep tumor inhibition
Publication date: Available online 8 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ming Wu, Jiaojiao Chen, Hanitrarimalala Veroniaina, Subhankar Mukhopadhyay, Ziheng Wu, Zhenghong Wu, Xiaole QiAbstractPea-like nanocabins (HA@APT§DOX) were designed for deeply tumor inhibition. The AS1411 aptamer (APT) constituted “core shelf” which guaranteed DOX “beans” could be embedded, while the outer HA acted as “pea shell” coating. During the circulation (primary orbit), HA@APT§DOX could autonomously cruise until leak through tumor vasculature. Upon tumor s...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 9, 2019 Category: Nanotechnology Source Type: research

Phase I/II trial of Nano-camptothecin CRLX101 with Capecitabine and radiotherapy as neoadjuvant treatment for locally advanced rectal Cancer
Publication date: Available online 8 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Hanna K. Sanoff, Dominic H. Moon, Dominic T. Moore, Jeremiah Boles, Courtney Bui, William Blackstock, Bert H. O'Neil, Somasundaram Subramaniam, Autumn J. McRee, Cheryl Carlson, Michael S. Lee, Joel E. Tepper, Andrew Z. WangAbstractCRLX101 is a nanoparticle-drug conjugate with a camptothecin payload. We assessed the toxicity and pathologic complete response (pCR) rate of CRLX101 with standard neoadjuvant chemoradiotherapy (CRT) in locally advanced rectal cancer. A single-arm study was conducted with a 3 +...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 9, 2019 Category: Nanotechnology Source Type: research

Magnetic 3D scaffold: A Theranostic tool for tissue regeneration and non-invasive imaging In vivo
We report an osteoconducting magnetic 3D scaffold using Fe2+ doped nano-hydroxyapatite-Alginate-Gelatin (AGHFe1) for Magnetic Resonance Imaging based non-invasive monitoring of bone tissue regeneration. In rat cranial defect model, the scaffold facilitated non-invasive monitoring of cell migration, inflammatory response and matrix deposition by unique changes in transverse relaxation time (T2). Cell infiltration resulted in a considerable increase in T2 from ~37 to ~62 msec, which gradually returned to that of native bone (~23 msec) by 90 days. We used this method to compare in vivo performance of scaffold w...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 9, 2019 Category: Nanotechnology Source Type: research

Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation
Publication date: Available online 8 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Juhee Shin, Yuhua Yin, Do Kyung Kim, Sun Yeul Lee, Wonhyung Lee, Joon Won Kang, Dong Woon Kim, Jinpyo HongAbstractMicroglia play a critical role in neuropathic pain. Since upregulated Foxp3 in microglia enhances tissue repair by resolving neuroinflammation in excitotoxin-induced neuronal death, it may attenuate neuropathic pain in a similar manner. Therefore, this study tests whether Foxp3 introduced with poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles (Foxp3 NPs) can alleviate neuropathic pain by in...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 9, 2019 Category: Nanotechnology Source Type: research

Surface protein engineering increases circulation time of cell membrane-based Nanotherapeutic
Publication date: Available online 8 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Sangeetha Krishnamurthy, Padmalosini Muthukumaran, Muthu Kumara Gnanasammandhan Jayakumar, Domenik Lisse, Nihar D. Masurkar, Chenjie Xu, Juliana M. Chan, Chester L. DrumAbstractMammalian cell membranes are often incompatible with chemical modifications typically used to increase circulation half-life. Using cellular nanoghosts as a model, we show that proline - alanine - serine (PAS) peptide sequences expressed on the membrane surface can extend the circulation time of a cell membrane derived nanotherapeutic....
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 8, 2019 Category: Nanotechnology Source Type: research

Yttrium oxide nanoparticles reduce the severity of acute pancreatitis caused by cerulein hyperstimulation
Publication date: Available online 6 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Amit Khurana, Pratibha Anchi, Prince Allawadhi, Vinay Kumar, Nilofer Sayed, Gopinath Packirisamy, Chandraiah GoduguAbstractOxidative stress plays a major role in acute pancreatitis (AP), leading to massive macrophage infiltration. Nanoyttria (NY), possesses potent free radical scavenging activity. As reactive oxygen species and inflammation play major role in AP, we hypothesized that NY may alleviate cerulein induced AP. NY ameliorated LPS induced oxidative stress in vitro. It reduced ROS, superoxide radical ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 8, 2019 Category: Nanotechnology Source Type: research

