Filtered By:
Source: GEO: Gene Expression Omnibus
This page shows you your search results in order of date. This is page number 19.
Order by Relevance | Date
Total 772 results found since Jan 2013.
GSE167945 IRF4 genome wide binding and transciptomic profile in 501Mel melanoma cell line
Contributors : Ramile Dilshat ; Berglind O Einarsd óttirSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensTo map genome wide binding sites of IRF4, we performed ChIP-seq against eGFP tagged IRF4 in human melanoma cells. To assess whether IRF4 binding influences its target gene expression, RNA-seq was performed after siRNA mediated depletion of IRF4 in 501Mel cells. Subsequently, target genes of IRF4 obtained from ChIP-seq and RNA-seq studies were used to interrogate melanocyte meQTL and identified 131 candidate downstre...
Source: GEO: Gene Expression Omnibus - March 2, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
GSE159839 siRNA mediated HDAC1 knockdown in the LNCaP prostate cancer cell line
Contributors : Simone Bauer ; Holger S ültmannSeries Type : Expression profiling by arrayOrganism : Homo sapienssiRNA mediated HDAC1 knockdown in the LNCaP prostate cancer cell line.
Source: GEO: Gene Expression Omnibus - March 1, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE167440 Quantitative Analysis of Scramble control and LncRNA HMRHL siRNA knockdown in chronic myelogenous leukemia (CML) K562 Cell lines transcriptome profile
Contributors : Subhendu R Choudhury ; Sangeeta Dutta ; Utsa Bhaduri ; Satyanarayana RaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe goals of this study are to compare differentianl gene expression between Scramble control and knockdown of HMRHL in K562 cell lines. Subsequently to determine the cellular pathway and biological process affected most upon HMRHL silencing from NGS-derived K562 cells transcriptome profiling (RNA-seq).
Source: GEO: Gene Expression Omnibus - March 1, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE167590 SOX9 modulates cancer biomarker and cilia genes in pancreatic cancer
We report 93 differentially expressed (DE) genes, with convergence on alterations to Notch-signaling pathways and ciliogenesis. These results point to SOX9 and Notch activity being in a positive feedback loop; and, SOX9 regulating cilia production in PDAC. We additionally performed ChIP-seq in PANC-1 cells to identify direct targets of SOX9 binding and integrated these results with our DE gene list. Nine of the top ten downregulated genes have evidence of direct SOX9 binding at their promoter regions. One of these targets was the cancer stem cell marker EpCAM. Using whole-mount in situ hybridization to detect epcam ...
Source: GEO: Gene Expression Omnibus - February 27, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
GSE138991 Caveolin1 Deficiency: A Type I Endothelial Interferonopathy Associated with Pulmonary Arterial Hypertension
Contributors : Salina Gairhe ; Gabriela A Ferreyra ; Jason M Elinoff ; Cumhur Y Demirkale ; Robert L DannerSeries Type : Expression profiling by arrayOrganism : Homo sapiensCAV1 loss-of-function mutations have been associated with the development of pulmonary arterial hypertension (PAH). CAV1 is an integral component of endothelial caveolae, specialized lipid rafts that attach to the actin cytoskeleton and modulate receptor/signal transduction coupling. CAV1 loss in pulmonary artery endothelial cells produced a proliferative, hypermigratory cellular phenotype with a disrupted cytoskeletal architecture, mirroring known feat...
Source: GEO: Gene Expression Omnibus - February 24, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE167197 Peroxisome import stress induces eIF2 α phosphorylation and impairs ribosome biogenesis
Contributors : Kerui Huang ; Jinoh Kim ; Pham Vo ; Ting Miao ; Mirre J Simons ; Hua BaiSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogaster ; Homo sapiensPeroxisome biogenesis diseases (PBDs) are characterized by global defects in peroxisomal function and can result in severe brain, liver, kidney, and bone malfunctions. PBDs are due to mutations in peroxisome biogenesis factors (PEX genes) that are responsible for peroxisome assembly and function. Increasing evidence suggests that peroxisome import functions decline during aging. However, the transcriptome profiling of peroxiso...
Source: GEO: Gene Expression Omnibus - February 23, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila melanogaster Homo sapiens Source Type: research
GSE163304 Mapping of p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1)
Contributors : Fabienne Bejjani ; Marc Piechaczyk ; Isabelle Jariel-EncontreSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensIn the article "Fra-1 regulates its target genes via binding to remote enhancers without exerting major control on chromatin architecture in triple negative breast cancers" by Bejjani et al., we mapped p300/CBP binding sites in MDA-MB-231 cells transfected with a control siRNA or a siRNA directed against Fra-1(FOSL1) to study whether Fra-1 can modulate p300/CBP recruitment on MDA-MB-231 genome
Source: GEO: Gene Expression Omnibus - February 23, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
GSE136190 RNA-seq of adherent MDA-MB-231 cells, and their 16hour and 5day mammospheres and siRNA treatment
Contributors : Sladjana Zagorac ; Leandro CastellanoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo examine the transcriptional landscape of breast cancer stem cells enriched population in triple negative breast cancer, we compared transcript change between adherent MDA-MB-231 cells and the same cells cultured in mammosphere conditions for 16 hours or 5 days. Mammosphere assay is a recognised in vitro model of the breast cancer stem cells , and we wished to identify transcriptional dynamics and novel noncoding RNAs implicated in the regulation of this cell type. To define the role ...
