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Source: GEO: Gene Expression Omnibus
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Total 772 results found since Jan 2013.
GSE87497 Evaluating Side Effects of Nanoparticle-mediated siRNA Delivery to Mesenchymal Stem Cells Using Next-generation Sequencing and Enrichment Analysis
This study investigates both intended and unintended effects of diblock copolymer nanoparticle (NP) delivery of siRNA delivery to human mesenchymal stem cells (hMSC). Specifically, siRNA delivery was investigated at a range of NP-siRNA:hMSC ratios with a focus on the effects of NP-siRNA treatment on hMSC functions. Additionally, next-generation RNA sequencing (RNA-seq) was used with enrichment analysis to observe side effects in hMSC gene expression. Results show NP-siRNA delivery is negatively correlated with hMSC density. However, higher NP-siRNA:hMSC ratios increased cytotoxicity and decreased metabolic activity. hMSC p...
Source: GEO: Gene Expression Omnibus - September 30, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE194248 Human prostate cancer cells (VCaP and AIVCaP): siAR alone (single siRNA) vs. siAR plus OPRK1 (combo-siRNA)
Contributors : Yuki Makino ; Takashi KobayashiSeries Type : Expression profiling by arrayOrganism : Homo sapiensThrough comparative genomics using PDX models of androgen-dependent (AD) and castration-resistant (CR) tumors, we identify opioid receptor kappa 1 (OPRK1) as being associated with castration-resistance. Loss of OPRK1 function delays castration-resistance and inhibits castration-resistant growth of prostate cancer cells in culture and in vivo, suggesting OPRK1 as a therapeutic target.To gain insight with regard to biological function of OPRK1 under androgen-depleted condition, we set up two distinct comparisons us...
Source: GEO: Gene Expression Omnibus - April 3, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE65070 The hub protein Loqs connects the miRNA and endo-siRNA pathways in mosquitos
Contributors : Mary E Haac ; Michelle A Anderson ; Heaher L Eggleston ; Kevin M Myles ; Zach N AdelmanSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Aedes aegyptiAedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided...
Source: GEO: Gene Expression Omnibus - April 8, 2015 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Aedes aegypti Source Type: research
GSE57215 Detection of Pol IV-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis
Contributors : Shaofang Li ; Xuemei ChenSeries Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thaliana24 nucleotide siRNAs are central players in RNA-directed DNA methylation (RdDM), a process that establishes DNA methylation at transposable elements to ensure genome stability. The plant-specific RNA polymerase IV (Pol IV) is required for siRNA biogenesis and is thought to transcribe RdDM loci to produce primary transcripts that serve as precursors to siRNAs. Yet, no such transcripts have ever been reported. Here, through RNA sequenci...
Source: GEO: Gene Expression Omnibus - November 11, 2014 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research
GSE140729 Structure of LINC00511-siRNA-conjugated nanobubbles and improvement of cisplatin sensitivity on triple negative breast cancer
Contributors : Bolin Wu ; Yanchi Yuan ; Erjing Li ; Hui Jing ; Wen ChengSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn the present study, we prepared a novel theranostic agent loaded with LINC00511-siRNA, which not only can serve as the siRNA delivery platform but also as the UCA for contrast enhanced ultrasound (CEUS). Combined with low-frequency ultrasound exposure, the novel theranostic agent achieves the controlled release of LINC00511-siRNA and facilitates the transfection of LINC00511-siRNA. Furthermore, in order to investigate how LINC00511 affects drug resistance of TNBC, ...
Source: GEO: Gene Expression Omnibus - September 3, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE158710 The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice
Contributors : Kezhi Zheng ; Dong-Lei YangSeries Type : Methylation profiling by high throughput sequencingOrganism : Oryza sativaRNA-directed DNA methylation (RdDM) functions in de novo methylation in CG, CHG, and CHH contexts. Here, we performed map-based cloning of OsNRPE1, which encodes the largest subunit of Pol V, a key regulator of gene silencing and reproductive development in rice. We found that rice Pol V is required for CHH methylation on RdDM loci by transcribing long non-coding RNAs. Pol V influences the accumulation of 24-nt siRNAs in a locus-specific manner. Biosynthesis of 24-nt siRNAs on loci with high CHH...
Source: GEO: Gene Expression Omnibus - July 7, 2021 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Oryza sativa Source Type: research
GSE158709 The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice
Contributors : Kezhi Zheng ; Dong-Lei YangSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Oryza sativaRNA-directed DNA methylation (RdDM) functions in de novo methylation in CG, CHG, and CHH contexts. Here, we performed map-based cloning of OsNRPE1, which encodes the largest subunit of Pol V, a key regulator of gene silencing and reproductive development in rice. We found that rice Pol V is required for CHH methylation on RdDM loci by transcribing long non-coding RNAs. Pol V influences the accumulation of 24-nt siRNAs in a locus-specific manner. Biosynthesis of 24-nt siRNAs on loci with high ...
Source: GEO: Gene Expression Omnibus - July 7, 2021 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Oryza sativa Source Type: research
GSE152863 MCF10A cells expressing empty vector, siRNA-resistant wild-type and siRNA-resistant catalytically dead TIP60 treated with control or TIP60 siRNA
This study suggests that alternative s plicing of ITGA6 exon 25 is regulated by the TIP60-ESRP2 axis.
Source: GEO: Gene Expression Omnibus - June 19, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE54146 human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected
Contributors : Tatsuo Miyamoto ; Makoto Furutani-SeikiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA.
Source: GEO: Gene Expression Omnibus - February 1, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE66662 Analysis of global gene expression profiles of A549 cells treated with XRN2 siRNA under basal conditions
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or XRN2 siRNA treated A549 cells were analyzed for gene expression differences under basal conditions
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE66661 Analysis of global gene expression profiles of A549 cells treated with Xrn2 siRNA upon Influenza A/PR/8/34(ΔNS1) Infection
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or Xrn2 siRNA treated A549 cells were infected with A/Puerto Rico/8/1934(ΔNS1) for 4 hours
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE52933 Analysis of global gene expression profiles of A549 cells treated with SETX siRNA under basal conditions
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or SETX siRNA treated A549 cells were analyzed for gene expression differences under basal conditions
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE52932 Analysis of global gene expression profiles of A549 cells treated with SETX siRNA upon Influenza A/PR/8/34(ΔNS1) Infection
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana M Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or SETX siRNA treated A549 cells were infected with A/Puerto Rico/8/1934(ΔNS1) for 4 hours
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE70914 HEK293T cells treated with a G9a small molecule inhibitor (UNC0638) or vehicle (DMSO), WIZ siRNA, G9a siRNA, or scrambled (control) siRNA
Contributors : Samantha G Pattenden ; Joel S ParkerSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptional profiling of HEK293T cells following treatment with a small molecule inhibitor of catalytic activity of the G9a lysine methyltransferase (UNC0638, 1 micromolar, 48 hours), or siRNA against WIZ or G9a (72 hours treatment).
Source: GEO: Gene Expression Omnibus - July 15, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE72485 Human pulmonary microvascular endothelial cells (HPMECs): Control siRNA vs HSP27 siRNA
Contributors : Seo H Choi ; Yoon-Jin LeeSeries Type : Expression profiling by arrayOrganism : Homo sapiensTranscriptional profiling of HPMECs comparing Control siRNA treated with HSP27 siRNA
Source: GEO: Gene Expression Omnibus - August 29, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