Safety and effectiveness of sodium colistimethate-loaded nanostructured lipid carriers (SCM-NLC) against P. aeruginosa: in vitro and in vivo studies following pulmonary and intramuscular administration
We describe the preparation and the in vivo efficacy and safety of sodium colistimethate-loaded nanostructured lipid carriers (SCM-NLC) by the pulmonary and intramuscular routes. Nanoparticles showed 1-2 mg/L minimum inhibitory concentration against eight extensively drug-resistant P. aeruginosa strains. In vivo, SCM-NLC displayed significantly lower CFU/g lung than the saline and similar to that of the free SCM, even the dose in SCM-NLC group was lower than free SCM. There was not tissue damage related to the treatments. Biodistribution assessments showed a mild systemic absorption after nebulisation and a notorious absor...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 8, 2019 Category: Nanotechnology Source Type: research

Tumor selective uptake of drug-nanodiamond complexes improves therapeutic outcome in pancreatic cancer
Publication date: Available online 6 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Vijay S. Madamsetty, Anil Sharma, Maria Toma, Stefanie Samaniego, Audrey Gallud, Enfeng Wang, Krishnendu Pal, Debabrata Mukhopadhyay, Bengt FadeelAbstractPancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related deaths and novel treatment approaches are urgently needed. Here we show that poly(ethylene glycol)-functionalized nanodiamonds loaded with doxorubicin (ND-PEG-DOX) afforded a considerable improvement over free drug in an orthotopic pancreatic xenograft model. ND-PEG-DOX co...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 8, 2019 Category: Nanotechnology Source Type: research

Integrin-targeted AmpRGD sunitinib liposomes as integrated antiangiogenic tools
We report here the preparation, physico-chemical characterization, and biological evaluation of a new liposome formulation as a tool for tumor angiogenesis inhibition. Liposomes are loaded with sunitinib, a tyrosine kinase inhibitor, and decorated with cyclo-aminoprolineRGD units (cAmpRGD), efficient and selective ligands for integrin αVβ3. The RGD units play multiple roles since they target the nanovehicles at the integrin αVβ3-overexpressing cells (e.g. activated endothelial cells), favor their active cell internalization, providing drug accumulation in the cytoplasm, and likely take part in the ang...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 8, 2019 Category: Nanotechnology Source Type: research

Europium labeled lactosylated albumin as a model workflow for the development of biotherapeutics
This study introduces an original rapid protocol to allow in vitro and ex vivo evaluation of liver cells specificity of a liver diagnostic agent using delayed fluorescence of Eu labeling. Secondly, the description of the methodological approach introduces a complete workflow, including “go” and “no go” criteria relying on physical analytical methods. As such, it addresses for the clinical translation, from bench to bedside, of emerging new contrast agent and biomacromolecules. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

Phototherapy using immunologically modified carbon nanotubes to potentiate checkpoint blockade for metastatic breast cancer
Publication date: Available online 4 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Yong Li, Xiaosong Li, Austin Doughty, Connor West, Lu wang, Feifan Zhou, Robert E. Nordquist, Wei R. ChenAbstractMetastasis is the major cause of cancer-death. Checkpoint inhibition shows great promise as an immunotherapeutic treatment for cancer patients. However, most currently available checkpoint inhibitors have low response rates. To augment the antitumor efficacy of checkpoint inhibitors, such as CTLA-4 antibodies, a single-walled carbon nanotube (SWNT) modified by a novel immunoadjuvant, glycated chito...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

Chronic administration of nano-sized PAMAM dendrimers in vivo inhibits EGFR-ERK1/2-ROCK signaling pathway and attenuate diabetes-induced vascular remodeling and dysfunction
This study showed chronic in vivo administration of PAMAM dendrimers in an animal model of diabetes inhibited EGFR-ERK1/2-ROCK signaling and improved diabetes-induced vascular remodeling and dysfunction in a dose, generation (G6 > G5) and surface chemistry-dependent manner (cationic> anionic> neutral). Thus, naked PAMAM dendrimers have the propensity to modulate key (e.g. EGFR) cell signaling cascades with associated pharmacological consequences in vivo that are dependent on their physicochemical properties. PAMAMs, alone or in combination with vasculoprotective agents, might therefore have a beneficial ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

