An in vitro platform for elucidating the molecular genetics of S. aureus invasion of the osteocyte lacuno-canalicular network during chronic osteomyelitis
Publication date: Available online 24 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Elysia A Masters, Alec T Salminen, Stefano Begolo, Emma N Luke, Sydney C Barrett, Clyde T Overby, Ann Lindley Gill, Karen L de Mesy Bentley, Hani A Awad, Steven R Gill, Edward M Schwarz, James L McGrathAbstractStaphylococcus aureus osteomyelitis is a devasting disease that often leads to amputation. Recent findings have shown that S. aureus is capable of invading the osteocyte lacuno-canalicular network (OLCN) of cortical bone during chronic osteomyelitis. Normally a 1 μm non-motile cocci, S. aureus d...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 26, 2019 Category: Nanotechnology Source Type: research

An innovative In-situ method of creating hybrid dendrimer nano-assembly: An efficient next generation dendritic platform for drug delivery
We report an easy in-situ method of creating hybrid dendrimer nano-assembly by mixing G4.0 PAMAM (–NH2) and G3.5 PAMAM (–COONa) dendrimers with a chemotherapeutic drug docetaxel (DTX). Zeta potential, HR-TEM, 1H-NMR proved the formation of nano-assembly. In-vitro dissolution, release studies revealed pH dependent dissolution and sustained drug release. Cellular uptake, cytotoxicity, and flow cytometric analysis in human/mouse glioblastoma cells indicated the effectiveness of hybrid dendrimers. The oral administration of the hybrid dendrimers showed pharmacokinetic equivalence to intravenous injection of commerc...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 26, 2019 Category: Nanotechnology Source Type: research

Multifaceted NIR-responsive polymer-peptide-enveloped drug-loaded copper sulfide Nanoplatform for chemo-phototherapy against highly tumorigenic prostate Cancer
In this study, we constructed a dual function nanosystem, copper sulfide nanoplatform loaded with the chemotherapeutic drug docetaxel wrapped by a conjugated polymer-peptide for targeted chemo-phototherapy. The nanoconstruct has been successfully designed with a size of 186.1 ± 5.2 nm, a polydispersity index of 0.18 ± 0.01, and zeta potential of −16.4 ± 0.1 mV. The enhanced uptake and near-infrared-responsive behavior of the nanosystem resulted in efficient drug release, photothermal ablation, effective cytotoxic activity, and potentiated reactive oxygen sp...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 24, 2019 Category: Nanotechnology Source Type: research

Corrigendum to “A rationally designed photo-chemo core-shell nanomedicine for inhibiting the migration of metastatic breast cancer cells followed by photodynamic killing” [nanomedicine, 10(3):579–87]
Publication date: Available online 21 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Giridharan Loghanathan Malarvizhi, Parwathy Chandran, Archana Payickattu Retnakumari, Ranjith Ramachandran, Neha Gupta, Shantikumar Nair, Manzoor Koyakutty (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 21, 2019 Category: Nanotechnology Source Type: research

Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures
Publication date: Available online 19 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Iaroslav Gnilitskyi, Maksym Pogorielov, Roman Viter, Ana Maria Ferraria, Ana Patrícia Carapeto, Olexandr Oleshko, Leonardo Orazi, Oleg MishchenkoAbstractIn this paper, the effect of femtosecond laser nanotexturing of surfaces of Ti6Al4V and Zr implants on their biological compatibility is presented and discussed. Highly regular and homogeneous nanostructures with sub-micrometer period were imprinted on implant surfaces. Surfaces were morphologically and chemically investigated by SEM and XPS. HDFa cell...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 20, 2019 Category: Nanotechnology Source Type: research

Regeneration of large bone defects using mesoporous silica coated magnetic nanoparticles during distraction osteogenesis
This study demonstrates the notable potential of M-MSNs in promoting bone regeneration during DO by enhancing the osteogenic differentiation of MSCs, paving the way for clinical translation of M-MSNs in DO to repair large bone defects.Graphical AbstractTranslational potential of mesoporous silica coated iron oxide nanoparticles (M-MSNs) in regenerating long bone defects during distraction osteogenesis. Local injection of M-MSNs to the distraction gap promotes new bone formation and consolidation through enhancing the osteogenic differentiation of mesenchymal stem cells via the Wnt/β-catenin signaling pathway. (Source:...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 20, 2019 Category: Nanotechnology Source Type: research

