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Source: GEO: Gene Expression Omnibus
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Total 772 results found since Jan 2013.
GSE222695 Transcriptomic profiling of CDCP1 knock-down in human cardiac fibroblasts (HCFs)
Contributors : Vishakantha Murthy ; Thanh L Nguyen ; Duan LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the function of CDCP1 in regulation of HCF proliferation, we cultured primary HCFs and transfected with non-targeting siRNA and CDCP1 siRNAs. Total RNA samples were extracted for RNA-seq to identify transcriptome-wide gene expression changes in the HCFs after CDCP1 knock-down (KD).
Source: GEO: Gene Expression Omnibus - September 22, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE222993 The effect of PLVAP knockdown in human liver sinusoidal endothelial cells (LSECs).
Contributors : Alex Wilkinson ; Paul Horn ; Daniel Patten ; Shishir ShettySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the phenotypic consequences of PLVAP presence/absence in human LSEC, we utilised siRNA knockdown in 3 independent LSEC donors.We then performed gene expression profiling analysis using data obtained from RNA-seq of LSEC with siRNA knockdown of PLVAP and siRNA Negative Control cells.
Source: GEO: Gene Expression Omnibus - September 15, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE242834 Gene expression profiling and the effect of HDAC9 in cisplatin resistance of esophageal squamous cell carcinoma
Series Type : Non-coding RNA profiling by arrayOrganism : Homo sapiensEsophageal cancer is one of the most serious health problems globally because of its high incidence and mortality rate. Cisplatin(CDDP) is the primary treatment but limited by the drug resistance. However, the mechanism of cisplatin resistance remains unclear. Herein, the CDDP-resistant cell line TE-1/CDDP was established to compared with TE-1, the expression profiles of circRNAs were compared between TE-1/CDDP and TE-1 using microarray analysis, Histone deacetylase 9(HDAC9) was identified significantly up-regulated. Using siRNA and conducting cell funct...
Source: GEO: Gene Expression Omnibus - September 14, 2023 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by array Homo sapiens Source Type: research
GSE219028 Investigating TIAM1 in NSCLC (RNA-Seq)
Contributors : Joe Maltas ; Angeliki MalliriSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe T lymphoma invasion and metastasis inducing protein 1 (TIAM1) is a guanine nucleotide exchange factor (GEF) that activates the small GTPase RAC1. We have shown that nuclear TIAM1promotes the migration of NSCLC cells. To investigate the requirement for TIAM1 in regulating gene expression of NSCLC cells, we performed RNA-seq on H441 cells transfected with either siRNA for TIAM1 (siTIAM1) or a control siRNA (siControl) to identify differentially expressed genes.
Source: GEO: Gene Expression Omnibus - September 12, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE242255 DNA-PKcs is a key transcriptional and splicing regulator of AR-Vs in advanced prostate cancer
Contributors : Luke Gaughan ; Beth Adamson ; Nicholas BrittainSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the role of DNA-PKcs and RBMX in the regulation of androgen receptor variants we used the CWR22Rv1-AR-EK and CWR22Rv1 cell line in which DNA-PKcs or RBMX has been knocked down by siRNA or DNA-PKcs was inhibited with 1 µM NU7441, NU5455 or AZD7648. We then performed gene expression analysis using data obtained from RNA-seq at two time points (72 hours knockdown and 24 hours drug treatment).
Source: GEO: Gene Expression Omnibus - September 11, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE103987 MAP3K8 is a major regulator of inflammation in human airway epithelial cells
Contributors : Chih-Yung Chiu ; Saffron A Willis-Owen ; Kenny C Wong ; Stuart N Farrow ; Youming Zhang ; Miriam F Moffatt ; William O CooksonSeries Type : Expression profiling by arrayOrganism : Homo sapiensIn this experiment we catalogue temporal variation in transcription occurring during IL1B stimulation under control (non-targeting siRNA control) conditions and partial MAP3K8 siRNA knockdown in the A549 cell line. We measure global gene transcription at 0, 1, 2, 4, 6, 8, and 12 hours using Affymetrix HuGene 1.1 ST array. Nineteen genes demonstrated a distinct temporal response to Il1B stimulation following suppression of MAP3K8.
Source: GEO: Gene Expression Omnibus - September 9, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE221308 Xbp1 knockdown in CHO cells stably expressing Cdh13 (RNA-Seq)
Contributors : Shunbun Kita ; Keita FukuokaSeries Type : Expression profiling by high throughput sequencingOrganism : Cricetulus griseusRNA expressions of CHO cells stably expressing Cdh13 were compared between cells transfected with negative control siRNA and with Xbp1 siRNA for 48 hours.
Source: GEO: Gene Expression Omnibus - September 6, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Cricetulus griseus Source Type: research
GSE198359 RNA-seq analysis reveals potential molecular mechanism of RAD54B on cell proliferation of inflamed human dental pulp cells
Conclusions: Gene expression profiles of normal, carious and pulpitis human dental pulp tissues were revealed. HRR components was elucidated to function in dental pulp inflammation. Among HRR DEGs, RAD54B could regulate the cell proliferation of inflamed hDPCs via P53/P21 signaling. This research not only deepens our understanding of dental pulp inflammation but also provides a new insight to clarify the underlying mechanisms.
