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Source: GEO: Gene Expression Omnibus
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Total 772 results found since Jan 2013.
GSE115475 Effects of siRNA-mediated knockdownof RUNX1 expression in breast cancer cells
Contributors : Chin-Yo Lin ; Nicholes CandelariaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPrevious studies have identified an interaction between RUNX1 and estrogen receptor alpha and its potential role in estrogen signaling in breast cancer. To determine the transcriptomic actions of RUNX1, we knocked down its expression by using siRNAs, both in the absence and presence of estradiol (E2).
Source: GEO: Gene Expression Omnibus - June 1, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE163553 Gene expression data from human breast cancer MCF-7 cells treated with siRNAs targeting SCFD2
Contributors : Toshihiko Takeiwa ; Kazuhiro Ikeda ; Kuniko Horie-Inoue ; Satoshi InoueSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo examine the role of SCFD2 in breast cancer, MCF-7 cells were treated with siRNAs targeting SCFD2 (siSCFD2) or control siRNA (siControl). SCFD2 silencing represses expressions of anti-apoptotic genes which are positively associated with shorter relapse-free survival in ER-positive breast cancer patients. These results reveal the clinical and functional significance of SCFD2 and its targets in hormone-dependent breast cancer. SCFD2 may be applied to the cancer management a...
Source: GEO: Gene Expression Omnibus - May 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE197920 Ectopic histone modification in extra-embryonic tissues influence implantation and placenta development of IVF embryos ChIP-Seq 2
Contributor : Dandan BaiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusTo further investigate the relationship between abnormal accumulation of H3K4me3 modification and abnormal embryo development. We injected Kdm5b siRNA, which is an H3K4me3 eraser, at the zygote (PN3-PN4) stage of NM embryos and then examined the H3K4me3 modification of extraembryonic ectoderm at E7.5.
Source: GEO: Gene Expression Omnibus - May 26, 2022 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
GSE197914 Ectopic histone modification in extra-embryonic tissues influence implantation and placenta development of IVF embryos RNA-Seq 3
Contributor : Dandan BaiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo further investigate the relationship between abnormal accumulation of H3K4me3 modification and abnormal embryo development. We injected Kdm5b siRNA, which is an H3K4me3 eraser, at the zygote (PN3-PN4) stage of NM embryos and then examined the transcriptome of extraembryonic ectoderm at E7.5.
Source: GEO: Gene Expression Omnibus - May 26, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE151128 RNA-seq analysis of SCC1 and FaDu cells after FOSL1 depletion
Contributor : Jiong LiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn order to examine how FOSL1 affects the global gene transcriptome in HNSCC, we performed RNA-sequencing in SCC1 and FaDu cells treated with FOSL1 siRNA. Depletion of FOSL1 led to inhibition of cancer stemness genes.
Source: GEO: Gene Expression Omnibus - May 23, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE131709 RNA-sequencing-based off-target analysis of allele-specific siRNA in keratitis-ichthyosis-deafness syndrome patient-derived keratinocytes
Contributors : Ming Y Lee ; Tony Brooks ; Alan Pittman ; Veronica A Kinsler ; Wei L DiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: RNA-sequencing (RNA-Seq)-based transcriptomic analysis has facilitated identification of off-target effects of drug of interest. The goals of this study are to investigate off-target effects of the therapeutic, allele-specific siRNA, S7.Methods: mRNA profiles of KID syndrome patient-derived keratinocytes (with c.148G>A mutation in GJB2) with or without S7 treatment were generated by RNA-Seq, in triplicate, using an Illumina NextSeq 500 instrume...
Source: GEO: Gene Expression Omnibus - May 23, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE183013 Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of siRNA mediated ASGR1 knockdown
Contributors : Ju-Qiong Wang ; Bao-Liang SongSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: The goal of this study is to demonstrate the gene profiles of ASGR1 knock down mediated by siRNA compared with siControl in Huh7 cells.Methods: The Huh7 cells were transfected with small interference RNA (siRNA) targeting scramble (WT) or ASGR1 (AS2) for 8 hours, then cells were refreshed with Dulbecco's Modified Eagle Medium (DMEM) supplemented with 100 units ml-1 penicillin, 100 μg ml-1 streptomycin sulfate, and 10% fetal bovine serum (FBS). After 72 hours, the cells were harvested...