Time- and dose-dependent gene expression analysis of macrophage response as a function of porosity of silica nanoparticles
In this study, RAW264.7 macrophages were treated with spherical nonporous and mesoporous silica nanoparticles of similar size at different incubation times and concentrations. RNA-sequencing was used to study transcriptional profiles. Bioinformatics analyses, functional annotation clustering, and network analyses were employed to understand signaling pathways of cellular response as a function of porosity, incubation time, and concentration. Porosity introduced drastic changes to the genomic response of macrophages at equitoxic concentrations and incubation times. Direct relations between increases in time and concentratio...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 20, 2019 Category: Nanotechnology Source Type: research

Structural and positional impact on DNAzyme-based electrochemical sensors for metal ions
Publication date: Available online 19 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xudong Guo, Min Li, Rongtao Zhao, Yi Yang, Ruili Wang, Feng Wu, Leili Jia, Yuxi Zhang, Lihua Wang, Zhibei Qu, Fei Wang, Ying Zhu, Rongzhang Hao, Xueli Zhang, Hongbin SongAbstractThe rapid, accurate and convenient detection of heavy metal is very important to public health. Here, we developed a DNAzyme-based electrochemical sensor for Pb2+. A DNAzyme-including and Pb2+ active probe was anchored to the biosensing interface, based on the well-defined self-assembled, three-dimensional DNA nanostructure. The resul...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 20, 2019 Category: Nanotechnology Source Type: research

Spatial manipulation of magnetically-responsive nanoparticle engineered human neuronal progenitor cells
Publication date: Available online 17 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ivan Guryanov, Ekaterina Naumenko, Svetlana Konnova, Maria Lagarkova, Sergey Kiselev, Rawil FakhrullinAbstractHere we report a detailed investigation of the interaction of neuronal progenitor cells and neurons with polyelectrolyte-stabilized magnetic iron oxide nanoparticles. Human neuronal progenitor and neurons were differentiated in vitro from fibroblast-derived induced pluripotent stem cells. The cytotoxic effects of poly(allylamine hydrochloride) was determined on human skin fibroblasts and neuronal prog...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 18, 2019 Category: Nanotechnology Source Type: research

Exploring the interactions between engineered nanomaterials and immune cells at 3D Nano-bio interfaces to discover potent Nano-adjuvants
Publication date: Available online 18 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ronglin Ma, Huizhen Zheng, Qi Liu, Di Wu, Wei Li, Shujuan Xu, Xiaoming Cai, Ruibin LiAbstractEngineered nanomaterials (ENMs) as adjuvants can potentiate the adaptive immune responses to antigens by activating immune cells in three dimensional (3D) matrixes of tissues. However, few reports explored the interactions of nano-adjuvants and immune cells at 3D nano-bio interfaces. Herein we designed an alginate-calcium microsphere of macrophage cells to explore the interactions. By an extensive comparison of ENM-in...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 18, 2019 Category: Nanotechnology Source Type: research

Tumor immune microenvironment and nano-immunotherapeutics in colorectal cancer
Publication date: Available online 15 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Yang Xiong, Ying Wang, Karthik TiruthaniAbstractColorectal cancer (CRC) is predicted to be the 2nd leading cause of cancer-related death in United States in 2019. Immunotherapies such as checkpoint inhibitors have proven efficacy in patients with high level of microsatellite instability and refractory to routine chemotherapy. Despite this, immunotherapy-based treatment is seriously limited by cancer immunogenicity which are evolved to evade immune surveillance in many circumstances. Efforts are made by resear...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 15, 2019 Category: Nanotechnology Source Type: research

Alum as an adjuvant for nanoparticle based vaccines: A case study with a hybrid nanoparticle-based nicotine vaccine
In this study, we attempted to improve the immunological performance of a hybrid nanoparticle-based nicotine vaccine (NanoNicVac) by co-administering it with Alum. It was found that Alum severely restricted the release of NanoNicVac at the site of injection. Moreover, Alum damaged the hybrid structure of the vaccine. In the animal trial, mice immunized with NanoNicVac alone achieved an anti-nicotine IgG titer of 3.5 ± 0.2 × 104 after three injections. Unexpectedly, Alum with quantities of 125, 250, 500, and 1000 μg did not enhance the immunogenicity of NanoNicVac. In addition, Alum ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 11, 2019 Category: Nanotechnology Source Type: research