Temporal-controlled bioactive molecules releasing Core-shell Nano-system for tissue engineering strategies in endodontics
Publication date: Available online 4 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Suja Shrestha, Anil KishenAbstractTemporal-controlled release of bioactive molecules is of key importance in the clinical translation of tissue engineering techniques. We engineered a core-shell nano-system (TD-NS) that sequentially released transforming growth factor-β1 (TGF-β1), a chemotactic/proliferating growth factor and dexamethasone (Dex), an osteo/odontogenic agent in a temporal-controlled manner. In stage-1, there was a rapid release of TGF-β1, reaching a concentration of 2 ng/mL ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

Surface engineering of LENS-Ti-6Al-4 V to obtain nano- and micro- surface topography for orthopedic application
Publication date: Available online 4 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): A Revathi, Mitun Das, Vamsi K Balla, D Devika, Dwaipayan Sen, Geetha ManivasagamAbstractTwo distinct surface topographies consisting of micro- and nano- surface was developed using laser texturing (LT) and anodization process respectively and their effect on the surface-related properties of Ti-6Al-4 V fabricated using Laser Engineered Net Shaping (LENS) were determined. The topographies developed using laser texturing (25, 50 and 75% overlap) were examined using 3D profilometer, whereas, Field Emission Scann...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

HBO1 directs histone H4 specific acetylation, potentiating mechano-transduction pathways and membrane elasticity in ovarian cancer cells
This study showed that HBO1 preferentially targets histone H4 for acetylation, and has a significant involvement in the regulation of mechano-transduction pathways and cell elasticity in ovarian cancer cells. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 4, 2019 Category: Nanotechnology Source Type: research

The blood–brain barrier and beyond: Nano-based neuropharmacology and the role of extracellular matrix
Publication date: Available online 10 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Petra Henrich-Noack, Dragana Nikitovic, Monica Neagu, Anca Oana Docea, Ayse Basak Engin, Svetlana Gelperina, Michael Shtilman, Panayiotis Mitsias, George Tzanakakis, Illana Gozes, Aristidis TsatsakisAbstractRestrained drug delivery due to the blood–brain barrier (BBB) considerably limits options for the treatment of brain pathologies. The utilization of nanoparticulate (NP) carriers has been proposed as a solution. The development strategies need to address the important hurdle of NP passage across ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 4, 2019 Category: Nanotechnology Source Type: research

Immunostimulatory cytokine and doxorubicin co-loaded Nanovesicles for Cancer Immunochemotherapy
Publication date: Available online 2 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Tingting Wu, Qi Qiao, Xianya Qin, Dan Zhang, Zhiping ZhangAbstractTaking advantages of drug delivery system, immunostimulatory and chemotherapeutic agents with different physiochemical properties can be co-delivered to realize synergistic antitumor effect. Here the immunostimulatory cytokine interleukin-2 (IL-2) was firstly adsorbed in doxorubicin (DOX) loaded nanovesicles (NV-DOXIL-2) with high encapsulation efficiency by a facile solvent free method. After intravenous injection to melanoma bearing mice, NV-...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 4, 2019 Category: Nanotechnology Source Type: research

Biomedical applications of nanoflares: Targeted intracellular fluorescence probes
Publication date: Available online 28 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Karim Khanmohammadi Chenab, Reza Eivazzadeh-Keihan, Ali Maleki, Paria Pashazadeh-Panahi, Michael R Hamblin, Ahad MokhtarzadehAbstractNanoflares are intracellular probes consisting of oligonucleotides immobilized on various nanoparticles that can recognize intracellular nucleic acids or other analytes, thus releasing a fluorescent reporter dye. Single-stranded DNA (ssDNA) complementary to mRNA for a target gene is constructed containing a 3′-thiol for binding to gold nanoparticles. The ssDNA “r...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 1, 2019 Category: Nanotechnology Source Type: research

