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Source: GEO: Gene Expression Omnibus

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Total 772 results found since Jan 2013.

GSE189430 Evolutionary transcriptomics of Eutherian gene expression gains and the origins of decidualization Set 2
Contributors : Mirna Marinic ; Vincent J LynchSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe used human endometrial stromal fibroblast cell line to perform an siRNA knockdown coupled with RNA-seq screen of a series of transcription factors that gained expression in the endometrial tissue of Eutherian mammals.
Source: GEO: Gene Expression Omnibus - February 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE189390 Evolutionary transcriptomics of Eutherian gene expression gains and the origins of decidualization Set 1
Contributors : Mirna Marinic ; Vincent J LynchSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe used human endometrial stromal fibroblast cell line to perform an siRNA knockdown coupled with RNA-seq screen of a series of transcription factors that gained expression in the endometrial tissue of Eutherian mammals.
Source: GEO: Gene Expression Omnibus - February 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE197376 Identifying target genes for the long noncoding RNA, GATA2-AS1
Contributors : H J Man ; Noeline Subramaniam ; Philip A MarsdenSeries Type : Expression profiling by array ; Non-coding RNA profiling by arrayOrganism : Homo sapiensWe have identified a set of endothelial-enriched long noncoding RNAs (lncRNAs) in another experiment. In this experiment, we aim to identify genes that are regulated by the GATA2-AS1 lncRNA. We have depleted GATA2-AS1 using two distinct siRNA in human umbilical vein endothelial cells (HUVEC)
Source: GEO: Gene Expression Omnibus - February 25, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Non-coding RNA profiling by array Homo sapiens Source Type: research

GSE175629 CMTR1 is recruited to transcription start sites and has enhanced influence over ribosomal protein and histone genes RNA-seq
Contributors : Shang Liang ; Victoria H CowlingSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe investigated the gene-specificity of mRNA cap methyltransferase CMTR1 and its impact in RNA expression. In this RNA-seq experiment, we used siRNA to knockdown the endogenous CMTR1 protein to investigate its role in mouse embryonic stem cells. We demonstrated that the repression of CMTR1 expression resulted in a loss of RNA expression, particularly of histone and ribosomal protein genes.
Source: GEO: Gene Expression Omnibus - February 25, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE197107 Negative autoregulation of BMP dependent transcription by SIN3B splicing reveals a role for RBM39
Contributors : Noel Faherty ; Shoumo Bhattacharya ; John Broxholme ; Eshita SharmaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe conducted RNASeq using mRNA extracted from C2C12 mouse myoblast cells transfected with non-targeting control (NTC) or RBM39 siRNA, to elucidate the effect of RBM39 on regulation of BMP4 pathway
Source: GEO: Gene Expression Omnibus - February 22, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE196262 DUB1 suppresses Hippo signaling via modulating TAZ protein in gastric cancer
Contributors : Dehai Wang ; Yinlu Ding ; Jian Zhu ; Peng SuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe Hippo pathway functions as a tumor-suppressor pathway in human cancers, while the dys-function of Hippo pathway is frequently observed in malignancies. Although the YAP/TAZ activity is tightly controlled by the phosphorylation cascade of MST-LATS-YAP/TAZ axis, it is still unclear why YAP/TAZ protein is activated in human cancers, even Hippo pathway is still active. Besides phosphorylation, recent studies implicate that several post-translational modifications also play critic...
Source: GEO: Gene Expression Omnibus - February 12, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE185668 RNAseq analyses of nucleus pulposus cells treated with control siRNA and LINC02569 siRNA
Contributors : Fuxin Wei ; Yulin HuangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensNucleus pulposus cells (NPCs) were used to examine the effect of LINC02569, a novel lncRNA. In particular, its effect in intervertebral disc degeneration was determined. Treatment of LINC02569 siRNA (si-02569) was to be compared with negative control siRNA (si-NC).
Source: GEO: Gene Expression Omnibus - February 8, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE176464 Regulation of alternative splicing by a novel BRR2 interactor C9ORF78
We present cryo-EM structures showing how C9ORF78 and the spliceosomal B-complex protein FBP21 wrap around the C-terminal helicase cassette of BRR2 and that binding of the two proteins is mutually exclusive. C9ORF78 associates with the spliceosome, as we confirm via proteomics and RNA UV-crosslinking. An siRNA mediated C9ORF78 knockdown reveals changes in alternative splicing of specific target pre-mRNAs, which in part depend on its interaction with BRR2. In particular, C9ORF78 regulates a substantial number of alternative 3 ’ splice sites, which might be facilitated through an additional interaction with human PRP22 (DHX8).
Source: GEO: Gene Expression Omnibus - February 4, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE169171 Analysis of mRNA-derived siRNAs in mutants of mRNA maturation and surveillance pathways in Arabidopsis thaliana
Contributors : Michal Krzyszton ; Joanna KufelSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thalianaDefects in RNA maturation and RNA decay factors may generate substrates for the RNA interference machinery. This phenomenon was observed in plants where mutations in some RNA-related factors lead to the production of RNA-quality control small interfering RNAs and several mutants show enhanced silencing of reporter transgenes. To assess the potential of RNAi activation on endogenous transcripts, we sequenced small RNAs from a set of Arabidopsis thaliana mutants with defects in vari...
Source: GEO: Gene Expression Omnibus - February 2, 2022 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE195668 Lariat mapping of wild type and DBR1 KO cells after knockdown of AQR
Contributors : Jing Wang ; Luke Buerer ; Rachel Soemedi ; William FairbrotherSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensUsing wild type HEK293T cells and our DBR1 CRISPR knockout cell line (C22), we investigated the effect of AQR knockdown on cellular lariat levels. Samples were taken after transfection with either one of two AQR-targeting siRNAs or a non-targeting control siRNA. After sequencing the samples, lariat mapping of the reads revealed that AQR knockdown increased the lariat recovery rate by ~50% relative to the level observed in the negative control samples.
Source: GEO: Gene Expression Omnibus - January 31, 2022 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research