Targeting macrophages and their recruitment in the oral cavity using swellable (+) alpha tocopheryl phosphate nanostructures
Publication date: Available online 11 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Robert A. Harper, Latrisha Petersen, Mais Saleh, Gordon B. Proctor, Guy H. Carpenter, Robert Gambogi, Robert Hider, Stuart A. JonesAbstractThe phosphorylation of (+) alpha tocopherol produces adhesive nanostructures that interact with oral biofilms to restrict their growth. The aim of this work was to understand if these adhesive (+) alpha tocopheryl phosphate (α-TP) nanostructures could also control macrophage responses to the presence of oral bacteria. The (+) α-TP planar bilayer fragments (175&...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 11, 2019 Category: Nanotechnology Source Type: research

Leveraging surface Plasmon resonance to dissect the interfacial properties of nanoparticles: Implications for tissue binding and tumor penetration
Publication date: Available online 6 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Aniket S. Wadajkar, Jimena G. Dancy, Christine P. Carney, Brian S. Hampton, Heather M. Ames, Jeffrey A. Winkles, Graeme F. Woodworth, Anthony J. KimAbstractTherapeutic efficacy of nanoparticle-drug formulations for cancer applications is significantly impacted by the extent of intra-tumoral accumulation and tumor tissue penetration. We advanced the application of surface plasmon resonance to examine interfacial properties of various clinical and emerging nanoparticles related to tumor tissue penetration. We ob...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 7, 2019 Category: Nanotechnology Source Type: research

Optimization of sonic hedgehog delivery to the penis from self-assembling nanofiber hydrogels to preserve penile morphology after cavernous nerve injury
In this study we optimize the animal model, SHH concentration, duration of suppression, and location of delivery, to maximize SM preservation. SHH treatment suppressed apoptosis and preserved SM 48%. Increased SHH duration preserved SM 100%. Simultaneous penis/CN delivery increased SM 127%. Optimization of SHH PA delivery is essential for clinical translation to ED patients, and the PA vehicle has wide applicability as an in vivo delivery tool.Graphical AbstractThese studies show that Sonic hedgehog treatment of the penis and cavernous nerve by peptide amphiphile nanofiber hydrogel reduces apoptosis, preserves smooth muscl...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 5, 2019 Category: Nanotechnology Source Type: research

Development of Halobetasol-loaded nanostructured lipid carrier for dermal administration: Optimization, physicochemical and biopharmaceutical behavior, and therapeutic efficacy
Publication date: Available online 4 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Paulina Carvajal-Vidal, María-José Fábrega, Marta Espina, Ana Cristina Calpena, M. Luisa GarcíaAbstractHalobetasol propionate (HB) is considered a super potent drug in the group of topical corticosteroids. HB has anti-inflammatory activity, vasoconstriction properties, and due to its high skin penetration, it can cause systemic side effects. To improve its characteristics, enhance topical effectiveness and reduce penetration to systemic circulation, a study to optimize and character...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 4, 2019 Category: Nanotechnology Source Type: research

Next-generation paclitaxel-nanoparticle formulation for pancreatic cancer treatment
In this study, we demonstrate the therapeutic efficacy of a novel PLGA-PTX nanoformulation (PPNPs). This system shows efficacy both in vitro and in vivo. Specifically, nanoindentation studies show an increased rigidity of PPNPs-treated pancreatic cancer cells, indicating reduced metastatic potential as confirmed with additional results herein (proliferation, colony formation, invasion, migration, cell cycle arrest, apoptosis induction). In vivo results indicate successful reduction in tumor growth, metastasis, and EMT markers in chemo-naïve and chemo-exposed orthotopic xenograft mice, suggesting that PPNPs may be a ne...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 4, 2019 Category: Nanotechnology Source Type: research

Development of medical-grade, discrete, multi-walled carbon nanotubes as drug delivery molecules to enhance the treatment of hematological malignancies
Publication date: Available online 4 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Carolyne Falank, Aaron W. Tasset, Mariah Farrell, Sophie Harris, Paul Everill, Milos Marinkovic, Michaela R. ReaganAbstractCarbon nanotubes (CNTs) hold great potential as drug delivery transporters given their large drug-binding surface area. Herein, we designed novel, multi-walled, discrete CNTs (dMWCNTs), PEGylated dMWCNTs (PEG-dMWCNTs), and bone-targeting (BT), alendronate-conjugated PEG-dMWCNTs (BT-PEG-dMWCNTs). Using zeta potential, thermogravimetric analysis, TEM, SEM, and FTIR, dMWCNTs were characterize...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 4, 2019 Category: Nanotechnology Source Type: research

Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5
Publication date: Available online 4 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Sylvia N. Kłodzińska, Daniel Pletzer, Negin Rahanjam, Thomas Rades, Robert E.W. Hancock, Hanne M. NielsenAbstractAnti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33–60% of peptide were create...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 4, 2019 Category: Nanotechnology Source Type: research

Hydrothermal treatment of etched titanium: A potential surface nano-modification technique for enhanced biocompatibility
In conclusion, hydrothermal oxidation on an etched Ti surface can generate characteristics that have excellent prospects for the biomedical field.Graphical AbstractNanomorphologies such as nanoflowers, nanopetals and nanofibers were successfully fabricated on an etched titanium surface by hydrothermal treatment. The performed hydrothermal treatment is effective in terms of biocompatibility with cytocompatibility, mineralization and antibacterial activity. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 1, 2019 Category: Nanotechnology Source Type: research

Interfering with endolysosomal trafficking enhances release of bioactive exosomes
Publication date: Available online 30 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Francisco G. Ortega, Marieke T. Roefs, Diego de Miguel Perez, Sander A. Kooijmans, Olivier G. de Jong, Joost P. Sluijter, Raymond M. Schiffelers, Pieter VaderAbstractExosomes are cell-derived extracellular vesicles of 30–150 nm in size and are involved in intercellular communication. Because of their bioactive cargo, consisting of proteins, RNA and lipids, and their natural ability to deliver these biomolecules to recipient cells, exosomes are increasingly being studied as novel drug delivery vehicl...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 30, 2019 Category: Nanotechnology Source Type: research

A glutathione responsive nitric oxide release system based on charge-reversal chitosan nanoparticles for enhancing synergistic effect against multidrug resistance tumor
Publication date: Available online 29 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiaoyan Niu, Jing Cao, Yapei Zhang, Xuefeng Gao, Mingbo Cheng, Yang Liu, Wei Wang, Zhi YuanAbstractThe combination therapy of nitric oxide (NO) and anticancer drug was developed for reversing multidrug resistance (MDR). In order to avoid NO release during the blood circulation, and realize pinpointed release in the tumor cells, we designed a tumor-specific NO-release system based on 10-hydroxycamptothecin (HCPT)-loaded charge-reversal chitosan nanoparticles and covalently linked phenylsulfonyl furoxan (glutath...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 29, 2019 Category: Nanotechnology Source Type: research

Oral pH sensitive GNS@ab Nanoprobes for targeted therapy of Helicobacter Pylori without disturbance gut microbiome
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiao Zhi, Yanlei Liu, Lingnan Lin, Meng Yang, Lingxia Zhang, Li Zhang, Yangtian Liu, Gabriel Alfranca, Lijun Ma, Qian Zhang, Hualin Fu, João Conde, Xianting Di, Jian Ni, Jie Song, Daxiang CuiAbstractHow to eradicate Helicobacter pylori (H. pylori) in vivo with antibiotic resistance owns tremendous clinical requirement. Herein, gold nanostars were conjugated with acid-sensitive cis-aconitic anhydride modified anti-H. pylori polyclonal antibodies, resultant pH sensitive gold nanostars@H. pylori-antibodies...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 23, 2019 Category: Nanotechnology Source Type: research

Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiangshi Sun, Shiyan Dong, Xiangyu Li, Kongtong Yu, Fengying Sun, Robert J. Lee, Youxin Li, Lesheng TengAbstractSystemic delivery of siRNA to target tissues is difficult to achieve owing to its limited cellular uptake and poor serum stability. Herein, polymeric nanoparticles were developed for systemic administration of siRNA to inflamed tissues. The polymeric nanoparticles were composed of PK3 as a pH-sensitive polymer, folate-polyethyleneglycol-poly(lactide-co-glycolide) as a targeting ligand, and a DOTAP/si...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 23, 2019 Category: Nanotechnology Source Type: research

Targeted laser therapy synergistically enhances efficacy of antibiotics against multi-drug resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Dickson K. Kirui, Gregor Weber, Jennifer Talackine, Nancy J. MillenbaughAbstractThe growing prevalence of biofilm-associated multi-drug resistant (MDR) bacteria necessitates the innovation of non-traditional approaches to improve the effectiveness of mainstay antibiotics. Here, we evaluated the use of gold nanoparticle (GNP)-targeted pulsed laser therapy to enhance antibiotic efficacy against in vitro methicillin-resistant Staphylococcus aureus (MRSA) and MDR Pseudomonas aeruginosa biofilms. Treatment with ant...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 22, 2019 Category: Nanotechnology Source Type: research