Source: GEO: Gene Expression Omnibus - February 23, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE136185 Long non coding RNA (lncRNA) SCIRT regulates mitotic and stemness genes in breast cancer stem cells
Contributors : Sladjana Zagorac ; Leandro CastellanoSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensCHART sequencing has been carried out in order to evaluate SCIRT target genes in MDA-MB-231 sphere derived cells. We discovered that lncRNA SCIRT binds promoter region of genes whose expression changes after SCIRT siRNA downregulation, suggesting that those are target SCIRT target genes.
Source: GEO: Gene Expression Omnibus - February 23, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
GSE162966 IL11 is elevated in systemic sclerosis and IL11-dependent ERK signaling is needed for the profibrotic activity of TGF β in dermal fibroblasts
Conclusions: These data reveal an important contribution of IL11-related ERK activity for TGF β-stimulated fibrosis phenotypes in HDFs and suggest IL11 as a potential therapeutic target in SSc.
Source: GEO: Gene Expression Omnibus - February 22, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE166823 Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes
We report gene expression changes after knockdown of transcription factors in human iPSC-derived cardiac myocytes. In prior experiments we showed that transcription factors known to be important for cardiac development were frequently up-regulated (i.e., "responsive") after small molecule perturbations of cultured iPSC-derived cardiac myocytes. We used RNA sequencing to test whether siRNA-mediated knockdown of transcription factors with different perturbation-responsiveness and tissue-specificity profiles would lead to inappropriate up-regulation of non-myocyte gene sets in cardiac myocytes.
Source: GEO: Gene Expression Omnibus - February 17, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE139455 Identification of STAT3 downstream targets involved in chemoradiotherapy resistance
Contributors : Kristin K ördel ; Melanie Spitzner ; Marian Grade ; Jürgen Wienands ; Andreas LehaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-Seq data of human colorectal cancer cell line SW837 transfected with siRNA against STAT3 (siSTAT3) or control RNA (siCtrl), untreated or treated with Hyper-IL-6 (Hy-IL-6)
Source: GEO: Gene Expression Omnibus - January 25, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE165112 Interferon-induced transmembrane protein 1 (IFITM1) inhibits Varicella-Zoster Virus Replication
Contributors : Seong K Kim ; Akhalesh K Shakya ; Dennis J O'CallaghanSeries Type : Expression profiling by arrayOrganism : Homo sapiensHosts have evolved numerous mechanisms to prevent primary viral infections. Interferon signaling is an important host defense mechanism against primary infection. Interferon gamma (IFN- γ) is a potent cytokine produced following primary varicella-zoster virus (VZV) infection. Furthermore, VZV reactivation correlates with a decline in IFN-γ-producing immune cells. Our previous results showed that pretreatment with 20 ng/ml of IFN-γ completely inhibited VZV replication in lung fib roblast ...
Source: GEO: Gene Expression Omnibus - January 20, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE165078 Antisense ribosomal siRNAs inhibit RNA polymerase I-directed transcription in C. elegans
Contributor : Shouhong GuangSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Caenorhabditis elegansEukaryotic cells express a wide variety of endogenous small regulatory RNAs that function in the nucleus. We previously found that erroneous rRNAs induce the generation of antisense ribosomal siRNAs (risiRNAs) which silence the expression of rRNAs via the nuclear RNAi defective (Nrde) pathway. To further understand the biological roles and mechanisms of this class of small regulatory RNAs, we conducted forward genetic screening to identify factors involved in risiRNA generation in Caenorhabditis ...
Source: GEO: Gene Expression Omnibus - January 20, 2021 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Caenorhabditis elegans Source Type: research
GSE162349 mRNA Sequencing of control and huntington's disease iPSC-derived medium spiny neuron-like cells and Q175 HET or WT mice. Plus and minus knockdown of PIAS1.
Contributors : Ryan G Lim ; Jie Wu ; Leslie M ThompsonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusHD and control patient-derived induced pluripotent stem cells were used to generate medium spiny neuron-like cells. four control in duplicate and three HD samples in duplicate with CAG repeat length in juvenile onset range were differentiated as biological growth replicates (separate differentiations) into medium spiny neuron-like cells. Cells were treated with LNPs with siRNA for knockdown of PIAS1 or a luciferase control. Total RNA was isolated using the Qiagen RNeasy...
Source: GEO: Gene Expression Omnibus - January 1, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Mus musculus Source Type: research
