GSE179843 Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding

Contributors : Zhongxia Yi ; Rene M Arvola ; Sean Myers ; Corinne N Dilsavor ; Rabab Abu Alhasan ; Bayley N Carter ; Robert D Patton ; Ralf Bundschuh ; Guramrit SinghSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensNonsense-mediated mRNA decay (NMD) can occur independently of some NMD factors such as UPF3B. We have performed total RNA-Seq in HCT116 cells under NMD factor knockout (with CRISPR-Cas9) and/or knockdown (with siRNA transfection) conditions to identify mRNA substrates regulated by different NMD factors. We have also performed RIPiT-Seq to identify the footprints of three compositionally distinct EJCs that contain the following pairs of proteins: MAGOH-EIF4A3, UPF3B-EIF4A3 or CASC3-EIF4A3 in WT HCT116 cells.

Source: GEO: Gene Expression Omnibus - April 23, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Homo sapiens Source Type: research