NIR-responsive Nanoplatform for pre/intraoperative image-guided carcinoma surgery and Photothermal ablation of residual tumor tissue
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Jiao Song, Liang Zhang, Hengjing Yi, Ju Huang, Ni Zhang, Yixin Zhong, Lan Hao, KeYang, Zhigang Wang, Dong Wang, Zhu YangAbstractLocal recurrence is common among patients with advanced cancer who have undergone surgery. Here, we developed a new surgical treatment for cancer based on a nanoparticle that loaded a near-infrared dye (IR780 iodide) and perfluorooctyl bromide into liposomes (NP-IR780). In an orthotopic breast cancer mouse model, NP-IR780 was demonstrated to have excellent tumor-targeting ability due ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 22, 2019 Category: Nanotechnology Source Type: research

pH and redox dual responsive carrier-free anticancer drug nanoparticles for targeted delivery and synergistic therapy
Publication date: Available online 20 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mengjiao Zhou, Weijia Wei, Xianfeng Chen, Xiuzhen Xu, Xiaohong Zhang, Xiujuan ZhangAbstractAdvanced drug delivery systems often employ nanomaterials as carriers to deliver drugs to desirable disease sites for enhanced efficacy. However, most systems have low drug loading capacity and cause safety concerns. Therefore, many anticancer therapeutics have recently been assembled to NPs form without using any additional nanocarrier to achieve high drug loading. However, carrier-free nanomedicines are often constrain...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 21, 2019 Category: Nanotechnology Source Type: research

Gold Nanoclusters as a contrast agent for image-guided surgery of head and neck tumors
Publication date: Available online 17 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Cindy Colombé, Xavier Le Guével, Angela Martin-Serrano, Maxime Henry, Estelle Porret, Clothilde Comby-Zerbino, Rodolphe Antoine, Ihab Atallah, Benoit Busser, Jean-Luc Coll, Christian Adrien Righini, Lucie SanceyAbstractWith the objective to evaluate the potential of ultra-small gold (Au) nanoclusters (NCs) for optical image-guided surgery, we synthesized and characterized AuNCs shelled by zwitterionic or pegylated ligands. The toxicity of the different AuNCs was evaluated on the Head and Neck Squ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 18, 2019 Category: Nanotechnology Source Type: research

Preparation and characterization of nanoliposomal bortezomib formulations and evaluation of their anti-cancer efficacy in mice bearing C26 colon carcinoma and B16F0 melanoma
Publication date: Available online 16 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mitra Korani, Solmaz Ghaffari, Hossein Attar, Mohammad Mashreghi, Mahmoud Reza JaafariAbstractThree Bortezomib (BTZ) liposomal formulations including of HSPC/Cholesterol/DSPE-mPEG2000 (F1), HSPC/DSPG/Cholesterol (F2), and HSPC/DSPG/Cholesterol/DSPE-mPEG2000 (F3) were prepared and characterized. Results demonstrated that the size of formulations ranged 72–92 nm. The DSPE-mPEG2000 containing formulations (F1 and F3) had higher BTZ encapsulation compared to F2 formulation. The size of the liposomal for...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 17, 2019 Category: Nanotechnology Source Type: research

Anti-biofilm activity of garlic extract loaded nanoparticles
This study describes a sol–gel based nanoparticle system loaded with garlic extract (GE-np) that exhibits: i) slow and sustained release of garlic components; ii) stabilization of the active components; and iii) significant enhancement of antimicrobial and antibiofilm activity relative to the free garlic extract. Also, GE-np were efficient in penetrating and disrupting the well-established methicillin-resistant Staphylococcus aureus (MRSA) biofilms. Overall, the study suggests that GE-np might be a promising candidate for the treatment of chronic infections due to biofilm forming drug-resistant bacteria.Graphical Abs...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 12, 2019 Category: Nanotechnology Source Type: research

Knee osteoarthritis grading by resonant Raman and surface-enhanced Raman scattering (SERS) analysis of synovial fluid
This study demonstrates that combined Raman and SERS spectra of synovial fluid enables an efficient grading of knee osteoarthritis based on the carotenoid and proteomic contents of synovial fluid. The turnaround time of the assay is around 10 minutes per sample, making it amenable to implementation in the point-of-care setting for aiding the diagnosis and grading of osteoarthritis. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 12, 2019 Category: Nanotechnology Source Type: research