Source: GEO: Gene Expression Omnibus - May 19, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE178678 A genetics screen uncovers the role of RNA-binding protein RBP45D in flowering time under heat and DCL3-independent RNA-directed DNA methylation (WGBS)
Contributor : Tatjana KleineSeries Type : Methylation profiling by high throughput sequencingOrganism : Arabidopsis thalianaRNA-directed DNA methylation (RdDM) is a prominent pathway in plants to defend against invasive nucleic acids. In the canonical RdDM, 24-nt small interfering RNAs (siRNAs) are produced through DICER-LIKE 3 (DCL3). Here, we describe the Arabidopsis thaliana prors1 (LUC) transgenic system in which transcriptional gene silencing (TGS) is independent of DLC3. In a forward genetics screen performed with this system, already known components of RdDM and RNA-binding protein RBP45D were identified. RBP45D pro...
Source: GEO: Gene Expression Omnibus - May 13, 2022 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Arabidopsis thaliana Source Type: research
GSE176315 A genetics screen uncovers the role of RNA-binding protein RBP45D in flowering time under heat and DCL3-independent RNA-directed DNA methylation
Contributor : Tatjana KleineSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaIn the canonical RdDM, small interfering RNAs (siRNAs) are produced through the slicer activity of DICER-LIKE 3 (DCL3). Here, we describe the Arabidopsis thaliana prors1 (LUC) transgenic system in which transcriptional gene silencing (TGS) through RdDM is independent of DLC3. To identify players in the DCL3-independent pathway, a forward genetics screen was performed with the prors1 (LUC) system to identify suppressor mutants in which TGS is released. Among already known components of RdDM, we identifi...
Source: GEO: Gene Expression Omnibus - May 13, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Source Type: research
GSE196499 Gene expression (GeneChip) data from scramble- and CRMP2A siRNA-treated A549 cells.
Contributors : Aristeidis E Boukouris ; Evangelos D MichelakisSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe sought to assess how temporary loss of the microtubule-associated protein CRMP2A could affect global gene expression in the A549 lung cancer cells.We used microarrays to identify differentially regulated genes when CRMP2A is temporarily removed from A549 cells using siRNA.
Source: GEO: Gene Expression Omnibus - May 1, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE179843 Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding
Contributors : Zhongxia Yi ; Rene M Arvola ; Sean Myers ; Corinne N Dilsavor ; Rabab Abu Alhasan ; Bayley N Carter ; Robert D Patton ; Ralf Bundschuh ; Guramrit SinghSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensNonsense-mediated mRNA decay (NMD) can occur independently of some NMD factors such as UPF3B. We have performed total RNA-Seq in HCT116 cells under NMD factor knockout (with CRISPR-Cas9) and/or knockdown (with siRNA transfection) conditions to identify mRNA substrates regulated by different NMD factors. We have also performed RIPiT-Seq to identify the footprints of t...
Source: GEO: Gene Expression Omnibus - April 23, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Homo sapiens Source Type: research
GSE200983 Effect of O-GlcNAc Transferase (OGT) siRNA in trophoblastic BeWo cells
Contributors : Stephanie Olivier-Van Stichelen ; Yiwen CuiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensEvery year, about 18 million babies are born from mothers with gestational diabetes mellitus (GDM). While diabetic symptoms usually resolve after delivery, lasting complications can occur for both mother and child, including fetal overgrowth, type 2 diabetes (T2D), cardiovascular diseases, and obesity. The rapid progression of GDM is unique to pregnancies, and likely arises from placental dysfunction. Indeed, stress, nutrition and fetal gender are thought to trigger changes in pla...
Source: GEO: Gene Expression Omnibus - April 21, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE149022 Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells tranfected with non-specific control (NSC) or ZAP-70 siRNA
Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
Source: GEO: Gene Expression Omnibus - April 19, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE149021 Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells tranfected with non-specific control (NSC) or ZAP-70 siRNA (+anti-IgM dataset)
Contributors : Jingyu Chen ; Vijitha Sathiaseelan ; Arash Shahsavari ; Irina Mohorianu ; Ingo RingshausenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA seq profiles of primary CLL cells transfected with non-specific control (NSC) or ZAP-70 siRNA ± anti-IgM. This submission is +anti-IgM.
Source: GEO: Gene Expression Omnibus - April 19, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE149020 Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells tranfected with non-specific control (NSC) or ZAP-70 siRNA (-anti-IgM dataset)
Contributors : Jingyu Chen ; Vijitha Sathiaseelan ; Arash Shahsavari ; Irina Mohorianu ; Ingo RingshausenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA seq profiles of primary CLL cells transfected with non-specific control (NSC) or ZAP-70 siRNA ± anti-IgM. This submission is -anti-IgM.
Source: GEO: Gene Expression Omnibus - April 19, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