GSE192804 ISG 15 is associated with cervical cancer development
Contributors : Pingping Tao ; Liyan Sun ; Yanmei Sun ; Binlie YangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCervical cancer (CC) is a complex disease. Many factors contribute to the tumourigenesis and progression of CC neoplasms. Here, we analysed transcriptomic differences and simulated tumour progression to explore the pathogenesis of CC.RNA-seq was performed to analyse the transcriptomic differences among normal tissue (NC), paracarcinoma tissue (TP), and primary tumour tissue (TT). Pseudo-time analysis was performed to simulate tumour progression. RT-qPCR and immunohistochem...
Source: GEO: Gene Expression Omnibus - January 2, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE189362 Regulation of alternative splicing by C9ORF78, a novel BRR2 interactor II
We present cryo-EM structures showing how C9ORF78 and the spliceosomal B-complex protein FBP21 wrap around the C-terminal helicase cassette of BRR2 and that binding of the two proteins is mutually exclusive. C9ORF78 associates with the spliceosome, as we confirm via proteomics and RNA UV-crosslinking. An siRNA mediated C9ORF78 knockdown reveals changes in alternative splicing of specific target pre-mRNAs, which in part depend on its interaction with BRR2. In particular, C9ORF78 regulates a substantial number of alternative 3 ’ splice sites, which might be facilitated through an additional interaction with human PRP22 (DHX8).
Source: GEO: Gene Expression Omnibus - December 31, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE184859 Brusatol inhibits proliferation and invasion of glioblastoma by down-regulating the expression of ECM1
Contributors : Cai Lin ; Dai Zhang'an ; Lu XiangheSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBrusatol (Bru), a Chinese herbal extract, has a variety of anti-tumor effects. However, little is known regarding its role and underlying mechanism in glioblastoma cells. Here, we found that Bru could inhibit the proliferation of glioblastoma cells in vivo and in vitro. Besides, it also had an inhibitory effect on human primary glioblastoma cells. In order to further study the underlying mechanism of Bru ’s action on GBM, we performed RNA sequencing array on GBM cells treated by Bru wit...
Source: GEO: Gene Expression Omnibus - December 31, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE181089 IL-37 negatively regulates IL-33 expression in human keratinocytes
Contributors : Gaku Tsuji ; Akiko Hashimoto-Hachiya ; Tomoyo Matsuda-Taniguchi ; Ayako Takai-Yumine ; Masaki Takemura ; Xianghong Yan ; Masutaka Furue ; Takeshi NakaharaSeries Type : Expression profiling by arrayOrganism : Homo sapiensInterleukin (IL)-37 suppresses systemic and local inflammation. It is expressed in the epidermis, the external layer of the skin, and is decreased in inflammatory skin diseases including atopic dermatitis (AD) and psoriasis. Therefore, an agent applied topically on the skin that can increase IL-37 could be promising for treating AD and psoriasis; however, the mechanism regulating IL-37 remain...
Source: GEO: Gene Expression Omnibus - December 31, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE180248 Transcriptomic analysis of HDAC6-selective and broad HDAC inhibitors in human WT iPSC cardiomyocytes
Contributors : Jin Yang ; Francis Grafton ; Ana Budan ; Sara Ranjbarvaziri ; Farshad Farshidfar ; Marie Cho ; Emma Xu ; Jaclyn Ho ; Mahnaz Maddah ; Kevin E Loewke ; Julio Medina ; David Sperandio ; Snahel Patel ; Tim Hoey ; Mohammad A MandegarSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDilated cardiomyopathy (DCM) is characterized by reduced cardiac output, as well as thinning and enlargement of left ventricular chambers. These characteristics eventually lead to heart failure. Current standards of care do not target the underlying molecular mechanisms associated with genetic forms...
Source: GEO: Gene Expression Omnibus - December 31, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research