Silencing AXL by covalent siRNA-gelatin-antibody nanoconjugate inactivates mTOR/EMT pathway and stimulates p53 for TKI sensitization in NSCLC
Publication date: Available online 12 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Dhananjay Suresh, Ajit Zambre, Soumavo Mukherjee, Shreya Ghoshdastidar, Yuexu Jiang, Trupti Joshi, Anandhi Upendran, Raghuraman KannanAbstractNon-small cell lung cancer (NSCLC) is the leading cause of cancer mortality with the 5-year survival rate remains a dismal 16% for the past 40 years. Drug resistance is a major obstacle to achieving long-term patient survival. Identifying and validating molecular biomarkers responsible for resistance and thereby adopting multi-directional therapy is necessary to imp...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 12, 2019 Category: Nanotechnology Source Type: research

Multilayer nanoscale functionalization to treat disorders and enhance regeneration of bone tissue
Publication date: Available online 17 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ana Marina Ferreira, Chiara Tonda-Turo, Elena Mancuso, Piergiorgio GentileAbstractThe coatings application onto medical devices has experienced a continuous growth in the last few years. Medical device coating market is expected to grow at a CAGR of 5.16% to reach USD 10 million by 2023 due to the increasing geriatric population and the growing demand for continuous innovation. Layer-by-Layer (LbL) assembly represents a versatile method to modify the surface properties, in order to control cell interaction a...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 9, 2019 Category: Nanotechnology Source Type: research

A traceable nanoplatform for enhanced chemo-photodynamic therapy by reducing oxygen consumption
Publication date: Available online 6 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Hanchun Yao, Suge Zhang, Xiaofang Guo, Ying Li, Jinjin Ren, Huijuan Zhou, Bin Du, Jie ZhouAbstractTumor hypoxia impedes the efficiencies of oxygen-dependent photodynamic therapy (PDT) and chemotherapy. Herein, we design a traceable nanoplatform (DOX/Met/BSA-HA-CDs) by reducing oxygen (O2) consumption to overcome the hypoxia-caused cancer therapy. Carbon dots (CDs) are used not only as a PDT agent but also applied for in vivo traceable imaging. Metformin (Met), a potent antihyperglycaemic agent, to improve tumor...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 8, 2019 Category: Nanotechnology Source Type: research

Combining magnetic nanoparticles and icosahedral boron clusters in biocompatible inorganic Nanohybrids for Cancer therapy
Publication date: Available online 3 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Elena Oleshkevich, Anna Morancho, Arpita Saha, Koen M.O. Galenkamp, Alba Grayston, Simonetta Geninatti Crich, Diego Alberti, Nicoletta Protti, Joan X. Comella, Francesc Teixidor, Anna Rosell, Clara ViñasAbstractThe potential biomedical applications of the MNPs nanohybrids coated with m-carboranylphosphinate (1-MNPs) as a theranostic biomaterial for cancer therapy were tested. The cellular uptake and toxicity profile of 1-MNPs from culture media by human brain endothelial cells (hCMEC/D3) and glioblastoma...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 4, 2019 Category: Nanotechnology Source Type: research

Therapeutic potential of andrographolide-loaded nanoparticles on a murine asthma model
In conclusion, AGNP effectively controlled mild and severe asthma and the pulmonary administration of AGNP was more efficacious than the oral route.Graphical AbstractAndrographolide loaded nanoparticles successfully suppressed inflammatory mediators associated with ovalbumin induced asthma in mice, where the efficacy of nanoparticle was found to be better in pulmonary administration than its oral administration. The formulation showed improved bioavailability than the free drug. Different pharmacokinetic parameters and biodistribution of andrographolide in plasma and lung was determined by LC–MS/MS and radiolabeling ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 4, 2019 Category: Nanotechnology Source Type: research

Daunorubicin-containing CLL1-targeting nanomicelles have anti-leukemia stem cell activity in acute myeloid leukemia
Publication date: Available online 2 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Tzu-yin Lin, Yanjun Zhu, Yuanpei Li, Hongyong Zhang, Ai-Hong Ma, Qilai Long, James Keck, Kit S. Lam, Chong-xian Pan, Brian A. JonasAbstractPatients with acute myeloid leukemia have a very poor prognosis related to a high rate of relapse and drug-related toxicity. The ability of leukemia stem cells (LSCs) to survive chemotherapy is primarily responsible for relapse, and eliminating LSCs is ultimately essential for cure. We developed novel disulfide-crosslinked CLL1-targeting micelles (DC-CTM), which can deliver ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 3, 2019 Category: Nanotechnology Source Type: research