Source: GEO: Gene Expression Omnibus - September 2, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE180610 Differential gene expression in MEF SEC24D knockout cells with additional SEC24s knockdown
Contributors : Jinoh Kim ; Chung-Ling LuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe transfected MEF SEC24D KO cells with SEC24B siRNA for a double KD or combined SEC24s siRNA (SEC24a, 24B and 24C) for a quadruple KD. Cells were transfected with siRNA or Scramble siRNA (control) for 2 days then total RNA for each knockdown sample was extracted and purified for RNA-seq to evaluate the change in transcriptome.
Source: GEO: Gene Expression Omnibus - September 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE164330 Examining SRP pathway function in mRNA localization to the endoplasmic reticulum
Contributors : Jessica R Child ; Alex C Hofler ; Qiang Chen ; Brenda H Yang ; JohnCarlo Kristofich ; Tianli Zheng ; Molly M Hannigan ; Andew L Elles ; Christopher V NicchittaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSignal recognition particle (SRP) pathway function in secretory/membrane protein translocation across the endoplasmic reticulum (ER) is well-established; its role in mRNA localization to the ER in mammalian cells remains largely unexplored. We address this question in SRP receptor (SR) knockout mammalian cell lines. SRPRB KO cells were generated by CRISPR editing. SR...
Source: GEO: Gene Expression Omnibus - September 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE197092 RNAseq analysis of genes differentially expressed in SMMC7721 cells with SNRPE knockdown
Contributors : Qipeng Wu ; Zhenzhou Jiang ; Luyong ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHepatocellular carcinoma (HCC) is a malignant tumor with poor prognosis and lack of effective treatment targets is the major challenge in treating HCC. Previous studies have revealed significant changes in spliceosome-related genes in HCC. The unique expression pattern in tumor cells makes oncofetal proteins ideal candidate targets for cancer diagnosis and treatment. Therefore, we focused on the differentially expressed splicing factors in embryonic liver development and HCC. We ide...
Source: GEO: Gene Expression Omnibus - September 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE241894 Comparison of biochemical pathways affected by treatment with 4-methylumbelliferone and siRNA mediated knock down reveals hyaluronan synthase type 2 as a key pharmacological target in liver fibrosis
Contributors : Noreen Halimani ; Mikhail Nesterchuk ; Alexandra A Tsitrina ; Marat Sabirov ; Irina N Andreichenko ; Nataliya O Dashenkova ; Alexey M Kulikov ; Timofei S Zatsepin ; Roman A Romanov ; Arsen S Mikaelyan ; Yuri V KotelevtsevSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHepatic fibrosis is a morbid pathology with unmet medical need. 4- methylumbelliferone (4-MU), an inhibitor of hyaluronan (HA) synthesis was shown to be safe in phase one clinical trials. To support drug development, it is essential to decipher 4-MU ’s pharmacological targets. Here, we compared the effec...
Source: GEO: Gene Expression Omnibus - August 31, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE236523 CD44 is required for coupling autophagy decline and aging in the vascular endothelium
Contributors : Lu Zhang ; Peichang YangSeries Type : Expression profiling by arrayOrganism : Homo sapiensThe decline of endothelial autophagy is closely related to vascular senescence and the development of diseases; however, little is known about the molecular mechanism by which autophagy decline is associated with aging in vascular endothelial cells (VECs). Here we identify a crucial role for CD44, a multifunctional adhesion molecule that has been implicated as a cancer stem cell marker in several cancers, in controlling autophagy and aging in VECs. CD44 intercellular domain (CD44ICD) negatively regulates autophagy via a...
Source: GEO: Gene Expression Omnibus - August 30, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE241418 Transcriptomic and Translatomic Analyses Reveal Insights into the Signaling Pathways of the Innate Immune Response in the Spleens of SPF Chickens Infected with Avian Reovirus
This study not only provides a new perspective on the regulatory mechanisms of the innate immune response after ARV infection but also enriches the knowledge of the host defense mechanisms against ARV invasion and the outcome of ARV evasion of the host innate immune response.
Source: GEO: Gene Expression Omnibus - August 27, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Gallus gallus Source Type: research
GSE233807 RNA N6-methyladenosine methyltransferase METTL3 drives NAFLD-HCC and is a therapeutic target for boosting immunotherapy m6A-seq
Series Type : Methylation profiling by high throughput sequencingOrganism : Homo sapiensNon-alcoholic fatty liver disease (NAFLD) is an emerging risk factor of hepatocellular carcinoma (HCC). However, the mechanism and target therapy on NAFLD-HCC are still unclear. Here, we identify that the N6-methyladenosine (m6A) methyltransferase METTL3 promotes NAFLD-HCC. Hepatocyte-specific Mettl3 knockin exacerbated NAFLD-HCC formation while Mettl3 knockout exerted an opposite effect in mice. Single-cell RNA-seq revealed that METTL3 suppressed antitumor immune response by reducing infiltration of Gzmb+ and IFN- γ+ CD8+ T-cell, ther...
Source: GEO: Gene Expression Omnibus - August 23, 2023 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Homo sapiens Source Type: research