Cationic lipoplexes for treatment of cancer stem cell-derived murine lung tumors.
This study outlines the fabrication of a lipoplex of SOX2 small interfering RNA (CL-siSOX2) for targeted treatment of SOX2-enriched, CSC-derived orthotopic and xenograft lung tumors in CB-17 SCID mice. CL-siSOX2 induced tumor contraction in Cisplatin-naïve and Cisplatin-treated groups by 85% and 94% respectively. Reduction in tumor weight and volume following treatment with CL-siSOX2 was associated with reduced protein expression of SOX2 and markers of tumor initiation, inflammation, invasion and metastasis in mice tumor xenografts. And histological staining of lung tumor sections showed reduction in SOX2 expression w...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 1, 2019 Category: Nanotechnology Source Type: research

Enhanced osteoconductivity on electrically charged titanium implants treated by physicochemical surface modifications methods
This study describes the development and application of surface functionalization methods to selectively modulate surface charges and enhance implant-bone tissue integration. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 26, 2019 Category: Nanotechnology Source Type: research

Future perspectives of nanoparticle-based contrast agents for cardiac magnetic resonance in myocardial infarction
Publication date: Available online 23 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Anna Maria Grimaldi, Ernesto Forte, Teresa Infante, Carlo Cavaliere, Marco Salvatore, Filippo CademartiriAbstractCardiac Magnetic Resonance (CMR), thanks to high spatial resolution and absence of ionizing radiation, has been widely used in myocardial infarction (MI) assessment to evaluate cardiac structure, function, perfusion and viability. Nevertheless, it suffers from limitations in tissue and assessment of myocardial pathophysiological changes subsequent to MI. In this issue, nanoparticle-based contra...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 23, 2019 Category: Nanotechnology Source Type: research

Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing
Publication date: Available online 20 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jian Gao, Hui Li, Peter Torab, Kathleen E. Mach, David W. Craft, Neal J. Thomas, Chris M. Puleo, Joseph C. Liao, Tza-Huei Wang, Pak Kin WongAbstractA nanotube assisted microwave electroporation (NAME) technique is demonstrated for delivering molecular biosensors into viable bacteria for multiplex single cell pathogen identification to advance rapid diagnostics in clinical microbiology. Due to the small volume of a bacterial cell (~femtoliter), the intracellular concentration of the target molecule is high...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 20, 2019 Category: Nanotechnology Source Type: research

Nanoparticle physicochemical properties determine the activation of intracellular complement
Publication date: Available online 20 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Anna N. Ilinskaya, Ankit Shah, Alan E. Enciso, King C. Chan, Jan A. Kaczmarczyk, Josip Blonder, Eric E. Simanek, Marina A. DobrovolskaiaAbstractThe complement system plays an essential role in both innate and adaptive immunity. The traditional understanding of this system comes from studies investigating complement proteins produced by the liver and present in plasma to “complement” the immune cell-mediated response to invading pathogens. Recently, it has been reported that immune cells includ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 20, 2019 Category: Nanotechnology Source Type: research

Non-viral vectors based on cationic niosomes and minicircle DNA technology enhance gene delivery efficiency for biomedical applications in retinal disorders
Publication date: Available online 18 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Idoia Gallego, Ilia Villate-Beitia, Gema Martínez-Navarrete, Margarita Menéndez, Tania López-Méndez, Cristina Soto-Sánchez, Jon Zárate, Gustavo Puras, Eduardo Fernández, José Luis PedrazAbstractLow transfection efficiency is a major challenge to overcome in non-viral approaches to reach clinical practice. Our aim was to explore new strategies to achieve more efficient non-viral gene therapies for clinical applications and in particular, for retinal d...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 19, 2019 Category: Nanotechnology Source Type: research

Vitamin D-binding protein-loaded PLGA nanoparticles suppress Alzheimer's disease-related pathology in 5XFAD mice
Publication date: Available online 19 February 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): M.S. Seong Gak Jeon, Moon-Yong Cha, Jin-il Kim, Tae Woong Hwang, Kyoung Ah Kim, Tae Hyoung Kim, Ki Chang Song, Jwa-Jin Kim, Minho MoonAbstractThe aggregation and accumulation of amyloid beta (Aβ) peptide is believed to be the primary cause of Alzheimer's disease (AD) pathogenesis. Vitamin D-binding protein (DBP) can attenuate Aβ aggregation and accumulation. A biocompatible polymer poly (D,L-lactic acid-co-glycolic acid) (PLGA) can be loaded with therapeutic agents and control the rate of their ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 19, 2019 Category: Nanotechnology Source Type: research