Triple-hit therapeutic approach for triple negative breast cancers using docetaxel nanoparticles, EN1-iPeps and RGD peptides
Publication date: Available online 2 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Anabel Sorolla, Edina Wang, Tristan D Clemons, Cameron W Evans, Janice HC Plani-Lam, Emily Golden, Ben Dessauvagie, Andrew D Redfern, K. Swaminathan-Iyer, Pilar BlancafortAbstractTriple negative breast cancers (TNBC) are aggressive malignancies for which chemotherapy is the only treatment option. Many TNBC acquire chemotherapy resistance, notably docetaxel, which has been associated with the overexpression of transcription factors (TFs), such as ENGRAILED1 (EN1). Here, we have developed a tumor delivery system ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 3, 2019 Category: Nanotechnology Source Type: research

Subtle changes in surface-tethered groups on PEGylated DNA nanoparticles significantly influence gene transfection and cellular uptake
This study identifies a new parameter for the engineering design of PEGylated DNA NPs.Graphical AbstractThe effect of PEG terminal groups in the interactions of PEGylated DNA nanoparticles with cells is investigated through an integrated experimental and computational approach. For nanoparticles with alkyl/hydroxyalkyl PEG terminal group, the highest transfection efficiency and cellular uptake levels occur at intermediate alkyl chain length. Subtle changes in PEG terminal groups significantly affect gene expression and cellular uptake levels. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 30, 2019 Category: Nanotechnology Source Type: research

Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX
We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470–490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 30, 2019 Category: Nanotechnology Source Type: research

A photo-triggered conjugation approach for attaching RGD ligands to biodegradable mesoporous silica nanoparticles for the tumor fluorescent imaging
Publication date: Available online 29 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xinyi Xu, Hanrui Li, Ke Li, Qi Zeng, Yin Liu, Yun Zeng, Dan Chen, Jimin Liang, Xueli Chen, Yonghua ZhanAbstractFluorescent probes conjugated to peptide or antibody directing groups, that exhibit high signal to background ratios, have been widely used to image tumors and monitor their growth. A photo-triggered cycloaddition reaction between the arginine-glycine-aspartic acid -N-ɛ-acryllysine (RGD-Acrk) peptides and the tetrazole compounds bound to the surface of biodegradable mesoporous silica nanoparticles ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 30, 2019 Category: Nanotechnology Source Type: research

An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery
Publication date: Available online 25 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ci Ren, Xiaomin Li, Lu Mao, Jinfeng Xiong, Chun Gao, Hui Shen, Liming Wang, Da Zhu, Wencheng Ding, Hui WangAbstractGene therapy targeted human papillomavirus (HPV) is a promising treatment for cervical cancer, and the key for clinical application depends on an effective gene delivery method. Our aim was to formulate a new pharmaceutical formula for appropriate gene delivery intravaginally. For the first time, we here developed a new polyethylenimine (PEI) based vaginal suppository. The sectional immunofluore...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 25, 2019 Category: Nanotechnology Source Type: research

Scavenger receptor-AI-targeted ultrasmall gold nanoclusters facilitate in vivo MR and ex vivo fluorescence dual-modality visualization of vulnerable atherosclerotic plaques
In this study, Au3+ was intensely clustered into the scaffold GSH, Gd-DTPA was chelated to create MR imaging capacity and PP1 was conjugated to obtain active targeting to SR-AI over-expressed foam macrophages. The resulting PP1-Au@GSH@Gd NCs had a sufficient load of Au and Gd species, which was rationale for the significantly improved fluorescence and relaxivity values. The prominent MR/fluorescence dual-modality imaging abilities, augmented accumulation in vulnerable plaques and admirable biocompatibility make this noninvasive molecular strategy a favorable choice of bio-imaging evaluations for atherosclerotic plaques. (S...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 25, 2019 Category: Nanotechnology Source Type: research

O2-evolving and ROS-Activable nanoparticles for treatment of multi-drug resistant Cancer by combination of photodynamic therapy and chemotherapy
Publication date: Available online 25 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Zhijian Luo, Mingxing Li, Meiling Zhou, Huayun Li, Youyou Chen, Xiaoqun Ren, Yan DaiAbstractHerein, a novel nano-system IF7-ROSPCNP, which is O2-evolving and reactive oxygen species (ROS)-activable, was developed for enhancing the combination chemotherapy and photodynamic therapy (PDT). The nanoparticles composed of photosensitizers (disulfonated meso-tetraphenylporphine, TPPS2a) and catalase in the inner core, doxorubicin (DOX) in the polymeric shell and functionalized on its surface with IF7 peptide, which...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 25, 2019 Category: Nanotechnology Source Type: research

Delivery of biologically active miR-34a in Normal and Cancer mammary epithelial cells by synthetic nanoparticles
In conclusion, our studies suggest that silica nanoparticles can mediate the delivery of miR-34a directly into mammary tumors while preserving its molecular and biological activity.Graphical AbstractmiR-34a is efficiently delivered by SiO2 nanoparticles and it inhibits tumor growth. (Source: Nanomedicine: Nanotechnology, Biology and Medicine)
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 25, 2019 Category: Nanotechnology Source Type: research

Ferritin-based anticancer metallodrug delivery: Crystallographic, analytical and cytotoxicity studies
Publication date: Available online 25 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): D.M. Monti, G. Ferraro, A. MerlinoAbstractThe encapsulation of anticancer metal-based drugs within a protein nanocage represents a valuable strategy to improve the efficacy and selectivity of these compounds towards cancer cells. The preparation, characterization of the in vitro cytotoxicity and X-ray structures of several ferritin-metallodrug nanocomposites (mainly containing platinum-, ruthenium- and gold-based anticancer agents) are here reviewed. The molecular mechanisms of action of these Ft-metallodrug...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 25, 2019 Category: Nanotechnology Source Type: research

Harnessing the versatility of PLGA nanoparticles for targeted Cre-mediated recombination
Publication date: Available online 23 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ngoc B. Nguyen, Cheng-Han Chen, Yulong Zhang, Peng Zhao, Benjamin Wu, Reza ArdehaliAbstractLigand-dependent Cre recombinases are pivotal tools for the generation of inducible somatic mutants. This method enables spatial and temporal control of gene activity through tamoxifen administration, providing new avenues for studying gene function and establishing animal models of human diseases. While this paved the way for developmental studies previously deemed impractical, the generation of tissue-specific transg...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 24, 2019 Category: Nanotechnology Source Type: research

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer
Publication date: Available online 22 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Adel Al Fatease, Vidhi Shah, Duc X. Nguyen, Brianna Cote, Nicole LeBlanc, Deepa A. Rao, Adam WG AlaniAbstractThis work looks to improve the efficacy of Adriamycin (ADR) while mitigating its cardiotoxicity using combinations of micellar resveratrol (R): quercetin (Q) (mRQ) or R: curcumin (C) (mRC) in healthy mice and ovarian cancer xenograft models. Ovarian cancer cells, ES2-Luc, or A2780ADR are inoculated in mice (n = 4/group) and sorted into eight cohorts. Mice are treated weekly for 4 weeks ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 24, 2019 Category: Nanotechnology Source Type: research

Tumor-targeted drug delivery and sensitization by MMP2-responsive polymeric micelles
In this study, the micelles assembled by a matrix metalloproteinase 2 (MMP2)-sensitive self-assembling efflux inhibitor (PEG2k-pp-PE) were developed and evaluated in various cancer models. In vitro, the PEG2k-pp-PE micelles enhanced the cellular uptake and tissue penetration and sensitized the cancers to drug treatments in MDR cancer cells and their three-dimensional multicellular spheroids. Their efflux inhibitory capability was comparable to those of the well-known small-molecule P-glycoprotein (P-gp) inhibitor and polymeric P-gp inhibitor. In vivo, the PEG2k-pp-PE micelles could specifically and effectively deliver the ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 18, 2019 Category: Nanotechnology Source Type: research

Nanoemulsion strategy of pioglitazone for the treatment of skin inflammatory diseases
Publication date: Available online 18 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Lupe C. Espinoza, Marcelle Silva-Abreu, Ana C. Calpena, María J. Rodríguez-Lagunas, María-José Fábrega, María L. Garduño-Ramírez, Beatriz ClaresAbstractPioglitazone (PGZ) is a peroxisome proliferator-activated receptor agonist. Its role in the inflammatory response modulation opens the door for additional therapeutic applications. The purpose of this study was to develop a pioglitazone nanoemulsion (PGZ-NE) to investigate its anti-inflammatory efficacy ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 18, 2019 Category: Nanotechnology Source Type: research